PCBP1 (NM_006196) Human Untagged Clone

CAT#: SC116302

PCBP1 (untagged)-Human poly(rC) binding protein 1 (PCBP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PCBP1
• Synonyms: HEL-S-85; hnRNP-E1; hnRNP-X; HNRPE1; HNRPX
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116302 sequence for NM_006196 edited (data generated by NextGen Sequencing)
  ATGGATGCCGGTGTGACTGAAAGTGGACTAAATGTGACTCTCACCATTCGGCTTCTTATG
  CACGGAAAGGAAGTAGGAAGCATCATTGGGAAGAAAGGGGAGTCGGTTAAGAGGATCCGC
  GAGGAGAGTGGCGCGCGGATCAACATCTCGGAGGGGAATTGTCCGGAGAGAATCATCACT
  CTGACCGGCCCCACCAATGCCATCTTTAAGGCTTTCGCTATGATCATCGACAAGCTGGAG
  GAAGATATCAACAGCTCCATGACCAACAGTACCGCGGCCAGCAGGCCCCCGGTCACCCTG
  AGGCTGGTGGTGCCGGCCACCCAGTGCGGCTCCCTGATTGGGAAAGGCGGGTGTAAGATC
  AAAGAGATCCGCGAGAGTACGGGGGCGCAGGTCCAGGTGGCGGGGGATATGCTGCCCAAC
  TCCACCGAGCGGGCCATCACCATCGCTGGCGTGCCGCAGTCTGTCACCGAGTGTGTCAAG
  CAGATTTGCCTGGTCATGCTGGAGACGCTCTCCCAGTCTCCGCAAGGGAGAGTCATGACC
  ATTCCGTACCAGCCCATGCCGGCCAGCTCCCCAGTCATCTGCGCGGGCGGCCAAGATCGG
  TGCAGCGACGCTGCGGGCTACCCCCATGCCACCCATGACCTGGAGGGACCACCTCTAGAT
  GCCTACTCGATTCAAGGACAACACACCATTTCTCCGCTCGATCTGGCCAAGCTGAACCAG
  GTGGCAAGACAACAGTCTCACTTTGCCATGATGCACGGCGGGACCGGATTCGCCGGAATT
  GACTCCAGCTCTCCAGAGGTGAAAGGCTATTGGGCAAGTTTGGATGCATCTACTCAAACC
  ACCCATGAACTCACCATTCCAAATAACTTAATTGGCTGCATAATCGGGCGCCAAGGCGCC
  AACATTAATGAGATCCGCCAGATGTCCGGGGCCCAGATCAAAATTGCCAACCCAGTGGAA
  GGCTCCTCTGGTAGGCAGGTTACTATCACTGGCTCTGCTGCCAGTATTAGTCTGGCCCAG
  TATCTAATCAATGCCAGGCTTTCCTCTGAGAAGGGCATGGGGTGCAGCTAG
  
  Clone variation with respect to NM_006196.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006196 unedited
  TCACTATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCGCCGCT
  CGCCATGGATGCCGGTGTGACTGAAAGTGGACTAAATGTGACTCTCACCATTCGGCTTCT
  TATGCACGGAAAGGAAGTAGGAAGCATCATTGGGAAGAAAGGGGAGTCGGTTAAGAGGAT
  CCGCGAGGAGAGTGGCGCGCGGATCAACATCTCGGAGGGGAATTGTCCGGAGAGAATCAT
  CACTCTGACCGGCCCCACCAATGCCATCTTTAAGGCTTTCGCTATGATCATCGACAAGCT
  GGAGGAAGATATCAACAGCTCCATGACCAACAGTACCGCGGCCAGCAGGCCCCCGGTCAC
  CCTGAGGCTGGTGGTGCCGGCCACCCAGTGCGGCTCCCTGATTGGGAAAGGCGGGTGTAA
  GATCAAAGAGATCCGCGAGAGTACGGGGGCGCAGGTCCAGGTGGCGGGGGATATGCTGCC
  CAACTCCACCGAGCGGGCCATCACCATCGCTGGCGTGCCGCAGTCTGTCACCGAGTGTGT
  CAAGCAGATTTGCCTGGTCATGCTGGAGACGCTCTCCCAGTCTCCGCAAGGGAGAGTCAT
  GACCATTCCGTACCAGCCCATGCCGGCCAGCTCCCCAGTCATCTGCGCGGGCGGCCAAGA
  TCGGTGCAGCGACGCTGCGGGCTACCCCCATGCCACCCATGACCTGNAGGGACCACCTCT
  AGATGCCTACTCGATTCAGGGACACACACCATTTCTCCGCTCGATCTGGCCAAGCTGAAC
  CCAGTGGCAAGAAAACAGTCTCACTTTGCCATGATGCCACGCGGNACCGGATTCGCCGNA
  ATTTGACTCCACTCTCCACAAGTGAAAGGCTATTGCGGCCAGTTGGATGCCTCTACTCAA
  ACACNCATGAACCTCCCATTTCCAATAATCAATTGCTT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006196 unedited
  CCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAACCAATCCGTTTTTTAATTCTTA
  AATTCTTGACATTACAGAACTAAACTGAAATTTATTAACATTCCACTCTTACATTTCTTA
  TCGACAAACAGAAATAAAATTCATGAGCCAAAAAACCCAAAACAAAACTAAAAACAGGGA
  AAAGCTTATAAAACTAAATATGGATCCCAGCATTAACAGCTGAACAGAGAATGTGATTTT
  TAAATTCTTAGCGGATGATAAAGTTGTGTAGAAACTGAACACTTACAAATTATTTAAAAC
  CTGGAATCACTGACCAGAAATTACACAGTTGGATCATGGGAGAACAGCAGAAAGGGGTTA
  TTGAGGGAACCTACACTGTTCTAGCTGCACCCCATGCCCTTCTCAGAGGAAAGCCTGGCA
  TTGATTAGATACTGGGCCAGACTAATACTGGCAGCAGAGCCAGTGATAGTAACCTGCCTA
  CCAGAGGAGCCTTCCACTGGGTTGGCAATTTTGATCTGGGCCCCGGACATCTGGCGGATC
  TCATTAATGTTGGCGCCTTGGCGCCCGATTATGCAGCCAATTAAGTTATTTGGAATGGTG
  AGTTCATGGGTGGTTTGAGTAGATGCATCCAAACTTGCCCAATAGCCTTTCACCTCTGGA
  GAGCTGGAGTCAATTCCGGCGAATCCGGTCCCGCCGTGCATCATGGGCAAGTGAGACTGG
  TGTCTTGCCACCTGGTTCAGCTTGGCCAGATCGAGCGGAGAAAGGTGGGGTNGTCCTTGA
  ATCGAGTAGGCATCTAGAGGGTGGTCCCTNNCAGTCATGGGTGGGCATGGGGGTANCCCG
  CANCGTCNCTGCACCGATCTTGGCCGCCGCGCAAATACTGGGGAACTGGNCCGCATGGGC
  TGGACNGAAATGGCATGACTTTCCCTTGCGAGACTGGGGAAAGCGTTCCACATGACAGGC
  AATTTGCTGACAACTTGTGACGATGGGGACGCACGAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006196
• Insert Size: 1470 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006196.2, NP_006187.1
• RefSeq Size: 1634 bp
• RefSeq ORF: 1071 bp
• Locus ID: 5093
• Cytogenetics: 2p13.3
• Domains: KH
• Protein Pathways: Spliceosome
• Gene Summary: 'This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq, Jul 2008]'