Ribonuclease H2, subunit A (RNASEH2A) (NM_006397) Human Untagged Clone

CAT#: SC116088

RNASEH2A (untagged)-Human ribonuclease H2, subunit A (RNASEH2A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RNASEH2A
• Synonyms: AGS4; JUNB; RNASEHI; RNHIA; RNHL; THSD8
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116088 sequence for NM_006397 edited (data generated by NextGen Sequencing)
  ATGGATCTCAGCGAGCTGGAGAGAGACAATACAGGCCGCTGTCGCCTGAGTTCGCCTGTG
  CCCGCGGTGTGCCGCAAGGAGCCTTGCGTCCTGGGCGTCGATGAGGCGGGCAGGGGCCCC
  GTGCTGGGCCCCATGGTCTACGCCATCTGTTATTGTCCCCTGCCTCGCCTGGCAGATCTG
  GAGGCGCTGAAAGTGGCAGACTCAAAGACCCTATTGGAGAGCGAGCGGGAAAGGCTGTTT
  GCGAAAATGGAGGACACGGACTTTGTCGGCTGGGCGCTGGATGTGCTGTCTCCAAACCTC
  ATCTCTACCAGCATGCTTGGGCGGGTCAAATACAACCTGAACTCCCTGTCACATGATACA
  GCCACTGGGCTTATACAGTATGCATTGGACCAGGGCGTGAACGTCACCCAGGTATTCGTG
  GACACCGTAGGGATGCCAGAGACATACCAGGCGCGGCTGCAGCAAAGTTTTCCCGGGATT
  GAGGTGACGGTCAAGGCCAAAGCAGATGCCCTCTACCCGGTGGTTAGTGCTGCCAGCATC
  TGTGCCAAGGTGGCCCGGGACCAGGCCGTGAAGAAATGGCAGTTCGTGGAGAAACTGCAG
  GACTTGGATACTGATTATGGCTCAGGCTACCCCAATGATCCCAAGACAAAAGCGTGGTTG
  AAGGAGCACGTGGAGCCTGTGTTCGGCTTCCCCCAGTTTGTCCGGTTCAGCTGGCGCACG
  GCCCAGACCATCCTGGAGAAAGAGGCGGAAGATGTTATATGGGAGGACTCAGCATCCGAG
  AATCAGGAGGGACTCAGGAAGATCACATCCTACTTCCTCAATGAAGGGTCCCAAGCCCGT
  CCCCGTTCTTCCCACCGATATTTCCTGGAACGCGGCCTGGAGTCAGCAACCAGCCTCTAG
  
  Clone variation with respect to NM_006397.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006397 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGGCTGAGGCGGCATGGA
  TCTCAGCGAGCTGGAGAGAGACAATACAGGCCGCTGTCGCCTGAGTTCGCCTGTGCCCGC
  GGTGTGCCGCAAGGAGCCTTGCGTCCTGGGCGTCGATGAGGCGGGCAGGGGCCCCGTGCT
  GGGCCCCATGGTCTACGCCATCTGTTATTGTCCCCTGCCTCGCCTGGCAGATCTGGAGGC
  GCTGAAAGTGGCAGACTCAAAGACCCTATTGGAGAGCGAGCGGGAAAGGCTGTTTGCGAA
  AATGGAGGACACGGACTTTGTCGGCTGGGCGCTGGATGTGCTGTCTCCAAACCTCATCTC
  TACCAGCATGCTTGGGCGGGTCAAATACAACCTGAACTCCCTGTCACATGATACAGCCAC
  TGGGCTTATACAGTATGCATTGGACCAGGGCGTGAACGTCACCCAGGTATTCGTGGACAC
  CGTAGGGATGCCAGAGACATACCAGGCGCGGCTGCAGCAAAGTTTTCCCGGGATTGAGGT
  GACGGTCAAGGCCAAAGCAGATGCCCTCTACCCGGTGGTTAGTGCTGCCAGCATCTGTGC
  CAAGGTGGCCCCGGACCANGCCGTGAAGAAATGGCAGTTCGTGGAGAAACTGCAAGACTT
  GGATACTGATTATGGCTCAGGCTACCCCCATGATCCCAAGACAAAGCGTGGTTGAAGAGC
  ACGTGAGCCCTGTGTTCGGCTTCCCCAGTTTTGCCCGGTCAGTTGGCGCCCGGCCCAAAC
  ATCCTGGAGAAGAAGCGGGAAATGTATATTGGAGACCCACCTCCCAGAATCCGAGGCCCT
  CNGAAAACCCTTCTATTTCTTATGAAGGGGGCCAGCCCGCCCCGTTTTTCCCCGATTTTC
  CTGGAAGCGGCTGGAATACACCCATCTTACCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006397 unedited
  ACCGCGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAATCAACCAACACCCA
  TTCTATTTAAGGTTCCAAAAGGAAGTAGCTGGACCCGGCTGCAGAGCACACTCCCACCTT
  GCTTCTGTCCCAAAAGTACATCCCCTACGTGTGGTTCTCCTTAAACAATTTTAATGTCTG
  GGTTGGGGAAGCAGGTAGAGCGCGTAGAGGCAGCTGCTAAAGGCTGGTTGCTGACTCCAG
  GCCGCGTTCCAGGAAATATCGGTGGGAAGAACGGGGACGGGCTTGGGACCCTTCATTGAG
  GAAGTAGGATGTGATCTTCCTGAGTCCCTCCTGATTCTCGGATGCTGAGTCCTCCCATAT
  AACATCTTCCGCCTCTTTCTCCAGGATGGTCTGGGCCGTGCGCCAGCTGAACCGGACAAA
  CTGGGGGAAGCCGAACACAGGCTCCACGTGCTCCTTCAACCACGCTTTTGTCTTGGGATC
  ATTGGGGTAGCCTGAGCCATAATCAGTATCCAAGTCCTGCAGTTTCTCCACGAACTGCCA
  TTTCTTCACGGCCTGGTCCCGGGCCACCTTGGCACAGATGCTGGCAGCACTAACCACCGG
  GTAGAGGGCATCTGCTTTGGCCTTGACCGTCACCTCAATCCCGGGAAAACTTTGCTGCAG
  CCGCGCCTGGTATGTCTCTGGCATCCCTACAGTGTCCACGATTACCTGGGTGACGTTCAC
  GCCCTGGTCCCATGCATACTGTATAGCCCAGTGGCTGATCATGTGACGGAAGTTCAGTTG
  TATTGACCCCCCAAACTGCTGTAAAGAGAGTTTGGAACGCCAATCACGCCCACCGACAAT
  TCGTGCCTCCTTTTCCAAAAGCCTTCCCCTCCCTCCAAAGGCCTTGAGTCGCCTTTATGC
  CTCAATTGCCGCGAGAGGGACTTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006397
• ORF Size: 900 bp
• Insert Size: 1140
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_006397.2, NP_006388.2
• RefSeq Size: 1148
• RefSeq ORF: 900
• Locus ID: 10535
• Domains: RNase_HII
• Protein Pathways: DNA replication
• Gene Summary: The protein encoded by this gene is a component of the heterotrimeric type II ribonuclease H enzyme (RNAseH2). RNAseH2 is the major source of ribonuclease H activity in mammalian cells and endonucleolytically cleaves ribonucleotides. It is predicted to remove Okazaki fragment RNA primers during lagging strand DNA synthesis and to excise single ribonucleotides from DNA-DNA duplexes. Mutations in this gene cause Aicardi-Goutieres Syndrome (AGS), a an autosomal recessive neurological disorder characterized by progressive microcephaly and psychomotor retardation, intracranial calcifications, elevated levels of interferon-alpha and white blood cells in the cerebrospinal fluid. [provided by RefSeq, Aug 2009]