NSF (NM_006178) Human Untagged Clone

CAT#: SC116292

NSF (untagged)-Human N-ethylmaleimide-sensitive factor (NSF)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NSF
• Synonyms: SEC18; SKD2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116292 sequence for NM_006178 edited (data generated by NextGen Sequencing)
  ATGGCGGGCCGGAGCATGCAAGCGGCAAGATGTCCTACAGATGAATTATCTTTAACCAAT
  TGTGCAGTTGTGAATGAAAAGGATTTCCAGTCTGGCCAGCATGTGATTGTGAGGACCTCT
  CCCAATCACAGGTACACATTTACACTGAAGACACATCCATCGGTGGTTCCAGGGAGCATT
  GCATTCAGTTTACCTCAGAGAAAATGGGCTGGGCTTTCTATTGGGCAAGAAATAGAAGTC
  TCCTTATATACATTTGACAAAGCCAAACAGTGTATTGGCACAATGACCATCGAGATTGAT
  TTCCTGCAGAAAAAAAGCATTGACTCCAACCCTTATGACACCGACAAGATGGCAGCAGAA
  TTTATTCAGCAATTCAACAACCAGGCCTTCTCAGTGGGACAACAGCTTGTCTTTAGCTTC
  AATGAAAAGCTTTTTGGCTTACTGGTGAAGGACATTGAAGCCATGGATCCTAGCATCCTG
  AAGGGAGAGCCTGCGACAGGGAAAAGGCAGAAGATTGAAGTAGGACTGGTTGTTGGAAAC
  AGTCAAGTTGCATTTGAAAAAGCAGAAAATTCGTCACTTAATCTTATTGGCAAAGCTAAA
  ACCAAGGAAAATCGCCAATCAATTATCAATCCTGACTGGAACTTTGAAAAAATGGGAATA
  GGAGGTCTAGACAAGGAATTTTCAGATATTTTCCGACGAGCATTTGCTTCCCGAGTATTT
  CCTCCAGAGATTGTGGAGCAGATGGGTTGTAAACATGTTAAAGGCATCCTGTTATATGGA
  CCCCCAGGTTGTGGTAAGACTCTCTTGGCTCGACAGATTGGCAAGATGTTGAATGCAAGA
  GAGCCCAAAGTGGTCAATGGGCCAGAAATCCTTAACAAATATGTGGGAGAATCAGAGGCT
  AACATTCGCAAACTTTTTGCTGATGCTGAAGAGGAGCAAAGGAGGCTTGGTGCTAACAGT
  GGTTTGCACATCATCATCTTTGATGAAATTGATGCCATCTGCAAGCAGAGAGGGAGCATG
  GCTGGTAGCACGGGAGTTCATGACACTGTTGTCAACCAGTTGCTGTCCAAAATTGATGGC
  GTGGAGCAGCTAAACAACATCCTAGTCATTGGAATGACCAATAGACCAGATCTGATAGAT
  GAGGCTCTTCTTAGACCTGGAAGACTGGAAGTTAAAATGGAGATAGGCTTGCCAGATGAG
  AAAGGCCGACTACAGATTCTTCACATCCACACAGCAAGAATGAGAGGGCATCAGTTACTC
  TCTGCTGATGTAGACATTAAAGAACTGGCCGTGGAGACCAAGAATTTCAGTGGTGCTGAA
  TTGGAGGGTCTGGTGCGAGCAGCCCAGTCCACTGCTATGAATAGACACATAAAGGCCAGT
  ACTAAAGTGGAAGTGGACATGGAGAAAGCAGAAAGCCTGCAAGTGACGAGAGGAGACTTC
  CTTGCTTCTTTGGAGAATGATATCAAACCAGCCTTTGGCACAAACCAAGAAGATTATGCA
  AGTTACATTATGAACGGTATCATCAAATGGGGTGACCCAGTTACTCGAGTTCTAGATGAT
  GGGGAGCTGCTGGTGCAGCAGACTAAGAACAGTGACCGCACACCATTGGTCAGCGTGCTT
  CTGGAAGGCCCTCCTCACAGTGGGAAGACTGCTTTAGCTGCAAAAATTGCAGAGGAATCC
  AACTTCCCGTTCATCAAGATCTGTTCTCCTGATAAAATGATTGGCTTTTCTGAAACAGCC
  AAATGTCAGGCCATGAAGAAGATCTTTGATGATGCGTACAAATCCCAGCTCAGTTGTGTG
  GTTGTGGATGACATTGAGAGATTGCTTGATTACGTCCCTATTGGCCCTCGATTTTCAAAT
  CTTGTATTACAGGCTCTTCTCGTTTTACTGAAAAAGGCACCTCCTCAGGGCCGCAAGCTT
  CTTATCATTGGGACCACTAGCCGCAAAGATGTCCTTCAGGAGATGGAAATGCTTAACGCT
  TTCAGCACCACCATCCACGTGCCCAACATTGCCACAGGAGAGCAGCTGTTGGAAGCTTTG
  GAGCTTTTGGGCAACTTCAAGGATAAGGAACGCACCACAATTGCACAGCAAGTCAAAGGG
  AAGAAGGTCTGGATAGGAATCAAGAAGTTACTAATGCTGATCGAGATGTCCCTACAGATG
  GATCCTGAATACCGTGTGAGAAAATTCTTGGCCCTCTTAAGAGAAGAAGGAGCTAGCCCC
  CTTGATTTTGATTGA
  
  Clone variation with respect to NM_006178.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006178 unedited
  TCTTTTTGTATACGACTTATCTATAGGGCGGCCGCGAATTCGCACGAGGCGGACGTGTCC
  GCGAAGAGGCGGGCCGGAGCATGCAAGCGGCAAGATGTCCTACAGATGAATTATCTTTAA
  CCAATTGTGCAGTTGTGAATGAAAAGGATTTCCAGTCTGGCCAGCATGTGATTGTGAGGA
  CCTCTCCCAATCACAGGTACACATTTACACTGAAGACACATCCATCGGTGGTTCCAGGGA
  GCATTGCATTCAGTTTACCTCAGAGAAAATGGGCTGGGCTTTCTATTGGGCAAGAAATAG
  AAGTCTCCTTATATACATTTGACAAAGCCAAACAGTGTATTGGCACAATGACCATCGAGA
  TTGATTTCCTGCAGAAAAAAAGCATTGACTCCAACCCTTATGACACCGACAAGATGGCAG
  CAGAATTTATTCAGCAATTCAACAACCAGGCCTTCTCAGTGGGACAACAGCTTGTCTTTA
  GCTTCAATGAAAAGCTTTTTGGCTTACTGGTGAAGGACATTGAAGCCATGGATCCTAGCA
  TCCTGAAGGGAGAGCCTGCGACAGGGAAAAGGCAGAAGATTGAAGTACGACTGGTTGTTG
  GAAACAGTCAAGTTGCATTTGAAAAAGCAGAAAATTCGTCACTTAATCTTATTGGCAAAG
  CTAAACCCAGGAAAATCGCCCATCAATTATCAATCCTGACTGGAACTTTTGAAAAAGGGA
  ATAGGAGGTCTAGACCAGGAATTTTAGATATCTTCCGACAAGCATTTGCTTTCCGAGTAT
  TTCCTTCCAAAATTGTGGAGCAGAAGGGGTTGTACACATGGTAACAGGCATCCTGTTTTT
  TGGACCCCCCAGCTGGCGGCAAGACTCTCTTGCTTCCACCAATTGGCCAGAGTTGAATGC
  AAGAAAGCCCAAGTGGTCATGGGGCTCAAATTCTTAACCAAATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006178 unedited
  TTTTTTTCAAGTATATTTCNACTCNCTNTNTNNATNTGGGCATTNCCTTCATTTGCATTA
  AACAATATTTTTTCAATACAGTTTTGGACAAAACACAAAGACATTAAGCCTCATTTTAAC
  AAGAGACATAAGTTAACACAATGTGTGCTGCTTTTCATGAGGAGGAAAGAGGCAANNNNN
  NNATNNCCTTTAGAGGAATTCCAGGATACTGGCCACNCCCNNANNNGAATCACAGGATCN
  CTTTNNNNNNNAATACAATCNCCCCAATTTTTTTCNCCCCTTTTTAANNAAAGACCCCAA
  AATAAGCTAAGGAAGAAAACCCAAACAAAGAAGAATTGACCTCCAAGTCTCCCCAAAAGT
  ATACAAATGGCAAGATTTGGAGATGATCTGCTTTCTCACATGAGGACAAAATACAGAGGA
  GCCCCACCCAAGTGCCACTGTGGCCACAAGCCTCATGGGTGGCGTGTGAGGTAAGCACCT
  TNAGATGGCTTCTAATTGCTTGTCCTTGCATTTTAAGCAGCAGTTGGGAGAGAGAGCACA
  CTCCATAAGTCTCATCACCAAATATGACCTAATTAAATTCCTGCTAAGTCAGTCAGTTGT
  AAGACAAATCACCCTCAAAAGGACTCAACGGGTGCTTCCTTTTGGAAGAAGTGTGTGNAG
  GGTAAAGAAATGTGTTCAATGTATTGGAATGGACAATAAAGGACTGAAGGAGAAGCCTAC
  AAAATGATTGATTTTTTCTGGAAATTAATGTTTTAAACCCTAGATAGTCCAGATCATTTA
  AAATAAAGGATAATTCTCAATAATCTTTAACCTAGGAAAAAACCGTGGTTCTAGTTAGGC
  CTAATTTTTTCCTTAGGAATGCCCCCTTTGAATGGGCACCTATTAGGCTGGGGAGTTGGA
  AATACTTTAAAACCCTTTTTTTTGGGGGGGATGCCTTCCCTCCCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006178
• Insert Size: 4260 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006178.1, NP_006169.1
• RefSeq Size: 3960 bp
• RefSeq ORF: 2235 bp
• Locus ID: 4905
• Cytogenetics: 17q21.31
• Domains: cdc48_N, AAA, AAA, cdc48_2
• Protein Families: Protease
• Gene Summary: ''
  Transcript Variant: This variant (1) represents the longer transcript and encodes the protein.