Neurofilament (NEFL) (NM_006158) Human Untagged Clone

CAT#: SC116280

NEFL (untagged)-Human neurofilament, light polypeptide (NEFL)


  "NM_006158" in other vectors (6)

Reconstitution Protocol

USD 760.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol NEFL
Synonyms CMT1F; CMT2E; CMTDIG; NF-L; NF68; NFL; PPP1R110
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_006158, the custom clone sequence may differ by one or more nucleotides


ATGAGTTCCTTCAGCTACGAGCCGTACTACTCGACCTCCTACAAGCGGCGCTACGTGGAGACGCCCCGGG
TGCACATCTCCAGCGTGCGCAGCGGCTACAGCACCGCACGCTCAGCTTACTCCAGCTACTCGGCGCCGGT
GTCTTCCTCGCTGTCCGTGCGCCGCAGCTACTCCTCCAGCTCTGGATCGTTGATGCCCAGTCTGGAGAAC
CTCGACCTGAGCCAGGTAGCCGCCATCAGCAACGACCTCAAGTCCATCCGCACGCAGGAGAAGGCGCAGC
TCCAGGACCTCAATGACCGCTTCGCCAGCTTCATCGAGCGCGTGCACGAGCTGGAGCAGCAGAACAAGGT
CCTGGAAGCCGAGCTGCTGGTGCTGCGCCAGAAGCACTCCGAGCCATCCCGCTTCCGGGCGCTGTACGAG
CAGGAGATCCGCGACCTGCGCCTGGCGGCGGAAGATGCCACCAACGAGAAGCAGGCGCTCCAGGGCGAGC
GCGAAGGGCTGGAGGAGACCCTGCGCAACCTGCAGGCGCGCTATGAAGAGGAGGTGCTGAGCCGCGAGGA
CGCCGAGGGCCGGCTGATGGAAGCGCGCAAAGGCGCCGACGAGGCGGCGCTCGCTCGCGCCGAGCTCGAG
AAGCGCATCGACAGCTTGATGGACGAAATCTCTTTTCTGAAGAAAGTGCACGAAGAGGAGATCGCCGAAC
TGCAGGCGCAGATCCAGTACGCGCAGATCTCCGTGGAGATGGACGTGACCAAGCCCGACCTTTCCGCCGC
GCTCAAGGACATCCGCGCGCAGTACGAGAAGCTGGCCGCCAAGAACATGCAGAACGCTGAGGAATGGTTC
AAGAGCCGCTTCACCGTGCTGACCGAGAGCGCCGCCAAGAACACCGACGCCGTGCGCGCCGCCAAGGACG
AGGTGTCCGAGAGCCGTCGTCTGCTCAAGGCCAAGACCCTGGAAATCGAAGCATGCCGGGGCATGAATGA
AGCGCTGGAGAAGCAGCTGCAGGAGCTGGAGGACAAGCAGAACGCCGACATCAGCGCTATGCAGGACACG
ATCAACAAATTAGAAAATGAATTGAGGACCACAAAGAGTGAAATGGCACGATACCTAAAAGAATACCAAG
ACCTCCTCAACGTGAAGATGGCTTTGGATATTGAGATTGCAGCTTACAGGAAACTCTTGGAAGGCGAGGA
GACCCGACTCAGTTTCACCAGCGTGGGAAGCATAACCAGTGGCTACTCCCAGAGCTCCCAGGTCTTTGGC
CGATCTGCCTACGGCGGTTTACAGACCAGCTCCTATCTGATGTCCACCCGCTCCTTCCCGTCCTACTACA
CCAGCCATGTCCAAGAGGAGCAGATCGAAGTGGAGGAAACCATTGAGGCTGCCAAGGCTGAGGAAGCCAA
GGATGAGCCCCCCTCTGAAGGAGAAGCCGAGGAGGAGGAGAAGGACAAGGAAGAGGCCGAGGAAGAGGAG
GCAGCTGAAGAGGAAGAAGCTGCCAAGGAAGAGTCTGAAGAAGCAAAAGAAGAAGAAGAAGGAGGTGAAG
GTGAAGAAGGAGAGGAAACCAAAGAAGCTGAAGAGGAGGAGAAGAAAGTTGAAGGTGCTGGGGAGGAACA
AGCAGCTAAGAAGAAAGATTGA


>OriGene 5' read for NM_006158 unedited
NGGTGCNAATTTGTATACGACCACTATAGGCGGCCGCGNAATCGGCACGAGGCATCCTCC
CCCTTCCCTCTCTCCCCTGTCCTCTCTCTCCGGGCTCCCACCGCCGCCGCGGGCCGGGGA
GCCACCGGCCGCCACCATGAGTTCCTTCAGCTACGAGCCGTACTACTCGACCTCCTACAA
GCGGCGCTACGTGGAGACGCCCCGGGTGCACATCTCCAGCGTGCGCAGCGGCTACAGCAC
CGCACGCTCAGCTTACTCCAGCTACTCGGCGCCGGTGTCTTCCTCGCTGTCCGTGCGCCG
CAGCTACTCCTCCAGCTCTGGATCGTTGATGCCCAGTCTGGAGAACCTCGACCTGAGCCA
GGTAGCCGCCATCAGCAACGACCTCAAGTCCATCCGCACGCAGGAGAAGGCGCAGCTCCA
GGACCTCAATGACCGCTTCGCCAGCTTCATCGAGCGCGTGCACGAGCTGGAGCAGCAGAA
CAAGGTCCTGGAAGCCGAGCTGCTGGTGCTGCGCCAGAAGCACTCCGAGCCATCCCGCTT
CCGGGCGCTGTACGAGCAGGAGATCCGCGACCTGCGCCTGGCGGCGGAAGATGCCACCAA
CGAGAAGCACGCGCTCCAGGGCGAGCGCGAAGGGCTGGAGGAGACCCTGCGCAACCTGCA
GGCGCGCTATGAAGAGGAGGTGCTGAGCCCCGAGGACGCCGAAGGCCGGCTGATGGAAGC
CCGCAAAGGCGCCGACGATGCGGCGCTCGCTCGCGCCGAGCTCCGAGAGCGCATGGACAG
CTTGATGGACCAAATCTCTTTTCTGAAGACAGTGCCCGAAGAAGAGATCGCCCGACTGCC
AGCGCAGATCCCATACCCCCACACCCCGGGCAGAGGACGTTGACAAGCCCGACCTACCGC
CG
>OriGene 3' read for NM_006158 unedited
NTTTTAGCTCTGNNACCGCGGCCGCATNCTAGNGATCGGTTTTTTTTTTTTTTTTTTACA
TCATTCGGTATAACTTTATTTACTATTTATTGCACATAACTCAGGGTCTTTAATTGCTAA
CCACCGAAGGTTCAAAGGACTATTTTTCCATGAAGTTTAACATTAACATTAGTGAATTCA
TTTACTCATGTGGTGTTTTTTATTGTAAACATCTGAATGATTCACATTGCCGTAGATCCT
GAACTCATAAGCGTGGTCCATGCACAGGCTGGCAGCAAGCCAGAAAGCCACTCTGCAAGC
AAACAGATACTCTGCATAAAGAGGAAATTCATAGCACAACATTGAATGTCCAACCTAAGT
CATCTCAGAATTATACATATATTGACTTTTTAATTCACATAGAATCTGGAACTCAACTGG
TTGGTTGGTTGGTGATGGGGTTGACCTGATTTCGGGAGAATTATTCCTGAAATAATTAAG
GAAATGGGGGTTCAATCTTTCTTCTTAGCTGCTTGTTCCTCCCCAGCACCTTCAACTTTC
TTCTCCTCCTCTTCAGCTTCCTTTGGTTTCCTCTCTTTTTTCACCTTCACCTCCTTCTTT
TTCTTTTTTTGCTTCTTAGACTCTTTCTTGGCAGTTTCTTCCTCTTCAGCTGCCTTCTCT
TCCTCGGCCTCTTCCTTGGCCTTCTCCTCCTTCTCGGCTTTTCCTTCAGAGGGGGGGCCC
ATCCTTGCCTTTCTTCACCTTGGAAGCCCTAATGGGTTCCCCCCATTTGGAACTGGCTCC
TCTTGGACTGGCCTTGGGCACCAATCACGGCAACGAACCGGTGGCCCCTCATAAGGGACC
TGGCCTGGCAAACCCCCGAAGCCACATCGGCCCAACAACTGGAAGTTTTGGAGGAACCCT
GTGATTTTGTTTCCCCACCTGT
Restriction Sites NotI-NotI     
ACCN NM_006158
Insert Size 2210 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation A TrueClone.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006158.1, NP_006149.1
RefSeq Size 3854 bp
RefSeq ORF 1635 bp
Locus ID 4747
Cytogenetics 8p21.2
Domains filament, filament_head
Protein Families Druggable Genome, ES Cell Differentiation/IPS
Protein Pathways Amyotrophic lateral sclerosis (ALS)
Gene Summary 'Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]'

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