SPDEF (NM_012391) Human Untagged Clone

CAT#: SC115408

SPDEF (untagged)-Human SAM pointed domain containing ets transcription factor (SPDEF)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SPDEF
• Synonyms: bA375E1.3; PDEF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012391 edited
  GAATTCGGCACGAGGCTGACTTCCTCCCAGCACATTCCTGCACTCTGCCGTGTCCACACT
  GCCCCACAGACCCAGTCCTCCAAGCCTGCTGCCAGCTCCCTGCAAGCCCCTCAGGTTGGG
  CCTTGCCACGGTGCCAGCAGGCAGCCCTGGGCTGGGGGTAGGGGACTCCCTACAGGCACG
  CAGCCCTGAGACCTCAGAGGGCCACCCCTTGAGGGTGGCCAGGCCCCCAGTGGCCAACCT
  GAGTGCTGCCTCTGCCACCAGCCCTGCTGGCCCCTGGTTCCGCTGGCCCCCCAGATGCCT
  GGCTGAGACACGCCAGTGGCCTCAGCTGCCCACACCTCTTCCCGGCCCCTGAAGTTGGCA
  CTGCAGCAGACAGCTCCCTGGGCACCAGGCAGCTAACAGACACAGCCGCCAGCCCAAACA
  GCAGCGGCATGGGCAGCGCCAGCCCGGGTCTGAGCAGCGTATCCCCCAGCCACCTCCTGC
  TGCCCCCCGACACGGTGTCGCGGACAGGCTTGGAGAAGGCGGCAGCGGGGGCAGTGGGTC
  TCGAGAGACGGGACTGGAGTCCCAGTCCACCCGCCACGCCCGAGCAGGGCCTGTCCGCCT
  TCTACCTCTCCTACTTTGACATGCTGTACCCTGAGGACAGCAGCTGGGCAGCCAAGGCCC
  CTGGGGCCAGCAGTCGGGAGGAGCCACCTGAGGAGCCTGAGCAGTGCCCGGTCATTGACA
  GCCAAGCCCCAGCGGGCAGCCTGGACTTGGTGCCCGGCGGGCTGACCTTGGAGGAGCACT
  CGCTGGAGCAGGTGCAGTCCATGGTGGTGGGCGAAGTGCTCAAGGACATCGAGACGGCCT
  GCAAGCTGCTCAACATCACCGCAGATCCCATGGACTGGAGCCCCAGCAATGTGCAGAAGT
  GGCTCCTGTGGACAGAGCACCAATACCGGCTGCCCCCCATGGGCAAGGCCTTCCAGGAGC
  TGGCGGGCAAGGAGCTGTGCGCCATGTCGGAGGAGCAGTTCCGCCAGCGCTCGCCCCTGG
  GTGGGGATGTGCTGCACGCCCACCTGGACATCTGGAAGTCAGCGGCCTGGATGAAAGAGC
  GGACTTCACCTGGGGCGATTCACTACTGTGCCTCGACCAGTGAGGAGAGCTGGACCGACA
  GCGAGGTGGACTCATCATGCTCCGGGCAGCCCATCCACCTGTGGCAGTTCCTCAAGGAGT
  TGCTACTCAAGCCCCACAGCTATGGCCGCTTCATTAGGTGGCTCAACAAGGAGAAGGGCA
  TCTTCAAAATTGAGGACTCAGCCCAGGTGGCCCGGCTGTGGGGCATCCGCAAGAACCGTC
  CCGCCATGAACTACGACAAGCTGAGCCGCTCCATCCGCCAGTATTACAAGAAGGGCATCA
  TCCGGAAGCCAGACATCTCCCAGCGCCTCGTCTACCAGTTCGTGCACCCCATCTGAGTGC
  CTGGCCCAGGGCCTGAAACCCGCCCTCAGGGGCCTCTCTCCTGCCTGCCCTGCCTCAGCC
  AGGCCCTGAGATGGGGGAAAACGGGCAGTCTGCTCTGCTGCTCTGACCTTCCAGAGCCCA
  AGGTCAGGGAGGGGCAACCAACTGCCCCAGGGGGATATGGGTCCTCTGGGGCCTTCGGGA
  CCCTGGGGCAGGGGTGCTTCCTCCTCAGGCCCAGCTGCTCCCCTGGAGGACAGAGGGAGA
  CAGGGCTGCTCCCCAACACCTGCCTCTGACCCCAGCATTTCCAGAGCAGAGCCTACAGAA
  GGGCAGTGACTCGACAAAGGCCACAGGCAGTCCAGGCCTCTCTCTGCTCCATCCCCCTGC
  CTCCCATTCTGCACCACACCTGGCATGGTGCAGGGAGACATCTGCACCCCTGAGTTGGGC
  AGCCAGGAGTGCCCCCGGGAATGGATAATAAAGATACTAGAGAACTGAAAAAAAAAAAAA
  AAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012391 unedited
  GTTCAAAATTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCTGACTTCCT
  CCCAGCACATTCCTGCACTCTGCCGTGTCCACACTGCCCCACAGACCCAGTCCTCCAAGC
  CTGCTGCCAGCTCCCTGCAAGCCCCTCAGGTTGGGCCTTGCCACGGTGCCAGCAGGCAGC
  CCTGGGCTGGGGGTAGGGGACTCCCTACAGGCACGCAGCCCTGAGACCTCAGAGGGCCAC
  CCCTTGAGGGTGGCCAGGCCCCCAGTGGCCAACCTGAGTGCTGCCTCTGCCACCAGCCCT
  GCTGGCCCCTGGTTCCGCTGGCCCCCCAGATGCCTGGCTGAGACACGCCAGTGGCCTCAG
  CTGCCCACACCTCTTCCCGGCCCCTGAAGTTGGCACTGCAGCAGACAGCTCCCTGGGCAC
  CAGGCAGCTAACAGACACAGCCGCCAGCCCAAACAGCAGCGGCATGGGCAGCGCCAGCCC
  GGGTCTGAGCAGCGTATCCCCCAGCCACCTCCTGCTGCCCCCCGACACGGTGTCGCGGAC
  AGGCTTGGAGAAGGCGGCAGCGGGGGCAGTGGGTCTCGAGAGACGGGACTGGAGTCCCAG
  TCCACCCGCCACGCCCGAGCAGGGCCTGTCCGCCTTCTACCTCTCCTACTTTGACATGCT
  GTACCCTGAGGACAGCAGCTGGGCAGCCAAGGCCCCTGGGGCCAGCAGTCGGGAGGAGCC
  ACCTGAGGAGCCTGAGCAGTGCCCGGTCATTGACAGCCAAGCCCCAGCGGGCAGCCTGGA
  CTTGGTGCCCGGCGGGCTGACCTTGGAGGAGCACTCGCTGGAGCAGGTGCCATTCATGGG
  TGGTGGGCGAAGTGCTCAAGGACTCGAGACGGCCTGCAAGCTGCTAAAATTCACCGCAGT
  TCCATGGACTGGAGCCCCACCATGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012391 unedited
  ACGCGGCCGCATTTTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTACAGTTCTCTAGTAT
  CTTTATTATCCATTCCCGGGGGCACTCCTGGCTGCCCAACTCAGGGGTGCAGATGTCTCC
  CTGCACCATGCCAGGTGTGGTGCAAAATGGGAGGCAGGGGGATGGAGCAGAGAGAGGCCT
  GGACTGCCTGTGGCCTTTGTCGAGTCACTGCCCTTCTGTAGGCTCTGCTCTGGAAATGCT
  GGGGTCAAAGGCAGGTGTTGGGGAGCAGCCCTGTCTCCCTCTGTCCTCCAGGGGAGCAGC
  TGGGCCTGAGGAGGAAGCACCCCTGCCCCAGGGTCCCGAAGGCCCCAGAGGACCCATATC
  CCCCTGGGGCAGTTGGTTGCCCCTCCCTGACCTTGGGCTCTGGAAGGTCAGAGCAGCAGA
  GCAGACTGCCCGTTTTCCCCCATCTCAGGGCCTGTCTGAGGCAGGGCAGGCAGGAGAGAG
  GCCCCTGAGGGCGGGTTTCAGGCCCTGGGCCAGGCACTCAGATGGGGTGCACGAACTGGT
  AAACGAGGCGCTGGGAGATGCCTGGCTTCCGGATGATGCCCTTCTTGTAATACTGGCGGA
  TGGAGCGGCTCACCTTGCTCCACTTTATGGTGGGACGGTTCCTGCGGATGCCCCACAGCC
  GGGCCACCTGGGCTGAGTCCTCAATTTTGAAGATGCCCTTCTCCTTGTTGAGCCATCTAT
  TGAAGCGGCCCTAGCTGCGCGGCTTGAGTACAACTCCCTGTAGAACTGCCCAGGTGGATG
  GGCTCGCCGAAACATGATGAGTCACCTCGCTGCCGGTCCAAGTTTCTCACTGGACGAAGG
  AAAGTATTGATTGCCCCAGGGGAACCCCGTTCTTTTTTCCAGCCGCTGACTTCCAATGTA
  CAGGCGGGCGCCCCAACATTCCCACCGAGCGGACGCCTGTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012391
• ORF Size: 1008 bp
• Insert Size: 2100
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_012391.1, NP_036523.1
• RefSeq Size: 1894
• RefSeq ORF: 1008
• Locus ID: 25803
• Domains: ETS, SAM_PNT
• Protein Families: Transcription Factors
• Gene Summary: The protein encoded by this gene belongs to the ETS family of transcription factors. It is highly expressed in the prostate epithelial cells, and functions as an androgen-independent transactivator of prostate-specific antigen (PSA) promoter. Higher expression of this protein has also been reported in brain, breast, lung and ovarian tumors, compared to the corresponding normal tissues, and it shows better tumor-association than other cancer-associated molecules, making it a more suitable target for developing specific cancer therapies. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
  Transcript Variant: This variant (1) encodes the longer isoform (1).