LETM1 (NM_012318) Human Untagged Clone

CAT#: SC115448

LETM1 (untagged)-Human leucine zipper-EF-hand containing transmembrane protein 1 (LETM1), nuclear gene encoding mitochondrial protein


  "NM_012318" in other vectors (7)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol LETM1
Synonyms SLC55A1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC115448 sequence for NM_012318 edited (data generated by NextGen Sequencing)
ATGGCGTCCATCTTACTGAGGAGCTGCCGCGGCCGGGCGCCCGCCCGCCTCCCGCCGCCG
CCTCGGTACACCGTCCCGCGGGGTAGTCCAGGGGATCCTGCTCATCTCAGCTGTGCCAGC
ACCCTGGGGTTGAGGAACTGCCTGAATGTTCCATTTGGCTGCTGCACTCCCATCCACCCT
GTGTACACATCCTCCAGAGGCGATCACCTCGGCTGTTGGGCTCTGAGGCCCGAGTGCCTT
CGCATAGTGTCGAGAGCGCCATGGACCTCTACCTCTGTGGGTTTTGTGGCTGTGGGACCT
CAGTGCCTTCCTGTGCGTGGCTGGCACTCTTCGCGCCCTGTTCGCGATGACTCGGTAGTA
GAGAAGTCCCTCAAGTCCTTGAAGGACAAGAACAAGAAGCTGGAGGAAGGCGGCCCGGTG
TACAGCCCCCCCGCAGAGGTGGTGGTGAAGAAGTCCCTGGGGCAGCGGGTGCTGGACGAG
CTGAAGCACTACTACCATGGCTTCCGCCTGCTATGGATCGACACCAAGATCGCGGCACGC
ATGCTCTGGCGCATCCTCAACGGCCACAGCCTGACCCGCCGGGAGCGCAGGCAGTTTCTC
CGGATCTGCGCTGACCTCTTCCGCCTGGTGCCGTTCCTTGTGTTCGTGGTGGTGCCGTTC
ATGGAGTTTCTGCTGCCTGTTGCTGTGAAGCTCTTCCCCAACATGTTGCCATCCACATTT
GAGACTCAGTCACTCAAGGAGGAGAGGCTGAAGAAGGAGCTTCGGGTCAAGCTGGAGCTG
GCCAAGTTCCTCCAGGACACCATCGAGGAGATGGCCTTGAAGAACAAGGCAGCCAAGGGC
AGCGCCACCAAAGACTTCTCTGTGTTTTTCCAGAAGATCCGGGAAACAGGGGAGAGGCCC
AGCAATGAGGAAATCATGCGTTTTTCCAAATTATTTGAGGATGAGCTGACCCTGGACAAC
CTGACACGGCCGCAGCTGGTGGCCCTGTGCAAGCTGCTGGAGCTACAGTCCATCGGCACC
AACAACTTCCTGCGCTTCCAGCTTACCATGCGGCTGCGCTCCATAAAGGCAGACGACAAG
CTGATTGCTGAGGAAGGGGTGGACAGCCTGAATGTCAAGGAGCTGCAGGCAGCGTGTCGG
GCACGAGGCATGCGGGCCCTGGGCGTCACGGAAGACCGCCTGAGGGGTCAGCTGAAGCAG
TGGCTGGACCTGCACCTGCATCAGGAGATCCCCACATCGCTGCTCATCCTGTCCCGGGCC
ATGTACCTCCCGGACACCCTCTCTCCAGCCGACCAGCTCAAGTCCACACTGCAGACCCTC
CCAGAGATTGTGGCAAAGGAAGCACAGGTGAAAGTGGCCGAGGTGGAGGGCGAGCAGGTG
GACAACAAGGCCAAGCTGGAGGCCACGCTGCAGGAGGAGGCGGCCATCCAGCAGGAGCAC
CGTGAGAAGGAGCTGCAGAAGCGCTCGGAGGTGGCGAAGGATTTTGAGCCCGAACGTGTG
GTAGCTGCTCCCCAAAGGCCGGGGACCGAGCCACAGCCAGAAATGCCTGACACAGTCCTG
CAGTCAGAGACCTTGAAGGACACTGCCCCGGTGCTGGAGGGCTTGAAGGAGGAAGAGATC
ACGAAGGAGGAAATCGACATCCTCAGCGATGCCTGCTCTAAGCTGCAGGAGCAGAAGAAG
TCACTCACCAAGGAGAAGGAGGAGCTGGAGCTGCTGAAGGAGGATGTGCAGGACTACAGC
GAGGACTTGCAGGAGATCAAGAAGGAACTTTCAAAGACTGGTGAAGAAAAATACGTGGAA
GAATCTAAAGCCAGCAAGAGATTGACAAAAAGGGTGCAGCAAATGATCGGGCAGATCGAT
GGCTTGATCTCGCAGCTGGAGATGGACCAGCAGGCTGGCAAGCTGGCCCCGGCCAACGGC
ATGCCCACGGGGGAGAACGTCATCAGTGTCGCTGAGCTCATCAACGCCATGAAGCAAGTC
AAGCACATTCCCGAAAGCAAGCTCACCAGCCTGGCCGCAGCACTGGATGAAAACAAGGAT
GGCAAGGTCAACATCGACGACCTCGTCAAGGTGATTGAGCTGGTGGACAAAGAAGATGTT
CACATCTCCACCAGCCAGGTGGCTGAGATTGTAGCAACACTGGAAAAAGAGGAGAAGGTG
GAGGAGAAGGAGAAGGCCAAAGAGAAGGCAGAGAAGGAGGTCGCAGAGGTGAAGAGCTAG

Clone variation with respect to NM_012318.2
1791 g=>a
>OriGene 5' read for NM_012318 unedited
NGGTCAAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGCGCTGA
GGGGCCCAATGGGAGCCGCGGCCTGGCCGCCCCGCCCCGCGCCTCCGGACGTCTGTGCCG
GGACAAGGCCGCCGCTGGTGCCGGGTCCTTGAGGAGAGCGCCTCCCGTCCGAGGCCAGCC
GCCTCTGTCAGCCGTCCGCGCGGGCCGGGTCTGAAGCGCCGCCGGGACGGCGAAGAGCCG
CGGCCGCCGCGGAGAAGGAGGCAGCGCAGGAGGCCGGAGCGGCCGCCGCGCCCGAGCACA
TGGCGTCCATCTTACTGAGGAGCTGCCGCGGCCGGGCGCCCGCCCGCCTCCCGCCGCCGC
CTCGGTACACCGTCCCGCGGGGTAGTCCAGGGGATCCTGCTCATCTCAGCTGTGCCAGCA
CCCTGGGGTTGAGGAACTGCCTGAATGTTCCATTTGGCTGCTGCACTCCCATCCACCCTG
TGTACACATCCTCCAGAGGCGATCACCTCGGCTGTTGGGCTCTGAGGCCCGAGTGCCTTC
GCATAGTGTCGAGAGCGCCATGGACCTCTACCTCTGTGGNGTTTTGTGGNCTGTGGGACC
TCAGTGCCTTCCTGTGCGTGGCTGGCACTCTTCGCGCCCTGTTCGCGATGACTCGGTAGT
AAAAGAAGTCCCTCAAGTCCTTGAAGGACAAGAACAAGAAGCTGGAGGAAGGCGGCCCCG
GTGTACAGCCCCCCCGCANAGGTGGGTGGTGGAAGAAGTCCCCTGGGCCAACCGGGTGCT
TGGACGANCTGAAGCCCTACTACCATGGCTTCCGCCTGTTATGGATCGACACCAAGATCG
CGGTACGCATGCTTTGGCGCATCCTCAACGGGCACAGCCTGACCCGCCGGNAGCCGCCAG
CAGTTCTCCCGGATCTGC
Restriction Sites NotI-NotI     
ACCN NM_012318
Insert Size 4470 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_012318.1, NP_036450.1
RefSeq Size 3907 bp
RefSeq ORF 2220 bp
Locus ID 3954
Cytogenetics 4p16.3
Protein Families Transmembrane
Gene Summary 'This gene encodes a protein that is localized to the inner mitochondrial membrane. The protein functions to maintain the mitochondrial tubular shapes and is required for normal mitochondrial morphology and cellular viability. Mutations in this gene cause Wolf-Hirschhorn syndrome, a complex malformation syndrome caused by the deletion of parts of the distal short arm of chromosome 4. Related pseudogenes have been identified on chromosomes 8, 15 and 19. [provided by RefSeq, Oct 2009]'

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