Neurofilament (NEFL) (NM_006158) Human Untagged Clone

CAT#: SC116280

NEFL (untagged)-Human neurofilament, light polypeptide (NEFL)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NEFL
• Synonyms: CMT1F; CMT2E; CMTDIG; NF-L; NF68; NFL; PPP1R110
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006158, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGTTCCTTCAGCTACGAGCCGTACTACTCGACCTCCTACAAGCGGCGCTACGTGGAGACGCCCCGGG
  TGCACATCTCCAGCGTGCGCAGCGGCTACAGCACCGCACGCTCAGCTTACTCCAGCTACTCGGCGCCGGT
  GTCTTCCTCGCTGTCCGTGCGCCGCAGCTACTCCTCCAGCTCTGGATCGTTGATGCCCAGTCTGGAGAAC
  CTCGACCTGAGCCAGGTAGCCGCCATCAGCAACGACCTCAAGTCCATCCGCACGCAGGAGAAGGCGCAGC
  TCCAGGACCTCAATGACCGCTTCGCCAGCTTCATCGAGCGCGTGCACGAGCTGGAGCAGCAGAACAAGGT
  CCTGGAAGCCGAGCTGCTGGTGCTGCGCCAGAAGCACTCCGAGCCATCCCGCTTCCGGGCGCTGTACGAG
  CAGGAGATCCGCGACCTGCGCCTGGCGGCGGAAGATGCCACCAACGAGAAGCAGGCGCTCCAGGGCGAGC
  GCGAAGGGCTGGAGGAGACCCTGCGCAACCTGCAGGCGCGCTATGAAGAGGAGGTGCTGAGCCGCGAGGA
  CGCCGAGGGCCGGCTGATGGAAGCGCGCAAAGGCGCCGACGAGGCGGCGCTCGCTCGCGCCGAGCTCGAG
  AAGCGCATCGACAGCTTGATGGACGAAATCTCTTTTCTGAAGAAAGTGCACGAAGAGGAGATCGCCGAAC
  TGCAGGCGCAGATCCAGTACGCGCAGATCTCCGTGGAGATGGACGTGACCAAGCCCGACCTTTCCGCCGC
  GCTCAAGGACATCCGCGCGCAGTACGAGAAGCTGGCCGCCAAGAACATGCAGAACGCTGAGGAATGGTTC
  AAGAGCCGCTTCACCGTGCTGACCGAGAGCGCCGCCAAGAACACCGACGCCGTGCGCGCCGCCAAGGACG
  AGGTGTCCGAGAGCCGTCGTCTGCTCAAGGCCAAGACCCTGGAAATCGAAGCATGCCGGGGCATGAATGA
  AGCGCTGGAGAAGCAGCTGCAGGAGCTGGAGGACAAGCAGAACGCCGACATCAGCGCTATGCAGGACACG
  ATCAACAAATTAGAAAATGAATTGAGGACCACAAAGAGTGAAATGGCACGATACCTAAAAGAATACCAAG
  ACCTCCTCAACGTGAAGATGGCTTTGGATATTGAGATTGCAGCTTACAGGAAACTCTTGGAAGGCGAGGA
  GACCCGACTCAGTTTCACCAGCGTGGGAAGCATAACCAGTGGCTACTCCCAGAGCTCCCAGGTCTTTGGC
  CGATCTGCCTACGGCGGTTTACAGACCAGCTCCTATCTGATGTCCACCCGCTCCTTCCCGTCCTACTACA
  CCAGCCATGTCCAAGAGGAGCAGATCGAAGTGGAGGAAACCATTGAGGCTGCCAAGGCTGAGGAAGCCAA
  GGATGAGCCCCCCTCTGAAGGAGAAGCCGAGGAGGAGGAGAAGGACAAGGAAGAGGCCGAGGAAGAGGAG
  GCAGCTGAAGAGGAAGAAGCTGCCAAGGAAGAGTCTGAAGAAGCAAAAGAAGAAGAAGAAGGAGGTGAAG
  GTGAAGAAGGAGAGGAAACCAAAGAAGCTGAAGAGGAGGAGAAGAAAGTTGAAGGTGCTGGGGAGGAACA
  AGCAGCTAAGAAGAAAGATTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006158 unedited
  NGGTGCNAATTTGTATACGACCACTATAGGCGGCCGCGNAATCGGCACGAGGCATCCTCC
  CCCTTCCCTCTCTCCCCTGTCCTCTCTCTCCGGGCTCCCACCGCCGCCGCGGGCCGGGGA
  GCCACCGGCCGCCACCATGAGTTCCTTCAGCTACGAGCCGTACTACTCGACCTCCTACAA
  GCGGCGCTACGTGGAGACGCCCCGGGTGCACATCTCCAGCGTGCGCAGCGGCTACAGCAC
  CGCACGCTCAGCTTACTCCAGCTACTCGGCGCCGGTGTCTTCCTCGCTGTCCGTGCGCCG
  CAGCTACTCCTCCAGCTCTGGATCGTTGATGCCCAGTCTGGAGAACCTCGACCTGAGCCA
  GGTAGCCGCCATCAGCAACGACCTCAAGTCCATCCGCACGCAGGAGAAGGCGCAGCTCCA
  GGACCTCAATGACCGCTTCGCCAGCTTCATCGAGCGCGTGCACGAGCTGGAGCAGCAGAA
  CAAGGTCCTGGAAGCCGAGCTGCTGGTGCTGCGCCAGAAGCACTCCGAGCCATCCCGCTT
  CCGGGCGCTGTACGAGCAGGAGATCCGCGACCTGCGCCTGGCGGCGGAAGATGCCACCAA
  CGAGAAGCACGCGCTCCAGGGCGAGCGCGAAGGGCTGGAGGAGACCCTGCGCAACCTGCA
  GGCGCGCTATGAAGAGGAGGTGCTGAGCCCCGAGGACGCCGAAGGCCGGCTGATGGAAGC
  CCGCAAAGGCGCCGACGATGCGGCGCTCGCTCGCGCCGAGCTCCGAGAGCGCATGGACAG
  CTTGATGGACCAAATCTCTTTTCTGAAGACAGTGCCCGAAGAAGAGATCGCCCGACTGCC
  AGCGCAGATCCCATACCCCCACACCCCGGGCAGAGGACGTTGACAAGCCCGACCTACCGC
  CG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006158 unedited
  NTTTTAGCTCTGNNACCGCGGCCGCATNCTAGNGATCGGTTTTTTTTTTTTTTTTTTACA
  TCATTCGGTATAACTTTATTTACTATTTATTGCACATAACTCAGGGTCTTTAATTGCTAA
  CCACCGAAGGTTCAAAGGACTATTTTTCCATGAAGTTTAACATTAACATTAGTGAATTCA
  TTTACTCATGTGGTGTTTTTTATTGTAAACATCTGAATGATTCACATTGCCGTAGATCCT
  GAACTCATAAGCGTGGTCCATGCACAGGCTGGCAGCAAGCCAGAAAGCCACTCTGCAAGC
  AAACAGATACTCTGCATAAAGAGGAAATTCATAGCACAACATTGAATGTCCAACCTAAGT
  CATCTCAGAATTATACATATATTGACTTTTTAATTCACATAGAATCTGGAACTCAACTGG
  TTGGTTGGTTGGTGATGGGGTTGACCTGATTTCGGGAGAATTATTCCTGAAATAATTAAG
  GAAATGGGGGTTCAATCTTTCTTCTTAGCTGCTTGTTCCTCCCCAGCACCTTCAACTTTC
  TTCTCCTCCTCTTCAGCTTCCTTTGGTTTCCTCTCTTTTTTCACCTTCACCTCCTTCTTT
  TTCTTTTTTTGCTTCTTAGACTCTTTCTTGGCAGTTTCTTCCTCTTCAGCTGCCTTCTCT
  TCCTCGGCCTCTTCCTTGGCCTTCTCCTCCTTCTCGGCTTTTCCTTCAGAGGGGGGGCCC
  ATCCTTGCCTTTCTTCACCTTGGAAGCCCTAATGGGTTCCCCCCATTTGGAACTGGCTCC
  TCTTGGACTGGCCTTGGGCACCAATCACGGCAACGAACCGGTGGCCCCTCATAAGGGACC
  TGGCCTGGCAAACCCCCGAAGCCACATCGGCCCAACAACTGGAAGTTTTGGAGGAACCCT
  GTGATTTTGTTTCCCCACCTGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006158
• Insert Size: 2210 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: A TrueClone.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006158.1, NP_006149.1
• RefSeq Size: 3854 bp
• RefSeq ORF: 1635 bp
• Locus ID: 4747
• Cytogenetics: 8p21.2
• Domains: filament, filament_head
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Amyotrophic lateral sclerosis (ALS)
• Gene Summary: 'Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]'