SACM1L (NM_014016) Human Untagged Clone

CAT#: SC115176

SACM1L (untagged)-Human SAC1 suppressor of actin mutations 1-like (yeast) (SACM1L)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SACM1L
• Synonyms: SAC1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_014016, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGACGGCGGCCTACGAGCAGCTGAAGCTGCATATCACACCTGAAAAATTTTATGTGGAAGCTTGTG
  ATGATGGAGCAGATGACGTACTTACCATTGACCGTGTGTCCACAGAGGTTACCCTTGCAGTCAAGAAAGA
  TGTTCCTCCTTCAGCTGTCACAAGACCAATATTTGGTATACTGGGCACAATCCATCTGGTGGCAGGTAAT
  TATCTTATAGTCATTACCAAAAAGATAAAAGTAGGTGAATTTTTCAGTCATGTAGTCTGGAAAGCAACAG
  ATTTTGATGTCCTTTCTTATAAGAAGACAATGTTGCACTTAACTGATATTCAGTTACAAGATAATAAAAC
  CTTCCTAGCGATGCTAAACCATGTCTTGAATGTGGATGGATTTTACTTTTCAACAACATATGATTTGACC
  CATACTTTGCAGCGGCTATCCAACACTAGTCCTGAATTCCAAGAAATGAGTCTCTTGGAAAGGGCAGATC
  AGCGGTTTGTATGGAATGGTCATCTTCTAAGAGAACTTTCTGCACAGCCAGAGGTTCATCGGTTTGCCCT
  TCCAGTGTTACATGGCTTTATTACCATGCATTCATGTTCTATTAATGGAAAATACTTTGATTGGATTCTC
  ATCTCGAGGAGGAGCTGTTTCAGAGCTGGTGTGCGCTATTATGTAAGAGGAATTGATTCGGAAGGCCATG
  CAGCTAACTTTGTAGAAACAGAACAAATTGTGCACTACAATGGGAGCAAAGCTTCGTTTGTACAGACTCG
  AGGATCAATACCTGTTTTCTGGTCCCAAAGACCAAACCTCAAGTACAAACCACTGCCACAGATCAGCAAA
  GTAGCAAATCACATGGACGGTTTCCAAAGGCATTTTGATTCCCAAGTAATTATTTATGGAAAACAAGTTA
  TAATCAATCTGATTAACCAGAAGGGCTCGGAGAAGCCACTTGAGCAGACATTTGCAACAATGGTGTCTTC
  CTTGGGAAGTGGAATGATGAGATACATTGCCTTTGACTTCCATAAGGAATGTAAAAATATGAGATGGGAT
  CGACTAAGTATTTTATTGGATCAGGTAGCAGAAATGCAAGATGAATTAAGTTATTTTCTAGTGGACTCTG
  CTGGCCAGGTGGTGGCAAACCAGGAAGGCGTGTTCCGAAGCAATTGCATGGATTGTCTAGATAGAACCAA
  TGTGATCCAGAGTTTGTTAGCTCGTCGTTCACTTCAGGCCCAACTTCAGAGACTAGGAGTTTTGCATGTG
  GGACAAAAGCTTGAAGAACAAGATGAATTTGAGAAGATTTACAAAAATGCCTGGGCTGACAACGCAAATG
  CTTGTGCCAAGCAATATGCGGGAACTGGTGCCTTGAAGACTGACTTTACCAGAACTGGAAAGAGAACTCA
  TTTGGGACTTATAATGGATGGCTGGAACTCAATGATACGATATTATAAGAACAACTTTTCCGATGGATTT
  AGACAAGATTCCATAGACTTATTTCTTGGAAACTATTCAGTGGATGAATTAGAATCTCATAGTCCTTTAA
  GTGTTCCAAGGGACTGGAAATTCCTGGCTTTGCCTATTATCATGGTTGTTGCCTTTTCAATGTGCATTAT
  CTGTTTGCTTATGGCTGGTGACACTTGGACAGAAACACTGGCCTATGTGCTCTTCTGGGGAGTTGCAAGC
  ATTGGAACATTTTTTATCATTCTTTACAATGGCAAAGATTTTGTCGATGCTCCCAGACTGGTCCAGAAAG
  AAAAGATAGACTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014016 unedited
  CATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGAGGATGGCGACGGCG
  GCCTACGAGCAGCTGAAGCTGCATATCACACCTGAAAAATTTTATGTGGAAGCTTGTGAT
  GATGGAGCAGATGACGTACTTACCATTGACCGTGTGTCCACAGAGGTTACCCTTGCAGTC
  AAGAAAGATGTTCCTCCTTCAGCTGTCACAAGACCAATATTTGGTATACTGGGCACAATC
  CATCTGGTGGCAGGTAATTATCTTATAGTCATTACCAAAAAGATAAAAGTAGGTGAATTT
  TTCAGTCATGTAGTCTGGAAAGCAACAGATTTTGATGTCCTTTCTTATAAGAAGACAATG
  TTGCACTTAACTGATATTCAGTTACAAGATAATAAAACCTTCCTAGCGATGCTAAACCAT
  GTCTTGAATGTGGATGGATTTTACTTTTCAACAACATATGATTTGACCCATACTTTGCAG
  CGGCTATCCAACACTAGTCCTGAATTCCAAGAAATGAGTCTCTTGGAAAGGGCAGATCAG
  CGGTTTGTATGGAATGGTCATCTTCTAAGAGAACTTTCTGCACAGCCGGAGGTTCATCGG
  TTTGCCCTTCCAGTGTTACATGGCTTTATTACCATGCATTCATGTTCTATTAATGGAAAA
  TACTTTGATTGGATTCTCATCTCGAGGAGGAGCTGTTTCAGAGCTGGTGTGCGCTATTAT
  GTAAGAAGAATTGATTCGGAAGGCCATGCAGCTAACTTTGTAGAACAGAACAATTGTGCA
  CTCAATGGGGAGCAAGCTTCGTTTGTACAGACTCCGAGATCAATACCTGTTTTCCTGGTC
  CCAAAGACCAACCTCAGTACAAACCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014016
• ORF Size: 1764 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_014016.2, NP_054735.2
• RefSeq Size: 3550
• RefSeq ORF: 1764
• Locus ID: 22908
• Domains: Syja_N
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: This gene encodes an integral membrane protein, which is localized to the endoplasmic reticulum, and functions as a phosphoinositide phosphatase that hydrolyzes phosphatidylinositol 3-phosphate, phosphatidylinositol 4-phosphate, and phosphatidylinositol 3,5-bisphosphate. Deletion of this gene in mouse results in preimplantation lethality. Other studies suggest that this gene is also involved in the organization of golgi membranes and mitotic spindles. Alternatively spliced transcript variants have been found for this gene. A C-terminally extended isoform is also predicted to be produced by the use of an alternative in-frame, downstream translation termination codon via a stop codon readthrough mechanism. [provided by RefSeq, Dec 2017]
  Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon, while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the shorter isoform (1).