STAT1 (NM_007315) Human Untagged Clone

CAT#: SC115595

STAT1 (untagged)-Human signal transducer and activator of transcription 1, 91kDa (STAT1), transcript variant alpha

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STAT1
• Synonyms: CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_007315 edited
  CTGTGTATATAACCTCGACAGTCTTGGCACCTAACGTGCTGTGCGTAGCTGCTCCTTTGG
  TTGAATCCCCAGGCCCTTGTTGGGGCACAAGGTGGCAGGATGTCTCAGTGGTACGAACTT
  CAGCAGCTTGACTCAAAATTCCTGGAGCAGGTTCACCAGCTTTATGATGACAGTTTTCCC
  ATGGAAATCAGACAGTACCTGGCACAGTGGTTAGAAAAGCAAGACTGGGAGCACGCTGCC
  AATGATGTTTCATTTGCCACCATCCGTTTTCATGACCTCCTGTCACAGCTGGATGATCAA
  TATAGTCGCTTTTCTTTGGAGAATAACTTCTTGCTACAGCATAACATAAGGAAAAGCAAG
  CGTAATCTTCAGGATAATTTTCAGGAAGACCCAATCCAGATGTCTATGATCATTTACAGC
  TGTCTGAAGGAAGAAAGGAAAATTCTGGAAAACGCCCAGAGATTTAATCAGGCTCAGTCG
  GGGAATATTCAGAGCACAGTGATGTTAGACAAACAGAAAGAGCTTGACAGTAAAGTCAGA
  AATGTGAAGGACAAGGTTATGTGTATAGAGCATGAAATCAAGAGCCTGGAAGATTTACAA
  GATGAATATGACTTCAAATGCAAAACCTTGCAGAACAGAGAACACGAGACCAATGGTGTG
  GCAAAGAGTGATCAGAAACAAGAACAGCTGTTACTCAAGAAGATGTATTTAATGCTTGAC
  AATAAGAGAAAGGAAGTAGTTCACAAAATAATAGAGTTGCTGAATGTCACTGAACTTACC
  CAGAATGCCCTGATTAATGATGAACTAGTGGAGTGGAAGCGGAGACAGCAGAGCGCCTGT
  ATTGGGGGGCCGCCCAATGCTTGCTTGGATCAGCTGCAGAACTGGTTCACTATAGTTGCG
  GAGAGTCTGCAGCAAGTTCGGCAGCAGCTTAAAAAGTTGGAGGAATTGGAACAGAAATAC
  ACCTACGAACATGACCCTATCACAAAAAACAAACAAGTGTTATGGGACCGCACCTTCAGT
  CTTTTCCAGCAGCTCATTCAGAGCTCGTTTGTGGTGGAAAGACAGCCCTGCATGCCAACG
  CACCCTCAGAGGCCGCTGGTCTTGAAGACAGGGGTCCAGTTCACTGTGAAGTTGAGACTG
  TTGGTGAAATTGCAAGAGCTGAATTATAATTTGAAAGTCAAAGTCTTATTTGATAAAGAT
  GTGAATGAGAGAAATACAGTAAAAGGATTTAGGAAGTTCAACATTTTGGGCACGCACACA
  AAAGTGATGAACATGGAGGAGTCCACCAATGGCAGTCTGGCGGCTGAATTTCGGCACCTG
  CAATTGAAAGAACAGAAAAATGCTGGCACCAGAACGAATGAGGGTCCTCTCATCGTTACT
  GAAGAGCTTCACTCCCTTAGTTTTGAAACCCAATTGTGCCAGCCTGGTTTGGTAATTGAC
  CTCGAGACGACCTCTCTGCCCGTTGTGGTGATCTCCAACGTCAGCCAGCTCCCGAGCGGT
  TGGGCCTCCATCCTTTGGTACAACATGCTGGTGGCGGAACCCAGGAATCTGTCCTTCTTC
  CTGACTCCACCATGTGCACGATGGGCTCAGCTTTCAGAAGTGCTGAGTTGGCAGTTTTCT
  TCTGTCACCAAAAGAGGTCTCAATGTGGACCAGCTGAACATGTTGGGAGAGAAGCTTCTT
  GGTCCTAACGCCAGCCCCGATGGTCTCATTCCGTGGACGAGGTTTTGTAAGGAAAATATA
  AATGATAAAAATTTTCCCTTCTGGCTTTGGATTGAAAGCATCCTAGAACTCATTAAAAAA
  CACCTGCTCCCTCTCTGGAATGATGGGTGCATCATGGGCTTCATCAGCAAGGAGCGAGAG
  CGTGCCCTGTTGAAGGACCAGCAGCCGGGGACCTTCCTGCTGCGGTTCAGTGAGAGCTCC
  CGGGAAGGGGCCATCACATTCACATGGGTGGAGCGGTCCCAGAACGGAGGCGAACCTGAC
  TTCCATGCGGTTGAACCCTACACGAAGAAAGAACTTTCTGCTGTTACTTTCCCTGACATC
  ATTCGCAATTACAAAGTCATGGCTGCTGAGAATATTCCTGAGAATCCCCTGAAGTATCTG
  TATCCAAATATTGACAAAGACCATGCCTTTGGAAAGTATTACTCCAGGCCAAAGGAAGCA
  CCAGAGCCAATGGAACTTGATGGCCCTAAAGGAACTGGATATATCAAGACTGAGTTGATT
  TCTGTGTCTGAAGTTCACCCTTCTAGACTTCAGACCACAGACAACCTGCTCCCCATGTCT
  CCTGAGGAGTTTGACGAGGTGTCTCGGATAGTGGGCTCTGTAGAATTCGACAGTATGATG
  AACACAGTATAGAGCATGAATTTTTTTCATCTTCTCTGGCGACAGTTTTCCTTCTCATCT
  GTGATTCCCTCCTGCTACTCTGTTCCTTCACATCCTGTGTTTCTAGGGAAAT
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007315 unedited
  GTTCANATTTGTATCCGACTCCTATAGGCGGCCGCGCAATTCGCACGAGGGTTTCATTTG
  CTGTATGCCATCCTCGAGAGCTGTCTAGGTTAACGTTCGCACTCTGTGTATATAACCTCG
  ACAGTCTTGGCACCTAACGTGCTGTGCGTAGCTGCTCCTTTGGTTGAATCCCCAGGCCCT
  TGTTGGGGCACAAGGTGGCAGGATGTCTCAGTGGTACGAACTTCAGCAGCTTGACTCAAA
  ATTCCTGGAGCAGGTTCACCAGCTTTATGATGACAGTTTTCCCATGGAAATCAGACAGTA
  CCTGGCACAGTGGTTAGAAAAGCAAGACTGGGAGCACGCTGCCAATGATGTTTCATTTGC
  CACCATCCGTTTTCATGACCTCCTGTCACAGCTGGATGATCAATATAGTCGCTTTTCTTT
  GGAGAATAACTTCTTGCTACAGCATAACATAAGGAAAAGCAAGCGTAATCTTCAGGATAA
  TTTTCAGGAAGACCCAATCCAGATGTCTATGATCATTTACAGCTGTCTGAAGGAAGAAAG
  GAAAATTCTGGAAAACGCCCAGAGATTTAATCAGGCTCAGTCGGGGAATATTCAGAGCAC
  AGTGGATGTTAGACAAACAGAAAGAGCTTGACAGTAAAGTCAGAAATGTGAAGGACAAGG
  GTTATGTGTATAGAGCATGAAATCAAGAAGCCTGNAAGATTTACAAGATGAATATGACTT
  CAAATGCAAAACCTTGCAGAACAGAGAACACGAGACCANATGGTGTGGCCAAAGNAGTGA
  TCAGAAACAAGAACAGCTGTTACTCAAAAAGATGTATTTAATGCTTGACATAAGAGAAGG
  GAAGTAGTTCACAAATNAATAGAGTGCTGAATGTCACTGAACTTACCCAGATGCCCTGAT
  TATGATAACTANTGAAGTGGAAGCGAGACACAAACGCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007315 unedited
  ATGGCAACTCTCCAGGNCCAGGNATAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGG
  ATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTTTTTTTTGGATGCAGGGCCACGGAAAGCACTGGGGGCATA
  TTATATTTAATGCAATACAGATACTTTAACTTTAATTTTAAAACAAAACTCAAGAAATTT
  TTATCTCCACCAAAAAAAAATACAGCATTTGCATGAAAATATAGTTGGGGGAGCAGTAGG
  GGAAAAACAAGATACCGCCCCATAAACAGCAGTATGTAAAAGGGAAAAACCTTGGCAAAC
  CCATCTCTTAATCTACTTTTTGGGGCAGGGATAAGGAAGGAAAAAGGGAAAAATACAGTA
  TACTTCACGAAACATCATCCACTCAAAAAAGGGTCTGGAAAAAGTACAGGAGAGAGAATG
  GAACCCTTCGCAAATGGGAAAAACACATATATCAGCGAAACATATGCCGTTCTCAATGCA
  GTTACATAAGAAATGAGTTTTGAAAAGAACTGAATCTCATATTATCTCTGGGGGATTATT
  CAAGGTGGCAGGTACTGGTTTTTCTACTCCTTTCCCAATTTTGTGGGTAATAGACTAAAT
  ACCACCCTAATAACAAAAAGGACAAAGTAGCCCATTTAAGAAACATGAATTAAATTTCTG
  AGTTTATCTACATCTATGGGTAATTTCAACTTTNGGAATGAGGGGGTTGGGGAATAGCCC
  TAACTCCCCTCGGGAGGAACTGTAAAAATAATTTGGTTGTGACCAAGATGAAAGCAGAAT
  GTGTTTCAGCTTTGATATCAAACACCACTTGGGAATNGGGAAAAGNACATCCTTCTATTG
  GAATATAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007315
• Insert Size: 3750 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007315.2, NP_009330.1
• RefSeq Size: 4157 bp
• RefSeq ORF: 2253 bp
• Locus ID: 6772
• Cytogenetics: 2q32.2
• Domains: SH2, STAT
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Chemokine signaling pathway, Jak-STAT signaling pathway, Pancreatic cancer, Pathways in cancer, Toll-like receptor signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. The protein encoded by this gene can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. The protein plays an important role in immune responses to viral, fungal and mycobacterial pathogens. Mutations in this gene are associated with Immunodeficiency 31B, 31A, and 31C. [provided by RefSeq, Jun 2020]'
  Transcript Variant: This variant (alpha) encodes the longer protein (isoform alpha).