SC5DL (SC5D) (NM_006918) Human Untagged Clone

CAT#: SC115783

SC5DL (untagged)-Human sterol-C5-desaturase (ERG3 delta-5-desaturase homolog, S. cerevisiae)-like (SC5DL), transcript variant 1


  "NM_006918" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol SC5D
Synonyms ERG3; S5DES; SC5DL
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC115783 sequence for NM_006918 edited (data generated by NextGen Sequencing)
ATGGATCTTGTACTCCGTGTTGCAGATTACTATTTTTTTACACCATACGTGTATCCAGCC
ACATGGCCAGAAGATGACATCTTCCGACAAGCTATTAGTCTTCTGATTGTAACAAATGTT
GGTGCTTACATCCTTTATTTCTTCTGTGCAACACTGAGCTATTATTTTGTCTTCGATCAT
GCATTAATGAAACATCCACAATTTTTAAAGAATCAAGTCCGTCGAGAGATTAAGTTTACT
GTCCAGGCATTGCCATGGATAAGTATTCTTACTGTTGCACTGTTCTTGCTGGAGATAAGA
GGTTACAGCAAATTACATGATGACCTAGGAGAGTTTCCATATGGATTGTTTGAACTTGTC
GTTAGTATAATATCTTTCCTCTTTTTCACTGACATGTTCATCTACTGGATTCACAGAGGC
CTTCATCATAGACTGGTATATAAGCGCCTACATAAACCTCACCATATTTGGAAGATTCCT
ACTCCATTTGCAAGTCATGCTTTTCACCCTATTGATGGCTTTCTTCAGAGTCTACCTTAC
CATATATACCCTTTTATCTTTCCATTACACAAGGTGGTTTATTTAAGTCTGTACATCTTG
GTTAATATCTGGACAATTTCCATTCATGACGGTGATTTTCGTGTCCCCCAAATCTTACAG
CCATTTATTAATGGCTCAGCTCATCATACAGACCACCATATGTTCTTTGACTATAATTAT
GGACAATATTTCACTTTGTGGGATAGGATTGGCGGCTCATTCAAAAATCCTTCATCCTTT
GAGGGGAAGGGACCGCTCAGTTATGTGAAGGAGATGACAGAGGGAAAGCGCAGCAGCCAT
TCAGGAAATGGCTGTAAGAATGAAAAATTATTCAATGGAGAGTTTACAAAGACTGAATAG

Clone variation with respect to NM_006918.4
>OriGene 5' read for NM_006918 unedited
GTTTGGATTTTGTAATCCGACTTACTATAGGGNCGGCACGCGCAATTCGGCACGAGGGGC
GTGCGGAGCGGCGGCGGACCACCTCCAGGGCTAAGTGATGGATCTTGTACTCCGTGTTGC
AGATTACTATTTTTTTACACCATACGTGTATCCAGCCACATGGCCAGAAGATGACATCTT
CCGACAAGCTATTAGTCTTCTGATTGTAACAAATGTTGGTGCTTACATCCTTTATTTCTT
CTGTGCAACACTGAGCTATTATTTTGTCTTCGATCATGCATTAATGAAACATCCACAATT
TTTAAAGAATCAAGTCCGTCGAGAGATTAAGTTTACTGTCCAGGCATTGCCATGGATAAG
TATTCTTACTGTTGCACTGTTCTTGCTGGAGATAAGAGGTTACAGCAAATTACATGATGA
CCTAGGAGAGTTTCCATATGGATTGTTTGAACTTGTCGTTAGTATAATATCTTTCCTCTT
TTTCACTGACATGTTCATCTACTGGATTCACAGAGGCCTTCATCATAGACTGGTATATAA
GCGCCTACATAAACCTCACCATATTTGGAAGATTCCTACTCCATTTGCAAGTCATGCTTT
TCACCCTATTGATGGCTTTCTTCAGAGTCTACCTTACCATATATACCCTTTTATCTTTCC
ATTACACAAGGTGGTTTATTTAAGTCTGTACATCTTGGTTAATATCTGGACAATTTCCAT
TCATGACGGTGNATTTCGTGTCCCCCATATCTTACAGCCATTTATTAATGGCTCAGCTCA
TCATACAGACCACCATATGTTCTTTGACTATNATTATGGNACATATTTCACTTTGTGGGG
ATAGATGGNNCGCTCATNCAAAATCCNTCATCCTTNGAGGAGAAGGNACCGCTCANNTAT
GTGAAAGAGATGACAGAGGGAATGCGCAGCACCATTCAGGAATGGCTGTAGAATGAAAAC
TAT
>OriGene 3' read for NM_006918 unedited
CGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTTAATTATGATCAACTT
TTATTGATTTTACATAAATAGCTTATAGAATGCCTTTACAAACTATGTTTTTGCAATATA
TTTGTTACAACACACATTTCCTTATGAATGTATTTGTTAAATCTTTTTGCAGAGGAAGTA
GGGAAAAATGAGGGGAGAAGAGGAGGAATAAGATTTTAGAACTAATCGTCAGCAATGGGC
CTAGGTATTTGGCAATTATTTCCTTTACAAAAAAATTACAGGTTTGTAACTTCAAACATC
AAAAGTGTAGTGCATCAATTTAACTAAAAAATCAGTAGTGCCTAATATATTATCTGGGCA
TCAAATTTCTTTTTTTAAATATATCCAGATTCACATATTTTTACTCTTATTAAATAATGG
TTTTAAATAATAATCATCACAATGTTTAGCTACATAATCCCAGATCTGGATTAAATCATG
GTGATATCGATATTTTTAAATTCCAATTTATTTGTTAATCAGTAAAATGTAATAGGTGAT
AGTGTGTCCAGAGAATGAGCCTCCTATGTGTAGGAAGACGATAGTCTTATTACACCTGCT
ATCAATTAGGTCATTTACATAGCTGCTAATGTATCAGAATTTTTTTCCACACTGATGTAG
TACTTTATTCAAGTCAAAACAAGTTATCATTATCATACCAACGTTGAAGAATTAACCATT
CCTGAAACAAGAAACAATCTTTACATTAGTATTTCTTGGTTATGGGGCTTAGTCTTGGTA
TTATTAAGCACAATGATTCTTAATAAACATGGNGTGGATGANAGGCAATGCATATAGCCT
ANTCCCCCTCATATTTATTCTNCATCTTATTTAAAATGNTGNTGAGGGCTATACAACTAC
AGATCTGCTGCTAGAAAGAACAAATTGTCCCCGGACACATCTGCATTTTAGCATAGATTA
GNNCTGGNGTCCAGN
Restriction Sites NotI-NotI     
ACCN NM_006918
Insert Size 2300 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006918.3, NP_008849.2
RefSeq Size 2164 bp
RefSeq ORF 900 bp
Locus ID 6309
Cytogenetics 11q23.3-q24.1
Domains Sterol_desat
Protein Families Druggable Genome, Transmembrane
Protein Pathways Metabolic pathways, Steroid biosynthesis
Gene Summary 'This gene encodes an enzyme of cholesterol biosynthesis. The encoded protein catalyzes the conversion of lathosterol into 7-dehydrocholesterol. Mutations in this gene have been associated with lathosterolosis. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (1) represents the shorter transcript. Variants 1 and 2 encode the same protein.

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