cGKI (PRKG1) (NM_006258) Human Untagged Clone

CAT#: SC116252

PRKG1 (untagged)-Human protein kinase, cGMP-dependent, type I (PRKG1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRKG1
• Synonyms: AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha; cGKI-BETA; PKG; PKG1; PRKG1B; PRKGR1B
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_006258 edited
  CCGGAGGAGCATGGGCACCTTGCGGGATTTACAGTACGCGCTCCAGGAGAAGATCGAGGA
  GCTGAGGCAGCGGGATGCTCTCATCGACGAGCTGGAGCTGGAGTTGGATCAGAAGGACGA
  ACTGATCCAGAAGCTGCAGAACGAGCTGGACAAGTACCGCTCGGTGATCCGACCAGCCAC
  CCAGCAGGCGCAGAAGCAGAGCGCGAGCACCTTGCAGGGCGAGCCGCGCACCAAGCGGCA
  GGCGATCTCCGCCGAGCCCACCGCCTTCGACATCCAGGATCTCAGCCATGTGACCCTGCC
  CTTCTACCCCAAGAGCCCACAGTCCAAGGATCTTATAAAGGAAGCTATCCTTGACAATGA
  CTTTATGAAGAACTTGGAGCTGTCGCAGATCCAGGAGATTGTGGATTGTATGTACCCGGT
  GGAGTATGGCAAGGACAGTTGCATCATCAAAGAAGGAGACGTGGGGTCACTGGTGTATGC
  CATGGAAGATGGTAAGGTTGAAGTTACAAAAGAAGGTGTGAAGTTGTGTACCATGGGTCC
  AGGAAAAGTGTTTGGGGAATTGGCTATTCTTTACAACTGTACCCGGACAGCGACCGTCAA
  GACTCTTGTAAATGTAAAACTCTGGGCCATTGATCGACAATGTTTTCAAACAATAATGAT
  GAGGACAGGACTCATCAAGCATACCGAGTATATGGAATTTTTAAAAAGCGTTCCAACATT
  CCAGAGCCTTCCTGAAGAGATCCTCAGCAAGCTTGCTGATGTCCTTGAAGAGACCCACTA
  TGAAAATGGAGAATATATTATCAGGCAAGGTGCAAGAGGGGACACCTTCTTTATCATCAG
  CAAAGGAACGGTAAATGTCACTCGTGAAGACTCACCGAGTGAAGACCCAGTCTTTCTTAG
  AACTTTAGGAAAAGGAGACTGGTTTGGAGAGAAAGCCTTGCAGGGGGAAGATGTGAGAAC
  AGCAAACGTAATTGCTGCAGAAGCTGTAACCTGCCTTGTGATTGACAGAGACTCTTTTAA
  ACATTTGATTGGAGGGCTGGATGATGTTTCTAATAAAGCATATGAAGATGCAGAAGCTAA
  AGCAAAATATGAAGCTGAAGCGGCTTTCTTCGCCAACCTGAAGCTGTCTGATTTCAACAT
  CATTGATACCCTTGGAGTTGGAGGTTTCGGACGAGTAGAACTGGTCCAGTTGAAAAGTGA
  AGAATCCAAAACGTTTGCAATGAAGATTCTCAAGAAACGTCACATTGTGGACACAAGACA
  GCAGGAGCACATCCGCTCAGAGAAGCAGATCATGCAGGGGGCTCATTCCGATTTCATAGT
  GAGACTGTACAGAACATTTAAGGACAGCAAATATTTGTATATGTTGATGGAAGCTTGTCT
  AGGTGGAGAGCTCTGGACCATTCTCAGGGATAGAGGTTCGTTTGAAGATTCTACAACCAG
  ATTTTACACAGCATGTGTGGTAGAAGCTTTTGCCTATCTGCATTCCAAAGGAATCATTTA
  CAGGGACCTCAAGCCAGAAAATCTCATCCTAGATCACCGAGGTTATGCCAAACTGGTTGA
  TTTTGGCTTTGCAAAGAAAATAGGATTTGGAAAGAAAACATGGACTTTTTGTGGGACTCC
  AGAGTATGTAGCCCCAGAGATCATCCTGAACAAAGGCCATGACATTTCAGCCGACTACTG
  GTCACTGGGAATCCTAATGTATGAACTCCTGACTGGCAGCCCACCTTTCTCAGGCCCAGA
  TCCTATGAAAACCTATAACATCATATTGAGGGGGATTGACATGATAGAATTTCCAAAGAA
  GATTGCCAAAAATGCTGCTAATTTAATTAAAAAACTATGCAGGGACAATCCATCAGAAAG
  ATTAGGGAATTTGAAAAATGGAGTAAAAGACATTCAAAAGCACAAATGGTTTGAGGGCTT
  TAACTGGGAAGGCTTAAGAAAAGGTACCTTGACACCTCCTATAATACCAAGTGTTGCATC
  ACCCACAGACACAAGTAATTTTGACAGTTTCCCTGAGGACAACGATGAACCACCACCTGA
  TGACAACTCAGGATGGGATATAGACTTCTAATGTATTTCTCTTACCTGCTTCTGCCTTGC
  TGAAGACAGCTTTTTCTGAGACACAGCTGCCAGCAAACCTGAGGGAAAGAGAGAAGATTA
  GTGCTCGGGGTCACCATGATGCCTTTGATCGATGCTGCTCCAGTAACTACAGTGGCATTA
  GGACTTACCGCTTAGATGACAATAGTGCTCTTTACATGTTTTCTGTTTGAACCTAAAATA
  GCAGTTGACATGGTGGTCCTGAAGCAAAGCCTTTCACCAGTAAAAGATGTTTTCTATTGT
  TGCAATGACCTTGCTTTGCTCTGATTATAATTTGAAAGACTGTAGGAAACACTTCAATGT
  AGTATAAGAGTCTGTACCTTGCTGGAATATTCAAGAAGATGAAAGAATAATATATTGGGT
  ACAATAGATTACTATGGTACAGAAACTGGGCTATTCCCTTTCTTCAAGTGAAGGCTGTGG
  GATCTATTACTGCAGGCCGGTGTATATACCATACAAAAGAGGACCACACATCTGTTGGTC
  ACAGAGTTCATGTCACACCAGTGCTAGAAGTTTCATGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006258 unedited
  AGTTTCTTTTGTATACACCCTTAGGCGGCCGGATTCGCACGAGACGGGTGGGGAGGAAGC
  CTCAATACGCGGAGCAGCGGCAGGAAGGAGCCCCCGGCAGCCCGGAGGAGCATGGGCACC
  TTGCGGGATTTACAGTACGCGCTCCATGAGAAGATCGAGGAGCTGAGGCAGCGGGATGCT
  CTCATCGACGAGCTGGAGCTGGAGTTGGATCATAAGGACGAACTGATCCAGAAGCTGCAG
  AACGAGCTGGACAAGTACCGCTCGGTGATCCGACCAGCCACCCATCAGGCGCATAATCAG
  ATCGCGAGCACCTTGCATGGCGAGCCGCGCACCAAGCGGCAGGCGATCTCCGCCGAGCCC
  ACCGCCTTCGACATCCAGGATCTCAGCCATGTGACCCTGCCCTTCTACCCCAAGAGCCCA
  CAGTCCAAGGATCTTATAAAGGAAGCTATCCTTGACAATGACTTTATGAAGAACTTGGAG
  CTGTCGCATATCCAGGAGATTGTGGATTGTATGTACCCGGTGGAGTATGGCAAGGACAGG
  TGCATCATCAAAGAATGAGACGTGGGGTCACTGGTGTATGCCATGGAAGATGGTAAGGTC
  TGAAGTTACAAAAGAAGGTGTGAAGTTGTGTACCATGGGTCCAGGATAAGTGTTTGGGGG
  AATTGGCTATTCTTTACAACTGTACCCGGACAGCGACCGTCAAGACTCTTGTGAATGGAA
  AACTCTGGGCCATTGATCGACAATGGTTTCAAACNATAATGATGAGGACAGGACTCATCA
  AGCATACCGAGTATATGGAATTTTTAAAAAGCGTGCCACATTCCAGAGCCTTCCTGNAGA
  GATCCTCANCAAGCGTGCTGATGTCCTTGAAAAGACCACTATGGAAATGGGGATATATTA
  TCAGGCCAGGGGGCAGAAGGGACA
  
• Restriction Sites: Please inquire
• ACCN: NM_006258
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006258.1, NP_006249.1
• RefSeq Size: 3740 bp
• RefSeq ORF: 2061 bp
• Locus ID: 5592
• Cytogenetics: 10q11.23-q21.1
• Domains: cNMP, pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Gap junction, Long-term depression, Olfactory transduction, Vascular smooth muscle contraction
• Gene Summary: 'Mammals have three different isoforms of cyclic GMP-dependent protein kinase (Ialpha, Ibeta, and II). These PRKG isoforms act as key mediators of the nitric oxide/cGMP signaling pathway and are important components of many signal transduction processes in diverse cell types. This PRKG1 gene on human chromosome 10 encodes the soluble Ialpha and Ibeta isoforms of PRKG by alternative transcript splicing. A separate gene on human chromosome 4, PRKG2, encodes the membrane-bound PRKG isoform II. The PRKG1 proteins play a central role in regulating cardiovascular and neuronal functions in addition to relaxing smooth muscle tone, preventing platelet aggregation, and modulating cell growth. This gene is most strongly expressed in all types of smooth muscle, platelets, cerebellar Purkinje cells, hippocampal neurons, and the lateral amygdala. Isoforms Ialpha and Ibeta have identical cGMP-binding and catalytic domains but differ in their leucine/isoleucine zipper and autoinhibitory sequences and therefore differ in their dimerization substrates and kinase enzyme activity. [provided by RefSeq, Sep 2011]'
  Transcript Variant: This variant (2) uses an alternate exon at its 5' end which result in the use of a distinct translation initiation site, compared to variant 1. This difference results in an isoform (2; also known as beta or 1beta) with a longer and distinct N-terminus, compared to isoform 1.