CNOT4 (NM_013316) Human Untagged Clone

CAT#: SC115278

CNOT4 (untagged)-Human CCR4-NOT transcription complex, subunit 4 (CNOT4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CNOT4
• Synonyms: CLONE243; NOT4; NOT4H
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_013316, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCTCGCAGTCCTGATGCGAAGGAAGACCCTGTGGAGTGCCCTCTTTGCATGGAGCCCTTGGAGATAG
  ATGATATCAACTTTTTCCCTTGCACCTGTGGCTACCAGATTTGCCGATTTTGTTGGCATCGAATTCGCAC
  TGATGAAAATGGGCTTTGTCCTGCATGTAGAAAGCCATATCCAGAAGACCCAGCAGTTTATAAACCACTC
  TCCCAGGAAGAGCTGCAAAGGATAAAGAATGAGAAAAAACAGAAACAAAATGAGAGAAAACAGAAAATAT
  CAGAAAATCGCAAACATTTGGCTAGTGTACGTGTCGTACAAAAAAACCTCGTCTTTGTTGTAGGTTTATC
  TCAGCGCCTAGCAGACCCAGAGGTTTTAAAACGACCAGAATATTTTGGGAAGTTTGGTAAAATACATAAA
  GTTGTCATCAATAATAGCACATCATATGCAGGCTCACAGGGTCCAAGTGCCAGTGCTTATGTAACCTATA
  TCCGGTCAGAAGACGCTCTCAGAGCCATACAGTGTGTCAACAATGTGGTAGTAGATGGCAGAACACTTAA
  GGCATCTCTAGGTACAACAAAATACTGCAGTTACTTCTTAAAGAATATGCAGTGTCCAAAACCTGACTGC
  ATGTATCTTCATGAATTGGGGGATGAGGCGGCCAGCTTCACAAAAGAGGAAATGCAGGCGGGTAAACACC
  AAGAATATGAACAGAAGCTACTTCAAGAATTATATAAATTAAATCCCAATTTTCTTCAGCTATCTACGGG
  TTCAGTTGATAAAAATAAGAACAAAGTGACACCACTGCAGAGCCCCATTGACAAACCTTCAGATTCTCTC
  AGTATAGGGAACGGTGATAATTCCCAGCAGATATCTAACAGTGATACGCCTTCACCACCACCTGGTTTGT
  CAAAATCCAATCCAGTCATCCCCATCAGTTCATCCAATCACAGTGCACGGTCCCCTTTTGAAGGGGCAGT
  AACAGAGTCACAGTCGTTATTCTCAGACAATTTTCGCCATCCCAACCCTATCCCAAGTGGGCTTCCTCCT
  TTCCCCAGCTCCCCACAGACATCCAGTGACTGGCCTACAGCACCAGAACCACAGAGCCTCTTCACATCAG
  AAACAATCCCAGTATCATCCTCTACAGACTGGCAAGCAGCTTTTGGCTTTGGTTCTTCTAAACAACCAGA
  GGATGACTTGGGTTTTGATCCCTTCGATGTCACTCGAAAAGCCTTAGCAGACCTGATTGAGAAGGAACTG
  TCCGTTCAAGACCAACCTTCCCTTTCGCCCACATCTCTTCAGAACTCCTCTTCACACACTACAACCGCCA
  AAGGTCCAGGCTCTGGATTCCTGCATCCTGCTGCAGCTACAAATGCCAATTCTCTCAATAGTACCTTTTC
  AGTCTTGCCCCAGAGGTTCCCTCAATTTCAGCAGCACCGAGCGGTTTATAATTCATTCAGTTTTCCAGGC
  CAGGCAGCCCGCTATCCTTGGATGGCCTTTCCACGCAATAGCATCATGCACTTGAACCACACAGCAAACC
  CCACCTCAAATAGTAATTTCTTGGACTTGAATCTCCCGCCACAGCACAACACAGGTCTGGGAGGGATCCC
  TGTAGCAGGTATTCCAGCGTCTTCAGGAAACAGTTTAGACTCTCTTCAAGATGACAATCCTCCACACTGG
  CTAAAATCCCTTCAGGCCCTCACAGAGATGGACGGCCCCAGCGCTGCTCCATCACAGACCCACCACAGCG
  CCCCCTTCAGCACACAGATCCCGCTGCACAGAGCCAGTTGGAATCCCTACCCTCCTCCTTCAAACCCTTC
  CAGCTTCCACTCCCCACCCCCAGGCTTTCAGACAGCCTTCAGACCCCCCAGCAAAACCCCCACAGATTTA
  CTACAGAGTTCAACACTGGACCGCCATTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_013316 unedited
  TTCGGGATTTGTATACGACTCACTATAGGCGGCCGCGAATCGGCACGAGGGAAGAGTTTT
  TTCCCCGACGCTCGTTCGGTGGTTTCTTGTGAGGACGCGGCAGCCCGTGGAGTCGGCTGA
  GGGAAAAGCGAGGCAAGGCAGGCGGATCCTTTCAATATACACTTCCAGAAAAAGGGAAGC
  CCCGTGGTGGCCCTTGAAAAGTAAAAGGAAGTTGGGAAGTGAATGAAGTGAGGTCGTCAA
  AGTCCTACATTTTAGCTTTCAGTGCTGAACTTCCTTCTCCCTTAAATTATTATAAACTTT
  TGCTGCTGTTTAATAAACGTGAAGATGTCTCGCAGTCCTGATGCGAAGGAAGACCCTGTG
  GAGTGCCCTCTTTGCATGGAGCCCTTGGAGATAGATGATATCAACTTTTTCCCTTGCACC
  TGTGGCTACCAGATTTGCCGATTTTGTTGGCATCGAATTCGCACTGATGAAAATGGGCTT
  TGTCCTGCATGTAGAAAGCCATATCCAGAAGACCCAGCAGTTTATAAACCACTCTCCCAG
  GAAGAGCTGCAAAGGATAAAGAATGAGAAAAAACAGAAACAAAATGAGAGAAAACAGAAA
  ATATCAGAAAATCGCAAACATTTGGCTAGTGTACGTGTCGTACAAAAAAACCTCGTCTTT
  GTTGTAGGTTTATCTCAGCGCCTAGCAGACCCAGAGGTTTTAAAACGACCAGAATATTTT
  GGGAAAGTTGGTAAATACATAAAGTTGTCATCAATAATAGCACATCATATGCAGGCTCAC
  AGGGTCCAAGTGCCAGTGCTTATGTAACCTATATCCGGTCAGAAGACGCTCTCAGACCAT
  ACAGTGTGTCAACATGTGGTAGTAGATGCCAGAACACTTAGGCATCTCTAGGTACACAAA
  TACTGCAGTACTTCTTAAGAAAAGCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_013316 unedited
  CCGCAATTTAGAGTCGAGTTTTTTTTTTTTTTTTTTATAATCAAGTTTTCTGTTTTATTT
  AAGATTTTAGTTTTAATTCTGAAGAAAGAGTACATTTCCTTGTTTTTAGTTTTAGCGTCA
  GGTTATAACCAGGTTTCTGTTTTCTTTTCTCCTGTCTTTTCAATACTGCACAAACGTCCA
  GAAACTACAGGGTTAAGTAAAAGCTGCAGGCAAGGAGGTCAGAGATTTGCTCCCGATGGT
  GATCAGATGGCCCCAGAGCAGTGCTATCTCAGAGTGCCCTCGTCAGGTGAATTTATGGTA
  AAACAAAAAAGATTCCGACAGAGGAGAAGGACTGAAGAAATGACTTACATTTCAGCTCTT
  ATATTATTAATAGGTATTAAATCTGTGAATACTTTTTTCTTTAAAACATTTCAATTTACT
  TTAAATCTAAAGCCAAAAAATTTTTCTCCTTTAAAAAAATTACTTCTCACCTCCTTATGA
  ACTCTTTAGCAATCGGCCAGTTACTACTACAGATGAATTTGTCTTTCCATCAACTTCCAA
  TACATTCTTTTGCTTAATGACACAATCAAGTCCTTGCCATCAACTTGCCAAAAGAATTAA
  AAATTAATTTAAATTAAAAGCATTTTCCCTGCAATAATGATAAGCTGAATACAAGATGGA
  TGCTAGGACCACTGCTTTCCCCCATGCATTTAGCAATCTCACGTAAATGAACCTGGTTTT
  CAAACTTCTGCAGTTGATAATGCATTTACTTGAGCTTTCCTATAGATTAATCGTCATTTT
  CTGCTAAGCAGANATGTCATTATTATGCGCAACAGACAAACATAATTTCTAAGTATGAGA
  CTGCCCTTGATTTTTTTTCTCCTCTTAAAAGCATTTTAGAACATTCACATGTCACTCAAG
  CTGGACACAAAATTTTACATTATAAGAGATGTATGACCTGGATCCATGCATCGGGTAGGA
  AN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_013316
• Insert Size: 3370 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013316.2, NP_037448.2
• RefSeq Size: 3333 bp
• RefSeq ORF: 1920 bp
• Locus ID: 4850
• Cytogenetics: 7q33
• Domains: RRM, RRM_1
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: RNA degradation
• Gene Summary: 'The protein encoded by this gene is a subunit of the CCR4-NOT complex, a global transcriptional regulator. The encoded protein interacts with CNOT1 and has E3 ubiquitin ligase activity. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2010]'
  Transcript Variant: This variant (1) uses an alternate in-frame splice junction at the 3' end of an exon and lacks an alternate in-frame exon compared to variant 6. The resulting isoform (a) has the same N- and C-termini but is shorter compared to isoform f. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.