NDUFA8 (NM_014222) Human Untagged Clone

CAT#: SC115137

NDUFA8 (untagged)-Human NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 8, 19kDa (NDUFA8), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NDUFA8
• Synonyms: CI-19KD; CI-PGIV; PGIV
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_014222 edited
  ATGCCGGGGATAGTGGAGCTGCCCACTCTAGAGGAGCTGAAAGTAGATGAGGTGAAAATT
  AGTTCTGCTGTGCTTAAAGCTGCGGCCCATCACTATGGAGCTCAATGTGATAAGCCCAAC
  AAGGAATTTATGCTCTGCCGCTGGGAAGAGAAAGATCCGAGGCGGTGTTTAGAGGAAGGC
  AAACTGGTCAACAAGTGTGCTTTGGACTTCTTTAGGCAGATAAAACGTCACTGTGCAGAG
  CCTTTTACAGAATATTGGACTTGCATTGATTATACTGGCCAGCAGTTATTTCGTCACTGT
  CGCAAACAGCAGGCAAAGTTTGACGAGTGTGTGCTGGACAAACTGGGCTGGGTGCGGCCT
  GACCTGGGAGAACTGTCAAAGGTCACCAAAGTGAAAACAGATCGACCTTTACCGGAGAAT
  CCCTATCACTCAAGACCAAGACCGGATCCCAGCCCTGAGATCGAGGGAGATCTGCAGCCT
  GCCACACATGGCAGCCGCTTTTATTTCTGGACCAAGTAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014222 unedited
  GTTTGNAATTTGTAATACGACTCACTATAGGNGCGGNNCGCGAATCGGCACGAGGCGGGG
  GCGACGCGGCTGAGGGCTTCTCGTCGGGGTCGGGGCTGCAGCCGTCATGCCGGGGATAGT
  GGAGCTGCCCACTCTAGAGGAGCTGAAAGTAGATGAGGTGAAAATTAGTTCTGCTGTGCT
  TAAAGCTGCGGCCCATCACTATGGAGCTCAATGTGATAAGCCCAACAAGGAATTTATGCT
  CTGCCGCTGGGAAGAGAAAGATCCGAGGCGGTGTTTAGAGGAAGGCAAACTGGTCAACAA
  GTGTGCTTTGGACTTCTTTAGGCAGATAAAACGTCACTGTGCAGAGCCTTTTACAGAATA
  TTGGACTTGCATTGATTATACTGGCCAGCAGTTATTTCGTCACTGTCGCAAACAGCAGGC
  AAAGCTTGACGAGTGTGTGCTGGACAAACTGGGCTGGGTGCGGCCTGACCTGCGAGAACT
  GCCAAAGGTCACCAAAGTGAAAACAGATCGACCTTTACCGGAGAATCCCTATCACTCAAG
  ACCAAGACCGGATCCCAGCCCTGAGATCGAGGGAGATCTGCAGCCTGCCACACATGGCAG
  CCGCTTTTATTTCTGGACCAAAGCAAAGATGGGTCCGTGGCCCACACTCGGGTCATGTGC
  TCAGAACACGACTGATGAAAACCGCCCATGCGGTTTGCATCGACTGATAGTGCGCTCCTT
  TCCGGGACACAAACATTAACAAAAAATTAACCTATGTGACTTGGCCGTTATTCCATACCA
  TCTCCTCGCCATAAAATATTTAAAGGGCCACAAATCCCCCACCGCACCACACCCCCCCCC
  CCCCCCCCCCCCCCCCAGCCCCCCCCCCCCCGCACACCCCCCCCCCCCCCCCACCCCAAA
  AACACCCCCCCTCTTAGCGGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014222 unedited
  CTTTTCATGGCCAGGTAAAGGTATAGNATAACTGCCAAGTCACATAAGTTAACTTTTTTG
  TTAATGGTTTGATGATCCCGGNAAATAACACACTATCAGTCGATGCAAACCGCATGGGCG
  TTTTCATCAGTCGTTGTCTGAGCACATGACCGAGTGTGGGCCACGGACCCATCTTTACTT
  GGTCCAGAAATAAAAGCGGCTGCCATGTGTGGCAGGCTGCAGATCTCCCTCGATCTCAGG
  GCTGGGATCCGGTCTTGGTCTTGAGTGATAGGGATTCTCCGGTAAAGGTCGATCTGTTTT
  CACTTTGGTGACCTTTGACAGTTCTCCCAGGTCAGGCCGCACCCATCCCAGTTTGTCCAG
  CACACACTCGTCAAACTTTGCCTGCTGTTTGCGACAGTGACGAAATAACTGCTGGCCAGT
  ATAATCAATGCAAGTCCAATATTCTGTAAAAGGCTCTGCACAGTGACGTTTTATCTGCCT
  AAAGAAGTCCAAAGCACACTTGTTGACCAGTTTGCCTTCCTCTAAACACCGCCTCGGATC
  TTTCTCTTCCCAGCGGCAGAGCATAAATTCCTTGTTGGGCTTATCACATTGAGCTCCATA
  GTGATGGGCCGCAGCTCTAAGCACAGCAGAACTAATTTTTACCCTCATCTACTTTTCAGC
  TCCTCTAGAGTGGGCAGCTCCACTATCCCCGGCATGACAGCTGCAGCCCCGATCCCGACG
  AGTATCCCTTATCCGCGTTGTCCCCCGCCTTGTGCCGTATTTCCCGGCCGCCCTATATGG
  AGTCGGTTTACAAAATTCTGACGGTTCACTAAACCAGCCTTGCTTATATAACCTTCCACC
  GTCACGCCTACCGCCTTTTGGCAAACGGGGCGAGTTTTACAACATTTTGAAAGTCCGCTG
  TTTTGGTGCCAAACAACTCCATGACGCCATGGGGGGAAACTTGTAATCCCGGGGGTAAAC
  GCTTCACCGCCTTGGGGGCCTGCCAACCCTTCCCATG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014222
• Insert Size: 870 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014222.2, NP_055037.1
• RefSeq Size: 859 bp
• RefSeq ORF: 519 bp
• Locus ID: 4702
• Cytogenetics: 9q33.2
• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease
• Gene Summary: 'The protein encoded by this gene belongs to the complex I 19 kDa subunit family. Mammalian complex I is composed of 45 different subunits. This protein has NADH dehydrogenase activity and oxidoreductase activity. It plays an important role in transfering electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]'
  Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (1).