Nurr1 (NR4A2) (NM_006186) Human Untagged Clone

CAT#: SC116298

NR4A2 (untagged)-Human nuclear receptor subfamily 4, group A, member 2 (NR4A2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NR4A2
• Synonyms: HZF-3; NOT; NURR1; RNR1; TINUR
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006186, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCTTGTGTTCAGGCGCAGTATGGGTCCTCGCCTCAAGGAGCCAGCCCCGCTTCTCAGAGCTACAGTT
  ACCACTCTTCGGGAGAATACAGCTCCGATTTCTTAACTCCAGAGTTTGTCAAGTTTAGCATGGACCTCAC
  CAACACTGAAATCACTGCCACCACTTCTCTCCCCAGCTTCAGTACCTTTATGGACAACTACAGCACAGGC
  TACGACGTCAAGCCACCTTGCTTGTACCAAATGCCCCTGTCCGGACAGCAGTCCTCCATTAAGGTAGAAG
  ACATTCAGATGCACAACTACCAGCAACACAGCCACCTGCCCCCCCAGTCTGAGGAGATGATGCCGCACTC
  CGGGTCGGTTTACTACAAGCCCTCCTCGCCCCCGACGCCCACCACCCCGGGCTTCCAGGTGCAGCACAGC
  CCCATGTGGGACGACCCGGGATCTCTCCACAACTTCCACCAGAACTACGTGGCCACTACGCACATGATCG
  AGCAGAGGAAAACGCCAGTCTCCCGCCTCTCCCTCTTCTCCTTTAAGCAATCGCCCCCTGGCACCCCGGT
  GTCTAGTTGCCAGATGCGCTTCGACGGGCCCCTGCACGTCCCCATGAACCCGGAGCCCGCCGGCAGCCAC
  CACGTGGTGGACGGGCAGACCTTCGCTGTGCCCAACCCCATTCGCAAGCCCGCGTCCATGGGCTTCCCGG
  GCCTGCAGATCGGCCACGCGTCTCAGCTGCTCGACACGCAGGTGCCCTCACCGCCGTCGCGGGGCTCCCC
  CTCCAACGAGGGGCTGTGCGCTGTGTGTGGGGACAACGCGGCCTGCCAACACTACGGCGTGCGCACCTGT
  GAGGGCTGCAAAGGCTTCTTTAAGCGCACAGTGCAAAAAAATGCAAAATACGTGTGTTTAGCAAATAAAA
  ACTGCCCAGTGGACAAGCGTCGCCGGAATCGCTGTCAGTACTGCCGATTTCAGAAGTGCCTGGCTGTTGG
  GATGGTCAAAGAAGTGGTTCGCACAGACAGTTTAAAAGGCCGGAGAGGTCGTTTGCCCTCGAAACCGAAG
  AGCCCACAGGAGCCCTCTCCCCCTTCGCCCCCGGTGAGTCTGATCAGTGCCCTCGTCAGGGCCCATGTCG
  ACTCCAACCCGGCTATGACCAGCCTGGACTATTCCAGGTTCCAGGCGAACCCTGACTATCAAATGAGTGG
  AGATGACACCCAGCATATCCAGCAATTCTATGATCTCCTGACTGGCTCCATGGAGATCATCCGGGGCTGG
  GCAGAGAAGATCCCTGGCTTCGCAGACCTGCCCAAAGCCGACCAAGACCTGCTTTTTGAATCAGCTTTCT
  TAGAACTGTTTGTCCTTCGATTAGCATACAGGTCCAACCCAGTGGAGGGTAAACTCATCTTTTGCAATGG
  GGTGGTCTTGCACAGGTTGCAATGCGTTCGTGGCTTTGGGGAATGGATTGATTCCATTGTTGAATTCTCC
  TCCAACTTGCAGAATATGAACATCGACATTTCTGCCTTCTCCTGCATTGCTGCCCTGGCTATGGTCACAG
  AGAGACACGGGCTCAAGGAACCCAAGAGAGTGGAAGAACTGCAAAACAAGATTGTAAATTGTCTCAAAGA
  CCACGTGACTTTCAACAATGGGGGGTTGAACCGCCCCAATTATTTGTCCAAACTGTTGGGGAAGCTCCCA
  GAACTTCGTACCCTTTGCACACAGGGGCTACAGCGCATTTTCTACCTGAAATTGGAAGACTTGGTGCCAC
  CGCCAGCAATAATTGACAAACTTTTCCTGGACACTTTACCTTTCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006186 unedited
  ACCTGGTTTTGTAAACCCCCCTCCTAGTAGGGCGGCAAACCCATTCGGCACGAGGGGCGG
  CCAGGGCCAGTCCGCCCGGCGGCTCGCGCACGGCTCCGCGGTCCCTTTTGCCTGTCCAGC
  CGGCCGCCTGTCCCTGCTCCCTCCCTCCGTGAGGTGTCCGGGTTCCCTTCGCCCAGCTCT
  CCCACCCCTACCCGACCCCGGCGCCCGGGCTCCCTTTTTGAACTGCACTTCGGCAGAGTT
  GAATGAATGAAGAGAGACGCGGAGAACTCCTAAGGAGGAGATTGGACAGGCTGGACTCCC
  CATTGCTTTTCTAAAAATCTTGGAAACTTTGTCCTTCATTGAATTACGACACTGTCCACC
  TTTAATTTCCTCGAAAACGCCTGTAACTCGGCTGAAGCCATGCCTTGTGTTCAGGCGCAG
  TATGGGTCCTCGCCTCAAGGAGCCAGCCCCGCTTCTCAGAGCTACAGTTACCACTCTTCG
  GGAGAATACAGCTCCGATTTCTTAACTCCAGAGTTTGTCAAGTTTAGCATGGACCTCACC
  AACACTGAAATCACTGCCACCACTTCTCTCCCCAGCTTCAGTACCTTTATGGACAACTAC
  AGCACAGGCTACGACGTCAAGCCACCTTGCTTGTACCAAATGCCCCTGTCCGGACAGCAG
  TCCTCCATTAAGGTAGAAGACATTCAGATGCACATCTACCATGCACACAGCCACCTGCCC
  CCCCAGTCTGATGAGATGATGCCGCACTCCCGGTCCGTTTACTACAAGCCCTCCTTGCCC
  CCGACGCCCACCACCCCGGGCTTCCAGTGCAGCACAGNCCCCTGTGGGACGACCCGGGAT
  CTCTCCACAACTTCCACCAGACTACGTGCCCCCTACGCCATGATCGAGCAGAAGAAACGC
  CGTCTCCCGCCTTTCCTTTTTCTCCTAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006186 unedited
  TCTAGAGTCGAGNNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAT
  TTGGAATATTTATTCATAAATAGGCACAAAATAATTTAAAAAGCCAAACTGGATTGGGGA
  AATTTACAAGCCTTTGCCTTTTTCAACTCATGCCACCCACGCAACATTTAGTTTTACTAT
  ATATTACAGCTTTCTTTACAGCAAAAAACTTTGAAGTCTTGTTAAAGGGCAATACTTGGG
  GGGCCCCTTAAAAAGGGTCTCTGGGGGTGTGTGCGTGTGTATGTGTGTGGGCGTTACATT
  TGGCTGAACTGCAACAACCAAGCATGGCCAAACATTTCCCATTATACCTTATAGCAATCT
  TTCTTCTGAACAACAAACTGGTTTTTAAACTGAGAATAAAAAGTTTATACCACTGGATTG
  TGTGGAGCCCATGTTCTAAATCCAGGATGCCCCGGAGCCAAAATGCCCTTTCAGGTTCTG
  CCTGCAGGTTAGGAAAACCAACAGTTTTGGGTTGTGTGGTTGTCTTCTTTAAGGATCAAG
  GGGGCTAGGAGGGTTACANAAATGGGGGGGCACCTTGAGCTGAAACTGCTCACACGGCTA
  TCTTTGCCCATGTGACTTGCCCCCTTTTGACAGTTTCCACTATCATTCCAGTCTCCTTTG
  AAGCGCTGGGAAGGAGGCTTAAAAAGGAAAGTGTCCAGAAAAAGTTGGCATTATTGGTGG
  GGGGGGCCCCAACCTTCCAATTTCAGGTAAAAATGGCGCGACCCCCCTGGGGGCAAAGGG
  TCCAAATTTTGGGGAGCTCCTCACACAGTTGGAACATAAATTGGGGCCGGGCAACCCCCC
  ATTGGGGAAAGACCAGGGGCGTTTGAAAAATTTACAATTTGTTTTGGGAANTTTCCCCTC
  TTGGGGGGCCTTGACCGCGGCTCTTTGTGCCCATTCCGCGGGCCATGCCGAGAAGGGCAA
  ATGTCAGGTTATTCTGACGTGGGGGGAGATCACATGGGATCTTCTCTTCCCGGCCCGACG
  CT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006186
• Insert Size: 2870 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006186.2, NP_006177.1
• RefSeq Size: 3546 bp
• RefSeq ORF: 3546 bp
• Locus ID: 4929
• Cytogenetics: 2q24.1
• Domains: HOLI, zf-C4
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Gene Summary: 'This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. The encoded protein may act as a transcription factor. Mutations in this gene have been associated with disorders related to dopaminergic dysfunction, including Parkinson disease, schizophernia, and manic depression. Misregulation of this gene may be associated with rheumatoid arthritis. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]'