USP25 (NM_013396) Human Untagged Clone

CAT#: SC115248

USP25 (untagged)-Human ubiquitin specific peptidase 25 (USP25)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: USP25
• Synonyms: USP21
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115248 sequence for NM_013396 edited (data generated by NextGen Sequencing)
  ATGACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAAGCACCAGCAGACGTTT
  TTGAATCAACTGAGAGAAATTACGGGGATTAATGACACCCAGATACTACAGCAAGCCTTG
  AAGGATAGTAATGGAAACTTGGAATTAGCAGTGGCTTTCCTTACTGCGAAGAATGCTAAG
  ACCCCTCAGCAGGAGGAGACAACTTACTACCAAACAGCACTTCCTGGCAATGATAGATAC
  ATCAGTGTGGGAAGCCAAGCAGATACAAATGTGATTGATCTCACTGGAGATGATAAAGAT
  GATCTTCAGAGAGCAATTGCCTTGAGTTTGGCCGAATCAAACAGGGCATTCAGGGAGACT
  GGAATAACTGATGAGGAACAAGCCATTAGCAGAGTTCTTGAAGCCAGCATAGCAGAGAAT
  AAAGCATGTTTGAAGAGGACACCTACAGAAGTTTGGAGGGATTCTCGAAACCCTTATGAT
  AGAAAAAGACAGGACAAAGCTCCCGTTGGGCTAAAGAATGTTGGCAATACTTGTTGGTTT
  AGTGCTGTTATTCAGTCATTATTTAATCTTTTGGAATTTAGAAGATTAGTTCTGAATTAC
  AAGCCTCCATCAAATGCTCAAGATTTACCCCGAAACCAAAAGGAACATCGGAATTTGCCT
  TTTATGCGTGAGCTGAGGTATCTATTTGCACTTCTTGTTGGTACCAAAAGGAAGTATGTT
  GATCCATCAAGAGCAGTTGAAATTCTTAAGGATGCTTTCAAATCAAATGACTCACAGCAG
  CAAGATGTGAGTGAGTTTACACACAAATTATTAGATTGGTTAGAAGATGCCTTCCAAATG
  AAAGCTGAAGAGGAGACGGATGAAGAGAAGCCAAAGAACCCCATGGTAGAGTTGTTCTAT
  GGCAGATTCCTGGCTGTGGGAGTACTTGAAGGTAAAAAATTTGAAAACACTGAAATGTTT
  GGTCAGTACCCACTTCAGGTCAATGGGTTCAAAGATCTGCATGAGTGCCTAGAAGCTGCA
  ATGATTGAAGGAGAAATTGAGTCTTTACATTCAGAGAATTCAGGAAAATCAGGCCAAGAG
  CATTGGTTTACTGAATTACCACCTGTGTTAACATTTGAATTGTCAAGATTTGAATTTAAT
  CAGGCATTGGGAAGACCAGAAAAAATTCACAACAAATTAGAATTTCCCCAAGTTTTATAT
  TTGGACAGATACATGCACAGAAACAGAGAAATAACAAGAATTAAGAGGGAAGAGATCAAG
  AGACTGAAAGATTACCTCACGGTATTACAACAAAGGCTAGAAAGATATTTAAGCTATGGT
  TCCGGTCCCAAACGATTCCCCTTGGTAGATGTTCTTCAGTATGCATTGGAATTTGCCTCA
  AGTAAACCTGTTTGCACTTCTCCTGTTGACGATATTGACGCTAGTTCCCCACCTAGTGGT
  TCCATACCATCACAGACATTACCAAGCACAACAGAACAACAGGGAGCCCTATCTTCAGAA
  CTGCCAAGCACATCACCTTCATCAGTTGCTGCCATTTCATCGAGATCAGTAATACACAAA
  CCATTTACTCAGTCCCGGATACCTCCAGATTTGCCCATGCATCCGGCACCAAGGCACATA
  ACGGAGGAAGAACTTTCTGTGCTGGAAAGTTGTTTACATCGCTGGAGGACAGAAATAGAA
  AATGACACCAGAGATTTGCAGGAAAGCATATCCAGAATCCATCGAACAATTGAATTAATG
  TACTCTGACAAATCTATGATACAAGTTCCTTATCGATTACATGCCGTTTTAGTTCACGAA
  GGCCAAGCTAATGCTGGGCACTACTGGGCATATATTTTTGATCATCGTGAAAGCAGATGG
  ATGAAGTACAATGATATTGCTGTGACAAAATCATCATGGGAAGAGCTAGTGAGGGACTCT
  TTTGGTGGTTATAGAAATGCCAGTGCATACTGTTTAATGTACATAAATGATAAGGCACAG
  TTCCTAATACAAGAGGAGTTTAATAAAGAAACTGGGCAGCCCCTTGTTGGTATAGAAACA
  TTACCACCGGATTTGAGAGATTTTGTTGAGGAAGACAACCAACGATTTGAAAAAGAACTA
  GAAGAATGGGATGCACAACTTGCCCAGAAAGCTTTGCAGGAAAAGCTTTTAGCGTCTCAG
  AAATTGAGAGAGTCAGAGACTTCTGTGACAACAGCACAAGCAGCAGGAGACCCAGAATAT
  CTAGAGCAGCCATCAAGAAGTGATTTCTCAAAGCACTTGAAAGAAGAAACTATTCAAATA
  ATTACCAAGGCATCACATGAGCATGAAGATAAAAGTCCTGAAACAGTTTTGCAGTCGGCA
  ATTAAGTTGGAATATGCAAGGTTGGTTAAGTTGGCCCAAGAAGACACCCCACCAGAAACC
  GATTATCGTTTACATCATGTAGTGGTCTACTTTATCCAGAACCAGGCACCAAAGAAAATT
  ATTGAGAAAACATTACTAGAACAATTTGGAGATAGAAATTTGAGTTTTGATGAAAGGTGT
  CACAACATAATGAAAGTTGCTCAAGCCAAACTGGAAATGATAAAACCTGAAGAAGTAAAC
  TTGGAGGAATATGAGGAGTGGCATCAGGATTATAGGAAATTCAGGGAAACAACTATGTAT
  CTCATAATTGGGCTAGAAAATTTTCAAAGAGAAAGTTATATAGATTCCTTGCTGTTCCTC
  ATCTGTGCTTATCAGAATAACAAAGAACTCTTGTCTAAAGGCTTATACAGAGGACATGAT
  GAAGAATTGATATCACATTATAGAAGAGAATGTTTGCTAAAATTAAATGAGCAAGCCGCA
  GAACTCTTCGAATCTGGAGAGGATCGAGAAGTAAACAATGGTTTGATTATCATGAATGAG
  TTTATTGTCCCATTTTTGCCATTATTACTGGTGGATGAAATGGAAGAAAAGGATATACTA
  GCTGTAGAAGATATGAGAAATCGATGGTGTTCCTACCTTGGTCAAGAAATGGAACCACAC
  CTCCAAGAAAAGCTGACAGATTTTTTGCCAAAACTGCTTGATTGTTCTATGGAGATTAAA
  AGTTTCCATGAGCCACCGAAGTTACCTTCATATTCCACGCATGAACTCTGTGAGCGATTT
  GCCCGAATCATGTTGTCCCTCAGTCGAACTCCTGCTGATGGAAGATAA
  
  Clone variation with respect to NM_013396.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013396 unedited
  AGGTCAGAAATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGAGCCCG
  CGGACCGGCAGCAAAGGACGTGCGAACGCGTGACGCCGCCCGACTGGCTCGCGCTCTCCC
  GTGCCCCGGCGTCCTCCGCCCGCTCATGGCCCGGGCCGCCGCGGACGAGCGGCGCTGAGG
  CGGGCCGCGTGGAGACGTGAGGCGGCCGCCGTGGCCCTCACAGTCGGCGTTTCGCCGCCT
  GCCCGCGGTGCCCGCGCACGCCGGCCGCCATCGCCTTCGCGCCTGGCTGGCGGGGGCGCT
  GTCCTCCCAGGCCGTCCGCGCCGCTCCCTGGAGCTCGGCGGAGCGCGGCAGCCAGGGCCG
  GCGGAGGCGCGAGGAGCCGGGCGCCACCGCCGCCGCCGCCGCCGCCGCCGCGGGGGCCAT
  GACCGTGGAGCAGAACGTGCTGCAGCAGAGCGCGGCGCAGAAGCACCAGCAGACGTTTTT
  GAATCAACTGAGAGAAATTACGGGGATTAATGACACCCAGATACTACAGCAAGCCTTGAA
  GGATAGTAATGGAAACTTGGAATTAGCAGTGGCTTTCCTTACTGCGAAGAATGCTAAGAC
  CCCTCAGCAGGAGGAGACAACTTACTACCAAACAGCACTTCCTGGCAATGATAGATACAT
  CAGTGTGGGAAGCCAAGCAGATACAAATGTGATTGATCTCACTGGAGATGATAAAGATGA
  TCCTCAGAGAGCAATTGCCTTGAGTTTGGCCCGATCAAACAGGGCATTCAGGCAGACTGG
  AATAACTGATGACGAACAAGCCCTTACCAGAGTTCTTTGAACCAGCATACCAGAGAATAA
  GCCTGTTTTGAAGAAGACCCTACAGAAGTTGTGGAGGGATTCTCCAAACCCTTATGATAG
  A
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013396 unedited
  ATGGACCGCGGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTAGCCATAAATAA
  AACTTTATTCTTTATTTCATTTACAAGCTACCAAATATTATGTATCGTACACAGTGCTGA
  ACACTTAAATGGCTGTAGTCATGGAAGGATCCAGACTGAATGGAAAGCTGTTGAGAAAGA
  AAAGATAAAAGCAAAGTAATACTGCAACAGGAAGGTGGCAAAAGCATAGTTTTGCCATAA
  TAAAATCAATTAGATTTGTGATTATACATCAGTTCCGGTTAAAATGTCTGAGCGCCATGC
  GATTTTCAGCTTTATTGTCTGCAGTCTGACTAAAGTCTGTATAGTCATTTTGTCTTTTGC
  AGTTATTAAAATAAAAAAAAGTTAAAAACTATAGCAGCAACAAGCAAACCCTGTGACAGG
  AAGGCAAGGGTTAAGAACTAAAAAGAGTTTATACAGTGTGTTCAGGGAAAGTGTGCAGTT
  TATCTTCCATCAGCAGGAGTTCGACTGAGGGACAACATGATTCGGGCAAATCGCTCACAG
  AGTTCATGCGTGGAATATGAAGGTAACTTCGGTGGCTCATGGAAACTTTTATCTCCTAGA
  ACAATCAAGCAGTTTTGGCAAAAAATCTGTCCACTTTTTTTGGAAGTGTGGTTCCCTTTC
  TTGACCAAGGTAGGAACCCCATCGGATTCTCATATTTTTTACAGCTAAGATATCCTCTTT
  TTCCTTTTCATCCACCAGTATTAATGGCAAAAATGGGACAATTAACTTTATTCTTGATAA
  TTAACCCTTTGTTTCCTTCTTGAACCCCCCCAAATCCAAAATTTTGGCGTTTGCCCTTTA
  ATTTACAAAAATTTCTTTTTTATGCGACAAAATTCTTATCATTCCCCTGTTTACACCTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_013396
• ORF Size: 3168 bp
• Insert Size: 4500
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_013396.3, NP_037528.3
• RefSeq Size: 4968
• RefSeq ORF: 3168
• Locus ID: 29761
• Domains: UCH, UIM
• Protein Families: Protease
• Gene Summary: Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25 (Valero et al., 1999 [PubMed 10644437]). [supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (3) lacks two alternate in-frame exons in the 3' coding region, compared to variant 1, resulting in an isoform (USP25a) that is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.