BTK (NM_000061) Human Untagged Clone

CAT#: SC115442

BTK (untagged)-Human Bruton agammaglobulinemia tyrosine kinase (BTK)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BTK
• Synonyms: AGMX1; AT; ATK; BPK; IGHD3; IMD1; PSCTK1; XLA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000061 edited
  ATGGCCGCAGTGATTCTGGAGAGCATCTTTCTGAAGCGATCCCAACAGAAAAAGAAAACA
  TCACCTCTAAACTTCAAGAAGCGCCTGTTTCTCTTGACCGTGCACAAACTCTCCTACTAT
  GAGTATGACTTTGAACGTGGGAGAAGAGGCAGTAAGAAGGGTTCAATAGATGTTGAGAAG
  ATCACTTGTGTTGAAACAGTGGTTCCTGAAAAAAATCCTCCTCCAGAAAGACAGATTCCG
  AGAAGAGGTGAAGAGTCCAGTGAAATGGAGCAAATTTCAATCATTGAAAGGTTCCCTTAT
  CCCTTCCAGGTTGTATATGATGAAGGGCCTCTCTACGTCTTCTCCCCAACTGAAGAACTA
  AGGAAGCGGTGGATTCACCAGCTCAAAAACGTAATCCGGTACAACAGTGATCTGGTTCAG
  AAATATCACCCTTGCTTCTGGATCGATGGGCAGTATCTCTGCTGCTCTCAGACAGCCAAA
  AATGCTATGGGCTGCCAAATTTTGGAGAACAGGAATGGAAGCTTAAAACCTGGGAGTTCT
  CACCGGAAGACAAAAAAGCCTCTTCCCCCAACGCCTGAGGAGGACCAGATCTTGAAAAAG
  CCACTACCGCCTGAGCCAGCAGCAGCACCAGTCTCCACAAGTGAGCTGAAAAAGGTTGTG
  GCCCTTTATGATTACATGCCAATGAATGCAAATGATCTACAGCTGCGGAAGGGTGATGAA
  TATTTTATCTTGGAGGAAAGCAACTTACCATGGTGGAGAGCACGAGATAAAAATGGGCAG
  GAAGGCTACATTCCTAGTAACTATGTCACTGAAGCAGAAGACTCCATAGAAATGTATGAG
  TGGTATTCCAAACACATGACTCGGAGTCAGGCTGAGCAACTGCTAAAGCAAGAGGGGAAA
  GAAGGAGGTTTCATTGTCAGAGACTCCAGCAAAGCTGGCAAATATACAGTGTCTGTGTTT
  GCTAAATCCACAGGGGACCCTCAAGGGGTGATACGTCATTATGTTGTGTGTTCCACACCT
  CAGAGCCAGTATTACCTGGCTGAGAAGCACCTTTTCAGCACCATCCCTGAGCTCATTAAC
  TACCATCAGCACAACTCTGCAGGACTCATATCCAGGCTCAAATATCCAGTGTCTCAACAA
  AACAAGAATGCACCTTCCACTGCAGGCCTGGGATACGGATCATGGGAAATTGATCCAAAG
  GACCTGACCTTCTTGAAGGAGCTGGGGACTGGACAATTTGGGGTAGTGAAGTATGGGAAA
  TGGAGAGGCCAGTACGACGTGGCCATCAAGATGATCAAAGAAGGCTCCATGTCTGAAGAT
  GAATTCATTGAAGAAGCCAAAGTCATGATGAATCTTTCCCATGAGAAGCTGGTGCAGTTG
  TATGGCGTCTGCACCAAGCAGCGCCCCATCTTCATCATCACTGAGTACATGGCCAATGGC
  TGCCTCCTGAACTACCTGAGGGAGATGCGCCACCGCTTCCAGACTCAGCAGCTGCTAGAG
  ATGTGCAAGGATGTCTGTGAAGCCATGGAATACCTGGAGTCAAAGCAGTTCCTTCACCGA
  GACCTGGCAGCTCGAAACTGTTTGGTAAACGATCAAGGAGTTGTTAAAGTATCTGATTTC
  GGCCTGTCCAGGTATGTCCTGGATGATGAATACACAAGCTCAGTAGGCTCCAAATTTCCA
  GTCCGGTGGTCCCCACCGGAAGTCCTGATGTATAGCAAGTTCAGCAGCAAATCTGACATT
  TGGGCTTTTGGGGTTTTGATGTGGGAAATTTACTCCCTGGGGAAGATGCCATATGAGAGA
  TTTACTAACAGTGAGACTGCTGAACACATTGCCCAAGGCCTACGTCTCTACAGGCCTCAT
  CTGGCTTCAGAGAAGGTATATACCATCATGTACAGTTGCTGGCATGAGAAAGCAGATGAG
  CGTCCCACTTTCAAAATTCTTCTGAGCAATATTCTAGATGTCATGGATGAAGAATCCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000061 unedited
  TTCAAATTTGTATACGACTCCTATAGGGCGGCCGCGATTCGGCACGAGGCCAGGGCCAGT
  GTCTGCTGCGATCGAGTCCCACCTTCCAAGTCCTGGCATCTCAATGCATCTGGGAAGCTA
  CCTGCATTAAGTCAGGACTGAGCACACAGGTGAACTCCAGAAAGAAGAAGCTATGGCCGC
  AGTGATTCTGGAGAGCATCTTTCTGAAGCGATCCCAACAGAAAAAGAAAACATCACCTCT
  AAACTTCAAGAAGCGCCTGTTTCTCTTGACCGTGCACAAACTCTCCTACTATGAGTATGA
  CTTTGAACGTGGGAGAAGAGGCAGTAAGAAGGGTTCAATAGATGTTGAGAAGATCACTTG
  TGTTGAAACAGTGGTTCCTGAAAAAAATCCTCCTCCAGAAAGACAGATTCCGAGAAGAGG
  TGAAGAGTCCAGTGAAATGGAGCAAATTTCAATCATTGAAAGGTTCCCTTATCCCTTCCA
  GGTTGTATATGATGAAGGGCCTCTCTACGTCTTCTCCCCAACTGAAGAACTAAGGAAGCG
  GTGGATTCACCAGCTCAAAAACGTAATCCGGTACAACAGTGATCTGGTTCAGAAATATCA
  CCCTTGCTTCTGGATCGATGGGCAGTATCTCTGCTGCTCTCAGACAGCCAAAAATGCTAT
  GGGCTGCCAAATTTTGGAGAACAGGAATGGAAGCTTANNACCTGGNGAGTCTCACCGGAA
  GACANAAAAGCCTCTTCCCCCAACGCCTNGAGAGGACCAGATCTTTGAAAGCCACTACCG
  NCTGAGCCAGCAGCAGCACCAGTCTCCACCAGTGAGCTGAAAAAGTTGTGGCCCCTTATG
  ATTACATGCCCNATGATGCANATGATCTACAGCTGCCGGAGGGTGATGAATATTTATCTT
  GGNAGAAAAGCACTTACATGGTGGAGAGCCCGAGAAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000061 unedited
  GCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTCAAGCTTTCTAGTAATTTTATTT
  TATCAAAACACCCTCCCCTCCCATCTTTATGACACCATTTCATTCTTGCCAAATTCGGTC
  CACCCCCACACACACACCCCCAAGCTTTACCAAATGCCTACTCAAAGAGCTGTTGGACCC
  CTTTGTGCGGCTATTTACATCCTCCCTCCTAAAAAATATTTTCATCGCAAATTCAGTCTG
  TCTTAATTCTCTCGGGAAATTTCAGGCACAATAATTTCTTGGCCTTTGCTCAAAAGCCAC
  TATCCCAGGGAATCAAAGAAGAGGTGCATTCCCAGATGTAAAGAGGGGCCTTTGTGTATT
  GAGTGGGAGCACAAAGGCTCCAGGGCTCCTAAGCTTGGGATTTCCTCTGAGAAAGTGAAA
  TTGGGGCTTGTGGAGAAGAGAAGTANAACCAAGAAGCTTATTGGCGAGCTCAGGATTCTT
  CATCCATGACATCTAGAATATTGCTCAGAAGAATTTTGAAAGTGGGACGCTCATCTGCTT
  TCTCATGCCAGCAACTGTACATGATGGTATATACCTTCTCTGAAGCCAGATGAGGCCTGT
  ANAGACGTAGGCCTTGGGCAATGTGTTCAGCAGTCTCACTGTTAGTAAAATCTCTATATG
  GCATCTTCCCCAGGGAGTAAATTTCCCCACATAAAACCCCAAAAGCCCAAAGCCAGATTT
  GCTGCTGAACTTGTATACATCAGGACTTCCGTGGGGACCACCGGACTGGAAATTTGGACG
  CTACTGAGCTTGGGTATTCACATCCCGGACACTCCTGCACAGCGCGAAATAGATACTTTT
  ACACTTCCTTGACCGTTAACAAACAAGTTCAACCGTCAAGCCTCGTGGAGGGACCGCTTC
  CACCTCCGGTTCCCAGGGTTTCCACCATCCTTGCCCACCTATCATCGGCTGCCTCGGAGC
  GGGGCCGCTCCCCTCAGCAGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000061
• Insert Size: 2790 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000061.1, NP_000052.1
• RefSeq Size: 2591 bp
• RefSeq ORF: 1980 bp
• Locus ID: 695
• Cytogenetics: Xq22.1
• Domains: pkinase, SH2, TyrKc, SH3, BTK, PH, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: B cell receptor signaling pathway, Fc epsilon RI signaling pathway, Primary immunodeficiency
• Gene Summary: 'The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013]'
  Transcript Variant: This variant (1) encodes isoform 1.