Nociceptin (PNOC) (NM_006228) Human Untagged Clone

CAT#: SC116235

PNOC (untagged)-Human prepronociceptin (PNOC)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PNOC
• Synonyms: N/OFQ; NOP; OFQ; ppN/OFQ; PPNOC
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116235 sequence for NM_006228 edited (data generated by NextGen Sequencing)
  ATGAAAGTCCTGCTTTGTGACCTGCTGCTGCTCAGTCTCTTCTCCAGTGTGTTCAGCAGT
  TGTCAGAGGGACTGTCTCACATGCCAGGAGAAGCTCCACCCAGCCCTGGACAGCTTCGAC
  CTGGAGGTGTGCATCCTCGAGTGTGAAGAGAAGGTCTTCCCCAGCCCCCTCTGGACTCCA
  TGCACCAAGGTCATGGCCAGGAGCTCTTGGCAGCTCAGCCCTGCCGCCCCAGAGCATGTG
  GCGGCTGCTCTCTACCAGCCGAGAGCTTCGGAGATGCAGCATCTGCGGCGAATGCCCCGA
  GTCCGGAGCTTGTTCCAGGAGCAGGAAGAGCCCGAGCCTGGCATGGAGGAGGCTGGTGAG
  ATGGAGCAGAAGCAGCTGCAGAAGAGATTTGGGGGCTTCACCGGGGCCCGGAAGTCGGCC
  AGGAAGTTGGCCAATCAGAAGCGGTTCAGTGAGTTTATGAGGCAATACTTGGTCCTGAGC
  ATGCAGTCCAGCCAGCGCCGGCGCACCCTGCACCAGAATGGTAATGTGTAG
  
  Clone variation with respect to NM_006228.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006228 unedited
  NGGGCACCATTTGTATACGACTCCTATAGGCGGCCCGCGAATTCGCACGAGGCCTCGTGC
  CGAATTCGGCACGAGGATCGGAGGGAGCCGGGGACTGACAGCTCTCAGCACCTGCTTCCT
  GCTCCTGCACCATGAAAGTCCTGCTTTGTGACCTGCTGCTGCTCAGTCTCTTCTCCAGTG
  TGTTCAGCAGTTGTCAGAGGGACTGTCTCACATGCCAGGAGAAGCTCCACCCAGCCCTGG
  ACAGCTTCGACCTGGAGGTGTGCATCCTCGAGTGTGAAGAGAAGGTCTTCCCCAGCCCCC
  TCTGGACTCCATGCACCAAGGTCATGGCCAGGAGCTCTTGGCAGCTCAGCCCTGCCGCCC
  CAGAGCATGTGGCGGCTGCTCTCTACCAGCCGAGAGCTTCGGAGATGCAGCATCTGCGGC
  GAATGCCCCGAGTCCGGAGCTTGTTCCAGGAGCAGGAAGAGCCCGAGCCTGGCATGGAGG
  AGGCTGGTGAGATGGAGCAGAAGCAGCTGCAGAAGAGATTTGGGGGCTTCACCGGGGCCC
  GGAAGTCGGCCAGGAAGTTGGCCAATCAGAAGCGGTTCAGTGAGTTTATGAGGCAATACT
  TGGTCCTGAGCATGCAGTCCAGCCAGCGCCGGCGCACCCTGCACCAGAATGGTAATGTGT
  AGCCGGAAGGGGCGCTCCTCCCAGCTGTACCGGCCACTGCAACCCATGAGCGTTCAGGTG
  ATCCCCCAACAGCATGTGCTCAGCCCCAGACCTGCCGCCTGGGAATCAGGATTCCTTTTT
  TCCCCAAGCCACTGAGCGCCTGCAGATTCCGCCAGGCCTTGTTTGCCTCCANAACCTTCC
  CTATTGATTGTTTCTCCCCACCCCCTTGCATGTTTACCACAAACCTGTTGCTACATAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006228 unedited
  GCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTAACCATTTGCTCAGTAGAAGTTTA
  ATGGAGAAATCGTTGTTTAAAACAATCAGTCAAAAAGAACAGCTCTTTTACAAACAAGTT
  ATGGCAGTGGCAAGTCAAAACCCCAGGTTCAATTTCCTATTCCTTTCACCTGCCCCTAGA
  AGGGGCAAGAGGCGGGTGAGCAGGAGAGATGGGGCTATTGAAATGGTAGCTAGAGGAATT
  ACAAAAATACACTCTGATGTAGCAACAGGGTTGTGGTGAAACATGCCAGGGGGCTGGGGA
  GGAACAATCAGACGGGAAGGTTCTGGAGGCAAACGAAGCCTGCGGGATCTGCAGGCGCTC
  AGTGCCTTGGGGAAGAAGGAATCCTGATTCCCAGGCGGCAGGTCTGGGGCTGAGCACATG
  CTGTTTGGGGGATCACCTGGACGCTCATGGGTTGCAGTGGCCGGTACAGCTGGGAGGAGC
  GCCCCTTCCGGCTACACATTACCATTCTGGTGCAGGGTGCGCCGGCGCTGGCTGGACTGC
  ATGCTCAGGACCAAGTATTGCCTCATAAACTCACTGAACCGCTTCTGATTGGCCAACTTC
  CTGGCCGACTTCCGGGCCCCGGTGAAGCCCCCAAATCTCTTCTGCAGCTGCTTCTGCTCC
  ATCTCACCAGCCTNCTCCATGCCCAGCTCGGGCTCTTCCTGCTCCTGGAAAACAGCTCNG
  ACTCGGGGCATCTCGCGCAGATGCTGCATCTCCGAAACTCTCGGCTGTAAAAGACAACCG
  CCACATGCTCTTGGGCGGCAAGGCTGACCTCCAAGACCTCCTGGCCTGACCTTGGGCCAT
  GAGCCAAAGGGGCCTGGGAAAACCTCTCTTCCACTTGAGATGCCACCCCCAGTCAAACTG
  CCACAGCTGGGGGGACTTCTCCGGCCGGTAACACGCCCTTTGACACGGNTGAACCTGGAA
  AAAAACGTCCCACCCGTCCAACCGGCCTTCTTGGGCGACG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006228
• Insert Size: 1110 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006228.2, NP_006219.1
• RefSeq Size: 1198 bp
• RefSeq ORF: 531 bp
• Locus ID: 5368
• Cytogenetics: 8p21.1
• Domains: Opiods_neuropep
• Protein Families: Secreted Protein
• Gene Summary: 'This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include nociceptin, nocistatin, and orphanin FQ2 (OFQ2). Nociceptin, also known as orphanin FQ, is a 17-amino acid neuropeptide that binds to the nociceptin receptor to induce increased pain sensitivity, and may additionally regulate body temperature, learning and memory, and hunger. Another product of the encoded preproprotein, nocistatin, may inhibit the effects of nociceptin. [provided by RefSeq, Jul 2015]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.