Mel18 (PCGF2) (NM_007144) Human Untagged Clone

CAT#: SC115690

PCGF2 (untagged)-Human polycomb group ring finger 2 (PCGF2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PCGF2
• Synonyms: MEL-18; RNF110; TPFS; ZNF144
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_007144, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCATCGGACTACACGGATCAAAATCACAGAGCTGAACCCCCACCTCATGTGTGCCCTCTGCGGGGGGT
  ACTTCATCGACGCCACCACTATCGTGGAGTGCCTGCATTCCTTCTGCAAAACCTGCATCGTGCGCTACCT
  GGAGACCAACAAATACTGCCCCATGTGTGACGTGCAGGTCCATAAAACCCGGCCGCTGCTGAGCATCAGG
  TCTGACAAAACACTTCAAGACATTGTCTACAAATTGGTCCCTGGGCTTTTTAAAGATGAGATGAAACGGC
  GGCGGGATTTCTATGCAGCGTACCCCCTGACGGAGGTCCCCAACGGCTCCAATGAGGACCGCGGCGAGGT
  CTTGGAGCAGGAGAAGGGGGCTCTGAGTGATGATGAGATTGTCAGCCTCTCCATCGAATTCTACGAAGGT
  GCCAGGGACCGGGACGAGAAGAAGGGCCCCCTGGAGAATGGGGATGGGGACAAAGAGAAAACAGGGGTGC
  GCTTCCTGCGATGCCCAGCAGCCATGACCGTCATGCATCTTGCCAAGTTTCTCCGCAACAAGATGGATGT
  GCCCAGCAAGTACAAGGTGGAGGTTCTGTACGAGGACGAGCCACTGAAGGAATACTACACCCTCATGGAC
  ATCGCCTACATCTACCCCTGGCGGCGGAACGGGCCTCTCCCCCTCAAGTACCGTGTCCAGCCAGCCTGCA
  AGCGGCTCACCCTAGCCACGGTGCCCACCCCCTCCGAGGGCACCAACACCAGCGGGGCGTCCGAGTGTGA
  GTCAGTCAGCGACAAGGCTCCCAGCCCTGCCACCCTGCCAGCCACCTCCTCCTCCCTGCCCAGCCCAGCC
  ACCCCATCCCATGGCTCTCCCAGTTCCCATGGGCCTCCAGCCACCCACCCTACCTCCCCCACTCCCCCTT
  CGACAGCCAGTGGGGCCACCACAGCTGCCAACGGGGGTAGCTTGAACTGCCTGCAGACACCATCCTCCAC
  CAGCAGGGGGCGCAAGATGACTGTCAACGGCGCTCCCGTGCCCCCCTTAACTTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007144 unedited
  TCACAAATTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCTCCCCG
  TCTTAACGATTTGGCGTCTCCCTCGACCACCACCTCTTTGTGCAGCAGCCCCCGGGCAGA
  CCCTGTTCCGAGGGCAACGCTCCCCAGTCCCCCCACCCCCGACCCCGGAATCATGCATCG
  GACTACACGGATCAAAATCACAGAGCTGAACCCCCACCTCATGTGTGCCCTCTGCGGGGG
  GTACTTCATCGACGCCACCACTATCGTGGAGTGCCTGCATTCCTTCTGCAAAACCTGCAT
  CGTGCGCTACCTGGAGACCAACAAATACTGCCCCATGTGTGACGTGCAGGTCCATAAAAC
  CCGGCCGCTGCTGAGCATCAGGTCTGACAAAACACTTCAAGACATTGTCTACAAATTGGT
  CCCTGGGCTTTTTAAAGATGAGATGAAACGGCGGCGGGATTTCTATGCAGCGTACCCCCT
  GACGGAGGTCCCCAACGGCTCCAATGAGGACCGCGGCGAGGTCTTGGAGCAGGAGAAGGG
  GGCTCTGAGTGATGATGAGATTGTCAGCCTCTCCATCGAATTCTACGAAGGTGCCAGGGA
  CCGGGACGAGAAGAAGGGCCCCCTGGAGAATGGGGATGGGGACAAAGAGAAAACAGGGGT
  GCGCTTCCTGCGATGCCCAGCAGCCATGACCGTCATGCATCTTGCCAAGTTTCTCCGCAA
  CAAGATGGATGTGCCCAGCAAGTACAAGGTGGAGGGTTCTGTACGAAGACGAGCCACTGA
  AGGAATACTACACCCTCATGGACATCGCCTACATCTACCCCTGGCGGCGGAACGGGCCTC
  TNCCCCTCAAGTACGTGTNCAGCCAGCCTGCAAGCGGCTCACCCTAGCCACGGTGCCCAC
  CCCCTNCGAGGGCACCCACACAGCCGGNGCGTCCGANTGTTGAGTCAGTCAGCGACAAGG
  CTCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007144 unedited
  GCTTATATATATATATTTATTTATAAAACCCGCCCCCCCCCCCAGGTGGGAAGAGCTGGG
  GAAAGTGAAGAGTGGAAAAAGGGCCCAGAAAAGTGGAAGGAGTGGAGAGGCTTGGCTGGA
  AGAAGGGAGAGGGTCCCTGGCCTCAAGTTAAGGGGGGCACGGGAGCGCCGTTGACAGTCA
  TCTTGCGCCCCCTGCTGGTGGAGGATGGTGTCTGCAGGCAGTTCAAGCTACCCCCGTTGG
  CAGCTGTGGTGGCCCCACTGGCTGTCGAAGGGGGAGTGGGGGAGGTAGGGTGGGTGGCTG
  GAGGCCCATGGGAACTGGGAGAGCCATGGGATGGGGTGGCTGGGCTGGGCAGGGAGGAGG
  ACGTGGCTGGCAGGGTGGCAGGGCTGGGAGCCTTGTCGCTGACTGACTTACACTCGGACG
  CCCCGCTGGTGTTGGTGCCCTCGGAGGGGGTGGGCACCGTGGCTAGGGTGAGCCGCTTGC
  AGGCTGGCTGGACACGGTCCTCGAGGGGGAGAGGCCCGTTCCGCCCCCAGGGGTACATTN
  ACGCCATGTCCATGATGGCGTCGCATTCCTTCAGTGGCTCGTCCTCCTACAGAACCTCCA
  CCTTGCACTTGCCGCGCACACCCTCTTTGTTGCGGGAAACTCGTCAAGACGCATTACTGT
  CATGGCTGCTGCGCATCTCAGGAAACGTCACCCTGTTCTTTTTTTGTACCCATCCCCATT
  TCTCAAGGGGGCTCTTTTTTTCCGTCCGCGGCCTTGCACCCTCTCCTCAAATCCGCTCGA
  AAGCTTTCCACCTTCTACTCCCCCAAAACCCCCTTCCTTTCGTTTATCACCCCCCCCCGT
  CTCTATTTTGATTCTTTGGCACCCCACCATGCGCTCCCTTCTCAAATCCTCCCCTTCTCC
  TCCTTCTGTTTAAACCCCGCCCATTNTGTGCCCATGTTCAGATTTCTCNATACTCTCTTT
  TTCCGCTCCTTTTTCTGCCTATCGTCACTAGATCCTTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007144
• Insert Size: 1440 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007144.2, NP_009075.1
• RefSeq Size: 2622 bp
• RefSeq ORF: 1035 bp
• Locus ID: 7703
• Cytogenetics: 17q12
• Domains: RING
• Protein Families: Transcription Factors
• Gene Summary: 'The protein encoded by this gene contains a RING finger motif and is similar to the polycomb group (PcG) gene products. PcG gene products form complexes via protein-protein interaction and maintain the transcription repression of genes involved in embryogenesis, cell cycles, and tumorigenesis. This protein was shown to act as a negative regulator of transcription and has tumor suppressor activity. The expression of this gene was detected in various tumor cells, but is limited in neural organs in normal tissues. Knockout studies in mice suggested that this protein may negatively regulate the expression of different cytokines, chemokines, and chemokine receptors, and thus plays an important role in lymphocyte differentiation and migration, as well as in immune responses. [provided by RefSeq, Jul 2008]'