CD146 (MCAM) (NM_006500) Human Untagged Clone

CAT#: SC116067

MCAM (untagged)-Human melanoma cell adhesion molecule (MCAM)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MCAM
• Synonyms: CD146; HEMCAM; MelCAM; METCAM; MUC18
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116067 sequence for NM_006500 edited (data generated by NextGen Sequencing)
  ATGGGGCTTCCCAGGCTGGTCTGCGCCTTCTTGCTCGCCGCCTGCTGCTGCTGTCCTCGC
  GTCGCGGGTGTGCCCGGAGAGGCTGAGCAGCCTGCGCCTGAGCTGGTGGAGGTGGAAGTG
  GGCAGCACAGCCCTTCTGAAGTGCGGCCTCTCCCAGTCCCAAGGCAACCTCAGCCATGTC
  GACTGGTTTTCTGTCCACAAGGAGAAGCGGACGCTCATCTTCCGTGTGCGCCAGGGCCAG
  GGCCAGAGCGAACCTGGGGAGTACGAGCAGCGGCTCAGCCTCCAGGACAGAGGGGCTACT
  CTGGCCCTGACTCAAGTCACCCCCCAAGACGAGCGCATCTTCTTGTGCCAGGGCAAGCGC
  CCTCGGTCCCAGGAGTACCGCATCCAGCTCCGCGTCTACAAAGCTCCGGAGGAGCCAAAC
  ATCCAGGTCAACCCCCTGGGCATCCCTGTGAACAGTAAGGAGCCTGAGGAGGTCGCTACC
  TGTGTAGGGAGGAACGGGTACCCCATTCCTCAAGTCATCTGGTACAAGAATGGCCGGCCT
  CTGAAGGAGGAGAAGAACCGGGTCCACATTCAGTCGTCCCAGACTGTGGAGTCGAGTGGT
  TTGTACACCTTGCAGAGTATTCTGAAGGCACAGCTGGTTAAAGAAGACAAAGATGCCCAG
  TTTTACTGTGAGCTCAACTACCGGCTGCCCAGTGGGAACCACATGAAGGAGTCCAGGGAA
  GTCACCGTCCCTGTTTTCTACCCGACAGAAAAAGTGTGGCTGGAAGTGGAGCCCGTGGGA
  ATGCTGAAGGAAGGGGACCGCGTGGAAATCAGGTGTTTGGCTGATGGCAACCCTCCACCA
  CACTTCAGCATCAGCAAGCAGAACCCCAGCACCAGGGAGGCAGAGGAAGAGACAACCAAC
  GACAACGGGGTCCTGGTGCTGGAGCCTGCCCGGAAGGAACACAGTGGGCGCTATGAATGT
  CAGGGCCTGGACTTGGACACCATGATATCGCTGCTGAGTGAACCACAGGAACTACTGGTG
  AACTATGTGTCTGACGTCCGAGTGAGTCCCGCAGCCCCTGAGAGACAGGAAGGCAGCAGC
  CTCACCCTGACCTGTGAGGCAGAGAGTAGCCAGGACCTCGAGTTCCAGTGGCTGAGAGAA
  GAGACAGGCCAGGTGCTGGAAAGGGGGCCTGTGCTTCAGTTGCATGACCTGAAACGGGAG
  GCAGGAGGCGGCTATCGCTGCGTGGCGTCTGTGCCCAGCATACCCGGCCTGAACCGCACA
  CAGCTGGTCAACGTGGCCATTTTTGGCCCCCCTTGGATGGCATTCAAGGAGAGGAAGGTG
  TGGGTGAAAGAGAATATGGTGTTGAATCTGTCTTGTGAAGCGTCAGGGCACCCCCGGCCC
  ACCATCTCCTGGAACGTCAACGGCACGGCAAGTGAACAAGACCAAGATCCACAGCGAGTC
  CTGAGCACCCTGAATGTCCTCGTGACCCCGGAGCTGTTGGAGACAGGTGTTGAATGCACG
  GCCTCCAACGACCTGGGCAAAAACACCAGCATCCTCTTCCTGGAGCTGGTCAATTTAACC
  ACCCTCACACCAGACTCCAACACAACCACTGGCCTCAGCACTTCCACTGCCAGTCCTCAT
  ACCAGAGCCAACAGCACCTCCACAGAGAGAAAGCTGCCGGAGCCGGAGAGCCGGGGCGTG
  GTCATCGTGGCTGTGATTGTGTGCATCCTGGTCCTGGCGGTGCTGGGCGCTGTCCTCTAT
  TTCCTCTATAAGAAGGGCAAGCTGCCGTGCAGGCGCTCAGGGAAGCAGGAGATCACGCTG
  CCCCCGTCTCGTAAGAGCGAACTTGTAGTTGAAGTTAAGTCAGATAAGCTCCCAGAAGAG
  ATGGGCCTCCTGCAGGGCAGCAGCGGTGACAAGAGGGCTCCGGGAGACCAGGGAGAGAAA
  TACATCGATCTGAGGCATTAG
  
  Clone variation with respect to NM_006500.2
  1800 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006500 unedited
  TAACGTCAAATTTTGTATACGACTCCTATAGGCGGCACGCGAATTCGCACGAGAGGGGCT
  TCCCAGGCTGGTCTGCGCCTTCTTGCTCGCCGCCTGCTGCTGCTGTCCTCGCGTCGCGGG
  TGTGCCCGGAGAGGCTGAGCAGCCTGCGCCTGAGCTGGTGGAGGTGGAAGTGGGCAGCAC
  AGCCCTTCTGAAGTGCGGCCTCTCCCAGTCCCAAGGCAACCTCAGCCATGTCGACTGGTT
  TTCTGTCCACAAGGAGAAGCGGACGCTCATCTTCCGTGTGCGCCAGGGCCAGGGCCAGAG
  CGAACCTGGGGAGTACGAGCAGCGGCTCAGCCTCCAGGACAGAGGGGCTACTCTGGCCCT
  GACTCAAGTCACCCCCCAAGACGAGCGCATCTTCTTGTGCCAGGGCAAGCGCCCTCGGTC
  CCAGGAGTACCGCATCCAGCTCCGCGTCTACAAAGCTCCGGAGGAGCCAAACATCCAGGT
  CAACCCCCTGGGCATCCCTGTGAACAGTAAGGAGCCTGAGGAGGTCGCTACCTGTGTAGG
  GAGGAACGGGTACCCCATTCCTCAAGTCATCTGGTACAAGAATGGCCGGCCTCTGAAGGA
  GGAGAAGAACCGGGTCCACATTCAGTCGTCCCAGACTGTGGAGTCGAGTGGTTTGTACAC
  CTTGCAGAGTATTCTGAAGGCACAGCTGGTTAAAGAAGACAAAGATGCCCAGTTTTACTG
  TGAGCTCAACTACCGGCTGCCCAGTGGGAACCACATGAAGGAGTCCAGGGAAGTCACCGT
  CCCTGTTTTCTNACCCGACAGATAAGTGTGGCTGGAAGTGGAGCCCGTGGGAATGCTGAA
  NGAGGGGACCGCGTGGAATCATGTGTTTGGGCTGATGGCACCCTNCACCACACTTCAGCA
  TCAGCAAGCATGATCCCAGCACC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006500 unedited
  CTATGTAACGCGGCACGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTGATTTCTGGGG
  CAATTAAGCTTTATTTTTCATATATATATATATTCTTCATATATATATATACATACATAT
  ATAAAGGAAACAATTTGCAAATTTACACACCTGACAAAACCATATATACACACATATGTA
  TGCATACACACAGACAGACACACACACCCGAAGCTCTAGCCAGGCCCGTTTTCCATCCCT
  AAGTACCATTCTCTCATTTGGGCCCTTCTAAGGTTGGGGCCCTGAGCTTGGTTTGTAGAA
  GTTTGGTGCTAATATAACCATAGCTTTAATCCCCATGAAGGACAGTGTAGACCTCATCTT
  TGTCTGCTCCCCGCTGCCTTTCAGTTTTACGTGATCCATCAAGAGGGCTATGGGAGCCAA
  GTGAACACGGGGGATTGAGGCTAATTCACCTGAACTCGAAAACAGCGCCCAGCTTCCTCA
  CTGCAAGCACGTGTCTTTTCTTTTTTTTTTCTCGAGACGGAGTCTCGCTGTGTTGCCCAA
  GCTGGAGTGCAGTGGCACGGTCTCGGCTCACTGCAAGCTCCACCTCCTGGATTCATACCA
  TTCTCCTGCTTCAAGCCTTCGAGTAGCTGGGACTATATGTGCCAACCACTACGCCTAGCT
  AACTCCTTCTAGATTTTTAATACACACAGGCTTTCACCGGGTTAACCACGATGGACTTGT
  CCTGAATTTGTGATCCGCCCGCCTCGGCCCACAAAAGGCCGGGATTACAGGTGTGAGACA
  CCACACCTGGCCCCGGAACGTATTTTTAAAGAAGACACCAATTCTTGGTTTTGCACAAGA
  AAATAGTCAAGGATATTTGTACAGCACAATGAGGGTGGGGCCCACCCTGCAACTGCTATG
  GATGCTTCGGGGGGTTCTGCAGCGGNGGTACAAACCACTAAACTTATCGGCCCCGCACCG
  GGAACCTCTTAACGTGGCCCGGGAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006500
• Insert Size: 3460 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006500.2, NP_006491.2
• RefSeq Size: 3332 bp
• RefSeq ORF: 1941 bp
• Locus ID: 4162
• Cytogenetics: 11q23.3
• Domains: ig, IGc2, IG
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Gene Summary: ''