EIF2D (NM_006893) Human Untagged Clone

CAT#: SC115805

EIF2D (untagged)-Human eukaryotic translation initiation factor 2D (EIF2D), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EIF2D
• Synonyms: HCA56; LGTN
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115805 sequence for NM_006893 edited (data generated by NextGen Sequencing)
  ATGTTTGCCAAGGCCTTTCGGGTCAAGTCCAACACGGCCATCAAGGGGTCGGACAGGAGA
  AAGCTTCGAGCTGATGTGACAACTGCTTTCCCCACCCTTGGAACTGATCAAGTCTCTGAG
  TTAGTACCTGGAAAGGAGGAGCTCAACATTGTGAAGTTGTATGCTCACAAAGGGGATGCA
  GTGACTGTGTACGTGAGTGGTGGTAACCCCATCCTCTTTGAACTGGAGAAAAATCTGTAT
  CCAACAGTGTACACGCTGTGGTCCTATCCTGATCTTCTGCCAACCTTTACAACATGGCCT
  CTGGTGCTCGAGAAACTGGTAGGGGGAGCAGATTTGATGCTGCCTGGACTGGTGATGCCC
  CCTGCTGGTCTGCCTCAGGTACAGAAGGGCGACCTCTGTGCCATTTCTTTGGTGGGGAAC
  AGAGCCCCTGTAGCCATTGGAGTTGCAGCCATGTCCACAGCTGAGATGCTCACGTCAGGC
  CTGAAGGGAAGGGGCTTCTCTGTGCTCCACACTTACCAGGACCACTTGTGGCGGTCTGGA
  AACAAGTCCTCTCCACCTTCCATTGCTCCACTGGCCCTGGATTCAGCAGATCTCAGTGAA
  GAGAAGGGGTCTGTCCAGATGGACTCCACCCTGCAGGGAGACATGAGGCACATGACCCTG
  GAGGGGGAAGAGGAGAATGGGGAGGTTCACCAGGCACGTGAAGACAAGTCTCTCTCAGAA
  GCCCCAGAAGACACCAGCACCAGGGGCCTGAACCAAGACTCCACAGATAGCAAAACGCTT
  CAAGAACAAATGGATGAGCTGTTACAGCAATGCTTCTTACATGCCTTGAAGTGCCGAGTC
  AAAAAGGCTGACCTCCCTTTACTCACCAGCACTTTCCTTGGCAGCCACATGTTCTCCTGC
  TGCCCCGAAGGACGACAACTGGACATAAAGAAGTCAAGCTACAAAAAGCTCTCTAAGTTC
  CTGCAGCAAATGCAGCAGGAGCAGATTATACAGGTGAAGGAGCTGAGCAAAGGGGTGGAG
  AGCATTGTGGCTGTGGACTGGAAACACCCGAGGATTACATCTTTCGTCATACCCGAGCCC
  TCCCCGACCTCCCAGACTATCCAGGAGGGTAGCAGGGAACAGCCCTATCACCCTCCAGAT
  ATAAAACCCCTCTACTGTGTCCCAGCCAGCATGACCCTGCTCTTCCAGGAGTCTGGCCAC
  AAGAAGGGGAGCTTTCTGGAGGGCAGTGAGGTCCGAACGATCGTCATTAACTACGCCAAG
  AAAAATGACCTGGTTGATGCAGACAACAAAAATCTTGTGAGATTGGATCCCATCCTATGT
  GACTGCATCTTAGAGAAAAATGAACAGCATACAGTCATGAAGCTTCCATGGGACAGTCTT
  CTGACCAGGTGTTTGGAAAAATTACAGCCTGCCTATCAAGTGACCCTTCCCGGACAAGAG
  CCCATTGTGAAGAAAGGGAGAATCTGTCCAATTGACATCACCCTAGCACAAAGAGCGTCT
  AATAAAAAGGTGACCGTGGTCCGGAACTTGGAGGCCTATGGTCTGGACCCATACTCAGTG
  GCTGCCATCCTTCAGCAGCGATGCCAGGCTAGCACCACCGTCAATCCTGCCCCTGGGGCC
  AAGGACAGCCTTCAGGTGCAGATCCAGGGAAACCAGGTCCACCACCTCGGCTGGCTATTG
  CTTGAAGAGTATCAGCTCCCTCGAAAACACATCCAAGGTCTAGAAAAGGCCCTCAAACCT
  GGCAAGAAGAAGTGA
  
  Clone variation with respect to NM_006893.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006893 unedited
  GGTTTTGCCGGNATTTTGGTATACGTACTCACTATAGGGCGGCCGCGATTCGGCACGAGC
  CACGGCTGAGGGCCTGGCAGCTGCTGCGCCCTCGCTTTCTTGACATTCCCTGGCTTCTGT
  GCTCTCTTCCCCAGGCCACCCCAGCAGACATGTTTGCCAAGGCCTTTCGGGTCAAGTCCA
  ACACGGCCATCAAGGGGTCGGACAGGAGAAAGCTTCGAGCTGATGTGACAACTGCTTTCC
  CCACCCTTGCAACTGATCAAGTCTCTGAGTTAGTACCTGGAAAGGAGGAGCTCACCATTG
  TGAAGTTGTATGCTCACAAAGGGGATGCAGTGACTGTGTACGTGAGTGGTGGTAACCCCA
  TCCTCTTTGAACTGGAGAAAAATCTGTATCCAACAGTGTACACGCTGTGGTCCTATCCTG
  ATCTTCTGCCAACCTTTACAACATGGCCTCTGGTGCTCGAGAAACTGGTAGGGGGAGCAG
  ATTTGATGCTGCCTGGACTGGTGATGCCCCCTGCTGGTCTGCCTCAGGTACAGAAGGGCG
  ACCTCTGTGCCATTTCTTTGGTGGGGAACAGAGCCCCTGTAGCCATTGGAGTTGCAGCCA
  TGTCCACAGCTGATATGCTCACGTCAGGCCTGAAGGGAAGGGGCTTCTCTGTGCTCCACA
  CTTACCAGGACCACTTGTGCCGGTCTGGAAACAGTCCTCTCCACCTTCATTGCTCCACTG
  GCCCTGGATCAGCAGATCTCAGTGAAGAGAAGGGTCTGTCCAGATGTACTCCACCTGCAG
  GGAGACATGAGGCCCATGACCCTGCAGGGCGAACAGAAATGGGTGAAGTCTCACCAGCAC
  GTGAAAACAAGTCTCTCTAAAAGCCCCCAAGACACAGCAACCAGGGCCTGAACCAGATTC
  CCAAAAACAAAAGCTTCAGACCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006893 unedited
  NTCAGCTTGGNACCGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTTAGATT
  AGAATAAAAATTTATTTTTGTAAAGAATTATATTTTGTATTTGCAAAAGCTGAAAATGCT
  CATAAAAATTACCAGCCCAGAGCTTGGATTTCCACCGGATCCACCACGTGAGACAAAAGA
  GTCTGTCACTTCTTCTTGCCAGGTTTGAGGGCCTTTTCTAGACCTTGGATGTGTTTTCGA
  GGGAGCTGATACTCTTCAAGCAATAGCCAGCCGAGGTGGTGGACCTGGTTTCCCTGGATC
  TGCACCTGAAGGCTGTCCTTGGCCCCAGGGGCAGGATTGACGGTGGTGCTAGCCTGGCAT
  CGCTGCTGAAGGATGGCAGCCACTGAGTATGGGTCCAGACCATAGGCCTCCAAGTTCCGG
  ACCACGGTCACCTTTTTATTAGACGCTCTTTGTGCTAGGGTGATGTCAATTGGACAGATT
  CTCCCTTTCTTCACAATGGGCTCTTGTCCGGGAAGGGTCACTTGATAGGCAGGCTGTAAT
  TTTTCCAAACACCTGGTCAGAAGACTGTCCCATGGAAGCTTCATGACTGTATGCTGTTCA
  TTNTTCTCTAAGATGCAGTCACATAGGATGGGATCCAATCTCACAAGATTTTTGTTGTCT
  GCATCAACCAGGTCATTTTTCTTGGCGTAAGTAATGACGATCGTTCGGACCTCACTGCCC
  TCCAAAAGCCTCCCTTTCTGGTGGCCAGAACTCCTGAAAGAGCAGGGGCATGCCTGGCTG
  GGACACCAGTAGAAGGGGTTTATAATCTGGAAGGTGGAAAAGGGCGTGTCCCTGGTACCC
  TCCTGGAATACCTGGGGGGGCCGGGGAGGGCTCCGGTATGACAAAAGAAGAAATCCTCGG
  GGGTTCCAGTCCACAGCCCTTGGCTCTCCCACTCTTGCGTACTCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006893
• Insert Size: 2050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006893.2, NP_008824.2
• RefSeq Size: 2115 bp
• RefSeq ORF: 1755 bp
• Locus ID: 1939
• Cytogenetics: 1q32.1
• Domains: SUI1, PUA
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a translation initiation factor involved in the recruitment and delivery of aminoacyl-tRNAs to the P-site of the eukaryotic ribosome in a GTP-independent manner. This gene was previously referred to as ligatin, but is now known to localize to the cytoplasm and localize and function with translation factors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).