RPL10A (NM_007104) Human Untagged Clone

CAT#: SC115722

RPL10A (untagged)-Human ribosomal protein L10a (RPL10A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPL10A
• Synonyms: Csa-19; CSA19; L10A; NEDD6
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115722 sequence for NM_007104 edited (data generated by NextGen Sequencing)
  ATGAGCAGCAAAGTCTCTCGCGACACCCTGTACGAGGCGGTGCGGGAAGTCCTGCACGGG
  AACCAGCGCAAGCGCCGCAAGTTCCTGGAGACGGTGGAGTTGCAGATCAGCTTGAAGAAC
  TATGATCCCCAGAAGGACAAGCGCTTCTCGGGCACCGTCAGGCTTAAGTCCACTCCCCGC
  CCTAAGTTCTCTGTGTGTGTCCTGGGGGACCAGCAGCACTGTGACGAGGCTAAGGCCGTG
  GATATCCCCCACATGGACATCGAGGCGCTGAAAAAACTCAACAAGAATAAAAAACTGGTC
  AAGAAGCTGGCCAAGAAGTATGATGCGTTTTTGGCCTCAGAGTCTCTGATCAAGCAGATT
  CCACGAATCCTCGGCCCAGGTTTAAATAAGGCAGGAAAGTTCCCTTCCCTGCTCACACAC
  AACGAAAACATGGTGGCCAAAGTGGATGAGGTGAAGTCCACAATCAAGTTCCAAATGAAG
  AAGGTGTTATGTCTGGCTGTAGCTGTTGGTCACGTGAAGATGACAGACGATGAGCTTGTG
  TATAACATTCACCTGGCTGTCAACTTCTTGGTGTCATTGCTCAAGAAAAACTGGCAGAAT
  GTCCGGGCCTTATATATCAAGAGCACCATGGGCAAGCCCCAGCGCCTATATTAA
  
  Clone variation with respect to NM_007104.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_007104 unedited
  GCACGAGGGGCGTGAGAAGCCATGAGCAGCAAAGTCTCTCGCGACACCCTGTACGAGGCG
  GTGCGGGAAGTCCTGCACGGGAACCAGCGCAAGCGCCGCAAGTTCCTGGAGACGGTGGAG
  TTGCAGATCAGCTTGAAGAACTATGATCCCCAGAAGGACAAGCGCTTCTCGGGCACCGTC
  AGGCTTAAGTCCACTCCCCGCCCTAAGTTCTCTGTGTGTGTCCTGGGGGACCAGCAGCAC
  TGTGACGAGGCTAAGGCCGTGGATATCCCCCACATGGACATCGAGGCGCTGAAAAAACTC
  AACAAGAATAAAAAACTGGTCAAGAAGCTGGCCAAGAAGTATGATGCGTTTTTGGCCTCA
  GAGTCTCTGATCAAGCAGATTCCACGAATCCTCGGCCCAGGTTTAAATAAGGCAGGAAAG
  TTCCCTTCCCTGCTCACACACAACGAAAACATGGTGGCCAAAGTGGATGAGGTGAAGTCC
  ACAATCAAGTTCCAAATGAAGAAGGTGTTATGTCTGGCTGTAGCTGTTGGTCACGTGAAG
  ATGACAGACGATGAGCTTGTGTATAACATTCACCTGGCTGTCAACTTCTTGGTGTCATTG
  CTCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_007104 unedited
  CGCGCCGCTATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAACTGGTAATAGAATTTATT
  CAAATGTGCCTTAATATAGGCGCTGGGGCTTGCCCATGGTGCTCTTGATATATAAGGCCC
  GGACATTCTGCCAGTTTTTCTTGAGCAATGACACCAAGAAGTTGACAGCCAGGTGAATGT
  TATACACAAGCTCATCGTCTGTCATCTTCACGTGACCAACAGCTACAGCCAGACATAACA
  CCTTCTTCATTTGGAACTTGATTGTGGACTTCACCTCATCCACTTTGGCCACCATGTTTT
  CGTTGTGTGTGAGCAGGGAAGGGAACTTTCCTGCCTTATTTAAACCTGGGCCGAGGATTC
  GTGGAATCTGCTTGATCAGAGACTCTGAGGCCAAAAACGCATCATACTTCTTGGCCAGCT
  TCTTGACCAGTTTTTTATTCTTGTTGAGTTTTTTCAGCGCCTCGATGTCCATGTGGGGGA
  TATCCACGGCCTTAGCCTCGTCACAGTGCTGCTGGTCCCCCAGGACACACACAGAGAACT
  TAGGGCGGGGAGTGGACTTAAGCCTGACGGTGCCCGAGAAGCGCTTGTCCTTCTGGGGAT
  CATAGTTCTTCAAGCTGATCTGCAACTCCACCGTCTCCAGGAACTTGCGGCGCTTGCGCT
  GGTTCCCGTGCAGGACTTTCCGCACCGCCTTGTAAGGGTGTCGCGAGAACTTTGCTGCTC
  ATGCTTCTAAACCCCCTGTGCCGAATTCCCGGCCGCCCATAGCGAGTCCTTTACCAAAAT
  CTGCACGTCCCTAAACGAACCTTGTTATATAACCTCCACCGACACCCTCCCGGCATTTGC
  GTAACGGGCGGGGTTTAGAAACTTTGAAACCCCGTGATTTGGGCCAAAAACTCCTTGCCC
  AAGGGTGGCACGGAATCCCCGATCAACCCTCCCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_007104
• Insert Size: 760 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007104.4, NP_009035.3
• RefSeq Size: 719 bp
• RefSeq ORF: 654 bp
• Locus ID: 4736
• Cytogenetics: 6p21.31
• Domains: Ribosomal_L1
• Protein Pathways: Ribosome
• Gene Summary: 'Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L1P family of ribosomal proteins. It is located in the cytoplasm. The expression of this gene is downregulated in the thymus by cyclosporin-A (CsA), an immunosuppressive drug. Studies in mice have shown that the expression of the ribosomal protein L10a gene is downregulated in neural precursor cells during development. This gene previously was referred to as NEDD6 (neural precursor cell expressed, developmentally downregulated 6), but it has been renamed RPL10A (ribosomal protein 10a). As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]'