CAPZA1 (NM_006135) Human Untagged Clone

CAT#: SC116306

CAPZA1 (untagged)-Human capping protein (actin filament) muscle Z-line, alpha 1 (CAPZA1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CAPZA1
• Synonyms: CAPPA1; CAPZ; CAZ1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116306 sequence for NM_006135 edited (data generated by NextGen Sequencing)
  ATGGCCGACTTCGATGATCGTGTGTCGGATGAGGAGAAGGTACGCATAGCTGCTAAATTC
  ATCACTCATGCACCCCCAGGGGAATTTAATGAAGTATTCAATGACGTTCGGCTACTACTT
  AATAATGACAATCTCCTCAGGGAAGGGGCAGCACATGCATTTGCCCAGTATAACATGGAT
  CAGTTCACGCCTGTGAAGATAGAAGGATATGAAGATCAGGTCTTAATTACAGAGCACGGT
  GACCTGGGTAATAGCAGATTTTTAGATCCAAGAAACAAAATTTCCTTTAAATTTGACCAC
  TTACGGAAAGAAGCAAGTGACCCCCAGCCAGAAGAAGCAGATGGAGGTCTGAAGTCTTGG
  AGAGAATCCTGTGACAGTGCTTTAAGAGCCTATGTGAAAGACCATTATTCCAACGGCTTC
  TGTACTGTTTATGCTAAAACTATCGATGGGCAACAGACTATTATTGCATGTATTGAAAGC
  CACCAGTTTCAGCCTAAAAACTTCTGGAATGGTCGTTGGAGATCAGAGTGGAAGTTCACC
  ATCACACCACCTACAGCCCAGGTGGTTGGCGTGCTTAAGATTCAGGTTCACTATTATGAA
  GATGGCAATGTTCAGTTGGTTAGTCATAAAGATGTACAGGATTCACTAACTGTTTCGAAT
  GAAGCCCAAACTGCCAAGGAGTTTATTAAAATCATAGAGAATGCAGAAAATGAGTATCAG
  ACAGCAATTAGTGAAAACTATCAAACAATGTCAGATACCACATTCAAGGCCTTGCGCCGG
  CAGCTTCCAGTTACCCGCACCAAAATCGACTGGAACAAGATACTCAGCTACAAGATTGGC
  AAAGAAATGCAGAATGCTTAA
  
  Clone variation with respect to NM_006135.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006135 unedited
  CTATAAGTCGGCCGCGAAATCGCACGAGTCCTCGTGCCGAATTCGGCACGAGCCTCGTGC
  CGAATTCGGCACGAGGCCCAAGATGGCCGACTTCGATGATCGTGTGTCGGATGAGGAGAA
  GGTACGCATAGCTGCTAAATTCATCACTCATGCACCCCCAGGGGAATTTAATGAAGTATT
  CAATGACGTTCGGCTACTACTTAATAATGACAATCTCCTCAGGGAAGGGGCAGCACATGC
  ATTTGCCCAGTATAACATGGATCAGTTCACGCCTGTGAAGATAGAAGGATATGAAGATCA
  GGTCTTAATTACAGAGCACGGTGACCTGGGTAATAGCAGATTTTTAGATCCAAGAAACAA
  AATTTCCTTTAAATTTGACCACTTACGGAAAGAAGCAAGTGACCCCCAGCCAGAATAAGC
  AGATGGAGGTCTGAAATCTTGGAGAGAATCCTGTGACAGTGCTTTAAGAGCCTATGTGAA
  AGACCATTATTCCAACGGCTTCTGTACTGTTTATGCTACAACTATCGATGGGCAACAGAC
  TATTATTGCATGTATTGACAGCCACCAGTTTCAGCCTAACAACTTCTGCAATGGTCGTTG
  GAGATCAGAGTGGAAGTTCACCATCACACCACCTACAGCGCANGTGTCTGGCGTGCTTAA
  GATTCACGCTCACTATTATGAAGATGGCAATGTTCAATTGGCTACTCCTAAAGATGTCAG
  GATTCACTAACTGTTGCAATGAAGCCCAACTGCCAAGAGTTTATTAAATTCTAGAGATGC
  CGAAATGATTTAGGCCGTATTCCTGAAACTATCAAACATGCCCATACACATTGAAGCCTT
  GCGCGGCACTTCCATGTCCCCCACCAAATTGCTGGAGCAGAACTCAGTCCAAAATGGAAG
  AATGCAAGAGCTTAAGGCTGAGTAGAACTTTATATGTGAACACAAGATTACGGGTGGCTT
  GAAAAACCGATTTAACCCAGGAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006135 unedited
  GGCCGCAATCTAGAGTCGAGNNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA
  AAACAAAACAGGTATATTTCTATTAAACTACCTGATACAAAAAATAGTGGGGTATGGATG
  GATAGTATGAAGGACAAATAATACAAATTTTTTTTATTGAAATAAACAAAAATGCATACA
  CAGCTCAATGGGTCACCTGGAACAAACTTGCTTGACTATATTACTGAGCAAAAACAAAAC
  GGAAGCACAAACACCTTTTTTTTTTTGACATGTATCTGGAAATAGGTAGGAAAACACTAC
  TTGGTTTTTTAAAAAACTGACCTTCCCTTAAGGCCTGGTCATAAAAGTGTAAACAATGTA
  AATGAATCCACCATTACCAGTTGTCATATCATATCTATGTCACCTGTGTATTCTGAAATT
  ACACACATACCTGCCAATATACCTGGGAAAGGTTATTTTATCACAGTTACACTTGAGTTC
  TTGGCAGGCAGGACTGAGGAAGAGTAATTTGAAAGAAGTTTTACATCCTATTTAGAAGAA
  ATCACTAGTATTTCCTTAAAAACAGGTTACAATAGAAAGATACTGCCTGGAAGTTATCCT
  TTCACTTTGGNTCATTTTTAGTTTTTCTTTATGATTTACATAGCCTGTTATTCATTTTGC
  TATACACAATCCTGCCATAAAGATTAAGCACAGATACTATTATTCCTTCACATCTGCATT
  TTTAAGGTTTATACTCTAATNCACAGTACGTCAGAAGTTTTTGATGTTAAAAAAATAAAT
  GGTAAAATCGCTCTAAAAAATGCAATGTTTATAACTCCATGGTCCTAAATGGAANGGACA
  ATGANAGAAGGGATAAAGCAAACTGTCCTTGGGCAGGAAATGGGTTCAGTTCTTGATATT
  AAAACAATTCCCAAAGAAATTGCCCTCCTTTGCCTCCAAAAGAACTGAAAGTCCCATCCA
  ACTTTAGAAATAGTTTGGAAGCCTAATGAGATCAAGCCCCAAGGGAAAGATATTGGTTTT
  CATTATTAAAACGNAAAACCTCTTAGGTTGGGGAACAAAA
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_006135
• Insert Size: 2550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006135.1, NP_006126.1
• RefSeq Size: 2385 bp
• RefSeq ORF: 861 bp
• Locus ID: 829
• Cytogenetics: 1p13.2
• Domains: F-actin_cap_A
• Gene Summary: 'CAPZA1 is a member of the F-actin capping protein alpha subunit family. This gene encodes the alpha subunit of the barbed-end actin binding protein. The protein regulates growth of the actin filament by capping the barbed end of growing actin filaments. [provided by RefSeq, Jul 2008]'