EIF2D (NM_006893) Human Untagged Clone

CAT#: SC322496

EIF2D (untagged)-Human eukaryotic translation initiation factor 2D (EIF2D), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EIF2D
• Synonyms: HCA56; LGTN
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for SC322496 CCCCAGCATGGCTGCCCCCACGGCTGAGGGCCTGGCAGCTGCTGCGCCCTCGCTTTCTTG
  ACATTCCCTGGCTTCTGTGCTCTCTTCCCCAGGCCACCCCAGCAGACATGTTTGCCAAGG
  CCTTTCGGGTCAAGTCCAACACGGCCATCAAGGGGTCGGACAGGAGAAAGCTTCGAGCTG
  ATGTGACAACTGCTTTCCCCACCCTTGGAACTGATCAAGTCTCTGAGTTAGTACCTGGAA
  AGGAGGAGCTCAACATTGTGAAGTTGTATGCTCACAAAGGGGATGCAGTGACTGTGTACG
  TGAGTGGTGGTAACCCCATCCTCTTTGAACTGGAGAAAAATCTGTATCCAACAGTGTACA
  CGCTGTGGTCCTATCCTGATCTTCTGCCAACCTTTACAACATGGCCTCTGGTGCTCGAGA
  AACTGGTAGGGGGAGCAGATTTGATGCTGCCTGGACTGGTGATGCCCCCTGCTGGTCTGC
  CTCAGGTACAGAAGGGCGACCTCTGTGCCATTTCTTTGGTGGGGAACAGAGCCCCTGTAG
  CCATTGGAGTTGCAGCCATGTCCACAGCTGAGATGCTCACGTCAGGCCTGAAGGGAAGGG
  GCTTCTCTGTGCTCCACACTTACCAGGACCACTTGTGGCGGTCTGGAAACAAGTCCTCTC
  CACCTTCCATTGCTCCACTGGCCCTGGATTCAGCAGATCTCAGTGAAGAGAAGGGGTCTG
  TCCAGATGGACTCCACCCTGCAGGGAGACATGAGGCACATGACCCTGGAGGGGGAAGAGG
  AGAATGGGGAGGTTCACCAGGCACGTGAAGACAAGTCTCTCTCAGAAGCCCCAGAAGACA
  CCAGCACCAGGGGCCTGAACCAAGACTCCACAGATAGCAAAACGCTTCAAGAACAAATGG
  ATGAGCTGTTACAGCAATGCTTCTTACATGCCTTGAAGTGCCGAGTCAAAAAGGCTGACC
  TCCCTTTACTCACCAGCACTTTCCTTGGCAGCCACATGTTCTCCTGCTGCCCCGAAGGAC
  GACAACTGGACATAAAGAAGTCAAGCTACAAAAAGCTCTCTAAGTTCCTGCAGCAAATGC
  AGCAGGAGCAGATTATACAGGTGAAGGAGCTGAGCAAAGGGGTGGAGAGCATTGTGGCTG
  TGGACTGGAAACACCCGAGGATTACATCTTTCGTCATACCCGAGCCCTCCCCGACCTCCC
  AGACTATCCAGGAGGGTAGCAGGGAACAGCCCTATCACCCTCCAGATATAAAACCCCTCT
  ACTGTGTCCCAGCCAGCATGACCCTGCTCTTCCAGGAGTCTGGCCACAAGAAGGGGAGCT
  TTCTGGAGGGCAGTGAGGTCCGAACGATCGTCATTAACTACGCCAAGAAAAATGACCTGG
  TTGATGCAGACAACAAAAATCTTGTGAGATTGGATCCCATCCTATGTGACTGCATCTTAG
  AGAAAAATGAACAGCATACAGTCATGAAGCTTCCATGGGACAGTCTTCTGACCAGGTGTT
  TGGAAAAATTACAGCCTGCCTATCAAGTGACCCTTCCCGGACAAGAGCCCATTGTGAAGA
  AAGGGAGAATCTGTCCAATTGACATCACCCTAGCACAAAGAGCGTCTAATAAAAAGGTGA
  CCGTGGTCCGGAACTTGGAGGCCTATGGTCTGGACCCATACTCAGTGGCTGCCATCCTTC
  AGCAGCGATGCCAGGCTAGCACCACCGTCAATCCTGCCCCTGGGGCCAAGGACAGCCTTC
  AGGTGCAGATCCAGGGAAACCAGGTCCACCACCTCGGCTGGCTATTGCTTGAAGAGTATC
  AGCTCCCTCGAAAACACATCCAAGGTCTAGAAAAGGCCCTCAAACCTGGCAAGAAGAAGT
  GACAGACTCTTTTGTCTCACGTGGTGGATCCGGTGGAAATCCAAGCTCTGGGCTGGTAAT
  TTTTATGAGCATTTTCAGCTTTTGCAAATACAAAATATAATTCTTTACAAAAATAAATTT
  TTATTCTAATCTAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_006893
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006893.2, NP_008824.2
• RefSeq Size: 2115 bp
• RefSeq ORF: 1755 bp
• Locus ID: 1939
• Cytogenetics: 1q32.1
• Domains: SUI1, PUA
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a translation initiation factor involved in the recruitment and delivery of aminoacyl-tRNAs to the P-site of the eukaryotic ribosome in a GTP-independent manner. This gene was previously referred to as ligatin, but is now known to localize to the cytoplasm and localize and function with translation factors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).