RNA Polymerase II p14.5 (POLR2I) (NM_006233) Human Untagged Clone

CAT#: SC116236

POLR2I (untagged)-Human polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa (POLR2I)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: POLR2I
• Synonyms: hRPB14.5; RPB9
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006233, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGCCCGACGGGACTTACGAGCCGGGCTTCGTGGGTATTCGCTTCTGCCAGGAATGTAACAACATGC
  TGTACCCCAAGGAAGACAAGGAGAACCGCATTCTGCTCTACGCGTGCCGGAACTGTGATTACCAGCAGGA
  GGCCGACAACAGCTGCATCTATGTCAACAAGATCACGCACGAAGTGGACGAACTGACCCAGATTATCGCC
  GACGTGTCCCAGGACCCCACGTTGCCGCGGACCGAGGACCACCCGTGCCAAAAGTGCGGCCACAAGGAGG
  CTGTGTTCTTCCAGTCACACAGTGCGCGGGCCGAGGACGCCATGCGCCTTTACTACGTGTGCACAGCCCC
  ACACTGCGGCCACCGCTGGACCGAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006233 unedited
  GGGGTCAAAATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACCAGGAGGGCT
  GCGCGGGCTGCGCGTCGCCATGGAGCCCGACGGGACTTACGAGCCGGGCTTCGTGGGTAT
  TCGCTTCTGCCAGGAATGTGCCGGAACTGTGATTACCAGCAGGAGGCCGACAACAGCTGC
  ATCTATGTCAACAAGATCACGCACGAAGTGGACGAACTGACCCAGATTATCGCCGACGTG
  TCCCAGGACCCCACGTTGCCGCGGACCGAGGACCACCCGTGCCAAAAGTGCGGCCACAAG
  GAGGCTGTGTTCTTCCAGTCACACAGTGCGCGGGCCGAGGACGCCATGCGCCTTTACTAC
  GTGTGCACAGCCCCACACTGCGGCCACCGCTGGACCGAGTGACCTCCTCTCTCCCCCGAG
  TGTAATAAACACCAGATTCCACANNCAAAAAAACCACCCCCCACTCAAAAAAAAAAACAA
  CTAACAAACCCCTCGACTCTAGATTGCGGCCGCGGCCATAGCTGTTTCCTGAACAGATCC
  CGGTGGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCC
  AGGGCCCACCATCCCTTGCCCTAATAAAATTAAATTGCATCATTTTGTCTGACTAGGTGC
  CCTTCTATAATATTATGGGGTGGAGGGGGGGGGGGAATGGTATTTATGCCCTGATTCTCA
  GAACCAAATACCATGACACCCTACCGCCAACACATACCCCATCAGAAAATATAGCACTTT
  ACGAACCACACTAAACCACAACGATACTTCAATAAAATATATTCCATATGAATTCCACAA
  CTCTATCCTCACACCGAGCCAACACACCACCCCCACCCTCAACCACCCACACCCACAATC
  CCTACTNAACAAACTCAAAANAACAACCCCAANAATC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006233 unedited
  ACTTGTCCGCGGCACGCAATCTAGATCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTGGGAAACCGGGGGTTTTTACACTCCGGGGGAAAAAAGAAG
  GCACTTCGTCCAACGGGGGCCCCAATGTGGGGCTTTGCCCACCTTTTAAAAGGGCATGGG
  GTCCTTTGCCCCCGCCCTTTGTGAATGGAAAAAACAACCCCCTTTTGGCCCCACTTTTTG
  CACGGGTGGGCCTCGGTCCCCGGGAACCTGGGGGCCTGGGACACCTTGGGGATAATTTGG
  GGCAATTCCTCCACTTTGGGCCGGAACTTTTTGACATAAATGCAACTGTTGTCGGCCCCC
  TTCTGGTAATCACAATTCCCGGCCATTTCTGGCAAAAACGAATTCCCCCCAAACCCCGCT
  CTTAAATCCCCTCCGGCTCCCTTGGGAACCCCAATCCCCCCATACCTTCTGGTGCCCAAT
  TTCCCGGCGCCCCTATAAGAGACCCATTAACAAAATCTGAACGGTACCTTAAAAAACCCT
  TCCTTTTTTAAACCTCCACCCGTCACCCTTTACCCCTTATTTTTGTTCCCCGCCCGGGTT
  TTTACCAACCTTTGAAAACATCCCTTTTTTTCGGCCCCAAAACAACCTCCCCACTCCTCA
  AATGGGGGCGCAATTTCAAAACCCTCGAGTCAAACCCCCCTTTCCCCTCTCTTCGTTTCT
  TCTCCAAACCCACTACACCCCCGCAAACACCCACCCCCCATACCATAAACACCTTTAATT
  AAAAAATCTCCCCCATATCTCCCTCCATATCTTTATCCCACACCGTTCATCCTTCTTTTT
  ATATTTTTTGCAGTGGTATTTTCCCTACACCACCTCTTCATTCCCCTCCTCACCTCTTAT
  CTCTCTCCCCCACCCCATCTTCCACCCCCACTACTTCCCTTCATGCCTCTCTCTCTCCAC
  CTCTCCTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006233
• Insert Size: 450 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006233.4, NP_006224.1
• RefSeq Size: 885 bp
• RefSeq ORF: 378 bp
• Locus ID: 5438
• Cytogenetics: 19q13.12
• Domains: TFIIS, RNA_POL_M_15KD
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Gene Summary: 'This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. [provided by RefSeq, Jul 2008]'