HEF1 (NEDD9) (NM_006403) Human Untagged Clone

CAT#: SC116092

NEDD9 (untagged)-Human neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), transcript variant 1


  "NM_006403" in other vectors (6)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol NEDD9
Synonyms CAS-L; CAS2; CASL; CASS2; HEF1
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC116092 sequence for NM_006403 edited (data generated by NextGen Sequencing)
ATGAAGTATAAGAATCTTATGGCAAGGGCCTTATATGACAATGTCCCAGAGTGTGCCGAG
GAACTGGCCTTTCGCAAGGGAGACATCCTGACCGTCATAGAGCAGAACACAGGGGGACTG
GAAGGATGGTGGCTGTGCTCGTTACACGGTCGGCAAGGCATTGTCCCAGGCAACCGGGTG
AAGCTTCTGATTGGTCCCATGCAGGAGACTGCCTCCAGTCACGAGCAGCCTGCCTCTGGA
CTGATGCAGCAGACCTTTGGCCAACAGAAGCTCTATCAAGTGCCAAACCCACAGGCTGCT
CCCCGAGACACCATCTACCAAGTGCCACCTTCCTACCAAAATCAGGGAATTTACCAAGTC
CCCACTGGCCACGGCACCCAAGAACAAGAGGTATATCAGGTGCCACCATCAGTGCAGAGA
AGCATTGGGGGAACCAGTGGGCCCCACGTGGGTAAAAAGGTGATAACCCCCGTGAGGACA
GGCCATGGCTACGTATACGAGTACCCATCCAGATACCAAAAGGATGTCTATGATATCCCT
CCTTCTCATACCACTCAAGGGGTATACGACATCCCTCCCTCATCAGCAAAAGGCCCTGTG
TTTTCAGTTCCAGTGGGAGAGATAAAACCTCAAGGGGTGTATGACATCCCGCCTACAAAA
GGGGTATATGCCATTCCGCCCTCTGCTTGCCGGGATGAAGCAGGGCTTAGGGAAAAAGAC
TATGACTTCCCCCCTCCCATGAGACAAGCTGGAAGGCCGGACCTCAGACCGGAGGGGGTT
TATGACATTCCTCCAACCTGCACCAAGCCAGCAGGGAAGGACCTTCATGTAAAATACAAC
TGTGACATTCCAGGAGCTGCAGAACCGGTGGCTCGAAGGCACCAGAGCCTGTCCCCGAAT
CACCCACCCCCGCAACTCGGACAGTCAGTGGGCTCTCAGAACGACGCATATGATGTCCCC
CGAGGCGTTCAGTTTCTTGAGCCACCAGCAGAAACCAGTGAGAAAGCAAACCCCCAGGAA
AGGGATGGTGTTTATGATGTCCCTCTGCATAACCCGCCAGATGCTAAAGGCTCTCGGGAC
TTGGTGGATGGGATCAACCGATTGTCTTTCTCCAGTACAGGCAGCACCCGGAGTAACATG
TCCACGTCTTCCACCTCCTCCAAGGAGTCCTCACTGTCAGCCTCCCCAGCTCAGGACAAA
AGGCTCTTCCTGGATCCAGACACAGCTATTGAGAGACTTCAGCGGCTCCAGCAGGCCCTT
GAGATGGGTGTCTCCAGCCTAATGGCACTGGTCACTACCGACTGGCGGTGTTACGGATAT
ATGGAAAGACACATCAATGAAATACGCACAGCAGTGGACAAGGTGGAGCTGTTCCTGAAG
GAGTACCTCCACTTTGTCAAGGGAGCTGTTGCAAATGCTGCCTGCCTCCCGGAACTCATC
CTCCACAACAAGATGAAGCGGGAGCTGCAACGAGTCGAAGACTCCCACCAGATCCTGAGT
CAAACCAGCCATGACTTAAATGAGTGCAGCTGGTCCCTGAATATCTTGGCCATCAACAAG
CCCCAGAACAAGTGTGACGATCTGGACCGGTTTGTGATGGTGGCAAAGACGGTGCCCGAT
GACGCCAAGCAGCTCACCACAACCATCAACACCAACGCAGAGGCCCTCTTCAGACCCGGC
CCTGGCAGCTTGCATCTGAAGAATGGGCCGGAGAGCATCATGAACTCAACGGAGTACCCA
CACGGTGGCTCCCAGGGACAGCTGCTGCATCCTGGTGACCACAAGGCCCAGGCCCACAAC
AAGGCACTGCCCCCAGGCCTGAGCAAGGAGCAGGCCCCTGACTGTAGCAGCAGTGATGGT
TCTGAGAGGAGCTGGATGGATGACTACGATTACGTCCACCTACAGGGTAAGGAGGAGTTT
GAGAGGCAACAGAAAGAGCTATTGGAAAAAGAGAATATCATGAAACAGAACAAGATGCAG
CTGGAACATCATCAGCTGAGCCAGTTCCAGCTGTTGGAACAAGAGATTACAAAGCCCGTG
GAGAATGACATCTCGAAGTGGAAGCCCTCTCAGAGCCTACCCACCACAAACAGTGGCGTG
AGTGCTCAGGATCGGCAGTTGCTGTGCTTCTACTATGACCAATGTGAGACCCATTTCATT
TCCCTTCTCAACGCCATTGACGCACTCTTCAGTTGTGTCAGCTCAGCCCAGCCCCCGCGA
ATCTTCGTGGCACACAGCAAGTTTGTCATCCTCAGTGCACACAAACTGGTGTTCATTGGA
GACACGCTGACACGGCAGGTGACTGCCCAGGACATTCGCAACAAAGTCATGAACTCCAGC
AACCAGCTCTGCGAGCAGCTCAAGACTATAGTCATGGCAACCAAGATGGCCGCCCTCCAT
TACCCCAGCACCACGGCCCTGCAGGAAATGGTGCACCAAGTGACAGACCTTTCTAGAAAT
GCCCAGCTGTTCAAGCGCTCTTTGCTGGAGATGGCAACGTTCTGA

Clone variation with respect to NM_006403.3
141 a=>g;525 c=>t;1476 t=>c;2367 c=>t
>OriGene 5' read for NM_006403 unedited
TAGCTTTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGCGCTGCGACTG
ACAAGCGGCTCTGCCCGGGACCTTCTCGCTTTCATCTAGCGCTGCACTCAATGGAGGGGC
GGGCACCGCAGTGCTTAATGCTGTCTTAACTAGTGTAGGAAAACGGCTCAACCCACCGCT
GCCGAAATGAAGTATAAGAATCTTATGGCAAGGGCCTTATATGACAATGTCCCAGAGTGT
GCCGAGGAACTGGCCTTTCGCAAGGGAGACATCCTGACCGTCATAGAGCAGAACACAGGG
GGACTGGAAGGATGGTGGCTGTGCTCGTTACACGGTCGGCAAGGCATTGTCCCAGGCAAC
CGGGTGAAGCTTCTGATTGGTCCCATGCAGGAGACTGCCTCCAGTCACGAGCAGCCTGCC
TCTGGACTGATGCAGCAGACCTTTGGCCAACAGAAGCTCTATCAAGTGCCAAACCCACAG
GCTGCTCCCCGAGACACCATCTACCAAGTGCCACCTTCCTACCAAAATCAGGGAATTTAC
CAAGTCCCCACTGGCCACGGCACCCAAGAACAAGAGGTATATCAGGTGCCACCATCAGTG
CAGAGAAGCATTGGGGGAACCAGTGGGCCCCACGTGGGTAAAAAGGTGATAACCCNCGTG
AGGACAGGCCATGGCTACGTATACGAGTACCCATCCAGATACCAAAAGGATGTCTATGAT
ATCCCTCCTTCTCATACCACTCAAGGGGTATACGACATCCCTNCCTCATCAGCAAAGGCC
CTGTGTTTTCAGTTCCAGTGGGGAGAGAAAAACCTCANGGGGGTGTATGACATNCCCGCC
TACAAAGGGTATATGCCATTCCCGCCCTCTGCTGCCGGATGAAGCAGGNCTTAGGGAAAA
GACTATGACTTCCCCCTCCATGAGAAGNCTGNAAGGCGACTCACCCGNGGGGGGGGTTTA
GCT
>OriGene 3' read for NM_006403 unedited
AGCTCTGGACCGGCGGCCGCATNCTAGNAGTCGGTTTTTTTTTTTTTTTTTTCAGAGACT
AAAATTTAATGATTTAAAAATAGCCTCTACATACAAGATGGCAAAGAGTAATACAGATTG
AACATTTGGTTTTAAAAATGTGAAATGTCTCCACTTAGCGTAGATCAATCAAGTCAGCCA
TCTCCTAAGAAATACACATTATACAATGAAATCTACAAAGACACACTTTTTAACTTCAAG
CGTTGTTGATTTTCAGCAACCCTCTTCCCACATGAACATTTCCTTGTAATGTAATGTATG
ACTTTTAATCTTCTTTTGGCAGAGTAGGACTTTGAGAATTATAATAGCAGTTGTTTTGAA
AAGCACCTTCTATGATCATTTATTTCCACTGATTTTTTTTTTAATCACGCTGAGATCATG
CATTCTTGTTTTTTAAATCCTTTTCTGTTCCAATGATTTGGTTGTTTAAAACAAGCAAGC
TTAATTCAGTCAGGCATATATTGCAAATAAAAACATGTTTCCAAGTAGGCTGGAATAAAA
ATGCTAAATAAGAACTGGGCCAAAAGATCATAGATAATGTGCTTCTTCTCTTGGTAATAT
GTAAATACATTTACCCATGGGGNTAGAAGTATTTCACCTGCCCCATGAAGAACCTGATGG
CTTGTGGNNTGTCTGTGCTGTGTCAATACTAACAGATGTGAGGAGACAGACTATGGAATG
GACGCTGTGACTATCTGTGAATGTGGGAAAGGGGGTTATACACACTGGCCACAGGAAAGT
GCAAAATTAGATGGACTCTGTGTAGCTAGCCACTCTTGANTGTCAGGTCTGCATATGTGA
GTTTTAAAGAAAGTGCAAGATAGCANTAAATTNCCTTTAGAGAGCAGGAATGTNCCTGGG
AGCTCTATAGNTCCATGATCAAGGACCTGGCCAGTTTCCTTCAGTAATGGGGGGTAGTGC
CACAAAGGGAATATTAGAGTCAAAAG
Restriction Sites NotI-NotI     
ACCN NM_006403
Insert Size 4310 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006403.2, NP_006394.1
RefSeq Size 4532 bp
RefSeq ORF 2505 bp
Locus ID 4739
Cytogenetics 6p24.2
Domains SH3
Gene Summary 'The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]'
Transcript Variant: This variant (1) encodes isoform 1.

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