GLO1 (NM_006708) Human Untagged Clone

CAT#: SC115924

GLO1 (untagged)-Human glyoxalase I (GLO1)


  "NM_006708" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol GLO1
Synonyms GLOD1; GLYI; HEL-S-74
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC115924 sequence for NM_006708 edited (data generated by NextGen Sequencing)
ATGGCAGAACCGCAGCCCCCGTCCGGCGGCCTCACGGACGAGGCCGCCCTCAGTTGCTGC
TCCGACGCGGACCCCAGTACCAAGGATTTTCTATTGCAGCAGACCATGCTACGAGTGAAG
GATCCTAAGAAGTCACTGGATTTTTATACTAGAGTTCTTGGAATGACGCTAATCCAAAAA
TGTGATTTTCCCATTATGAAGTTTTCACTCTACTTCTTGGCTTATGAGGATAAAAATGAC
ATCCCTAAAGAAAAAGATGAAAAAATAGCCTGGGCGCTCTCCAGAAAAGCTACACTTGAG
CTGACACACAATTGGGGCACTGAAGATGATGAGACCCAGAGTTACCACAATGGCAATTCA
GACCCTCGAGGATTCGGTCATATTGGAATTGCTGTTCCTGATGTATACAGTGCTTGTAAA
AGGTTTGAAGAACTGGGAGTCAAATTTGTGAAGAAACCTGATGATGGTAAAATGAAAGGC
CTGGCATTTATTCAAGATCCTGATGGCTACTGGATTGAAATTTTGAATCCTAACAAAATG
GCAACCTTAATGTAG

Clone variation with respect to NM_006708.2
>OriGene 5' read for NM_006708 unedited
GTAATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGGGTCGTCTGTGATACTGC
AGCGCAGCCATGGCAGAACCGCAGCCCCCGTCCGGCGGCCTCACGGACGAGGCCGCCCTC
AGTTGCTGCTCCGACGCGGACCCCAGTACCAAGGATTTTCTATTGCAGCAGACCATGCTA
CGAGTGAAGGATCCTAAGAAGTCACTGGATTTTTATACTAGAGTTCTTGGAATGACGCTA
ATCCAAAAATGTGATTTTCCCATTATGAAGTTTTCACTCTACTTCTTGGCTTATGAGGAT
AAAAATGACATCCCTAAAGAAAAAGATGAAAAAATAGCCTGGGCGCTCTCCAGAAAAGCT
ACACTTGAGCTGACACACAATTGGGGCACTGAAGATGATGAGACCCAGAGTTACCACAAT
GGCAATTCAGACCCTCGAGGATTCGGTCATATTGGAATTGCTGTTCCTGATGTATACAGT
GCTTGTAAAAGGTTTGAAGAACTGGGAGTCAAATTTGTGAAGAAACCTGATGATGGTAAA
ATGAAAGGCCTGGCATTTATTCAAGATCCTGATGGCTACTGGATTGAAATTNTGAATCCT
AACAAAATGGCAACCTTAATGTAGTGCTGTGAGAATTCTCCTTTGAGATTTCAGAAGAAA
GGAAACAATGTGATTCAAGATATTTACATACCAGAAGCATCTAGGACTGATGGATCACTG
TCCCCGATCNATTATTCTTCAGTCCATTTCCCCTTCCTATNTCAGCTGTTCCTTTTCACC
TAACTGTTCAGTCATTCTGGTTTTCAAGCAGTGCTTTATCTCATGTCCTGAATATAGNTG
NNGTACTTTATTTTTTAGTAATAATANAACAGTTCCCTTCAGAGCTGCATTTGCCTCTTC
TGCCACTAAATATTNACTCCCTTCAATCTGCT
>OriGene 3' read for NM_006708 unedited
GGTTTTTCAGGGGNNCTNTNATNTATTCCAGTTCTCTCAAGCTATTGGCTATCTTGTCTT
AAAGCACTCACATAAATCCATTTCACCCAAAAAGGAAACATAAAGTGCTTCTAGCAGTAC
AAGCACGGTTGGCATGGCCTTTCCAAAGGTCTTCCACTAGAGTCTAGAGAAATCTAAATA
TAGTCATCCACAAACTGGATGTTTTTATTTTCTGAGCCATTAGAGATTTTCAAAATCACT
TTGATTTTTAAAAACTCATCAAATGTGAATCATGGCGGGGAAGACCACTGAGCTGATTTC
TGATAACTAAGTTATCACTGAACATAATTTATCATATATGGCTACTGGCATCATGAAGAC
CTTGGGATAGGGAAGACTCTTTATGAGAAATATAAACATCACTTGTGTAGGAATCACCAG
GTGTCCCTAGAGCAGTTTTGTACTAAAGACTTCTAGTGTTTACTNCCTCACCACGGGTAC
CTTCCCAACCNNNNNNNNNNNCNCCCCNNCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
NCCCCCCCCCCCCCCCCCCCCNCCCCCCCCCCCCCCCCCCCCCCCCCCCCCNCCCCCCCC
CCCNCCCCCNNNCCCCCCCCCCCCNCCNCCCCCCCCCCCCCCCCCCCCCCCCCCCCNCCC
CCACCCCCNCCNACCCCCCCCCCCCCCCCCNCCCCCCNCCCCCCCCCCCCCCCCNCNCCC
CCCCCCCCNCCCCCCCCCCCCNCCCCCCNCCAACACACCCCCCCCCCCCCCACCCCCCCC
CCCACCCCCCCCCCCACCCACCCCCCACACAACCCCCACCCCCACCCACCCCACCCCCCA
CACCCCCCCCACCCCCCCACACCACCCNCCCCCCCACNCCACCACCCCCCCACCACACC
Restriction Sites NotI-NotI     
ACCN NM_006708
Insert Size 2100 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006708.1, NP_006699.1
RefSeq Size 1993 bp
RefSeq ORF 555 bp
Locus ID 2739
Cytogenetics 6p21.2
Domains Glyoxalase
Protein Pathways Pyruvate metabolism
Gene Summary 'The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq, Jul 2008]'

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