MGAT4A (NM_012214) Human Tagged ORF Clone

CAT#: RG213802

MGAT4A (GFP-tagged) - Human mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A (MGAT4A), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MGAT4A
• Synonyms: GnT-4a; GNT-IV; GNT-IVA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG213802 representing NM_012214
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCTCCGCAATGGAACTGTAGCCACTGCTTTAGCATTTATCACTTCCTTCCTTACTTTGTCTTGGT
  ATACTACATGGCAAAATGGGAAAGAAAAACTGATTGCTTATCAACGAGAATTCCTTGCTTTGAAAGAACG
  TCTTCGAATAGCTGAACACAGAATCTCACAGCGCTCTTCTGAATTAAATACGATTGTGCAACAGTTCAAG
  CGTGTAGGAGCAGAAACAAATGGAAGTAAGGATGCGTTGAATAAGTTTTCAGATAATACCCTAAAGCTGT
  TAAAGGAGTTAACAAGCAAAAAATCTCTTCAAGTGCCAAGTATTTATTATCATTTGCCTCATTTATTGAA
  AAATGAAGGAAGTCTTCAACCTGCTGTACAGATTGGCAACGGAAGAACAGGAGTTTCAATAGTCATGGGC
  ATTCCCACAGTGAAGAGAGAAGTTAAATCTTACCTCATAGAAACTCTTCATTCCCTTATTGATAACCTGT
  ATCCTGAAGAGAAGTTGGACTGTGTTATAGTAGTCTTCATAGGAGAGACAGATATTGATTATGTACATGG
  TGTTGTAGCCAACCTGGAGAAAGAATTTTCTAAAGAAATCAGTTCTGGCTTGGTGGAAGTCATATCACCC
  CCTGAAAGCTATTATCCTGACTTGACAAACCTAAAGGAGACATTTGGAGACTCCAAAGAAAGAGTAAGAT
  GGAGAACAAAGCAAAACCTAGATTACTGTTTTCTAATGATGTATGCTCAAGAAAAGGGCATATATTACAT
  TCAGCTTGAAGATGATATTATTGTCAAACAAAATTATTTTAATACCATAAAAAATTTTGCACTTCAACTT
  TCTTCTGAGGAATGGATGATTCTAGAGTTTTCCCAGCTGGGCTTCATTGGTAAAATGTTTCAAGCGCCGG
  ATCTTACTCTGATTGTAGAATTCATATTCATGTTTTACAAGGAGAAACCCATTGATTGGCTCCTGGACCA
  TATTCTCTGGGTGAAAGTCTGCAACCCTGAAAAAGATGCAAAACATTGTGATAGACAGAAAGCAAATCTG
  CGAATTCGCTTCAGACCTTCCCTTTTCCAACATGTTGGTCTGCACTCATCACTATCAGGAAAAATCCAAA
  AACTCACGGATAAAGATTATATGAAACCATTACTTCTTAAAATCCATGTAAACCCACCTGCGGAGGTATC
  TACTTCCTTGAAGGTCTACCAAGGGCATACGCTGGAGAAAACTTACATGGGAGAGGATTTCTTCTGGGCT
  ATCACACCGATAGCTGGAGACTACATCTTGTTTAAATTTGATAAACCAGTCAATGTAGAAAGTTATTTGT
  TCCATAGCGGCAACCAAGAACATCCTGGAGATATTCTGCTAAACACAACTGTGGAAGTTTTGCCTTTTAA
  GAGTGAAGGTTTGGAAATAAGCAAAGAAACCAAAGACAAACGATTAGAAGATGGCTATTTCAGAATAGGA
  AAATTTGAGAATGGTGTTGCAGAAGGAATGGTGGATCCAAGTCTCAATCCCATTTCAGCCTTTCGACTTT
  CAGTTATTCAGAATTCTGCTGTTTGGGCCATTCTTAATGAGATTCATATTAAAAAAGCCACCAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG213802 representing NM_012214
  Red=Cloning site Green=Tags(s)
  
  MRLRNGTVATALAFITSFLTLSWYTTWQNGKEKLIAYQREFLALKERLRIAEHRISQRSSELNTIVQQFK
  RVGAETNGSKDALNKFSDNTLKLLKELTSKKSLQVPSIYYHLPHLLKNEGSLQPAVQIGNGRTGVSIVMG
  IPTVKREVKSYLIETLHSLIDNLYPEEKLDCVIVVFIGETDIDYVHGVVANLEKEFSKEISSGLVEVISP
  PESYYPDLTNLKETFGDSKERVRWRTKQNLDYCFLMMYAQEKGIYYIQLEDDIIVKQNYFNTIKNFALQL
  SSEEWMILEFSQLGFIGKMFQAPDLTLIVEFIFMFYKEKPIDWLLDHILWVKVCNPEKDAKHCDRQKANL
  RIRFRPSLFQHVGLHSSLSGKIQKLTDKDYMKPLLLKIHVNPPAEVSTSLKVYQGHTLEKTYMGEDFFWA
  ITPIAGDYILFKFDKPVNVESYLFHSGNQEHPGDILLNTTVEVLPFKSEGLEISKETKDKRLEDGYFRIG
  KFENGVAEGMVDPSLNPISAFRLSVIQNSAVWAILNEIHIKKATN
  
  TRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012214
• ORF Size: 1605 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_012214.1, NP_036346.1
• RefSeq Size: 2115
• RefSeq ORF: 1608
• Locus ID: 11320
• Domains: Glyco_transf_55
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: This gene encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. The encoded protein, in addition to the related isoenzyme B, catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-1,4 linkage to the Man-alpha-1,3-Man-beta-1,4-GlcNAc arm of R-Man-alpha-1,6(GlcNAc-beta-1,2-Man-alpha-1,3)Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1-Asn. The encoded protein may play a role in regulating the availability of serum glycoproteins, oncogenesis, and differentiation. [provided by RefSeq, Jul 2008]