RNA Polymerase II p14.5 (POLR2I) (NM_006233) Human Untagged Clone

CAT#: SC116236

POLR2I (untagged)-Human polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa (POLR2I)


  "NM_006233" in other vectors (4)

Reconstitution Protocol

USD 420.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol POLR2I
Synonyms hRPB14.5; RPB9
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_006233, the custom clone sequence may differ by one or more nucleotides


ATGGAGCCCGACGGGACTTACGAGCCGGGCTTCGTGGGTATTCGCTTCTGCCAGGAATGTAACAACATGC
TGTACCCCAAGGAAGACAAGGAGAACCGCATTCTGCTCTACGCGTGCCGGAACTGTGATTACCAGCAGGA
GGCCGACAACAGCTGCATCTATGTCAACAAGATCACGCACGAAGTGGACGAACTGACCCAGATTATCGCC
GACGTGTCCCAGGACCCCACGTTGCCGCGGACCGAGGACCACCCGTGCCAAAAGTGCGGCCACAAGGAGG
CTGTGTTCTTCCAGTCACACAGTGCGCGGGCCGAGGACGCCATGCGCCTTTACTACGTGTGCACAGCCCC
ACACTGCGGCCACCGCTGGACCGAGTGA


>OriGene 5' read for NM_006233 unedited
GGGGTCAAAATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACCAGGAGGGCT
GCGCGGGCTGCGCGTCGCCATGGAGCCCGACGGGACTTACGAGCCGGGCTTCGTGGGTAT
TCGCTTCTGCCAGGAATGTGCCGGAACTGTGATTACCAGCAGGAGGCCGACAACAGCTGC
ATCTATGTCAACAAGATCACGCACGAAGTGGACGAACTGACCCAGATTATCGCCGACGTG
TCCCAGGACCCCACGTTGCCGCGGACCGAGGACCACCCGTGCCAAAAGTGCGGCCACAAG
GAGGCTGTGTTCTTCCAGTCACACAGTGCGCGGGCCGAGGACGCCATGCGCCTTTACTAC
GTGTGCACAGCCCCACACTGCGGCCACCGCTGGACCGAGTGACCTCCTCTCTCCCCCGAG
TGTAATAAACACCAGATTCCACANNCAAAAAAACCACCCCCCACTCAAAAAAAAAAACAA
CTAACAAACCCCTCGACTCTAGATTGCGGCCGCGGCCATAGCTGTTTCCTGAACAGATCC
CGGTGGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCC
AGGGCCCACCATCCCTTGCCCTAATAAAATTAAATTGCATCATTTTGTCTGACTAGGTGC
CCTTCTATAATATTATGGGGTGGAGGGGGGGGGGGAATGGTATTTATGCCCTGATTCTCA
GAACCAAATACCATGACACCCTACCGCCAACACATACCCCATCAGAAAATATAGCACTTT
ACGAACCACACTAAACCACAACGATACTTCAATAAAATATATTCCATATGAATTCCACAA
CTCTATCCTCACACCGAGCCAACACACCACCCCCACCCTCAACCACCCACACCCACAATC
CCTACTNAACAAACTCAAAANAACAACCCCAANAATC
>OriGene 3' read for NM_006233 unedited
ACTTGTCCGCGGCACGCAATCTAGATCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTTGGGAAACCGGGGGTTTTTACACTCCGGGGGAAAAAAGAAG
GCACTTCGTCCAACGGGGGCCCCAATGTGGGGCTTTGCCCACCTTTTAAAAGGGCATGGG
GTCCTTTGCCCCCGCCCTTTGTGAATGGAAAAAACAACCCCCTTTTGGCCCCACTTTTTG
CACGGGTGGGCCTCGGTCCCCGGGAACCTGGGGGCCTGGGACACCTTGGGGATAATTTGG
GGCAATTCCTCCACTTTGGGCCGGAACTTTTTGACATAAATGCAACTGTTGTCGGCCCCC
TTCTGGTAATCACAATTCCCGGCCATTTCTGGCAAAAACGAATTCCCCCCAAACCCCGCT
CTTAAATCCCCTCCGGCTCCCTTGGGAACCCCAATCCCCCCATACCTTCTGGTGCCCAAT
TTCCCGGCGCCCCTATAAGAGACCCATTAACAAAATCTGAACGGTACCTTAAAAAACCCT
TCCTTTTTTAAACCTCCACCCGTCACCCTTTACCCCTTATTTTTGTTCCCCGCCCGGGTT
TTTACCAACCTTTGAAAACATCCCTTTTTTTCGGCCCCAAAACAACCTCCCCACTCCTCA
AATGGGGGCGCAATTTCAAAACCCTCGAGTCAAACCCCCCTTTCCCCTCTCTTCGTTTCT
TCTCCAAACCCACTACACCCCCGCAAACACCCACCCCCCATACCATAAACACCTTTAATT
AAAAAATCTCCCCCATATCTCCCTCCATATCTTTATCCCACACCGTTCATCCTTCTTTTT
ATATTTTTTGCAGTGGTATTTTCCCTACACCACCTCTTCATTCCCCTCCTCACCTCTTAT
CTCTCTCCCCCACCCCATCTTCCACCCCCACTACTTCCCTTCATGCCTCTCTCTCTCCAC
CTCTCCTG
Restriction Sites NotI-NotI     
ACCN NM_006233
Insert Size 450 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006233.4, NP_006224.1
RefSeq Size 885 bp
RefSeq ORF 378 bp
Locus ID 5438
Cytogenetics 19q13.12
Domains TFIIS, RNA_POL_M_15KD
Protein Families Transcription Factors
Protein Pathways Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
Gene Summary 'This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. [provided by RefSeq, Jul 2008]'

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