IPP 2 (PPP1R2) (NM_006241) Human Untagged Clone

CAT#: SC116238

PPP1R2 (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 2 (PPP1R2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP1R2
• Synonyms: IPP-2; IPP2; PPP1R2A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116238 sequence for NM_006241 edited (data generated by NextGen Sequencing)
  ATGGCGGCCTCGACGGCCTCGCACCGGCCCATCAAGGGGATCTTGAAGAACAAGACCTCT
  ACGACTTCCTCTATGGTGGCGTCGGCCGAACAGCCCCGCGGGAATGTCGACGAGGAGCTG
  AGCAAAAAATCCCAGAAGTGGGATGAAATGAACATCTTGGCGACGTATCATCCAGCAGAC
  AAAGACTATGGTTTAATGAAAATAGATGAACCAAGCACTCCTTACCATAGTATGATGGGG
  GATGATGAAGATGCCTGTAGTGACACCGAGGCCACTGAAGCCATGGCGCCAGACATCTTA
  GCCAGGAAATTAGCTGCAGCTGAAGGCTTGGAGCCAAAGTATCGGATTCAGGAACAAGAA
  AGCAGTGGAGAGGAGGATAGTGACCTCTCACCTGAAGAACGAGAAAAAAAGCGACAATTT
  GAAATGAAAAGGAAGCTTCACTACAATGAAGGACTCAATATCAAACTAGCCAGACAATTA
  ATTTCAAAAGACCTACATGATGATGATGAAGATGAAGAAATGTTAGAGACTGCAGATGGA
  GAAAGCATGAATACGGAAGAATCAAATCAAGGATCTACTCCAAGTGACCAACAGCAAAAC
  AAATTACGAAGTTCATAG
  
  Clone variation with respect to NM_006241.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006241 unedited
  CAGAATTTTGTAATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGGGTTCGGCG
  TTGGCTCTGGCGTCGGGGTCGTTGTGTCGTGACAACCGCTGCGGTAGCCGTTTCCGAGGC
  AGCAGTTGCGGCCGCTTTAGCCCTGAGCGGGATCTGCGGCTGCCTGCGAGTCTCTGCTGT
  GCCGACCCTTCTCTTCGCGGACCCCACGCCAAGCAGCGACCCTGAGCCGACAGCCGGAGC
  GCCCGGCAATGGCGGCCTCGACGGCCTCGCACCGGCCCATCAAGGGGATCTTGAAGAACA
  AGACCTCTACGACTTCCTCTATGGTGGCGTCGGCCGAACAGCCCCGCGGGAATGTCGACG
  AGGAGCTGAGCAAAAAATCCCAGAAGTGGGATGAAATGAACATCTTGGCGACGTATCATC
  CAGCAGACAAAGACTATGGTTTAATGAAAATAGATGAACCAAGCACTCCTTACCATAGTA
  TGATGGGGGATGATGAAGATGCCTGTAGTGACACCGAGGCCACTGAAGCCATGGCGCCAG
  ACATCTTAGCCAGGAAATTAGCTGCAGCTGAAGGCTTGGAGCCAAAGTATCGGATTCAGG
  AACAAGANAGCAGTGGAGAGGAGGATAGTGACCTCTCACCTGAAGAACGAGAAAAAAAAG
  CGACATTTGAAATGAAAAGGAAGCTTCACTACAATGAAGGACTCAATATCAAACTAGCCA
  GACATTAATTTCAAAGACCTCATGATGATGATGAAGATGAAGAAATGGTANAGACTGCAG
  ATGGAGAAAGCATGAATACGGNAGAATCAAATCAAGGATCTACTCCAAGTGACCAACAGC
  ANNNACATTTACGAAGNTCATAGACCGAGATTTGNTCACACTGNCAAATGTTGNTAGAGT
  AAAACCTGGACTATAGTACCGTGCTTNCTGGTCTTACAATCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006241 unedited
  CGCCCCTANAGNATACGAGTTAAATTCCCTTTTTTTTTTGGCATATTATTGTATTTTATT
  ACAGTACGTGTAGTGTATACTATACTGGAAAGGGGAAAAATGTAAACAAAACCAAAGTCA
  TGGGGAAAATGGCATCTTGCTTTAATCTTCAACTGAAAGTTACCTTAACAATCCATTTAC
  ACCATTATGTCAAATTTTAGTCATCTCTGCTTATTTTTAGACAAATGAAAACAGACAAGG
  TAACACCAAATAGTTAAGCACAGAAAGTGATACTGATTAAAAAGTTGAAAGTAAAATCTA
  CCTTGGCTAGAACTGAACATTCAGATCTGTCTCTCCAGAGGAAAATCTAACTTGAATCAT
  AATGGTTCATATTTTGACTAGTTCATACCCTATCAATTACCTACTTATGACTTGAACATA
  CCTTTTCTCAGCTGCATGTGACAGCCTAAAACCCCACTGAGCATGCTGCTTGGCATGCCT
  TACTCCTCTGAAATCATCATCTACTTTCTAAAAACCAGAAAATGAGTTTGCGATTTAAAT
  TTCAAAAAATAGTTTGCAGAAAACACAAAAAGAATCAACTGTTTAAAGTCTTATCCTTTT
  CTTCACTCTATGACAGCTACTTCTACAAAAAAAAAAAAAGAGTATGTGGGTACTTTCTAA
  GACTCAGAACAAGAAACCAAATCAGAGGTGCATGATATATGTGCCGCACATGTACAGACT
  TAATCTCTACATCCCCCAAGACAGATTTAAACAGGGGTATCCCACATTTAAATTCAGATA
  CAGAGACAACAGTTTTGTTCTAAATCANGGGATTTCTAGCTGAATGTTAAATACTGACTA
  CAGTTCCCAGGACTCTGATATTATCTCATTCAAAATCAAATAATGGCCCTCCCTCTACAA
  CAGGATTCTCCACTTTTATTATTTCAAACTGCGTTCCCATACCTGCAGGGGAGAAACCCG
  TCCATAAAACCTTCTCCATATGAATTATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006241
• Insert Size: 3270 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006241.3, NP_006232.1
• RefSeq Size: 3346 bp
• RefSeq ORF: 618 bp
• Locus ID: 5504
• Cytogenetics: 3q29
• Domains: IPP-2
• Protein Families: Druggable Genome
• Gene Summary: 'Protein phosphatase-1 (PP1) is one of the main eukaryotic serine/threonine phosphatases. The protein encoded by this gene binds to the catalytic subunit of PP1, strongly inhibiting its activity. Ten related pseudogenes have been found throughout the human genome. Several splice variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]'
  Transcript Variant: This variant (2) uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.