CD10 (MME) (NM_007287) Human Untagged Clone

CAT#: SC115606

MME (untagged)-Human membrane metallo-endopeptidase (MME), transcript variant 1bis

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MME
• Synonyms: CALLA; CD10; CMT2T; NEP; SCA43; SFE
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_007287, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCAAGTCAGAAAGTCAGATGGATATAACTGATATCAACACTCCAAAGCCAAAGAAGAAACAGCGAT
  GGACTCCACTGGAGATCAGCCTCTCGGTCCTTGTCCTGCTCCTCACCATCATAGCTGTGACAATGATCGC
  ACTCTATGCAACCTACGATGATGGTATTTGCAAGTCATCAGACTGCATAAAATCAGCTGCTCGACTGATC
  CAAAACATGGATGCCACCACTGAGCCTTGTACAGACTTTTTCAAATATGCTTGCGGAGGCTGGTTGAAAC
  GTAATGTCATTCCCGAGACCAGCTCCCGTTACGGCAACTTTGACATTTTAAGAGATGAACTAGAAGTCGT
  TTTGAAAGATGTCCTTCAAGAACCCAAAACTGAAGATATAGTAGCAGTGCAGAAAGCAAAAGCATTGTAC
  AGGTCTTGTATAAATGAATCTGCTATTGATAGCAGAGGTGGAGAACCTCTACTCAAACTGTTACCAGACA
  TATATGGGTGGCCAGTAGCAACAGAAAACTGGGAGCAAAAATATGGTGCTTCTTGGACAGCTGAAAAAGC
  TATTGCACAACTGAATTCTAAATATGGGAAAAAAGTCCTTATTAATTTGTTTGTTGGCACTGATGATAAG
  AATTCTGTGAATCATGTAATTCATATTGACCAACCTCGACTTGGCCTCCCTTCTAGAGATTACTATGAAT
  GCACTGGAATCTATAAAGAGGCTTGTACAGCATATGTGGATTTTATGATTTCTGTGGCCAGATTGATTCG
  TCAGGAAGAAAGATTGCCCATCGATGAAAACCAGCTTGCTTTGGAAATGAATAAAGTTATGGAATTGGAA
  AAAGAAATTGCCAATGCTACGGCTAAACCTGAAGATCGAAATGATCCAATGCTTCTGTATAACAAGATGA
  CATTGGCCCAGATCCAAAATAACTTTTCACTAGAGATCAATGGGAAGCCATTCAGCTGGTTGAATTTCAC
  AAATGAAATCATGTCAACTGTGAATATTAGTATTACAAATGAGGAAGATGTGGTTGTTTATGCTCCAGAA
  TATTTAACCAAACTTAAGCCCATTCTTACCAAATATTCTGCCAGAGATCTTCAAAATTTAATGTCCTGGA
  GATTCATAATGGATCTTGTAAGCAGCCTCAGCCGAACCTACAAGGAGTCCAGAAATGCTTTCCGCAAGGC
  CCTTTATGGTACAACCTCAGAAACAGCAACTTGGAGACGTTGTGCAAACTATGTCAATGGGAATATGGAA
  AATGCTGTGGGGAGGCTTTATGTGGAAGCAGCATTTGCTGGAGAGAGTAAACATGTGGTCGAGGATTTGA
  TTGCACAGATCCGAGAAGTTTTTATTCAGACTTTAGATGACCTCACTTGGATGGATGCCGAGACAAAAAA
  GAGAGCTGAAGAAAAGGCCTTAGCAATTAAAGAAAGGATCGGCTATCCTGATGACATTGTTTCAAATGAT
  AACAAACTGAATAATGAGTACCTCGAGTTGAACTACAAAGAAGATGAATACTTCGAGAACATAATTCAAA
  ATTTGAAATTCAGCCAAAGTAAACAACTGAAGAAGCTCCGAGAAAAGGTGGACAAAGATGAGTGGATAAG
  TGGAGCAGCTGTAGTCAATGCATTTTACTCTTCAGGAAGAAATCAGATAGTCTTCCCAGCCGGCATTCTG
  CAGCCCCCCTTCTTTAGTGCCCAGCAGTCCAACTCATTGAACTATGGGGGCATCGGCATGGTCATAGGAC
  ACGAAATCACCCATGGCTTCGATGACAATGGCAGAAACTTTAACAAAGATGGAGACCTCGTTGACTGGTG
  GACTCAACAGTCTGCAAGTAACTTTAAGGAGCAATCCCAGTGCATGGTGTATCAGTATGGAAACTTTTCC
  TGGGACCTGGCAGGTGGACAGCACCTTAATGGAATTAATACACTGGGAGAAAACATTGCTGATAATGGAG
  GTCTTGGTCAAGCATACAGAGCCTATCAGAATTATATTAAAAAGAATGGCGAAGAAAAATTACTTCCTGG
  ACTTGACCTAAATCACAAACAACTATTTTTCTTGAACTTTGCACAGGTGTGGTGTGGAACCTATAGGCCA
  GAGTATGCGGTTAACTCCATTAAAACAGATGTGCACAGTCCAGGCAATTTCAGGATTATTGGGACTTTGC
  AGAACTCTGCAGAGTTTTCAGAAGCCTTTCACTGCCGCAAGAATTCATACATGAATCCAGAAAAGAAGTG
  CCGGGTTTGGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007287 unedited
  NNGGTTCGTTTTTGTATACGACTCATATAGGGCGGCCGCGATTCGGCACGAGGGGCTCTG
  GAAGTCACGTCAGGTTGGCTCTTCAGGTTCATTTCCATAGTTCCCTGCGGCCTCTGCCTT
  GGGGAGTTATGTTTTGTTACCGAGATCCGCGCTACCAGATTGCACCGGGGCTGATTTGGG
  GGCTGGGAATTTGCCATTCTGCTGTACAGACACTGATTTTTTTTTCTTCTTTTTAAAAAG
  CAAGATTTTAGGTGATGGGCAAGTCAGAAAGTCAGATGGATATAACTGATATCAACACTC
  CAAAGCCAAAGAAGAAACAGCGATGGACTCCACTGGAGATCAGCCTCTCGGTCCTTGTCC
  TGCTCCTCACCATCATAGCTGTGACAATGATCGCACTCTATGCAACCTACGATGATGGTA
  TTTGCAAGTCATCAGACTGCATAAAATCAGCTGCTCGACTGATCCAAAACATGGATGCCA
  CCACTGAGCCTTGTACAGACTTTTTCAAATATGCTTGCGGAGGCTGGTTGAAACGTAATG
  TCATTCCCGAGACCAGCTCCCGTTACGGCAACTTTGACATTTTAAGAGATGAACTAGAAG
  TCGTTTTGAAAGATGTCCTTCAAGAACCCAAAACTGAAGATATAGTAGCAGTGCAGAAAG
  CAAAAGCATTGTACAGGTCTTGTATAAATGAATCTGCTATTGATAGCAGAGGTGGAGAAC
  CTCTACTCAAACTGTTACCAGACATATATGGGTGGCCAGTAGCAACAGAAAACTGGGAGC
  ANNAATATGGTGCTTCTTGGACAGCTGAANAAGCTATTGCACAACTGAATTNCTAATATG
  GGAAAAAGTCCTTATTAATTTGTTNGTTGGCACTGATGATAGAAATCTGTGAAATCATGT
  ATTCATATTGACCAACCTCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007287 unedited
  TTTTTTTTTTGGANCGCGGANCCGCATCTCAAGAGCCCAGTTTTTTTTTTTTTTTTGTTT
  ATATTAAAAACGTTTGTTTTTATTTTGTATTTTTGAGACTGGAAACTGATAAAAATTTCT
  GATTAGGAAACTTTGCAGAAAGAGCCTTTTTTCTTTCCAAAATAACAAATGTCTTTTAGT
  TTGAGAGCTGTATGTGTGGCAATAAGGATGATTTTTGCTTTTGATAAAATTAGTTGGCTT
  CGCCCCTCTTTTTCCGGCCCCTTTTTCATCTGTGCACCCTGGTTTTGTGTCGTCGTTTTT
  CCCGAGCTGCTGTCTGGCGCTTCAATTTTTCTTTTCTTTCCGATAATATTCCCCCCTTCT
  TCCCTTTTCCCCCTCTTTTTTTATTCCTCCCTGTGTTCGGCATCGCAGAAGCCGCCTCGT
  TTCCTTTTTCTTTCTGGGGTTTAGCTGCTCCTCCTCCGTTTCGTCTTTGTTGCGCTCCCG
  CCCCCCACCCCCCCTTATTCGACATCGGGTTGTTTGAGCACAGTGTTATGTTTGCTTTTT
  TTGCATCCGAATATTCCCAGTATTTTTTGTTAGCACATTTTTTTTTATCCCATTTTTATT
  CCTTTATGCCGCTCAACGTTCTTATATTTTTCTACGAGCATGCGTCTGTCATCTTTGAAT
  TTTACAGGTGTGTGTTATTTTTCCCTTTTATTGGCCCAGGTCGTAGTGAGGCGAGTAGCG
  TTTTTCCGCACCGTTCGTCGTACTTTTTTTTCGCGCTTCTATTAGGGCCTGCCTATTCGT
  GAGAGACGGATATATCCTTTACCTTCACACGCCCTTCTGTTTTTATCCTCCGCCCCTCGC
  TTTTTTTTGTTGTTTATTCGCCCCACCCCTGTGCTACCGATCTTTATTTTTGACTCACCC
  CCCATATTTTCTTTCTTTCGCCCCGCGCCTTTTATTTTGCTAGTTTTGAAACACCGGACC
  CCTTTTGTTTTCTCACGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007287
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007287.1, NP_009218.1
• RefSeq Size: 5665 bp
• RefSeq ORF: 2253 bp
• Locus ID: 4311
• Cytogenetics: 3q25.2
• Domains: Peptidase_M13
• Protein Families: Druggable Genome, Protease, Transmembrane
• Protein Pathways: Alzheimer's disease, Hematopoietic cell lineage, Renin-angiotensin system
• Gene Summary: 'The protein encoded by this gene is a type II transmembrane glycoprotein and a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). The encoded protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. [provided by RefSeq, Aug 2017]'
  Transcript Variant: This variant (1bis) contains an alternate 5' UTR exon compared to variant 2b. Variants 1, 1bis, 2a, 2b, 3, and 4 all encode the same isoform (a).