PI 3 Kinase p85 alpha (PIK3R1) (NM_181523) Human Untagged Clone

CAT#: SC115320

PIK3R1 (untagged)-Human phosphoinositide-3-kinase, regulatory subunit 1 (alpha) (PIK3R1), transcript variant 1


  "NM_181523" in other vectors (6)

Reconstitution Protocol

USD 760.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PIK3R1
Synonyms AGM7; GRB1; IMD36; p85; p85-ALPHA
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_181523 edited
ATGAGTGCTGAGGGGTACCAGTACAGAGCGCTGTATGATTATAAAAAGGAAAGAGAAGAA
GATATTGACTTGCACTTGGGTGACATATTGACTGTGAATAAAGGGTCCTTAGTAGCTCTT
GGATTCAGTGATGGACAGGAAGCCAGGCCTGAAGAAATTGGCTGGTTAAATGGCTATAAT
GAAACCACAGGGGAAAGGGGGGACTTTCCGGGAACTTATGTAGAATATATTGGAAGGAAA
AAAATCTCGCCTCCCACACCAAAGCCCCGGCCACCTCGGCCTCTTCCTGTTGCACCAGGT
TCTTCGAAAACTGAAGCAGATGTTGAACAACAAGCTTTGACTCTCCCGGATCTTGCAGAG
CAGTTTGCCCCTCCTGACATTGCCCCGCCTCTTCTTATCAAGCTCGTGGAAGCCATTGAA
AAGAAAGGTCTGGAATGTTCAACTCTATACAGAACACAGAGCTCCAGCAACCTGGCAGAA
TTACGACAGCTTCTTGATTGTGATACACCCTCCGTGGACTTGGAAATGATCGATGTGCAC
GTTTTGGCTGACGCTTTCAAACGCTATCTCCTGGACTTACCAAATCCTGTCATTCCAGCA
GCCGTTTACAGTGAAATGATTTCTTTAGCTCCAGAAGTACAAAGCTCCGAAGAATATATT
CAGCTATTGAAGAAGCTTATTAGGTCGCCTAGCATACCTCATCAGTATTGGCTTACGCTT
CAGTATTTGTTAAAACATTTCTTCAAGCTCTCTCAAACCTCCAGCAAAAATCTGTTGAAT
GCAAGAGTACTCTCTGAAATTTTCAGCCCTATGCTTTTCAGATTCTCAGCAGCCAGCTCT
GATAATACTGAAAACCTCATAAAAGTTATAGAAATTTTAATCTCAACTGAATGGAATGAA
CGACAGCCTGCACCAGCACTGCCTCCTAAACCACCAAAACCTACTACTGTAGCCAACAAC
GGTATGAATAACAATATGTCCTTACAAGATGCTGAATGGTACTGGGGAGATATCTCGAGG
GAAGAAGTGAATGAAAAACTTCGAGATACAGCAGACGGGACCTTTTTGGTACGAGATGCG
TCTACTAAAATGCATGGTGATTATACTCTTACACTAAGGAAAGGGGGAAATAACAAATTA
ATCAAAATATTTCATCGAGATGGGAAATATGGCTTCTCTGACCCATTAACCTTCAGTTCT
GTGGTTGAATTAATAAACCACTACCGGAATGAATCTCTAGCTCAGTATAATCCCAAATTG
GATGTGAAATTACTTTATCCAGTATCCAAATACCAACAGGATCAAGTTGTCAAAGAAGAT
AATATTGAAGCTGTAGGGAAAAAATTACATGAATATAACACTCAGTTTCAAGAAAAAAGT
CGAGAATATGATAGATTATATGAAGAATATACCCGCACATCCCAGGAAATCCAAATGAAA
AGGACAGCTATTGAAGCATTTAATGAAACCATAAAAATATTTGAAGAACAGTGCCAGACC
CAAGAGCGGTACAGCAAAGAATACATAGAAAAGTTTAAACGTGAAGGCAATGAGAAAGAA
ATACAAAGGATTATGCATAATTATGATAAGTTGAAGTCTCGAATCAGTGAAATTATTGAC
AGTAGAAGAAGATTGGAAGAAGACTTGAAGAAGCAGGCAGCTGAGTATCGAGAAATTGAC
AAACGTATGAACAGCATTAAACCAGACCTTATCCAGCTGAGAAAGACGAGAGACCAATAC
TTGATGTGGTTGACTCAAAAAGGTGTTCGGCAAAAGAAGTTGAACGAGTGGTTGGGCAAT
GAAAACACTGAAGACCAATATTCACTGGTGGAAGATGATGAAGATTTGCCCCATCATGAT
GAGAAGACATGGAATGTTGGAAGCAGCAACCGAAACAAAGCTGAAAACCTGTTGCGAGGG
AAGCGAGATGGCACTTTTCTTGTCCGGGAGAGCAGTAAACAGGGCTGCTATGCCTGCTCT
GTAGTGGTGGACGGCGAAGTAAAGCATTGTGTCATAAACAAAACAGCAACTGGCTATGGC
TTTGCCGAGCCCTATAACTTGTACAGCTCTCTGAAAGAACTGGTGCTACATTACCAACAC
ACCTCCCTTGTGCAGCACAACGACTCCCTCAATGTCACACTAGCCTACCCAGTATATGCA
CAGCAGAGGCGATGA
>OriGene 5' read for NM_181523 unedited
AGTATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGGCGGAGTTG
GAGGAAGCAGCGGCAGCGGCGAGGGCGGCAGGCTAGCTGTCGGAGACGGCAAGCACGGAT
CGGGCACGGCCGGCGGGTCGCAGCCGGGCTGGAGGCCGGTCGGAACCGAGCGGGCAGGAG
GCCACCGCTGCAGCGCGGGCCCCGCAGAGGAAGGAAGCCGGCGGGCGGGGTAGGTGAAGC
TCGTGTGTGGAGTGCCACGGTACAATCAGACGACAGATGGACAGTGTGACAAAAGTGTCA
GAAAGGATTGGGCCTCGCTGTGAGAGTCAGCCTGGATTCAAAGTGTTGACAAGTTGCTGA
AAAGGAAGCCAGTGAGAGGACTGTGGCACGCAGAGGAAGTGGAGCCCTGTCTTCGGTCAC
ACCATTGATGGAGGACAGATGGACAGCCGTATGGCCAGTCACCTCTCCTCTTAAACCTTT
GGAGAGTGGTCCTTTGTCCTCTGCTGGACACATAATAGGAATTCTAACACATTCTCTGAA
TTCACTTTTCATAAAAACGTAAAATCAGACTGCTCTGTACAACCAGGCTCAACTGTTGCA
TGGTAGCAGATTTGCAAACATGAGTGCTGAGGGGTACCAGTACAGAGCGCTGTATGATTA
TAAAAAGGAAAGAGAAGAAGATATTGACTTGCACTTGGGTGACATATTGACTGTGAATAA
AGGGTCCTTAGTAGCTCTTGGATTCAGTGATGGACAGGAAGCCCAGCCTGTAGAANATGG
CTGGTTAAATGGCTATAATGAAACCACAGGGGAAAGGGGGGACTNTCCCGGGACTTATGT
AGGATATATTGGAAGGAAAAAAATCTCGCCTTCCACACCAAAGCCCCGGCCACCTCGGCT
CTTNNCTGTGCACCAGTTCTTCGAAACTGAAGCAGATGTTGACAACAGCTTTGN
>OriGene 3' read for NM_181523 unedited
NNNTTTTACTTTGAACCCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTAA
ATATTTCATAGTTTTAATTCTGAAGCCATTTTTTTTTTTTACTGGCATCCTGTACATTTA
CTTTTAAAAAAGGATAACAAAAATGAATATTAACAAAAATCCGGGACAACAATATTTTCA
AGCAACAAAAACTGGGGTGGGGAAGCTTATTCTGAAGGTACATTTAAAACTGAAATAACA
ACTTAATGAAAATTAAAAATTGCATAGCGCTGTGAATTTAGCCTTCAGCAAAACAAAACA
GAAGCTATTTGGTATTGATACAAATCCATCTATTTGATAGTTAGTCATCCAATATTATGT
ACATATTTTATATACTGAATGTCATTTTAAGTCCTGTTTTCCAAACTCCATTTTTCTGTT
GCTGGGTTTTTGTTTTTTGACAAGTTAAACACTTTCTGGCACTTTCTATGACAGAATTTC
TTCTGAACATACATGAACTGACATTCTCCCAAAGCGTCCCTTGTGAGTGGACGTGCCTTT
CTGCTACATATCGTTCATTTGTTACAAAATGAAATAATCCACAGTGCGATGTGTCTGGGT
CCACCGTGCACAGCAACATCCAGGCTAAACCAGGCTGGACCAAACCTTCTCAGGCCAGGG
TTCTTNTGCCTCTCTCTCCTCCGCTCTCCAGAGTCCCAACTTTCAGCTGGTAAAGCTTCA
GAAAGCATTAACAAGCACCATGGTTAAAGGCCTCTTTGTCCCTGCCGCAGAGATTTTGCA
TTTCTTTTCTCTTTGNGTGNATGGGGGNTGGGGCTTTAAATAAACCAAAGAAAAAGAAAA
GAAGGATAAGCACTCACTTAGAACGTGTGTCATCTACACCTTAGCTGAATGGCTTCCCTA
CTTT
Restriction Sites NotI-NotI     
ACCN NM_181523
Insert Size 3890 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_181523.1, NP_852664.1
RefSeq Size 6453 bp
RefSeq ORF 2175 bp
Locus ID 5295
Cytogenetics 5q13.1
Protein Families Druggable Genome
Protein Pathways Acute myeloid leukemia, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Insulin signaling pathway, Jak-STAT signaling pathway, Leukocyte transendothelial migration, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Phosphatidylinositol signaling system, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, VEGF signaling pathway
Gene Summary 'Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]'
Transcript Variant: This variant (1) encodes the longest isoform (1) of this protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.