Hsc70 (HSPA8) (NM_006597) Human Untagged Clone

CAT#: SC115973

HSPA8 (untagged)-Human heat shock 70kDa protein 8 (HSPA8), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HSPA8
• Synonyms: HEL-33; HEL-S-72p; HSC54; HSC70; HSC71; HSP71; HSP73; HSPA10; LAP-1; LAP1; NIP71
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115973 sequence for NM_006597 edited (data generated by NextGen Sequencing)
  ATGTCCAAGGGACCTGCAGTTGGTATTGATCTTGGCACCACCTACTCTTGTGTGGGTGTT
  TTCCAGCACGGAAAAGTCGAGATAATTGCCAATGATCAGGGAAACCGAACCACTCCAAGC
  TATGTCGCCTTTACGGACACTGAACGGTTGATCGGTGATGCCGCAAAGAATCAAGTTGCA
  ATGAACCCCACCAACACAGTTTTTGATGCCAAACGTCTGATTGGACGCAGATTTGATGAT
  GCTGTTGTCCAGTCTGATATGAAACATTGGCCCTTTATGGTGGTGAATGATGCTGGCAGG
  CCCAAGGTCCAAGTAGAATACAAGGGAGAGACCAAAAGCTTCTATCCAGAGGAGGTGTCT
  TCTATGGTTCTGACAAAGATGAAGGAAATTGCAGAAGCCTACCTTGGGAAGACTGTTACC
  AATGCTGTGGTCACAGTGCCAGCTTACTTTAATGACTCTCAGCGTCAGGCTACCAAAGAT
  GCTGGAACTATTGCTGGTCTCAATGTACTTAGAATTATTAATGAGCCAACTGCTGCTGCT
  ATTGCTTACGGCTTAGACAAAAAGGTTGGAGCAGAAAGAAACGTGCTCATCTTTGACCTG
  GGAGGTGGCACTTTTGATGTGTCAATCCTCACTATTGAGGATGGAATCTTTGAGGTCAAG
  TCTACAGCTGGAGACACCCACTTGGGTGGAGAAGATTTTGACAACCGAATGGTCAACCAT
  TTTATTGCTGAGTTTAAGCGCAAGCATAAGAAGGACATCAGTGAGAACAAGAGAGCTGTA
  AGACGCCTCCGTACTGCTTGTGAACGTGCTAAGCGTACCCTCTCTTCCAGCACCCAGGCC
  AGTATTGAGATCGATTCTCTCTATGAAGGAATCGACTTCTATACCTCCATTACCCGTGCC
  CGATTTGAAGAACTGAATGCTGACCTGTTCCGTGGCACCCTGGACCCAGTAGAGAAAGCC
  CTTCGAGATGCCAAACTAGACAAGTCACAGATTCATGATATTGTCCTGGTTGGTGGTTCT
  ACTCGTATCCCCAAGATTCAGAAGCTTCTCCAAGACTTCTTCAATGGAAAAGAACTGAAT
  AAGAGCATCAACCCTGATGAAGCTGTTGCTTATGGTGCAGCTGTCCAGGCAGCCATCTTG
  TCTGGAGACAAGTCTGAGAATGTTCAAGATTTGCTGCTCTTGGATGTCACTCCTCTTTCC
  CTTGGTATTGAAACTGCTGGTGGAGTCATGACTGTCCTCATCAAGCGTAATACCACCATT
  CCTACCAAGCAGACACAGACCTTCACTACCTATTCTGACAACCAGCCTGGTGTGCTTATT
  CAGGTTTATGAAGGCGAGCGTGCCATGACAAAGGATAACAACCTGCTTGGCAAGTTTGAA
  CTCACAGGCATACCTCCTGCACCCCGAGGTGTTCCTCAGATTGAAGTCACTTTTGACATT
  GATGCCAATGGTATACTCAATGTCTCTGCTGTGGACAAGAGTACGGGAAAAGAGAACAAG
  ATTACTATCACTAATGACAAGGGCCGTTTGAGCAAGGAAGACATTGAACGTATGGTCCAG
  GAAGCTGAGAAGTACAAAGCTGAAGATGAGAAGCAGAGGGACAAGGTGTCATCCAAGAAT
  TCACTTGAGTCCTATGCCTTCAACATGAAAGCAACTGTTGAAGATGAGAAACTTCAAGGC
  AAGATTAACGATGAGGACAAACAGAAGATTCTGGACAAGTGTAATGAAATTATCAACTGG
  CTTGATAAGAATCAGACTGCCGAGAAGGAAGAATTTGAACATCAACAGAAAGAGCTGGAG
  AAAGTTTGCAACCCCATCATCACCAAGCTGTACCAGAGTGCAGGAGGCATGCCAGGAGGA
  ATGCCTGGGGGATTTCCTGGTGGTGGAGCTCCTCCCTCTGGTGGTGCTTCCTCAGGGCCC
  ACCATTGAAGAGGTTGATTAA
  
  Clone variation with respect to NM_006597.4
  1761 t=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006597 unedited
  GGTTACATTTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGCTCGCCTGCA
  GCTCTTGGGTTTTTTGTGGCTTCCTTCGTTATTGGAGCCAGGCCTACATCCCAGCAACCA
  TGTCCAAGGGACCTGCAGTTGGTATTGATCTTGGCACCACCTACTCTTGTGTGGGTGTTT
  TCCAGCACGGTAAAAGTCGAGATAATTGCCAATGATCAGGGAAACCGAACCACTCCAAGC
  TATGTCGCCTTTACGGACACTGAACGGTTGATCGGTGATGCCGCAAAGAATCAAGTTGCA
  ATGAACCCCACCAACACAGTTTTTGATGCCAAACGTCTGATTGGACGCAGATTTGATGAT
  GCTGTTGTCCAGTCTGATATGAAACATTGGCCCTTTATGGTGGTGAATGATGCTGGCAGG
  CCCAAGGTCCAAGTAGAATACAAGGGAGAGACCAAAAGCTTCTATCCAGAGGAGGTGTCT
  TCTATGGTTCTGACAAAGATGAAGGAAATTGCAGAAGCCTACCTTGGGAAGACTGTTACC
  AATGCTGTGGTCACAGTGCCAGCTTACTTTAATGACTCTCAGCGTCAGGCTACCAAAGAT
  GCTGGAACTATTGCTGGTCTCAATGTACTTAGAATTATTAATGAGCCAACTGCTGCTGCT
  ATTGCTTACGGCTTAGACAAAAAGGTTGGAGCAGAAAGAAACGTGCTCATCTTTGACCTG
  GGAGGTGGCACTTTTGATGTGTCAATCCTCACTATTNGAGATGGAATCTTTGAGGTCAAG
  TCTACAGCTGGAGACACCCACTTGGGTGGAGAAGATTNTGACAACCGAATGGGTCACCAT
  TTTATTGCTGAGTTTAAGCGCAAGCTTAGAAGGACATCAGTGAGAACAAGAGAGCTGTNA
  GAACGCTCCGTACTGCCTGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006597 unedited
  ATGGCAACTTCCAGGGCCAGNATAGCACTGGGGAGGGGTCACAGGNATGCCACCCGGGAT
  CTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTT
  TTTTGCCAATTTTAAATAGTTTTATTTAAGACATTGCATTTTCCACTTACAATACAGTGT
  TTATAAAGTGCAATGTTATTTCCTTCCCCTGTGCATATGTTCCATATTCAAGTATTGAGA
  ATGCCCAGTAACTTACTATAGCAGCTTAACTTTTTAAAACTGCCACAGAATTTGCTACGA
  ATTTAGGTCCTTCAAATGTTTTAAATGTGTGGAACAATGCTACATCTACACTTGGTTGGC
  TTAATCAACCTCTTCAATGGTGGGCCCTGAGGAAGCACCACCAGAGGGAGGAGCTCCACC
  ACCAGGAAATCCCCCAGGCATTCCTCCTGGCATGCCTCCTGCACTCTGGTACAGCTTGGT
  GATGATGGGGTTGCAAACTTTCTCCAGCTCTTTCTGTTGATGTTCAAATTCTTCCTTCTC
  GGCAGTCTGATTCTTATCAAGCCAGTTGATAATTTCATTACACTTGTCCAGAATCTTCTG
  TTTGTCCTCATCGTTAATCTTGCCTTGAAGTTTCTCATCTTCAACAGTTGCTTTCATGTT
  GAAGGCATAGGACTCAAGTGAATTCTTGGATGACANCCTTGTCCCTCTGCTTCTCATCTT
  CAGCTTTGTACTTCTCAGCTTCCTGGACCATACGTTCAATGGTCTTCTTGCTCAAACGNC
  CCTTGNATTAGNGATAGTAATCTTGGTCTCTTTTCCCCGTACTCTTNGCCACAGCAGAGA
  CATTGAGGATACCATTGGCATCATGGCAAAAGTGACTTCATCCTGAGACACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006597
• Insert Size: 2190 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006597.3, NP_006588.1
• RefSeq Size: 2276 bp
• RefSeq ORF: 1941 bp
• Locus ID: 3312
• Cytogenetics: 11q24.1
• Domains: HSP70
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Antigen processing and presentation, Endocytosis, MAPK signaling pathway, Spliceosome
• Gene Summary: 'This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]'
  Transcript Variant: This variant (1) represents the predominant transcript, and encodes the longer isoform (1).