Ribonuclease H2, subunit A (RNASEH2A) (NM_006397) Human Untagged Clone

CAT#: SC320899

RNASEH2A (untagged)-Human ribonuclease H2, subunit A (RNASEH2A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RNASEH2A
• Synonyms: AGS4; JUNB; RNASEHI; RNHIA; RNHL; THSD8
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006397.2 CCCGCTCCTGCAGTATTAGTTCTTGCAGCTGGTGGTGGCGGCTGAGGCGGCATGGATCTC
  AGCGAGCTGGAGAGAGACAATACAGGCCGCTGTCGCCTGAGTTCGCCTGTGCCCGCGGTG
  TGCCGCAAGGAGCCTTGCGTCCTGGGCGTCGATGAGGCGGGCAGGGGCCCCGTGCTGGGC
  CCCATGGTCTACGCCATCTGTTATTGTCCCCTGCCTCGCCTGGCAGATCTGGAGGCGCTG
  AAAGTGGCAGACTCAAAGACCCTATTGGAGAGCGAGCGGGAAAGGCTGTTTGCGAAAATG
  GAGGACACGGACTTTGTCGGCTGGGCGCTGGATGTGCTGTCTCCAAACCTCATCTCTACC
  AGCATGCTTGGGCGGGTCAAATACAACCTGAACTCCCTGTCACATGATACAGCCACTGGG
  CTTATACAGTATGCATTGGACCAGGGCGTGAACGTCACCCAGGTATTCGTGGACACCGTA
  GGGATGCCAGAGACATACCAGGCGCGGCTGCAGCAAAGTTTTCCCGGGATTGAGGTGACG
  GTCAAGGCCAAAGCAGATGCCCTCTACCCGGTGGTTAGTGCTGCCAGCATCTGTGCCAAG
  GTGGCCCGGGACCAGGCCGTGAAGAAATGGCAGTTCGTGGAGAAACTGCAGGACTTGGAT
  ACTGATTATGGCTCAGGCTACCCCAATGATCCCAAGACAAAAGCGTGGTTGAAGGAGCAC
  GTGGAGCCTGTGTTCGGCTTCCCCCAGTTTGTCCGGTTCAGCTGGCGCACGGCCCAGACC
  ATCCTGGAGAAAGAGGCGGAAGATGTTATATGGGAGGACTCAGCATCCGAGAATCAGGAG
  GGACTCAGGAAGATCACATCCTACTTCCTCAATGAAGGGTCCCAAGCCCGTCCCCGTTCT
  TCCCACCGATATTTCCTGGAACGCGGCCTGGAGTCAGCAACCAGCCTCTAGCAGCTGCCT
  CTACGCGCTCTACCTGCTTCCCCAACCCAGACATTAAAATTGTTTAAGGAGACCCCCAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006397.2 unedited
  GCCCATTGTATACGACTCCTATAGGGCGGCCGGGAATTCGGCACGCAGGCCCGCTCCTGC
  AGTATTAGTTCTTGCAGCTGGTGGTGGCGGCTGAGGCGGCATGGATCTCAGCGAGCTGGA
  GAGAGACAATACAGGCCGCTGTCGCCTGAGTTCGCCTGTGCCCGCGGTGTGCCGCAAGGA
  GCCTTGCGTCCTGGGCGTCGATGAGGCGGGCAGGGGCCCCGTGCTGGGCCCCATGGTCTA
  CGCCATCTGTTATTGTCCCCTGCCTCGCCTGGCAGATCTGGAGGCGCTGAAAGTGGCAGA
  CTCAAAGACCCTATTGGAGAGCGAGCGGGAAAGGCTGTTTGCGAAAATGGAGGACACGGA
  CTTTGTCGGCTGGGCGCTGGATGTGCTGTCTCCAAACCTCATCTCTACCAGCATGCTTGG
  GCGGGTCAAATACAACCTGAACTCCCTGTCACATGATACAGCCACTGGGCTTATACAGTA
  TGCATTGGACCAGGGCGTGAACGTCACCCAGGTATTCGTGGACACCGTAGGGATGCCAGA
  GACATACCAGGCGCGGCTGCAGCAAAGTTTTCCCGGGATTGAGGTGACGGTCAAGGCCAA
  AGCAGATGCCCTCTACCCGGTGGTTAGTGCTGCCAGCATCTGTGCCAAGGTGGCCCGGGA
  CCAGGCCCGTGAAGAAATGGCAGTTCGTGGAGAAACTGCAAGGACTTGGATACTGATTAT
  GGCTCAGCTACCCCAATGATCCCAAGACAAAAGCGTGTTGAAGGAGCACGTGGAGCCTGG
  TGTTCGGCTTCCCAGTTTTGTCCCGTTCAGCTGGCGCCACGGCCAGACCATTCCTGAAAA
  GAGCGAAGAATGTTATTATGGGAGACTCAGCATCGGATATCAGAGACTCAGAAGATTCAA
  ATCTACTCTCATGAGGTCCAAGCCGTCCCGGTTCTTCCCACCGGAA
• Restriction Sites: Please inquire
• ACCN: NM_006397
• ORF Size: 900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_006397.2, NP_006388.2
• RefSeq Size: 1148
• RefSeq ORF: 900
• Locus ID: 10535
• Domains: RNase_HII
• Protein Pathways: DNA replication
• Gene Summary: The protein encoded by this gene is a component of the heterotrimeric type II ribonuclease H enzyme (RNAseH2). RNAseH2 is the major source of ribonuclease H activity in mammalian cells and endonucleolytically cleaves ribonucleotides. It is predicted to remove Okazaki fragment RNA primers during lagging strand DNA synthesis and to excise single ribonucleotides from DNA-DNA duplexes. Mutations in this gene cause Aicardi-Goutieres Syndrome (AGS), a an autosomal recessive neurological disorder characterized by progressive microcephaly and psychomotor retardation, intracranial calcifications, elevated levels of interferon-alpha and white blood cells in the cerebrospinal fluid. [provided by RefSeq, Aug 2009]