PKC eta (PRKCH) (NM_006255) Human Untagged Clone

CAT#: SC116250

PRKCH (untagged)-Human protein kinase C, eta (PRKCH)


  "NM_006255" in other vectors (7)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PRKCH
Synonyms nPKC-eta; PKC-L; PKCL; PRKCL
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_006255 edited
GCGGCCGCGAATTCGGCACGAGGCCCGGTTCTCCCGCTGCGAAGCAGCGCGGCCCCCCGG
GGCCGGGGCAGCGGCGCCGGCATGTCGTCTGGCACCATGAAGTTCAATGGCTATTTGAGG
GTCCGCATCGGTGAGGCAGTGGGGCTGCAGCCCACCCGCTGGTCCCTGCGCCACTCGCTC
TTCAAGAAGGGCCACCAGCTGCTGGACCCCTATCTGACGGTGAGCGTGGACCAGGTGCGC
GTGGGCCAGACCAGCACCAAGCAGAAGACCAACAAACCCACGTACAACGAGGAGTTTTGC
GCTAACGTCACCGACGGCGGCCACCTCGAGTTGGCCGTCTTCCACGAGACGCCCCTGGGC
TACGACCACTTCGTGGCCAACTGCACCCTGCAGTTCCAGGAGCTGCTGCGCACGACCGGC
GCCTCGGACACCTTCGAGGGTTGGGTGGATCTCGAGCCAGAGGGGAAAGTATTTGTGGTA
ATAACCCTTACCGGGAGTTTCACTGAAGCTACTCTCCAGAGAGACCGGATCTTCAAACAT
TTTACCAGGAAGCGCCAAAGGGCTATGCGAAGGCGAGTCCACCAGATCAATGGACACAAG
TTCATGGCCACGTATCTGAGGCAGCCCACCTACTGCTCTCACTGCAGGGAGTTTATCTGG
GGAGTGTTTGGGAAACAGGGTTATCAGTGCCAAGTGTGCACCTGTGTCGTCCATAAACGC
TGCCATCATCTAATTGTTACAGCCTGTACTTGCCAAAACAATATTAACAAAGTGGATTCA
AAGATTGCAGAACAGAGGTTCGGGATCAACATCCCACACAAGTTCAGCATCCACAACTAC
AAAGTGCCAACATTCTGCGATCACTGTGGCTCACTGCTCTGGGGAATAATGCGACAAGGA
CTTCAGTGTAAAATATGTAAAATGAATGTGCATATTCGATGTCAAGCGAACGTGGCCCCT
AACTGTGGGGTAAATGCGGTGGAACTTGCCAAGACCCTGGCAGGGATGGGTCTCCAACCC
GGAAATATTTCTCCAACCTCGAAACTCGTTTCCAGATCGACCCTAAGACGACAGGGAAAG
GAGAGCAGCAAAGAAGGAAATGGGATTGGGGTTAATTCTTCCAACCGACTTGGTATCGAC
AACTTTGAGTTCATCCGAGTGTTGGGGAAGGGGAGTTTTGGGAAGGTGATGCTTGCAAGA
GTAAAAGAAACAGGAGACCTCTATGCTGTGAAGGTGCTGAAGAAGGACGTGATTCTGCAG
GATGATGATGTGGAATGCACCATGACCGAGAAAAGGATCCTGTCTCTGGCCCGCAATCAC
CCCTTCCTCACTCAGTTGTTCTGCTGCTTTCAGACCCCCGATCGTCTGTTTTTTGTGATG
GAGTTTGTGAATGGGGGTGACTTGATGTTCCACATTCAGAAGTCTCGTCGTTTTGATGAA
GCACGAGCTCGCTTCTATGCTGCAGAAATCATTTCGGCTCTCATGTTCCTCCATGATAAA
GGAATCATCTATAGAGATCTGAAACTGGACAATGTCCTGTTGGACCACGAGGGTCACTGT
AAACTGGCAGACTTCGGAATGTGCAAGGAGGGGATTTGCAATGGTGTCACCACGGCCACA
TTCTGTGGCACGCCAGACTATATCGCTCCAGAGATCCTCCAGGAAATGCTGTACGGGCCT
GCAGTAGACTGGTGGGCAATGGGCGTGTTGCTCTATGAGATGCTCTGTGGTCACGCGCCT
TTTGAGGCAGAGAATGAAGATGACCTCTTTGAGGCCATACTGAATGATGAGGTGGTCTAC
CCTACCTGGCTCCATGAAGATGCCACAGGGATCCTAAAATCTTTCATGACCAAGAACCCC
ACCATGCGCTTGGGCAGCCTGACTCAGGGAGGCGAGCACGCCATCTTGAGACATCCTTTT
TTTAAGGAAATCGACTGGGCCCAGCTGAACCATCGCCAAATAGAACCGCCTTTCAGACCC
AGAATCAAATCCCGAGAAGATGTCAGTAATTTTGACCCTGACTTCATAAAGGAAGAGCCA
GTTTTAACTCCAATTGATGAGGGACATCTTCCAATGATTAACCAGGATGAGTTTAGAAAC
TTTTCCTATGTGTCTCCAGAATTGCAACCATAGCCTTATGGGGAGTGAGAGAGAGGGCAC
GAGAACCCAAAGGGAATAGAGATTCTCCAGGAATTTCCTCTATGGGACCTTCCCAGCATC
AGCCTTAGAACAAGAACCTTACCTTCAAGGAGCAAGTGAAGAACTCTGTGAAGGATGGAA
CTTTCAGA
>OriGene 5' read for NM_006255 unedited
NGGGGTCAAATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCCGGT
TCTCCCGCTGCGAACAGCGCGGCCCCCCGGGGCCGGGGCAGCGGCGCCGGCATGTCGTCT
GGCACCATGAAGTTCAATGGCTATTTGAGGGTCCGCATCGGTGAGGCAGTGGGGCTGCAG
CCCACCCGCTGGTCCCTGCGCCACTCGCTCTTCAAGAAGGGCCACCAGCTGCTGGACCCC
TATCTGACGGTGAGCGTGGACCAGGTGCGCGTGGGCCAGACCAGCACCAAGCAGAAGACC
AACAAACCCACGTACAACGAGGAGTTTTGCGCTAACGTCACCGACGGCGGCCACCTCGAG
TTGGCCGTCTTCCACGAGACGCCCCTGGGCTACGACCACTTCGTGGCCAACTGCACCCTG
CAGTTCCAGGAGCTGCTGCGCACGACCGGCGCCTCGGACACCTTCGAGGGTTGGGTGGAT
CTCGAGCCAGAGGGGAAAGTATTTGTGGTAATAACCCTTACCGGGAGTTTCACTGAAGCT
ACTCTCCAGAGAGACCGGATCTTCAAACATTTTACCAGGAAGCGCCAAAGGGCTATGCGA
AGGCGAGTCCACCAGATCAATGGACACAAGTTCATGGCCACGTATCTGAGGCAGCCCACC
TACTGCTCTCACTGCAGGGAGTTTATCTGGGGAGTGTTTGGGAAACAGGGNTATCAGTGC
CAAGTGTGCACCTGTGTCGTCCATAAACGCTGCCATCATCTAATTGTTACAGCCTGTACT
TGCCAAAACAATATTAACAAAGTGGATTCAAAGATTGCAGAACAGAGTTCCGGGATCAAC
ATCCCACACAAGTTCAGCATTCACACTTACCAAGTGCCAACATTCTGCGATCACTGTGGC
TCACTGCTT
>OriGene 3' read for NM_006255 unedited
TTGTACGCGGCCGCATTCTANGATCGGTTTTTTTTTTTTTTTTTTCCTTTTGCTACAAAA
TGCATTTATTTCCAGTCAGACAAAATTCTTGAACTGTATATTCGGGTACATATTATAAGG
TCATCTTTGATATATGCCCTGTTTACAAGATCCATTTCTAATTATTAAAGTGAATATCAA
ACACACATCTTTGCATCTTAAAACATAAATAAACTGCTAATGTTTATAAGCCACAAATCT
GGTCACTGACATGAGTGGCCTGCAATTCTTCAGTGATGAGCACACGGCGATACAGACAAG
ATCAGATTACCCAGGTAGGCAGGCAGCAGGCACCTGTAATTCACCCGCTGACTGTGCTGT
TGTGGCAGTGACCCTTCTTGTGAAAAAAGAGTTATGTGCAGACAAAAGTGTGACATATGC
AACGTGGAGAGGCATTTCACAGAATGCAAACACCATTCAGGTAAATTAGTAGACTCCAGA
ATGAATGAAAGCTTCATGAATGCAAACCTGTTGTTTACAATACTGGGCATTGTGGCACCT
TTTCACTGGACAGTTTAATAAATATCTATTAAAGAAAGTAAGGATAAGAGAAGAAGCTGA
AGCAGTCGTCTTGGTGCACAACATATTTTCAAGATTGGGCTTTAGGTTGTGAGTTTCAGA
GATACCATTGTTTGTTTCTTCAGAGTAATCATTCTATTTGGAAAGCCCTTCATTTCCNNT
AAAAGACCTTTAAATTCTCATTAGCATTAAGAGACTTCTTGTAGACTGCCAGGAATGGGG
TTTCATTGGCTTTAAGAGAGCACGCACAGTTGACAGACGGTCTGGTTGTCTCTTGAATAA
GAGGCTGATGGCTCAAATTTCTCAGATCACTTTATCTCCAACTTTCCCTCCTTTGGCTCT
GGTAACATCCAAGCCAAGAGACAGAGCTCCTAGTGGGACGGTTTCTTCATAAATN
Restriction Sites NotI-NotI     
ACCN NM_006255
Insert Size 3500 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006255.3, NP_006246.2
RefSeq Size 3522 bp
RefSeq ORF 2052 bp
Locus ID 5583
Cytogenetics 14q23.1
Domains C2, pkinase, S_TK_X, TyrKc, DAG_PE-bind, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Tight junction, Vascular smooth muscle contraction
Gene Summary 'Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. Mutations in this gene are associated with susceptibility to cerebral infarction. [provided by RefSeq, Sep 2015]'

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