VAMP2 (NM_014232) Human Untagged Clone

CAT#: SC115104

VAMP2 (untagged)-Human vesicle-associated membrane protein 2 (synaptobrevin 2) (VAMP2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VAMP2
• Synonyms: SYB2; VAMP-2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115104 sequence for NM_014232 edited (data generated by NextGen Sequencing)
  ATGTCTGCTACCGCTGCCACGGCCCCCCCTGCTGCCCCGGCTGGGGAGGGTGGTCCCCCT
  GCACCCCCTCCAAACCTCACCAGTAACAGGAGACTGCAGCAGACCCAGGCCCAGGTGGAT
  GAGGTGGTGGACATCATGAGGGTGAACGTGGACAAGGTCCTGGAGCGAGACCAGAAGCTG
  TCGGAGCTGGACGACCGTGCAGATGCACTCCAGGCGGGGGCCTCCCAGTTTGAAACAAGC
  GCAGCCAAGCTCAAGCGCAAATACTGGTGGAAAAACCTCAAGATGATGATCATCTTGGGA
  GTGATTTGCGCCATCATCCTCATCATCATCATAGTTTACTTCAGCACTTAA
  
  Clone variation with respect to NM_014232.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014232 unedited
  TCTTGGCATATTTGNAATCACGACTTCACTATAGGNCGGCACGCGCAATTCGGCACGAGG
  CCAGTCGGAGCCGCGCGAGCCGCCGCCGCCATCACTGCCGCTGCCAAGTCCTCCACCCGC
  TGCCCCCGCCATGTCTGCTACCGCTGCCACGGCCCCCCCTGCTGCCCCGGCTGGGGAGGG
  TGGTCCCCCTGCACCCCCTCCAAACCTCACCAGTAACAGGATACTGCAGCAGACCCAGGC
  CCATGTGGATGAGGTGGTGGACATCATGAGGGTGAACGTGGACAAGGTCCTGGAGCGAGA
  CCAGAAGCTGTCGGAGCTGGACGACCGTGCAGATGCACTCCAGGCGGGGGCCTCCCAGTT
  TGAAACAAGCGCAGCCAAGCTCAAGCGCAAATACTGGTGGAAAAACCTCAAGATGATGAT
  CATCTTGGGAGTGATTTGCGCCATCATCCTCATCATCATCATAGTTTACTTCAGCACTTA
  AATCCCCGAGGAGTCTGCCCTGCCTAGAGAAGGGCCTCTCCCCCAACCCTCAGCCGTTCC
  TCCACCTCTCAGCCATATCTTTCAGCCCCCCCTCCCCTGGATCCGTGTGTGTGTGTGTCC
  GTGTGTGTGTCCCCCTGTAAATAGCCAGCTGTTATTTATACATATATAATATTATATATA
  TTTGGTCTGTTTGTAGTTTTATTACTAGATGATTTTTCCGGTTGTCCTTAACACCCCTTC
  CTGAGGTTCCCTTCACCCCTCTCTCTTGCCTTACTTCCCTTTCCCTTTCTTCCTGACTAG
  CCCCAAAGTCCCTTCATTTGCATCTGCTATGCAATAGTCCCTCTCCTTTCCTTCTNCTNC
  CCTCAGATTTAGCTGATCCTTCCTNCCACCCTGGNCCTTCCTTTNCTCTTTNCTNCTNAC
  TCTNCCCGTCATGCTCCCTCTGCCCCGGCCTNNAAAANANNANNAANNNNANNNNNNNNN
  NANAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014232
• Insert Size: 1190 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014232.1, NP_055047.1
• RefSeq Size: 2159 bp
• RefSeq ORF: 351 bp
• Locus ID: 6844
• Cytogenetics: 17p13.1
• Domains: synaptobrevin
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: SNARE interactions in vesicular transport
• Gene Summary: 'The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes isoform 1.