MGAT4A (NM_012214) Human Untagged Clone

CAT#: SC115491

MGAT4A (untagged)-Human mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A (MGAT4A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MGAT4A
• Synonyms: GnT-4a; GNT-IV; GNT-IVA
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115491 sequence for NM_012214 edited (data generated by NextGen Sequencing)
  ATGAGGCTCCGCAATGGAACTGTAGCCACTGCTTTAGCATTTATCACTTCCTTCCTTACT
  TTGTCTTGGTATACTACATGGCAAAATGGGAAAGAAAAACTGATTGCTTATCAACGAGAA
  TTCCTTGCTTTGAAAGAACGTCTTCGAATAGCTGAACACAGAATCTCACAGCGCTCTTCT
  GAATTAAATACGATTGTGCAACAGTTCAAGCGTGTAGGAGCAGAAACAAATGGAAGTAAG
  GATGCGTTGAATAAGTTTTCAGATAATACCCTAAAGCTGTTAAAGGAGTTAACAAGCAAA
  AAATCTCTTCAAGTGCCAAGTATTTATTATCATTTGCCTCATTTATTGAAAAATGAAGGA
  AGTCTTCAACCTGCTGTACAGATTGGCAACGGAAGAACAGGAGTTTCAATAGTCATGGGC
  ATTCCCACAGTGAAGAGAGAAGTTAAATCTTACCTCATAGAAACTCTTCATTCCCTTATT
  GATAACCTGTATCCTGAAGAGAAGTTGGACTGTGTTATAGTAGTCTTCATAGGAGAGACA
  GATATTGATTATGTACATGGTGTTGTAGCCAACCTGGAGAAAGAATTTTCTAAAGAAATC
  AGTTCTGGCTTGGTGGAAGTCATATCACCCCCTGAAAGCTATTATCCTGACTTGACAAAC
  CTAAAGGAGACATTTGGAGACTCCAAAGAAAGAGTAAGATGGAGAACAAAGCAAAACCTA
  GATTACTGTTTTCTAATGATGTATGCTCAAGAAAAGGGCATATATTACATTCAGCTTGAA
  GATGATATTATTGTCAAACAAAATTATTTTAATACCATAAAAAATTTTGCACTTCAACTT
  TCTTCTGAGGAATGGATGATTCTAGAGTTTTCCCAGCTGGGCTTCATTGGTAAAATGTTT
  CAAGCGCCGGATCTTACTCTGATTGTAGAATTCATATTCATGTTTTACAAGGAGAAACCC
  ATTGATTGGCTCCTGGACCATATTCTCTGGGTGAAAGTCTGCAACCCTGAAAAAGATGCA
  AAACATTGTGATAGACAGAAAGCAAATCTGCGAATTCGCTTCAGACCTTCCCTTTTCCAA
  CATGTTGGTCTGCACTCATCACTATCAGGAAAAATCCAAAAACTCACGGATAAAGATTAT
  ATGAAACCATTACTTCTTAAAATCCATGTAAACCCACCTGCGGAGGTATCTACTTCCTTG
  AAGGTCTACCAAGGGCATACGCTGGAGAAAACTTACATGGGAGAGGATTTCTTCTGGGCT
  ATCACACCGATAGCTGGAGACTACATCTTGTTTAAATTTGATAAACCAGTCAATGTAGAA
  AGTTATTTGTTCCATAGCGGCAACCAAGAACATCCTGGAGATATTCTGCTAAACACAACT
  GTGGAAGTTTTGCCTTTTAAGAGTGAAGGTTTGGAAATAAGCAAAGAAACCAAAGACAAA
  CGATTAGAAGATGGCTATTTCAGAATAGGAAAATTTGAGAATGGTGTTGCAGAAGGAATG
  GTGGATCCAAGTCTCAATCCCATTTCAGCCTTTCGACTTTCAGTTATTCAGAATTCTGCT
  GTTTGGGCCATTCTTAATGAGATTCATATTAAAAAAGCCACCAACTGA
  
  Clone variation with respect to NM_012214.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012214 unedited
  GGGGTCAGGAACNGAAAACGACTTACTATAGGCGGCCGCGAATTCGCACGAGGCCAGCGG
  CGGGAAGGGAAAAGGCCGAGGCATCAGCGTGTGAAGACCGCAAAGACGATCCCGAGTACA
  GTTGTGAACAGCATTGCTGCTAGGCTCCTCCTGCAGATCATCTGAAATGAACCTCTCTTA
  TTGATTTTTATTGGCCTAGAGCCAGGAGTACTGCATTCAGTTGACTTTCAGGGTAAAAAG
  AAAACAGTCCTGGTTGTTGTCATCATAAACATATGGACCAGTGTGATGGTGAAATGAGAT
  GAGGCTCCGCAATGGAACTGTAGCCACTGCTTTAGCATTTATCACTTCCTTCCTTACTTT
  GTCTTGGTATACTACATGGCAAAATGGGAAAGAAAAACTGATTGCTTATCAACGAGAATT
  CCTTGCTTTGAAAGAACGTCTTCGAATAGCTGAACACAGAATCTCACAGCGCTCTTCTGA
  ATTAAATACGATTGTGCAACAGTTCAAGCGTGTAGGAGCAGAAACAAATGGAAGTAAGGA
  TGCGTTGAATAAGTTTTCAGATAATACCCTAAAGCTGTTAAAGGAGTTAACAAGCAAAAA
  ATCTCTTCAAGTGCCAAGTATTTATTATCATTTGCCTCATTTATTGAAAAATGAAGGAAG
  TCTTCAACCTGCTGTACAGATTGGCAACGGAAGAACAGGAGTTTCAATAGTCATGGGCAT
  TCCCACAGTGAAGAGAGAAGTTAAATCTTACCTCATAGAAACTCTTCATTCCCTTATTTG
  ATACCTGTATCCTGAAGAGAAGTTGGACTGTGTTATAGTAGTCTTCATAGGAGAGACAGA
  TATTGATTATGTACATGGNTGTCGTAGCCCACCTGGAGAAAGAATTNNTCTAAGNAANAT
  CAGTCTGGCGGGNNGGGAGTCATATCACCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012214 unedited
  GGGGGCACCCCNAGCNCCTTCTANNAAACNGGTAATCTCTGNACCGCGGNCCGCATNCTA
  GGATCGAGNNNTTTTTTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCAGGC
  TAAACTATTTTTAATTAACAAATTCACAGGAAAAAAAGGGGTTGGTTTTCTACAAATGAT
  CCAGTGGGGGGCTTTTTTTAATATGAATCTCATTAAAAAGGGCCCAAACAGCAAAATTTT
  GAATAACTGAAAGTCAAAGGGCTGAAAGGGGATTGAAACTTGAATCCACCTTTCTTTTTG
  CAACACCTTTCTAAAATTTTCCTATTCTGAAATACCCATCTTCTAACCGTTGGCCTTTGG
  TTTCTTTGCTTATTCCCAAACCTTCATTTTTAAAAGGCAAAACTTCCACAGTTGTGTTTA
  CCAAAATATCTCCAGAATGTTCTTGGTTGCCGTTATGGAACAAATAACTTTCTACATTGA
  CGGGTTTATCAAATTTAACCAAGATGTACTCTCCAGCTATCGGTGTGATAGCCCAGAAGA
  AATCCTCTCCATGTAAGTTTTCCCCAGCGTTTGCCCTTGGTAAACCTTCAAGGAAGAAAA
  TACTTCCCCAGGGTGGTTTACCTGGATTTAAAGAANAATGGGTTAATATAATCTTTATCC
  CGGAGTTTTTGGATTTTTCCTGAAGGGAAGAGGGCCAACCAACTGTTGAAAGGGGAGGTT
  TGAACCAAATTCCAAATTGCTTTTTGCTTATCCATGTTTGGCTTTTTTTAGGGTTGGCAA
  CTTTCCCCCAAAAAATGGGCCCAGACCCAACAAGGGGTTTTCCCTGGAAAAAGGAATTGA
  ATTTTCCCCCCCGGAAAAAACCCGGGGTTGGAAACATTTTCCCATGAACCCCCCTTGGGA
  AACCTTTAAATTTTCCCTTTCTTCCAAAAAGGGGGAGGGCCAAAATTTTTATGGGATAAA
  AAAATTTTTGTTGAAAAAAATTCCTCTTCCACCGGAGGGGAAATGGCCTTTTTTTGGCAC
  AACTATTAAAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_012214
• ORF Size: 1608 bp
• Insert Size: 1960
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_012214.1, NP_036346.1
• RefSeq Size: 2115
• RefSeq ORF: 1608
• Locus ID: 11320
• Domains: Glyco_transf_55
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: This gene encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. The encoded protein, in addition to the related isoenzyme B, catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-1,4 linkage to the Man-alpha-1,3-Man-beta-1,4-GlcNAc arm of R-Man-alpha-1,6(GlcNAc-beta-1,2-Man-alpha-1,3)Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1-Asn. The encoded protein may play a role in regulating the availability of serum glycoproteins, oncogenesis, and differentiation. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).