LETM1 (NM_012318) Human Untagged Clone

CAT#: SC115448

LETM1 (untagged)-Human leucine zipper-EF-hand containing transmembrane protein 1 (LETM1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LETM1
• Synonyms: SLC55A1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115448 sequence for NM_012318 edited (data generated by NextGen Sequencing)
  ATGGCGTCCATCTTACTGAGGAGCTGCCGCGGCCGGGCGCCCGCCCGCCTCCCGCCGCCG
  CCTCGGTACACCGTCCCGCGGGGTAGTCCAGGGGATCCTGCTCATCTCAGCTGTGCCAGC
  ACCCTGGGGTTGAGGAACTGCCTGAATGTTCCATTTGGCTGCTGCACTCCCATCCACCCT
  GTGTACACATCCTCCAGAGGCGATCACCTCGGCTGTTGGGCTCTGAGGCCCGAGTGCCTT
  CGCATAGTGTCGAGAGCGCCATGGACCTCTACCTCTGTGGGTTTTGTGGCTGTGGGACCT
  CAGTGCCTTCCTGTGCGTGGCTGGCACTCTTCGCGCCCTGTTCGCGATGACTCGGTAGTA
  GAGAAGTCCCTCAAGTCCTTGAAGGACAAGAACAAGAAGCTGGAGGAAGGCGGCCCGGTG
  TACAGCCCCCCCGCAGAGGTGGTGGTGAAGAAGTCCCTGGGGCAGCGGGTGCTGGACGAG
  CTGAAGCACTACTACCATGGCTTCCGCCTGCTATGGATCGACACCAAGATCGCGGCACGC
  ATGCTCTGGCGCATCCTCAACGGCCACAGCCTGACCCGCCGGGAGCGCAGGCAGTTTCTC
  CGGATCTGCGCTGACCTCTTCCGCCTGGTGCCGTTCCTTGTGTTCGTGGTGGTGCCGTTC
  ATGGAGTTTCTGCTGCCTGTTGCTGTGAAGCTCTTCCCCAACATGTTGCCATCCACATTT
  GAGACTCAGTCACTCAAGGAGGAGAGGCTGAAGAAGGAGCTTCGGGTCAAGCTGGAGCTG
  GCCAAGTTCCTCCAGGACACCATCGAGGAGATGGCCTTGAAGAACAAGGCAGCCAAGGGC
  AGCGCCACCAAAGACTTCTCTGTGTTTTTCCAGAAGATCCGGGAAACAGGGGAGAGGCCC
  AGCAATGAGGAAATCATGCGTTTTTCCAAATTATTTGAGGATGAGCTGACCCTGGACAAC
  CTGACACGGCCGCAGCTGGTGGCCCTGTGCAAGCTGCTGGAGCTACAGTCCATCGGCACC
  AACAACTTCCTGCGCTTCCAGCTTACCATGCGGCTGCGCTCCATAAAGGCAGACGACAAG
  CTGATTGCTGAGGAAGGGGTGGACAGCCTGAATGTCAAGGAGCTGCAGGCAGCGTGTCGG
  GCACGAGGCATGCGGGCCCTGGGCGTCACGGAAGACCGCCTGAGGGGTCAGCTGAAGCAG
  TGGCTGGACCTGCACCTGCATCAGGAGATCCCCACATCGCTGCTCATCCTGTCCCGGGCC
  ATGTACCTCCCGGACACCCTCTCTCCAGCCGACCAGCTCAAGTCCACACTGCAGACCCTC
  CCAGAGATTGTGGCAAAGGAAGCACAGGTGAAAGTGGCCGAGGTGGAGGGCGAGCAGGTG
  GACAACAAGGCCAAGCTGGAGGCCACGCTGCAGGAGGAGGCGGCCATCCAGCAGGAGCAC
  CGTGAGAAGGAGCTGCAGAAGCGCTCGGAGGTGGCGAAGGATTTTGAGCCCGAACGTGTG
  GTAGCTGCTCCCCAAAGGCCGGGGACCGAGCCACAGCCAGAAATGCCTGACACAGTCCTG
  CAGTCAGAGACCTTGAAGGACACTGCCCCGGTGCTGGAGGGCTTGAAGGAGGAAGAGATC
  ACGAAGGAGGAAATCGACATCCTCAGCGATGCCTGCTCTAAGCTGCAGGAGCAGAAGAAG
  TCACTCACCAAGGAGAAGGAGGAGCTGGAGCTGCTGAAGGAGGATGTGCAGGACTACAGC
  GAGGACTTGCAGGAGATCAAGAAGGAACTTTCAAAGACTGGTGAAGAAAAATACGTGGAA
  GAATCTAAAGCCAGCAAGAGATTGACAAAAAGGGTGCAGCAAATGATCGGGCAGATCGAT
  GGCTTGATCTCGCAGCTGGAGATGGACCAGCAGGCTGGCAAGCTGGCCCCGGCCAACGGC
  ATGCCCACGGGGGAGAACGTCATCAGTGTCGCTGAGCTCATCAACGCCATGAAGCAAGTC
  AAGCACATTCCCGAAAGCAAGCTCACCAGCCTGGCCGCAGCACTGGATGAAAACAAGGAT
  GGCAAGGTCAACATCGACGACCTCGTCAAGGTGATTGAGCTGGTGGACAAAGAAGATGTT
  CACATCTCCACCAGCCAGGTGGCTGAGATTGTAGCAACACTGGAAAAAGAGGAGAAGGTG
  GAGGAGAAGGAGAAGGCCAAAGAGAAGGCAGAGAAGGAGGTCGCAGAGGTGAAGAGCTAG
  
  Clone variation with respect to NM_012318.2
  1791 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012318 unedited
  NGGTCAAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGGCGCTGA
  GGGGCCCAATGGGAGCCGCGGCCTGGCCGCCCCGCCCCGCGCCTCCGGACGTCTGTGCCG
  GGACAAGGCCGCCGCTGGTGCCGGGTCCTTGAGGAGAGCGCCTCCCGTCCGAGGCCAGCC
  GCCTCTGTCAGCCGTCCGCGCGGGCCGGGTCTGAAGCGCCGCCGGGACGGCGAAGAGCCG
  CGGCCGCCGCGGAGAAGGAGGCAGCGCAGGAGGCCGGAGCGGCCGCCGCGCCCGAGCACA
  TGGCGTCCATCTTACTGAGGAGCTGCCGCGGCCGGGCGCCCGCCCGCCTCCCGCCGCCGC
  CTCGGTACACCGTCCCGCGGGGTAGTCCAGGGGATCCTGCTCATCTCAGCTGTGCCAGCA
  CCCTGGGGTTGAGGAACTGCCTGAATGTTCCATTTGGCTGCTGCACTCCCATCCACCCTG
  TGTACACATCCTCCAGAGGCGATCACCTCGGCTGTTGGGCTCTGAGGCCCGAGTGCCTTC
  GCATAGTGTCGAGAGCGCCATGGACCTCTACCTCTGTGGNGTTTTGTGGNCTGTGGGACC
  TCAGTGCCTTCCTGTGCGTGGCTGGCACTCTTCGCGCCCTGTTCGCGATGACTCGGTAGT
  AAAAGAAGTCCCTCAAGTCCTTGAAGGACAAGAACAAGAAGCTGGAGGAAGGCGGCCCCG
  GTGTACAGCCCCCCCGCANAGGTGGGTGGTGGAAGAAGTCCCCTGGGCCAACCGGGTGCT
  TGGACGANCTGAAGCCCTACTACCATGGCTTCCGCCTGTTATGGATCGACACCAAGATCG
  CGGTACGCATGCTTTGGCGCATCCTCAACGGGCACAGCCTGACCCGCCGGNAGCCGCCAG
  CAGTTCTCCCGGATCTGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012318
• Insert Size: 4470 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012318.1, NP_036450.1
• RefSeq Size: 3907 bp
• RefSeq ORF: 2220 bp
• Locus ID: 3954
• Cytogenetics: 4p16.3
• Protein Families: Transmembrane
• Gene Summary: 'This gene encodes a protein that is localized to the inner mitochondrial membrane. The protein functions to maintain the mitochondrial tubular shapes and is required for normal mitochondrial morphology and cellular viability. Mutations in this gene cause Wolf-Hirschhorn syndrome, a complex malformation syndrome caused by the deletion of parts of the distal short arm of chromosome 4. Related pseudogenes have been identified on chromosomes 8, 15 and 19. [provided by RefSeq, Oct 2009]'