PKC eta (PRKCH) (NM_006255) Human Untagged Clone

CAT#: SC116250

PRKCH (untagged)-Human protein kinase C, eta (PRKCH)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRKCH
• Synonyms: nPKC-eta; PKC-L; PKCL; PRKCL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_006255 edited
  GCGGCCGCGAATTCGGCACGAGGCCCGGTTCTCCCGCTGCGAAGCAGCGCGGCCCCCCGG
  GGCCGGGGCAGCGGCGCCGGCATGTCGTCTGGCACCATGAAGTTCAATGGCTATTTGAGG
  GTCCGCATCGGTGAGGCAGTGGGGCTGCAGCCCACCCGCTGGTCCCTGCGCCACTCGCTC
  TTCAAGAAGGGCCACCAGCTGCTGGACCCCTATCTGACGGTGAGCGTGGACCAGGTGCGC
  GTGGGCCAGACCAGCACCAAGCAGAAGACCAACAAACCCACGTACAACGAGGAGTTTTGC
  GCTAACGTCACCGACGGCGGCCACCTCGAGTTGGCCGTCTTCCACGAGACGCCCCTGGGC
  TACGACCACTTCGTGGCCAACTGCACCCTGCAGTTCCAGGAGCTGCTGCGCACGACCGGC
  GCCTCGGACACCTTCGAGGGTTGGGTGGATCTCGAGCCAGAGGGGAAAGTATTTGTGGTA
  ATAACCCTTACCGGGAGTTTCACTGAAGCTACTCTCCAGAGAGACCGGATCTTCAAACAT
  TTTACCAGGAAGCGCCAAAGGGCTATGCGAAGGCGAGTCCACCAGATCAATGGACACAAG
  TTCATGGCCACGTATCTGAGGCAGCCCACCTACTGCTCTCACTGCAGGGAGTTTATCTGG
  GGAGTGTTTGGGAAACAGGGTTATCAGTGCCAAGTGTGCACCTGTGTCGTCCATAAACGC
  TGCCATCATCTAATTGTTACAGCCTGTACTTGCCAAAACAATATTAACAAAGTGGATTCA
  AAGATTGCAGAACAGAGGTTCGGGATCAACATCCCACACAAGTTCAGCATCCACAACTAC
  AAAGTGCCAACATTCTGCGATCACTGTGGCTCACTGCTCTGGGGAATAATGCGACAAGGA
  CTTCAGTGTAAAATATGTAAAATGAATGTGCATATTCGATGTCAAGCGAACGTGGCCCCT
  AACTGTGGGGTAAATGCGGTGGAACTTGCCAAGACCCTGGCAGGGATGGGTCTCCAACCC
  GGAAATATTTCTCCAACCTCGAAACTCGTTTCCAGATCGACCCTAAGACGACAGGGAAAG
  GAGAGCAGCAAAGAAGGAAATGGGATTGGGGTTAATTCTTCCAACCGACTTGGTATCGAC
  AACTTTGAGTTCATCCGAGTGTTGGGGAAGGGGAGTTTTGGGAAGGTGATGCTTGCAAGA
  GTAAAAGAAACAGGAGACCTCTATGCTGTGAAGGTGCTGAAGAAGGACGTGATTCTGCAG
  GATGATGATGTGGAATGCACCATGACCGAGAAAAGGATCCTGTCTCTGGCCCGCAATCAC
  CCCTTCCTCACTCAGTTGTTCTGCTGCTTTCAGACCCCCGATCGTCTGTTTTTTGTGATG
  GAGTTTGTGAATGGGGGTGACTTGATGTTCCACATTCAGAAGTCTCGTCGTTTTGATGAA
  GCACGAGCTCGCTTCTATGCTGCAGAAATCATTTCGGCTCTCATGTTCCTCCATGATAAA
  GGAATCATCTATAGAGATCTGAAACTGGACAATGTCCTGTTGGACCACGAGGGTCACTGT
  AAACTGGCAGACTTCGGAATGTGCAAGGAGGGGATTTGCAATGGTGTCACCACGGCCACA
  TTCTGTGGCACGCCAGACTATATCGCTCCAGAGATCCTCCAGGAAATGCTGTACGGGCCT
  GCAGTAGACTGGTGGGCAATGGGCGTGTTGCTCTATGAGATGCTCTGTGGTCACGCGCCT
  TTTGAGGCAGAGAATGAAGATGACCTCTTTGAGGCCATACTGAATGATGAGGTGGTCTAC
  CCTACCTGGCTCCATGAAGATGCCACAGGGATCCTAAAATCTTTCATGACCAAGAACCCC
  ACCATGCGCTTGGGCAGCCTGACTCAGGGAGGCGAGCACGCCATCTTGAGACATCCTTTT
  TTTAAGGAAATCGACTGGGCCCAGCTGAACCATCGCCAAATAGAACCGCCTTTCAGACCC
  AGAATCAAATCCCGAGAAGATGTCAGTAATTTTGACCCTGACTTCATAAAGGAAGAGCCA
  GTTTTAACTCCAATTGATGAGGGACATCTTCCAATGATTAACCAGGATGAGTTTAGAAAC
  TTTTCCTATGTGTCTCCAGAATTGCAACCATAGCCTTATGGGGAGTGAGAGAGAGGGCAC
  GAGAACCCAAAGGGAATAGAGATTCTCCAGGAATTTCCTCTATGGGACCTTCCCAGCATC
  AGCCTTAGAACAAGAACCTTACCTTCAAGGAGCAAGTGAAGAACTCTGTGAAGGATGGAA
  CTTTCAGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006255 unedited
  NGGGGTCAAATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCCGGT
  TCTCCCGCTGCGAACAGCGCGGCCCCCCGGGGCCGGGGCAGCGGCGCCGGCATGTCGTCT
  GGCACCATGAAGTTCAATGGCTATTTGAGGGTCCGCATCGGTGAGGCAGTGGGGCTGCAG
  CCCACCCGCTGGTCCCTGCGCCACTCGCTCTTCAAGAAGGGCCACCAGCTGCTGGACCCC
  TATCTGACGGTGAGCGTGGACCAGGTGCGCGTGGGCCAGACCAGCACCAAGCAGAAGACC
  AACAAACCCACGTACAACGAGGAGTTTTGCGCTAACGTCACCGACGGCGGCCACCTCGAG
  TTGGCCGTCTTCCACGAGACGCCCCTGGGCTACGACCACTTCGTGGCCAACTGCACCCTG
  CAGTTCCAGGAGCTGCTGCGCACGACCGGCGCCTCGGACACCTTCGAGGGTTGGGTGGAT
  CTCGAGCCAGAGGGGAAAGTATTTGTGGTAATAACCCTTACCGGGAGTTTCACTGAAGCT
  ACTCTCCAGAGAGACCGGATCTTCAAACATTTTACCAGGAAGCGCCAAAGGGCTATGCGA
  AGGCGAGTCCACCAGATCAATGGACACAAGTTCATGGCCACGTATCTGAGGCAGCCCACC
  TACTGCTCTCACTGCAGGGAGTTTATCTGGGGAGTGTTTGGGAAACAGGGNTATCAGTGC
  CAAGTGTGCACCTGTGTCGTCCATAAACGCTGCCATCATCTAATTGTTACAGCCTGTACT
  TGCCAAAACAATATTAACAAAGTGGATTCAAAGATTGCAGAACAGAGTTCCGGGATCAAC
  ATCCCACACAAGTTCAGCATTCACACTTACCAAGTGCCAACATTCTGCGATCACTGTGGC
  TCACTGCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006255 unedited
  TTGTACGCGGCCGCATTCTANGATCGGTTTTTTTTTTTTTTTTTTCCTTTTGCTACAAAA
  TGCATTTATTTCCAGTCAGACAAAATTCTTGAACTGTATATTCGGGTACATATTATAAGG
  TCATCTTTGATATATGCCCTGTTTACAAGATCCATTTCTAATTATTAAAGTGAATATCAA
  ACACACATCTTTGCATCTTAAAACATAAATAAACTGCTAATGTTTATAAGCCACAAATCT
  GGTCACTGACATGAGTGGCCTGCAATTCTTCAGTGATGAGCACACGGCGATACAGACAAG
  ATCAGATTACCCAGGTAGGCAGGCAGCAGGCACCTGTAATTCACCCGCTGACTGTGCTGT
  TGTGGCAGTGACCCTTCTTGTGAAAAAAGAGTTATGTGCAGACAAAAGTGTGACATATGC
  AACGTGGAGAGGCATTTCACAGAATGCAAACACCATTCAGGTAAATTAGTAGACTCCAGA
  ATGAATGAAAGCTTCATGAATGCAAACCTGTTGTTTACAATACTGGGCATTGTGGCACCT
  TTTCACTGGACAGTTTAATAAATATCTATTAAAGAAAGTAAGGATAAGAGAAGAAGCTGA
  AGCAGTCGTCTTGGTGCACAACATATTTTCAAGATTGGGCTTTAGGTTGTGAGTTTCAGA
  GATACCATTGTTTGTTTCTTCAGAGTAATCATTCTATTTGGAAAGCCCTTCATTTCCNNT
  AAAAGACCTTTAAATTCTCATTAGCATTAAGAGACTTCTTGTAGACTGCCAGGAATGGGG
  TTTCATTGGCTTTAAGAGAGCACGCACAGTTGACAGACGGTCTGGTTGTCTCTTGAATAA
  GAGGCTGATGGCTCAAATTTCTCAGATCACTTTATCTCCAACTTTCCCTCCTTTGGCTCT
  GGTAACATCCAAGCCAAGAGACAGAGCTCCTAGTGGGACGGTTTCTTCATAAATN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006255
• Insert Size: 3500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006255.3, NP_006246.2
• RefSeq Size: 3522 bp
• RefSeq ORF: 2052 bp
• Locus ID: 5583
• Cytogenetics: 14q23.1
• Domains: C2, pkinase, S_TK_X, TyrKc, DAG_PE-bind, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Tight junction, Vascular smooth muscle contraction
• Gene Summary: 'Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. Mutations in this gene are associated with susceptibility to cerebral infarction. [provided by RefSeq, Sep 2015]'