RRAS (NM_006270) Human Untagged Clone

CAT#: SC116180

RRAS (untagged)-Human related RAS viral (r-ras) oncogene homolog (RRAS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RRAS
• Synonyms: R-Ras
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116180 sequence for NM_006270 edited (data generated by NextGen Sequencing)
  ATGAGCAGCGGGGCGGCGTCCGGGACAGGGCGGGGGCGGCCCCGGGGCGGGGGACCTGGG
  CCCGGGGACCCCCCGCCCAGCGAGACACACAAGCTGGTGGTCGTGGGCGGCGGCGGCGTG
  GGCAAGAGCGCGCTGACCATCCAGTTCATCCAGTCCTACTTCGTGTCTGACTACGACCCC
  ACTATTGAGGACTCCTACACGAAGATCTGCAGTGTGGATGGCATCCCAGCCCGGCTGGAC
  ATCCTGGACACCGCGGGCCAGGAAGAGTTCGGGGCCATGAGAGAGCAGTACATGCGTGCT
  GGCCACGGCTTCCTGCTGGTGTTCGCCATTAATGACCGGCAGAGTTTCAACGAGGTGGGC
  AAGCTCTTCACGCAGATTCTGCGGGTCAAGGACCGCGACGACTTCCCCGTTGTGTTGGTC
  GGGAACAAGGCAGATCTGGAGTCACAGCGCCAGGTCCCCCGATCAGAAGCCTCTGCCTTC
  GGCGCCTCCCACCACGTGGCCTACTTTGAGGCCTCGGCCAAACTGCGTCTCAACGTGGAC
  GAGGCTTTTGAGCAGCTGGTGCGGGCTGTCCGGAAATACCAGGAACAAGAGCTCCCACCG
  AGCCCTCCCAGTGCCCCCAGGAAGAAGGGCGGGGGCTGCCCCTGCGTCCTCCTGTAG
  
  Clone variation with respect to NM_006270.3
  333 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006270 unedited
  TTGGATTTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACCAGGCGGTGGCGACA
  TGAGCAGCGGGGCGGCGTCCGGGACAGGGCGGGGGCGGCCCCGGGGCGGGGGACCTGGGC
  CCGGGGACCCCCCGCCCAGCGAGACACACAAGCTGGTGGTCGTGGGCGGCGGCGGCGTGG
  GCAAGAGCGCGCTGACCATCCAGTTCATCCAGTCCTACTTCGTGTCTGACTACGACCCCA
  CTATTGAGGACTCCTACACGAAGATCTGCAGTGTGGATGGCATCCCAGCCCGGCTGGACA
  TCCTGGACACCGCGGGCCAGGAAGAGTTCGGGGCCATGAGAGAGCAGTACATGCGTGCTG
  GCCACGGCTTCCTGCTGGTGTTCGCCATTAATGACCGGCAGAGTTTCAACGAGGTGGGCA
  AGCTCTTCACGCAGATTCTGCGGGTCAAGGACCGCGACGACTTCCCCGTTGTGTTGGTCG
  GGAACAAGGCAGATCTGGAGTCACAGCGCCAGGTCCCCCGATCAGAAGCCTCTGCCTTCG
  GCGCCTCCCACCACGTGGCCTACTTTGAGGCCTCGGCCAAACTGCGTCTCAACGTGGACG
  AGGCTTTTGAGCAGCTGGTGCGGGCTGTCCGGAAATACCAGGAACAAGAGCTCCCACCGA
  GCCCTCCCAGTGCCCCCAGGAAGAAGGGCGGNGGCTGCCCCTGCGTCCTCCTGTAGCCCA
  GGCAAGAGAGAAGCAACCACCACAAGCTCTCGGGACTAGCTGCCTTCGCACCTTGCTGTG
  TGACCTGAGGCCCTCACTGAGCCTCAATTTTCTCATCTGGGTCTCCCAGGACACATCACA
  TACCCACCTTTACTTNCTGGCCTCTNCTGGGCTACTGCCACTGTGTGCCTTCTGCCAACG
  CCTCCTGTCCCCACCTAACCTGTGGNGGGTGAGGGGCTCCCGGTCACTGCTGAATTAACT
  CCCCTNCCCAGAAATAAAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006270 unedited
  TTTTTTTTTGACGTTGGCAGTGACATTTATTTTTCTGGGGGAGGGGAGTTATATACAGCA
  GTGACCCGGAGCCCCTCACCCCCACCAGGCTTAGGTGGGGACAGGAGGCGTTGGCAGAAG
  GCACACAGTGGCAGTAGCCCAGAAGAGGCCAGGAAGTAAGGGTGGGTATGTGATGTGTCC
  TGGGAGACCCAGATGAGGAAATTGAGGCTCAGTGAGGGCCTCAGGTCACACAACAAGGTG
  CGAAGGCAGCTAGTCCCGAGAGCTTGTGGTGGTTGCTTCTCTCTTGCCTGGGCTACAGGA
  GGACGCAGGGGCAGCCCCCGCCCTTCTTCCTGGGGGCACTGGGAGGGCTCGGTGGGAGCT
  CTTGTTCCTGGTATTTCCGGACAGCCCGCACCAGCTGCTCAAAAGCCTCGTCCACGTTGA
  GACGCAGTTTGGCCGAGGCCTCAAAGTAGGCCACGTGGTGGGAGGCGCCGAAGGCAGAGG
  CTTCTGATCGGGGGACCTGGCGCTGTGACTCCAGATCTGCCTTGTTCCCGACCAACACAA
  CGGGGAAGTCGTCGCGGTCCTTGACCCGCAGAATCTGCGTGAAGAGCTTGCCCACCTCGT
  TGAAACTCTGCCGGTCATTAATGGCGAACACCAGCAGGAAGCCGTGGCCAGCACGCATGT
  AC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006270
• Insert Size: 1100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006270.2, NP_006261.1
• RefSeq Size: 1017 bp
• RefSeq ORF: 657 bp
• Locus ID: 6237
• Cytogenetics: 19q13.33
• Domains: ras, RAN, RAS, RHO, RAB
• Protein Families: Druggable Genome
• Protein Pathways: MAPK signaling pathway, Regulation of actin cytoskeleton, Tight junction
• Gene Summary: 'The protein encoded by this gene is a small GTPase involved in diverse processes including angiogenesis, vascular homeostasis and regeneration, cell adhesion, and neuronal axon guidance. Mutations in this gene are found in many invasive cancers. [provided by RefSeq, Jul 2015]'