ATP5ME (NM_007100) Human Untagged Clone

CAT#: SC115719

ATP5I (untagged)-Human ATP synthase, H+ transporting, mitochondrial Fo complex, subunit E (ATP5I), nuclear gene encoding mitochondrial protein, transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP5ME
• Synonyms: ATP5I; ATP5K
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115719 sequence for NM_007100 edited (data generated by NextGen Sequencing)
  ATGGTGCCACCGGTGCAGGTCTCTCCGCTCATCAAGCTCGGCCGCTACTCCGCCCTGTTC
  CTCGGTGTGGCCTACGGAGCCACGCGCTACAATTACCTAAAACCTCGGGCAGAAGAGGAG
  AGGAGGATAGCAGCAGAAGAGAAGAAGAAGCAGGATGAACTGAAACGGATTGCCAGAGAA
  TTGGCAGAAGATGACAGCATATTAAAGTGA
  
  Clone variation with respect to NM_007100.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007100 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCTCCAAGGCTTTGCTGG
  CTTGTGCGGCATCCTGCTCCGTCTGCAGGTTGTGCTTCCGGTGCGGAGGTCAGGGACAAG
  ATGGTGCCACCGGTGCAGGTCTCTCCGCTCATCAAGCTCGGCCGCTACTCCGCCCTGTTC
  CTCGGTGTGGCCTACGGAGCCACGCGCTACAATTACCTAAAACCTCGGGCAGAAGAGGAG
  AGGAGGATAGCAGCAGAAGAGAAGAAGAAGCAGGATGAACTGAAACGGATTGCCAGAGAA
  TTGGCAGAAGATGACAGCATATTAAAGTGAGTGACCCTGCGACCCACTCTTTGGACCAGC
  AGCGGATGAATAAAGCTTCCTGTGTTGTGTGAAAAAAAAAAAAAAAAAACTCGACTCTAG
  ATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCC
  TCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTA
  ATAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATATATTATGGGGTGGAG
  GGGGGTGGTACTGACCCAGGGGCAAGTTGGGAAGACAACCTGTAGGGCCTGCGGGCTCTA
  TTGGAACCAACCTGGAGTGCAGTGGCACAATCNTGCCTCACTGCAATCTCCCGCTCCCTG
  GGTCAAGCGATTCTCCTTGCTCACCCTCCCGAGTTGTTTGGAATTTCCAGCTTGCCTGAC
  CAGGCTCAGCTAATTTTTGGTTTTTTTGTAAGAGACCGGGTNTTAACCATATTTGCCAGG
  CTTGTTCCCCAACTCCCTATCTCAGGTGATTTACCCCACTTGGCCCTCCCAATTGGCTGG
  ATTACGGCCTGGAACCCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007100 unedited
  NTTACTTTGAACCCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTAACACA
  ACACAGGAAGCTTTATTCATCCGCTGCTGGTCCAAAAATGGGTCGCAGGGTCACTCACTT
  TAATATGCTGTCATCTTCTGCCAATTCTCTGGCAATCCGTTTCAGTTCATCCTGCTTCTT
  CTTCTCTTCTGCTGCTATCCTCCTCTCCTCTTCTGCCCGAGGTTTTAGGTAATTGTAGCG
  CGTGGCTCCGTAGGCCACACCGAGGAACAGGGCGGAGTAGCGGCCGAGCTTGATGAGCGG
  AGAGACCTGCACCGGTGGCACCATCTTGTCCCTGACCTCCGCACCGGAAGCACAACCTGC
  AGACGGAGCAGGATGCCGCACAAGCCAGCAAAGCCTTGGAGCCTCGTGCCGAATTCGCGG
  CCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACGAGCTCTGCTTAT
  ATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCAACGGGGCGGGGTTATTACG
  ACATTTTGGAAAGTCCCGTTGATTTTGGTGCCAAAACAAACTCCCATTGACGTCAATGGG
  GTGGAGACTTGGAAATCCCCGTGAGTCAAACCGCTATCCACGCCCATTGGTGTACTGCCA
  AAACCGCATCACCATGGTAATAGCGATGACTAATACCTAGATGTACTGCCAAGTANGAAA
  GTCCCGTAAGGTCATGTACTGGGCATAATGCCAGGCGGGCCATTTACCGTCATTGACGTC
  AATAGGGGGCGAACTTGGCTATGATACACTTGATGTACTGCCAAGTGGGCAGTTTACCGT
  AAATACTCACCCATTGACGTCAATGGAAAGTCCCTATGGCGTTACTATGGAACCTACCTC
  ATTATTGACTCAATGGCCCNGGGGTCTTTGGCGGTTAACCCAGCGGCCCTTTACCGTAGT
  TATGTACCCCGCAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007100
• Insert Size: 380 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007100.2, NP_009031.1
• RefSeq Size: 368 bp
• RefSeq ORF: 210 bp
• Locus ID: 521
• Cytogenetics: 4p16.3
• Protein Pathways: Metabolic pathways, Oxidative phosphorylation
• Gene Summary: 'Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the e subunit of the Fo complex. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jun 2010]'
  Transcript Variant: This variant (1) represents the longer transcript.