ATP6V0A2 (NM_012463) Human Untagged Clone

CAT#: SC115366

ATP6V0A2 (untagged)-Human ATPase, H+ transporting, lysosomal V0 subunit a2 (ATP6V0A2)


  "NM_012463" in other vectors (6)

Reconstitution Protocol

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ATP6V0A2
Synonyms A2; ARCL; ARCL2A; ATP6A2; ATP6N1D; J6B7; RTF; STV1; TJ6; TJ6M; TJ6S; VPH1; WSS
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC115366 sequence for NM_012463 edited (data generated by NextGen Sequencing)
ATGGGGTCCCTGTTCCGGAGCGAGACCATGTGCCTGGCGCAGCTCTTCCTGCAGTCGGGC
ACGGCCTACGAGTGCCTCAGCGCCCTGGGCGAGAAAGGCCTGGTCCAGTTCCGAGACCTC
AACCAGAACGTAAGTTCTTTCCAAAGAAAATTTGTTGGTGAGGTGAAGAGGTGTGAAGAG
CTAGAGCGAATATTGGTGTATTTGGTACAGGAAATTAATAGAGCTGATATTCCCCTTCCT
GAAGGAGAGGCCAGCCCTCCTGCGCCACCCCTGAAACAGGTTCTAGAAATGCAGGAGCAG
TTGCAGAAGCTCGAGGTTGAACTGAGAGAAGTCACTAAGAACAAGGAGAAACTGAGGAAA
AACTTGCTGGAACTGATAGAGTACACTCACATGCTGAGAGTGACAAAGACCTTTGTGAAA
CGCAACGTTGAGTTTGAACCCACTTATGAAGAATTCCCTTCCTTAGAGAGCGATTCTTTG
TTGGATTACAGCTGTATGCAGAGGCTGGGAGCAAAACTGGGATTTGTGTCTGGCCTAATT
AACCAAGGAAAAGTGGAAGCATTTGAAAAAATGTTGTGGAGAGTCTGCAAAGGGTACACC
ATCGTGTCCTATGCAGAACTGGATGAATCCCTTGAAGACCCTGAAACAGGGGAAGTCATA
AAATGGTATGTCTTTTTAATATCCTTTTGGGGAGAGCAGATTGGCCACAAGGTTAAGAAG
ATATGTGATTGCTACCACTGCCACGTGTACCCCTATCCAAACACAGCCGAGGAGCGGAGG
GAGATCCAGGAGGGGCTGAACACCCGCATCCAGGATCTCTACACTGTACTGCACAAAACC
GAGGACTATTTGAGGCAAGTGCTATGTAAAGCCGCCGAGTCTGTCTACAGCCGTGTGATC
CAGGTGAAGAAAATGAAGGCCATCTATCACATGCTGAACATGTGCAGCTTTGACGTGACC
AACAAGTGCCTCATTGCTGAGGTCTGGTGTCCCGAGGCGGATCTGCAGGACCTGCGCCGG
GCACTGGAGGAGGGCTCGAGAGAGAGTGGTGCTACAATCCCCTCATTCATGAATATAATC
CCCACAAAAGAAACACCCCCCACTCGGATCCGCACCAACAAATTCACCGAGGGATTTCAG
AACATCGTGGATGCTTATGGAGTCGGAAGCTACAGAGAAGTCAATCCAGCTCTCTTTACC
ATCATCACCTTCCCGTTTTTATTTGCTGTGATGTTTGGAGACTTCGGACATGGCTTTGTG
ATGTTTTTATTTGCCCTCTTGTTGGTGTTAAATGAAAATCATCCCAGACTAAATCAGTCA
CAAGAGATCATGAGGATGTTTTTTAATGGCCGGTACATCCTCCTGCTGATGGGGCTGTTC
TCAGTGTACACTGGCCTCATCTACAACGACTGCTTTTCAAAGTCAGTCAACCTGTTCGGC
TCTGGGTGGAACGTGTCGGCCATGTACAGCTCCAGCCACCCACCCGCAGAGCATAAGAAG
ATGGTGCTTTGGAACGACAGCGTCGTTAGACACAACAGCATTTTGCAGCTGGATCCAAGC
ATTCCTGGAGTGTTCCGAGGCCCTTATCCCCTTGGCATTGATCCTATTTGGAACTTGGCC
ACAAATCGCCTCACTTTTCTAAACTCTTTCAAAATGAAAATGTCCGTGATTTTAGGAATC
ATTCATATGACTTTTGGAGTCATTCTGGGAATATTTAACCACTTGCACTTCAGGAAGAAG
TTCAACATTTACCTGGTTTCCATCCCGGAACTTCTCTTCATGCTCTGTATCTTTGGATAC
CTTATATTTATGATTTTCTACAAGTGGCTGGTTTTTTCAGCAGAAACCTCCAGAGTTGCT
CCCAGCATTCTGATTGAATTTATTAACATGTTTTTATTCCCAGCCAGTAAAACAAGTGGC
CTTTACACAGGGCAGGAGTATGTCCAGAGAGTGCTGCTGGTTGTCACAGCATTGTCTGTC
CCTGTCCTCTTCTTGGGAAAGCCACTGTTTTTGTTGTGGCTTCACAATGGGCGTAGTTGC
TTCGGGGTGAACCGGAGTGGCTACACACTTATAAGGAAAGATAGTGAGGAAGAAGTTTCA
TTGCTGGGAAGCCAAGATATAGAAGAGGGAAATCACCAGGTGGAAGATGGATGTAGAGAA
ATGGCGTGTGAAGAGTTTAATTTTGGAGAAATATTAATGACCCAAGTAATCCATTCCATC
GAGTACTGTCTGGGATGCATCTCCAACACCGCCTCCTACCTGAGGCTCTGGGCGCTTAGC
CTGGCTCACGCACAGTTGTCTGATGTCCTGTGGGCCATGCTGATGCGCGTGGGCCTCCGC
GTTGACACCACCTATGGCGTCTTGCTACTGCTCCCGGTTATCGCGCTCTTTGCAGTTTTG
ACCATTTTCATCCTTCTGATCATGGAAGGGCTTTCTGCGTTTCTTCACGCCATACGCCTC
CACTGGGTAGAATTTCAGAACAAATTCTACGTTGGTGCAGGCACCAAATTTGTTCCTTTC
TCATTCAGTCTACTTTCATCAAAGTTCAATAACGACGACAGTGTGGCATGA

Clone variation with respect to NM_012463.3
426 t=>c;471 t=>c;1515 t=>c
>OriGene 5' read for NM_012463 unedited
TCAAATTTTGTAATACGACTCACTATTAGGGCGGCCGCGATTCGGCACGAGGCGGACTGC
TGTGGCGGCAGCTGGAGCGGCGGCCGCGGTGGCAGAACCGGGGGCGGCCGCTGCAGTCTG
GAGCCCCATAGTGCGGGGCCGCGGCCAGGCCACAGGAAGAGCTCGAGGCCCGGGCCGCAC
CGGCTGAGTGTGCGGGCCCGCGCGGCTCGGAGCCGCCGCCGCCCATCGAGCCCCTCCGGG
CGCGGGTCGGCCCGCCATGGGGTCCCTGTTCCGGAGCGAGACCATGTGCCTGGCGCAGCT
CTTCCTGCAGTCGGGCACGGCCTACGAGTGCCTCAGCGCCCTGGGCGAGAAAGGCCTGGT
CCAGTTCCGAGACCTCAACCAGAACGTAAGTTCTTTCCAAAGAAAATTTGTTGGTGAGGT
GAAGAGGTGTGAAGAGCTAGAGCGAATATTGGTGTATTTGGTACAGGAAATTAATAGAGC
TGATATTCCCCTTCCTGAAGGAGAGGCCAGCCCTCCTGCGCCACCCCTGAAACAGGTTCT
AGAAATGCAGGAGCAGTTGCAGAAGCTCGAGGTTGAACTGAGAGAAGTCACTAAGAACAA
GGAGAAACTGAGGAAAAACTTGCTGGAACTGATAGAGTACACTCACATGCTGAGAGTGAC
AAAGACCTTTGTGAAACGCAACGTTGAGTTTGAACCCACTTATGAAGAATTCCCTTCCTT
AGAGAGCGATTCTTTGTTGGATTACAGCTGTATGCAGNAGCTGGGAGCAAAACTGGATTT
GTGTCTGGCCTAATAACCAAGGAAAGTGGAGCATTGAAAAATGTTGTGGAGAGTCTNGCA
AGGTACACCATCGTGTCTATGCAAACTGGATGATCCCTTGAGACCTGAACAGGGGAGTCA
TAAGGNATGTCTTTTAATATCCTTTGGGAGACAGATGGCCACAGGTAGAAGATTGTGATG
CTAC
>OriGene 3' read for NM_012463 unedited
NNTTCGGTACACCTAAGNNACCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTT
TTTTTTTTTTTCATGGAAAAATATCTTCATTTATATTTTGTTACAAAAATATACAAAATA
CTGCTTTTCACCTTTCCTTCACTCAGTATAAACCCTTCTGGTTCAGTGACATCAGCCAAG
CTGTCACTAAATTCAAATCATGTCCGCATTATATCTTCTACAGTCACACTGGGTTTTAAG
GCTCAGCATACAATTTATCTTCCAAACATACAATTATCATAACTGGTTTTATGTAAGGGT
AACAGGATAAGAGTCTTTAAAAAAATATACAAGTGGGATAACCATTTGTTGTATGTCATT
TTATCCCATTGTCCCCAATTACTAAAACAAGACAAAATTGTAAAAACAATCCTCTACTTG
TAATTCTAGGTAGCACAGGATAAAAAATATTTGCATATTTTGGCCAGGCATGGTGGCTCA
CGCCTGTAATCCCAGCACTTAGGGAGGCCGAGGCAGGTGGATCACTTGAGGTCAGAAGTT
CGAGACCAGCCTGGCCAACATGGTGAAACCCCATCTCTACAAAAATACAAAAAAATTAGC
TGGTCACGGTGGCGCATGCCTGTAATCCCAGTTACTCGGGAGGCTGAGACAGGAGAATCA
CCTGAATCCAGGAGGCGGAAGTTGCAATGAGACACGATCGTGCCATTGCACTCCAGCCTA
CGTGACACAGCAAGACTTCATCTCAAAAAAAAAAAAAGTACATATTTTTAAATCCAGCAG
ATAAATGTGAAATGTTTTGATTACCCATCATTAAATTTTTTAAAAAACTATCCCTACTTC
AGCTGTGGNAAAAATTATCTGGCAACTTCAGGTGACAGATAACTCTGTTGTTTGGTTTAA
AAAN
Restriction Sites NotI-NotI     
ACCN NM_012463
ORF Size 2571 bp
Insert Size 4540
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Reference Data
RefSeq NM_012463.2, NP_036595.2
RefSeq Size 4681
RefSeq ORF 2571
Locus ID 23545
Domains V_ATPase_sub_a
Protein Families Transmembrane
Protein Pathways Epithelial cell signaling in Helicobacter pylori infection, Lysosome, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
Gene Summary The protein encoded by this gene is a subunit of the vacuolar ATPase (v-ATPase), an heteromultimeric enzyme that is present in intracellular vesicles and in the plasma membrane of specialized cells, and which is essential for the acidification of diverse cellular components. V-ATPase is comprised of a membrane peripheral V(1) domain for ATP hydrolysis, and an integral membrane V(0) domain for proton translocation. The subunit encoded by this gene is a component of the V(0) domain. Mutations in this gene are a cause of both cutis laxa type II and wrinkly skin syndrome. [provided by RefSeq, Jul 2009]

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