HEF1 (NEDD9) (NM_006403) Human Untagged Clone

CAT#: SC116092

NEDD9 (untagged)-Human neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NEDD9
• Synonyms: CAS-L; CAS2; CASL; CASS2; HEF1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116092 sequence for NM_006403 edited (data generated by NextGen Sequencing)
  ATGAAGTATAAGAATCTTATGGCAAGGGCCTTATATGACAATGTCCCAGAGTGTGCCGAG
  GAACTGGCCTTTCGCAAGGGAGACATCCTGACCGTCATAGAGCAGAACACAGGGGGACTG
  GAAGGATGGTGGCTGTGCTCGTTACACGGTCGGCAAGGCATTGTCCCAGGCAACCGGGTG
  AAGCTTCTGATTGGTCCCATGCAGGAGACTGCCTCCAGTCACGAGCAGCCTGCCTCTGGA
  CTGATGCAGCAGACCTTTGGCCAACAGAAGCTCTATCAAGTGCCAAACCCACAGGCTGCT
  CCCCGAGACACCATCTACCAAGTGCCACCTTCCTACCAAAATCAGGGAATTTACCAAGTC
  CCCACTGGCCACGGCACCCAAGAACAAGAGGTATATCAGGTGCCACCATCAGTGCAGAGA
  AGCATTGGGGGAACCAGTGGGCCCCACGTGGGTAAAAAGGTGATAACCCCCGTGAGGACA
  GGCCATGGCTACGTATACGAGTACCCATCCAGATACCAAAAGGATGTCTATGATATCCCT
  CCTTCTCATACCACTCAAGGGGTATACGACATCCCTCCCTCATCAGCAAAAGGCCCTGTG
  TTTTCAGTTCCAGTGGGAGAGATAAAACCTCAAGGGGTGTATGACATCCCGCCTACAAAA
  GGGGTATATGCCATTCCGCCCTCTGCTTGCCGGGATGAAGCAGGGCTTAGGGAAAAAGAC
  TATGACTTCCCCCCTCCCATGAGACAAGCTGGAAGGCCGGACCTCAGACCGGAGGGGGTT
  TATGACATTCCTCCAACCTGCACCAAGCCAGCAGGGAAGGACCTTCATGTAAAATACAAC
  TGTGACATTCCAGGAGCTGCAGAACCGGTGGCTCGAAGGCACCAGAGCCTGTCCCCGAAT
  CACCCACCCCCGCAACTCGGACAGTCAGTGGGCTCTCAGAACGACGCATATGATGTCCCC
  CGAGGCGTTCAGTTTCTTGAGCCACCAGCAGAAACCAGTGAGAAAGCAAACCCCCAGGAA
  AGGGATGGTGTTTATGATGTCCCTCTGCATAACCCGCCAGATGCTAAAGGCTCTCGGGAC
  TTGGTGGATGGGATCAACCGATTGTCTTTCTCCAGTACAGGCAGCACCCGGAGTAACATG
  TCCACGTCTTCCACCTCCTCCAAGGAGTCCTCACTGTCAGCCTCCCCAGCTCAGGACAAA
  AGGCTCTTCCTGGATCCAGACACAGCTATTGAGAGACTTCAGCGGCTCCAGCAGGCCCTT
  GAGATGGGTGTCTCCAGCCTAATGGCACTGGTCACTACCGACTGGCGGTGTTACGGATAT
  ATGGAAAGACACATCAATGAAATACGCACAGCAGTGGACAAGGTGGAGCTGTTCCTGAAG
  GAGTACCTCCACTTTGTCAAGGGAGCTGTTGCAAATGCTGCCTGCCTCCCGGAACTCATC
  CTCCACAACAAGATGAAGCGGGAGCTGCAACGAGTCGAAGACTCCCACCAGATCCTGAGT
  CAAACCAGCCATGACTTAAATGAGTGCAGCTGGTCCCTGAATATCTTGGCCATCAACAAG
  CCCCAGAACAAGTGTGACGATCTGGACCGGTTTGTGATGGTGGCAAAGACGGTGCCCGAT
  GACGCCAAGCAGCTCACCACAACCATCAACACCAACGCAGAGGCCCTCTTCAGACCCGGC
  CCTGGCAGCTTGCATCTGAAGAATGGGCCGGAGAGCATCATGAACTCAACGGAGTACCCA
  CACGGTGGCTCCCAGGGACAGCTGCTGCATCCTGGTGACCACAAGGCCCAGGCCCACAAC
  AAGGCACTGCCCCCAGGCCTGAGCAAGGAGCAGGCCCCTGACTGTAGCAGCAGTGATGGT
  TCTGAGAGGAGCTGGATGGATGACTACGATTACGTCCACCTACAGGGTAAGGAGGAGTTT
  GAGAGGCAACAGAAAGAGCTATTGGAAAAAGAGAATATCATGAAACAGAACAAGATGCAG
  CTGGAACATCATCAGCTGAGCCAGTTCCAGCTGTTGGAACAAGAGATTACAAAGCCCGTG
  GAGAATGACATCTCGAAGTGGAAGCCCTCTCAGAGCCTACCCACCACAAACAGTGGCGTG
  AGTGCTCAGGATCGGCAGTTGCTGTGCTTCTACTATGACCAATGTGAGACCCATTTCATT
  TCCCTTCTCAACGCCATTGACGCACTCTTCAGTTGTGTCAGCTCAGCCCAGCCCCCGCGA
  ATCTTCGTGGCACACAGCAAGTTTGTCATCCTCAGTGCACACAAACTGGTGTTCATTGGA
  GACACGCTGACACGGCAGGTGACTGCCCAGGACATTCGCAACAAAGTCATGAACTCCAGC
  AACCAGCTCTGCGAGCAGCTCAAGACTATAGTCATGGCAACCAAGATGGCCGCCCTCCAT
  TACCCCAGCACCACGGCCCTGCAGGAAATGGTGCACCAAGTGACAGACCTTTCTAGAAAT
  GCCCAGCTGTTCAAGCGCTCTTTGCTGGAGATGGCAACGTTCTGA
  
  Clone variation with respect to NM_006403.3
  141 a=>g;525 c=>t;1476 t=>c;2367 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006403 unedited
  TAGCTTTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGCGCTGCGACTG
  ACAAGCGGCTCTGCCCGGGACCTTCTCGCTTTCATCTAGCGCTGCACTCAATGGAGGGGC
  GGGCACCGCAGTGCTTAATGCTGTCTTAACTAGTGTAGGAAAACGGCTCAACCCACCGCT
  GCCGAAATGAAGTATAAGAATCTTATGGCAAGGGCCTTATATGACAATGTCCCAGAGTGT
  GCCGAGGAACTGGCCTTTCGCAAGGGAGACATCCTGACCGTCATAGAGCAGAACACAGGG
  GGACTGGAAGGATGGTGGCTGTGCTCGTTACACGGTCGGCAAGGCATTGTCCCAGGCAAC
  CGGGTGAAGCTTCTGATTGGTCCCATGCAGGAGACTGCCTCCAGTCACGAGCAGCCTGCC
  TCTGGACTGATGCAGCAGACCTTTGGCCAACAGAAGCTCTATCAAGTGCCAAACCCACAG
  GCTGCTCCCCGAGACACCATCTACCAAGTGCCACCTTCCTACCAAAATCAGGGAATTTAC
  CAAGTCCCCACTGGCCACGGCACCCAAGAACAAGAGGTATATCAGGTGCCACCATCAGTG
  CAGAGAAGCATTGGGGGAACCAGTGGGCCCCACGTGGGTAAAAAGGTGATAACCCNCGTG
  AGGACAGGCCATGGCTACGTATACGAGTACCCATCCAGATACCAAAAGGATGTCTATGAT
  ATCCCTCCTTCTCATACCACTCAAGGGGTATACGACATCCCTNCCTCATCAGCAAAGGCC
  CTGTGTTTTCAGTTCCAGTGGGGAGAGAAAAACCTCANGGGGGTGTATGACATNCCCGCC
  TACAAAGGGTATATGCCATTCCCGCCCTCTGCTGCCGGATGAAGCAGGNCTTAGGGAAAA
  GACTATGACTTCCCCCTCCATGAGAAGNCTGNAAGGCGACTCACCCGNGGGGGGGGTTTA
  GCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006403 unedited
  AGCTCTGGACCGGCGGCCGCATNCTAGNAGTCGGTTTTTTTTTTTTTTTTTTCAGAGACT
  AAAATTTAATGATTTAAAAATAGCCTCTACATACAAGATGGCAAAGAGTAATACAGATTG
  AACATTTGGTTTTAAAAATGTGAAATGTCTCCACTTAGCGTAGATCAATCAAGTCAGCCA
  TCTCCTAAGAAATACACATTATACAATGAAATCTACAAAGACACACTTTTTAACTTCAAG
  CGTTGTTGATTTTCAGCAACCCTCTTCCCACATGAACATTTCCTTGTAATGTAATGTATG
  ACTTTTAATCTTCTTTTGGCAGAGTAGGACTTTGAGAATTATAATAGCAGTTGTTTTGAA
  AAGCACCTTCTATGATCATTTATTTCCACTGATTTTTTTTTTAATCACGCTGAGATCATG
  CATTCTTGTTTTTTAAATCCTTTTCTGTTCCAATGATTTGGTTGTTTAAAACAAGCAAGC
  TTAATTCAGTCAGGCATATATTGCAAATAAAAACATGTTTCCAAGTAGGCTGGAATAAAA
  ATGCTAAATAAGAACTGGGCCAAAAGATCATAGATAATGTGCTTCTTCTCTTGGTAATAT
  GTAAATACATTTACCCATGGGGNTAGAAGTATTTCACCTGCCCCATGAAGAACCTGATGG
  CTTGTGGNNTGTCTGTGCTGTGTCAATACTAACAGATGTGAGGAGACAGACTATGGAATG
  GACGCTGTGACTATCTGTGAATGTGGGAAAGGGGGTTATACACACTGGCCACAGGAAAGT
  GCAAAATTAGATGGACTCTGTGTAGCTAGCCACTCTTGANTGTCAGGTCTGCATATGTGA
  GTTTTAAAGAAAGTGCAAGATAGCANTAAATTNCCTTTAGAGAGCAGGAATGTNCCTGGG
  AGCTCTATAGNTCCATGATCAAGGACCTGGCCAGTTTCCTTCAGTAATGGGGGGTAGTGC
  CACAAAGGGAATATTAGAGTCAAAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006403
• Insert Size: 4310 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006403.2, NP_006394.1
• RefSeq Size: 4532 bp
• RefSeq ORF: 2505 bp
• Locus ID: 4739
• Cytogenetics: 6p24.2
• Domains: SH3
• Gene Summary: 'The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]'
  Transcript Variant: This variant (1) encodes isoform 1.