Hsp40 (DNAJB1) (NM_006145) Human Untagged Clone

CAT#: SC116273

DNAJB1 (untagged)-Human DnaJ (Hsp40) homolog, subfamily B, member 1 (DNAJB1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DNAJB1
• Synonyms: Hdj1; Hsp40; HSPF1; RSPH16B; Sis1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116273 sequence for NM_006145 edited (data generated by NextGen Sequencing)
  ATGGGTAAAGACTACTACCAGACGTTGGGCCTGGCCCGCGGCGCGTCGGACGAGGAGATC
  AAGCGGGCCTACCGCCGCCAGGCGCTGCGCTACCACCCGGACAAGAACAAGGAGCCCGGC
  GCCGAGGAGAAGTTCAAGGAGATCGCTGAGGCCTACGACGTGCTCAGCGACCCGCGCAAG
  CGCGAGATCTTCGACCGCTACGGGGAGGAAGGCCTAAAGGGGAGTGGCCCCAGTGGCGGT
  AGCGGCGGTGGTGCCAATGGTACCTCTTTCAGCTACACATTCCATGGAGACCCTCATGCC
  ATGTTTGCTGAGTTCTTCGGTGGCAGAAATCCCTTTGACACCTTTTTTGGGCAGCGGAAC
  GGGGAGGAAGGCATGGACATTGATGACCCATTCTCTGGCTTCCCTATGGGCATGGGTGGC
  TTCACCAACGTGAACTTTGGCCGCTCCCGCTCTGCCCAAGAGCCCGCCCGAAAGAAGCAA
  GATCCCCCAGTCACCCACGACCTTCGAGTCTCCCTTGAAGAGATCTACAGCGGCTGTACC
  AAGAAGATGAAAATCTCCCACAAGCGGCTAAACCCCGACGGAAAGAGCATTCGAAACGAA
  GACAAAATATTGACCATCGAAGTGAAGAAGGGGTGGAAAGAAGGAACCAAAATCACTTTC
  CCCAAGGAAGGAGACCAGACCTCCAACAACATTCCAGCTGATATCGTCTTTGTTTTAAAG
  GACAAGCCCCACAATATCTTTAAGAGAGATGGCTCTGATGTCATTTATCCTGCCAGGATC
  AGCCTCCGGGAGGCTCTGTGTGGCTGCACAGTGAACGTCCCCACTCTGGACGGCAGGACG
  ATACCCGTCGTATTCAAAGATGTTATCAGGCCTGGCATGCGGCGAAAAGTTCCTGGAGAA
  GGCCTCCCCCTCCCCAAAACACCCGAGAAACGTGGGGACCTCATTATTGAGTTTGAAGTG
  ATCTTCCCCGAAAGGATTCCCCAGACATCAAGAACCGTACTTGAGCAGGTTCTTCCAATA
  TAG
  
  Clone variation with respect to NM_006145.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006145 unedited
  AGACAGAATTGTATACGACTTACTATAGGCGGCCGCGAATTCGCACGAGGTAGAGTCCGG
  GACTGGTCGGCGGCGGAGCCGGGGGACGGCGACAGCGGGTCGGCGGGCCGCAGGAGGGGG
  TCATGGGTAAAGACTACTACCAGACGTTGGGCCTGGCCCGCGGCGCGTCGGACGAGGAGA
  TCAAGCGGGCCTACCGCCGCCAGGCGCTGCGCTACCACCCGGACAAGAACAAGGAGCCCG
  GCGCCGAGGAGAAGTTCAAGGAGATCGCTGAGGCCTACGACGTGCTCAGCGACCCGCGCA
  AGCGCGAGATCTTCGACCGCTACGGGGAGGAAGGCCTAAAGGGGAGTGGCCCCAGTGGCG
  GTAGCGGCGGTGGTGCCAATGGTACCTCTTTCAGCTACACATTCCATGGAGACCCTCATG
  CCATGTTTGCTGAGTTCTTCGGTGGCAGAAATCCCTTTGACACCTTTTTTGGGCAGCGGA
  ACGGGGAGGAAGGCATGGACATTGATGACCCATTCTCTGGCTTCCCTATGGGCATGGGTG
  GCTTCACCAACGTGAACTTTGGCCGCTCCCGCTCTGCCCAAGAGCCCGCCCGAAAGAAGC
  AAGATCCCCCAGTCACCCACGACCTTCGAGTCTCCCTTGAAGAGATCTACAGCGGCTGTA
  CCAAGAAGATGAAAATCTCCCACAAGCGGCTAAACCCCGACGGAAAGAGCATTCGAAACG
  AAGACAAAATATTGACCATCGAAGTGAAGAAGGGGTGGAAAGAANGAACCAAAATCACTT
  TCCCCAAGGAAGGAGACCAGACCTCCAACAACATTCCAGCTGATATCGTCTTTGTTTTAA
  AGGACAAGCCCCACATATCTTTAGAGAGATGGCTCTGATGTCTTTATCCTGCCAGGATCA
  GCCTCCGGGAGCTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006145 unedited
  GAGTCGAGTTTTTTTTTTTTTTTTTTGAGGTTTAGCATCAGTCTTTAATGCTGTCGCACT
  TCATTGACTAGTCACACACTTTGGTCTCATGTTGGCAGTGGCAACTTACAATGAGTCTAA
  AGGTCTGAGCACTGGACTGGCCTCCAGGGAACAGACATTTGTTCCAACTCCCCTTCCCTC
  CCCAAGATTTCTTCAGAATTCCATTATGGCCCTATACAGAGAGCACTGGCTGCCGGGGAC
  AGGTTTTGAGTGTACACCAACTATACATGGAATTATAAAAACATATTTACAGACGTTCCA
  CAGTGCTCCTGTAATCAGAAGCAAAGACCCTTTTATCAAAAGGGATTGTATCTAGGGCTG
  TGCAAAATTCAAAAGGATCAGATCCCTTTGAAAGAGTCCATAGTCCATGAAACAAAAAAT
  ACCCTGGCCACTGGTAAGCCCCACGTGTGCCAAGATTGCCTTACAGAAATGTAAAGAGTG
  TGACCCACAAAGTGAGGACATTCAGCTTTACTGGAGCCAGAGGTCAGGAAAGGCCCCTTG
  CTGAGGCCACGCTCCCAGACCATCAAGGGAAGAGGTGGTTGCACTTGCTGGGATCACTCA
  CTTGAAGCGTGCTCCCTTGGTCCTGCTGGAACAACAGGGATTGTTGGTGACCCCACCCCG
  CAGGTCTTCCGGAATCTTTAAGGTTTACACTTGGCACGGCACAAAGTAAAAACACCTTCC
  CAGAGATCAACCATACTTGGCGAACAATTCCGAACAACGCCCATCCTCGTCCCCCCCTTT
  ACAAGGAGGGGGGGGCAATGGGGTCTGGCAATCCCCCTCGCCCGCGTGCCTTTTTTATCT
  TCCCTATATCCAACGACACCCCGCGCTCATGCCCCCCGCGGGCCGCCCATCCTACATCAT
  GGCAGGTACTTGGTGTGGGTTTGTGTAACTTTCGGGAGGCGCGTAACACCATGGGGGCCA
  AAGCGATTGCCCTTTCGGCTAGCCATCCACGTTAGCATTAAATTATCACAACCCCCGTTA
  TCTGCGCCCACCAACAAGGCATTCGCCGTTCAATACATTTCCCCCACCCCTCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006145
• Insert Size: 2290 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006145.1, NP_006136.1
• RefSeq Size: 2233 bp
• RefSeq ORF: 1023 bp
• Locus ID: 3337
• Cytogenetics: 19p13.12
• Domains: DnaJ, DnaJ_C
• Gene Summary: 'This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1).