MT3 (NM_005954) Human Untagged Clone

CAT#: SC116395

MT3 (untagged)-Human metallothionein 3 (MT3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MT3
• Synonyms: GIF; GIFB; GRIF; ZnMT3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116395 sequence for NM_005954 edited (data generated by NextGen Sequencing)
  ATGGACCCTGAGACCTGCCCCTGCCCTTCTGGTGGCTCCTGCACCTGCGCGGACTCCTGC
  AAGTGCGAGGGATGCAAATGCACCTCCTGCAAGAAGAGCTGCTGCTCCTGCTGCCCTGCG
  GAGTGTGAGAAGTGTGCCAAGGACTGTGTGTGCAAAGGCGGAGAGGCAGCTGAGGCAGAA
  GCAGAGAAGTGCAGCTGCTGCCAGTGA
  
  Clone variation with respect to NM_005954.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005954 unedited
  CACGAGGCAGTTGCTTGGAGAAGCCCGTTCACCGCCTCCAGCTGCTGCTCTCCTCGACAT
  GGACCCTGAGACCTGCCCCTGCCCTTCTGGTGGCTCCTGCACCTGCGCGGACTCCTGCAA
  GTGCGAGGGATGCAAATGCACCTCCTGCAAGAAGAGCTGCTGCTCCTGCTGCCCTGCGGA
  GTGTGAGAAGTGTGCCAAGGACTGTGTGTGCAAAGGCGGAGAGGCAGCTGAGGCAGAAGC
  AGAGAAGTGCAGCTGCTGCCAGTGAGAAGGCACCCCTCCGTGTGGAGCACGTGGAGATAG
  TGCCAGGTGGCTCAGTGCCACCTATGCCTGTGGTGAAGTGTGGCTGGTGTCCCCTTCCCC
  TGCTGACCTTGGAGGAATGACAATAAATCCCATGAACAGCANANAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCTCGACTCTAAATTGCGGCCGCGGTCA
  TAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCC
  TGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCA
  TCATTTTGTCTGACTAG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005954 unedited
  ACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTGCCGGTCAAGGGAATTATTGGCATTCCCCCCAGGGCAACAGG
  GGAAGGGGACACCAACCACACTTTACCACAGGCATAAGGGGGACTGAACCACCTGGCACT
  ATTTCCACGTGCTCCAAACGGAGGGGGGCCTTTTTACTGGCAAAAACTGCACTTTTTTGG
  TTTTGCCTCAACTGCCTTTCCCCCTTTGCACACACAATCCTTGGGACACTTTTTACACTT
  CCCAGGGGAACAAGAACAACAACTTTTTTTGCAAGAAGGGCATTTGCATCCCTCCCACTT
  GCAAGAATCCCCCCAAGGGCCGGAACCACCAAAAAGGCAGGGGCAGGGCTCAAGGGCCAT
  GTCCAAGAAAACAACAACTGGAGGCGGGGAAAGGGCTTTTCCAAACAACTGGCTTGTGCC
  CAAATTCCGGGCCCCCTATAAGGAGTCGTATTACAAAAATTTGACGGGTCACTAAAACAA
  CTCTGCTATATAAACCTTCCACCGGACACCCCTACCGGCCATTTGGGTAACGGGGGCCGG
  GGGTATTACCACATTTTGGAAAATCCCCTTGATTTTGGTGCCAAAAAAACTTCTATTGAC
  GTAATGGGGGGGGGAAACTTGGAAATCCCCGTGAGTAAACCGCTTTCACCCCATTGGTGT
  ACTGCCAAACCCCATACCATGGAATAACGATACTAAACCCTANATTACTGGCAGTTAGAA
  AACCCCCTAGGGCATTACTGGCATATGCCCAGCGGCCCTTTACGTATTGACATAAAGGGG
  GGGGCTTGCTATAACCACTGAGACCGCAAGGGGCGTTACCCGAAACTCCCCTTGCCCAAG
  GGAAGCCATTGGCTACTATGAAAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005954
• Insert Size: 470 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005954.2, NP_005945.1
• RefSeq Size: 599 bp
• RefSeq ORF: 207 bp
• Locus ID: 4504
• Cytogenetics: 16q13
• Domains: metalthio
• Gene Summary: 'This gene is a member of the metallothionein family of genes. Proteins encoded by this gene family are low in molecular weight, are cysteine-rich, lack aromatic residues, and bind divalent heavy metal ions. This gene family member displays tissue-specific expression, and contains a threonine insert near its N-terminus and a glutamate-rich hexapeptide insert near its C-terminus relative to the proteins encoded by other gene family members. It plays an important role in zinc and copper homeostasis, and is induced under hypoxic conditions. The encoded protein is a growth inhibitory factor, and reduced levels of the protein are observed in the brains of individuals with some metal-linked neurodegenerative disorders such as Alzheimer's disease. [provided by RefSeq, Sep 2017]'