Nrf2 (NFE2L2) (NM_006164) Human Untagged Clone

CAT#: SC116283

NFE2L2 (untagged)-Human nuclear factor (erythroid-derived 2)-like 2 (NFE2L2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NFE2L2
• Synonyms: HEBP1; IMDDHH; NRF2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116283 sequence for NM_006164 edited (data generated by NextGen Sequencing)
  ATGATGGACTTGGAGCTGCCGCCGCCGGGACTCCCGTCCCAGCAGGACATGGATTTGATT
  GACATACTTTGGAGGCAAGATATAGATCTTGGAGTAAGTCGAGAAGTATTTGACTTCAGT
  CAGCGACGGAAAGAGTATGAGCTGGAAAAACAGAAAAAACTTGAAAAGGAAAGACAAGAA
  CAACTCCAAAAGGAGCAAGAGAAAGCCTTTTTCGCTCAGTTACAACTAGATGAAGAGACA
  GGTGAATTTCTCCCAATTCAGCCAGCCCAGCACATCCAGTCAGAAACCAGTGGATCTGCC
  AACTACTCCCAGGTTGCCCACATTCCCAAATCAGATGCTTTGTACTTTGATGACTGCATG
  CAGCTTTTGGCGCAGACATTCCCGTTTGTAGATGACAATGAGGTTTCTTCGGCTACGTTT
  CAGTCACTTGTTCCTGATATTCCCGGTCACATCGAGAGCCCAGTCTTCATTGCTACTAAT
  CAGGCTCAGTCACCTGAAACTTCTGTTGCTCAGGTAGCCCCTGTTGATTTAGACGGTATG
  CAACAGGACATTGAGCAAGTTTGGGAGGAGCTATTATCCATTCCTGAGTTACAGTGTCTT
  AATATTGAAAATGACAAGCTGGTTGAGACTACCATGGTTCCAAGTCCAGAAGCCAAACTG
  ACAGAAGTTGACAATTATCATTTTTACTCATCTATACCCTCAATGGAAAAAGAAGTAGGT
  AACTGTAGTCCACATTTTCTTAATGCTTTTGAGGATTCCTTCAGCAGCATCCTCTCCACA
  GAAGACCCCAACCAGTTGACAGTGAACTCATTAAATTCAGATGCCACAGTCAACACAGAT
  TTTGGTGATGAATTTTATTCTGCTTTCATAGCTGAGCCCAGTATCAGCAACAGCATGCCC
  TCACCTGCTACTTTAAGCCATTCACTCTCTGAACTTCTAAATGGGCCCATTGATGTTTCT
  GATCTATCACTTTGCAAAGCTTTCAACCAAAACCACCCTGAAAGCACAGCAGAATTCAAT
  GATTCTGACTCCGGCATTTCACTAAACACAAGTCCCAGTGTGGCATCACCAGAACACTCA
  GTGGAATCTTCCAGCTATGGAGACACACTACTTGGCCTCAGTGATTCTGAAGTGGAAGAG
  CTAGATAGTGCCCCTGGAAGTGTCAAACAGAATGGTCCTAAAACACCAGTACATTCTTCT
  GGGGATATGGTACAACCCTTGTCACCATCTCAGGGGCAGAGCACTCACGTGCATGATGCC
  CAATGTGAGAACACACCAGAGAAAGAATTGCCTGTAAGTCCTGGTCATCGGAAAACCCCA
  TTCACAAAAGACAAACATTCAAGCCGCTTGGAGGCTCATCTCACAAGAGATGAACTTAGG
  GCAAAAGCTCTCCATATCCCATTCCCTGTAGAAAAAATCATTAACCTCCCTGTTGTTGAC
  TTCAACGAAATGATGTCCAAAGAGCAGTTCAATGAAGCTCAACTTGCATTAATTCGGGAT
  ATACGTAGGAGGGGTAAGAATAAAGTGGCTGCTCAGAATTGCAGAAAAAGAAAACTGGAA
  AATATAGTAGAACTAGAGCAAGATTTAGATCATTTGAAAGATGAAAAAGAAAAATTGCTC
  AAAGAAAAAGGAGAAAATGACAAAAGCCTTCACCTACTGAAAAAACAACTCAGCACCTTA
  TATCTCGAAGTTTTCAGCATGCTACGTGATGAAGATGGAAAACCTTATTCTCCTAGTGAA
  TACTCCCTGCAGCAAACAAGAGATGGCAATGTTTTCCTTGTTCCCAAAAGTAAGAAGCCA
  GATGTTAAGAAAAACTAG
  
  Clone variation with respect to NM_006164.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006164 unedited
  ATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGGCACGAGGGGGAGCCCGGAGGA
  GCCGCCGACGCAGCCGCCACCGCCGCCGCCGCCACCAGAGCCGCCCTGTCCGCGCCGCGC
  CTCGGCAGCCGGAACAGGGCCGCCGTCGGGGAGCCCCAACACACGGTCCACAGCTCATCA
  TGATGGACTTGGAGCTGCCGCCGCCGGGACTCCCGTCCCAGCAGGACATGGATTTGATTG
  ACATACTTTGGAGGCAAGATATAGATCTTGGAGTAAGTCGAGAAGTATTTGACTTCAGTC
  AGCGACGGAAAGAGTATGAGCTGGAAAAACAGAAAAAACTTGAAAAGGAAAGACAAGAAC
  AACTCCAAAAGGAGCAAGAGAAAGCCTTTTTCGCTCAGTTACAACTAGATGAAGAGACAG
  GTGAATTTCTCCCAATTCAGCCAGCCCAGCACATCCAGTCAGAAACCAGTGGATCTGCCA
  ACTACTCCCAGGTTGCCCACATTCCCAAATCAGATGCTTTGTACTTTGATGACTGCATGC
  AGCTTTTGGCGCAGACATTCCCGTTTGTAGATGACAATGAGGTTTCTTCGGCTACGTTTC
  AGTCACTTGTTCCTGATATTCCCGGTCACATCGAGAGCCCAGTCTTCATTGCTACTAATC
  AGGCTCAGTCACCTGAAACTTCTGTTGCTCAGGTAGCCCCTGTTGATTTAGACGGTATGC
  AACAGGACATTGNAGCAGTTGGNGAGGAGCTATTATCCATTCCTGAGTTACAGTGTCTTA
  ATATTGAAAATNGACAGCTGGTTGAGACTACCATGGTTNCNAGTCCAGAAGCCAAACTGA
  CAGAAAGTGACANTNATCATTTTTACTCATCTATACCCCTCATGGGANAAGAAGTANNGT
  ACTGTAGTCCACATTTTCTTATGCTTNTGAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006164 unedited
  NNNCCCTTCTATGNNACCGCGGCCGCTATCTAGNATCGAGTTTTTTTTTTTTTTTTTTTT
  TAACAGTCATAATAATCCTTTATTAGTACCAGCTCTTAAAATTTTTTTTTTTTGCCAGAG
  CTAAACAATTTAATATAAAAAATGCCATTTTTTGTCCATACAGTATTTATAAAAAAGTAC
  ATAGTGGTTAGTTTTGCAATAATTTCTTTTTAGCCAGATGTCATATCATCATATAAATCT
  ATGAATATAACAAATGACATAAGAACAGTATAAATAAGTTTTTGTAGTATTTACACTTAC
  ACAGAAACTAGCCCAAATGGTGTCCTAAGAAATTGTTTACAGTTAAAGTGAAACTACTGA
  TTCAACATACTGACACTCCAATGCTTTTTAAAGTTTCGTATTATTTTCTATACTAGTTTT
  GGCTATGATTTTGCATAGAATTACTTATAAAGTATGAGCATTTCACATCACAGTAGGAGC
  TTTTAGTATATTAGTACAAAAAACTAGCTCAGAAAGGTCAAATCTCCCTAATCTAGTTTT
  TCTAACATCTGGCTCCTACTTTGGGGACAGGAAAAACTTGCCTTTTTTGTTTGCTGCAGG
  AGGTTTCCCTTAGGAAAAAAGGGTTCCCTCTTACTCAGGAACAGGCTGAAAATTCGAGAA
  TAAGGGGCTGGTTGGTTTTTCCGGGGGGAAGGCTTTTGGAATTTTCCCTTTCTTTGGGGC
  AATTTTTTTTTTACTTTAAAAAGGCCAAAAACGGGCCAAGGCCCCAAAATTTCCAGGTTT
  CTTTTTTTGGGAATTGGAAGACCCTTTTTTTTTCCCCCCCCTCTTTTTCCAAATAAAGCA
  AGGGGGGCTTTGGGACCGCTTTGGGCAAATATTTTGAAAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006164
• Insert Size: 2600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006164.2, NP_006155.2
• RefSeq Size: 2439 bp
• RefSeq ORF: 1818 bp
• Locus ID: 4780
• Cytogenetics: 2q31.2
• Domains: BRLZ
• Protein Families: Transcription Factors
• Gene Summary: 'This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.