Nkx3.1 (NKX3-1) (NM_006167) Human Untagged Clone

CAT#: SC116287

NKX3 (untagged)-Human NK3 homeobox 1 (NKX3-1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NKX3-1
• Synonyms: BAPX2; NKX3; NKX3.1; NKX3A
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116287 sequence for NM_006167 edited (data generated by NextGen Sequencing)
  ATGCTCAGGGTTCCGGAGCCGCGGCCCGGGGAGGCGAAAGCGGAGGGGGCCGCGCCGCCG
  ACCCCGTCCAAGCCGCTCACGTCCTTCCTCATCCAGGACATCCTGCGGGACGGCGCGCAG
  CGGCAAGGCGGCCGCACGAGCAGCCAGAGACAGCGCGACCCGGAGCCGGAGCCAGAGCCA
  GAGCCAGAGGGAGGACGCAGCCGCGCCGGGGCGCAGAACGACCAGCTGAGCACCGGGCCC
  CGCGCCGCGCCGGAGGAGGCCGAGACGCTGGCAGAGACCGAGCCAGAAAGGCACTTGGGG
  TCTTATCTGTTGGACTCTGAAAACACTTCAGGCGCCCTTCCAAGGCTTCCCCAAACCCCT
  AAGCAGCCGCAGAAGCGCTCCCGAGCTGCCTTCTCCCACACTCAGGTGATCGAGTTGGAG
  AGGAAGTTCAGCCATCAGAAGTACCTGTCGGCCCCTGAACGGGCCCACCTGGCCAAGAAC
  CTCAAGCTCACGGAGACCCAAGTGAAGATATGGTTCCAGAACAGACGCTATAAGACTAAG
  CGAAAGCAGCTCTCCTCGGAGCTGGGAGACTTGGAGAAGCACTCCTCTTTGCCGGCCCTG
  AAAGAGGAGGCCTTCTCCCGGGCCTCCCTGGTCTCCGTGTATAACAGCTATCCTTACTAC
  CCATACCTGTACTGCGTGGGCAGCTGGAGCCCAGCTTTTTGGTAA
  
  Clone variation with respect to NM_006167.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006167 unedited
  GCCGCGAATTCGGCACCAGCGGGTGCATTCAGGCCAAAGCGGGGCCGCCGGGATGCTCAG
  GGTTCCGGAGCCGCGGCCCGGGGAGGCGAAAGCGGAGGGGGCCGCGCCGCCGACCCCGTC
  CAAGCCGCTCACGTCCTTCCTCATCCAGGACATCCTGCGGGACGGCGCGCAGCGGCAAGG
  CGGCCGCACGAGCAGCCAGAGACAGCGCGACCCGGAGCCGGAGCCAGAGCCAGAGCCAGA
  GGGAGGACGCAGCCGCGCCGGGGCGCAGAACGACCAGCTGAGCACCGGGCCCCGCGCCGC
  GCCGGAGGAGGCCGAGACGCTGGCAGAGACCGAGCCAGAAAGGCACTTGGGGTCTTATCT
  GTTGGACTCTGAAAACACTTCAGGCGCCCTTCCAAGGCTTCCCCAAACCCCTAAGCAGCC
  GCAGAAGCGCTCCCGAGCTGCCTTCTCCCACACTCAGGTGATCGAGTTGGAGAGGAAGTT
  CAGCCATCAGAAGTACCTGTCGGCCCCTGAACGGGCCCACCTGGCCAAGAACCTCAAGCT
  CACGGAGACCCAAGTGAAGATATGGGTTCCAGAACAGACGCTATANGACTAAGCGAAAGC
  AGCTCTCCTCGGAGCTGGGAGACTTGGAGAAGCACTCCTCNTTGGCCGCCCCTGAAGAGG
  ANGCCTTCTCCGGGGCCCTCCTGGGCTTCGTGTATACAGCTATCCTTACTACCCATACCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006167 unedited
  CCCTAGGTTGCCCGCGGCCGCAATCTACATTTTTTTTTTTTCTAATAAACACATGGGTAC
  TTTATTTTCAAAACACTCATATGTTGCTAAAAAACACATAGTAAAAATAAAGTTTGGTGG
  GGGTGCTGACTAAACTTCAAGTCACAGACTTTTATGTGACAGATTGGAGCAGGGTTTGTT
  ATGCATGTAGAGAACCCAAACTAATTTATTAAACAGGATAGAAACAGGCTGTCTGGGTGA
  AATGGTTCTGAGAACCATCCAATTCACCTGTCAGATGCTGATAGACTAGCTCTTCAGATG
  TTTTTCTACCAGTTCAGAGATGGGTTAATGACTAGTTCCAATGGGGAAAAAGCAAGATGG
  ATTCACAAACCAAGTAATTTTAAACAACGACACTTTTTTTTTTTTGCAACACAATATACA
  TCACAGTGAAATGTGTAATCCTTGCAAATTGCAAGTTGAAAGAATTAAATTCAGAGGAGG
  GGAGAGAAAGAGTATTCAGTAGGGACTGAGCACTAAATGCTTATTTTAAAAGAAATGTAA
  AGAGCAGAAAGCAATTCAGGCTACCCTGCCTTTTGCGCTGCCTAGCACTCCGGCCGGTGC
  CACCCGCCCGTGCCATCGAACATTCCCCTGCGCAGCCCCAAACTCCTAAACAACGGGGTC
  GAACACCGCACAAATCTCTAATTAACCTTATGCTGGCACTTTTCTCCTCCCCCCGCAAAC
  CTGCCCCAACTTCGTCAACCCACATTGCCCGCCTACTCCCTACACGCACATAATTTTCGG
  GCTCCCCAAACTTCCCGCCTCTACGCTCCTCCTTATTCCATGTCCTGTCCCCTAATATTC
  TCTCCCGCGTTCGCTTTCCCGCGTCCCTTGTATCCCCCCCCCCAAATATCAACCACCGCA
  CCCATCCCCTTGCCTCCTCCCCCTTCCTTCAAAACT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006167
• Insert Size: 2890 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006167.2, NP_006158.2
• RefSeq Size: 3266 bp
• RefSeq ORF: 705 bp
• Locus ID: 4824
• Cytogenetics: 8p21.2
• Domains: homeobox
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Prostate cancer
• Gene Summary: 'This gene encodes a homeobox-containing transcription factor. This transcription factor functions as a negative regulator of epithelial cell growth in prostate tissue. Aberrant expression of this gene is associated with prostate tumor progression. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jan 2012]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.