MTF1 (NM_005955) Human Untagged Clone

CAT#: SC116396

MTF1 (untagged)-Human metal-regulatory transcription factor 1 (MTF1)


  "NM_005955" in other vectors (6)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol MTF1
Synonyms MTF-1; ZRF
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC116396 sequence for NM_005955 edited (data generated by NextGen Sequencing)
ATGGGGGAACACAGTCCAGACAACAACATCATCTACTTTGAGGCAGAGGAAGATGAGCTG
ACCCCCGATGATAAAATGCTCAGGTTTGTGGATAAAAACGGACTGGTGCCTTCCTCATCT
GGAACTGTTTATGATAGGACCACTGTTCTTATTGAGCAGGACCCTGGCACTTTGGAGGAT
GAAGATGACGACGGACAGTGCGGAGAACACTTGCCTTTTCTAGTAGGGGGTGAAGAGGGC
TTTCACCTGATAGATCATGAAGCAATGTCCCAGGGTTATGTGCAGCACATTATCTCACCA
GATCAGATTCATTTGACAATAAACCCTGGTTCCACACCCATGCCAAGAAATATTGAAGGT
GCAACCCTCACTCTGCAGTCGGAATGTCCGGAAACAAAACGTAAAGAAGTAAAGCGGTAC
CAATGTACCTTTGAGGGCTGTCCCCGCACCTACAGCACAGCAGGCAACCTGCGAACCCAC
CAGAAGACTCACCGAGGAGAGTACACCTTTGTCTGTAATCAGGAGGGCTGTGGCAAAGCC
TTCCTTACCTCTTACAGCCTCAGGATCCACGTGCGAGTGCACACGAAGGAGAAGCCATTT
GAGTGTGACGTGCAGGGCTGTGAGAAGGCATTCAACACACTGTACAGGCTGAAAGCACAT
CAGAGGCTTCACACAGGGAAAACGTTTAACTGTGAATCTGAAGGCTGCAGCAAATACTTC
ACCACACTCAGTGATCTGAGGAAGCACATTCGAACTCATACAGGGGAAAAGCCATTTCGG
TGCGATCACGATGGCTGTGGAAAAGCATTTGCAGCAAGCCACCACCTTAAAACTCACGTT
CGTACACATACTGGTGAAAGACCCTTCTTCTGCCCCAGTAATGGCTGTGAGAAAACATTC
AGCACTCAATACAGTCTCAAAAGTCACATGAAAGGTCATGATAACAAAGGACACTCATAC
AATGCACTTCCACAACACAATGGATCAGAGGATACAAATCACTCACTTTGTCTAAGTGAC
TTGAGCCTTCTGTCCACAGATTCTGAATTGCGAGAAAATTCCAGTACGACCCAGGGCCAG
GACCTCAGCACAATTTCACCAGCAATCATCTTTGAATCAATGTTCCAGAATTCAGATGAT
ACGGCAATTCAGGAAGATCCTCAACAGACAGCTTCCTTGACTGAAAGTTTTAATGGTGAT
GCAGAGTCAGTCAGTGATGTTCCGCCATCCACAGGAAATTCAGCATCTTTATCTCTTCCA
CTTGTACTGCAACCTGGCCTCTCCGAGCCACCCCAGCCTCTACTACCTGCCTCAGCTCCG
TCTGCTCCTCCGCCTGCTCCCTCCCTAGGACCTGGCTCCCAGCAAGCTGCATTTGGCAAC
CCCCCTGCTCTCTTACAACCTCCAGAAGTGCCTGTTCCCCACAGCACACAGTTTGCTGCT
AATCATCAAGAGTTTCTTCCGCACCCCCAGGCACCGCAGCCCATTGTACCAGGACTTTCT
GTTGTTGCTGGGGCTTCTGCATCAGCAGCGGCAGTGGCATCAGCTGTGGCAGCACCAGCC
CCACCACAAAGTACTACTGAGCCCCTGCCAGCCATGGTCCAGACTCTGCCCCTGGGTGCC
AACTCTGTCCTAACTAATAATCCCACAATAACCATCACCCCAACTCCCAACACAGCTATC
CTGCAGTCCAGCCTAGTCATGGGAGAACAGAACTTACAATGGATATTAAATGGTGCCACC
AGTTCTCCACAAAACCAAGAACAAATTCAGCAAGCATCTAAAGTTGAGAAGGTGTTTTTT
ACCACTGCAGTACCAGTAGCCAGTAGCCCAGGGAGCTCTGTCCAGCAGATTGGCCTCAGT
GTTCCTGTGATCATCATCAAACAAGAAGAGGCATGTCAGTGTCAGTGTGCATGCCGGGAC
TCTGCAAAGGAGCGGGCATCCAGCAGGAGAAAGGGCTGCTCCTCCCCACCCCCTCCAGAG
CCGAGCCCCCAGGCTCCTGATGGGCCCAGCCTGCAGCTCCCAGCGCAGACTTTCTCTTCA
GCCCCTGTTCCCGGGTCATCATCCTCTACCTTGCCCTCCTCCTGTGAGCAAAGCCGACAA
GCAGAGACTCCTTCAGACCCTCAGACAGAAACATTAAGTGCCATGGATGTGTCAGAGTTT
CTATCCCTCCAGAGCCTGGACACCCCGTCCAATCTGATTCCCATTGAAGCACTACTGCAG
GGGGAGGAGGAGATGGGCCTCACCAGCAGCTTCTCCAAGTGA

Clone variation with respect to NM_005955.2
>OriGene 5' read for NM_005955 unedited
ATTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCTGGGGAGGGGAGAA
GCTGCTGCTGCCGCCGTTGCCGGGAGCCGCGGAGACAAGTCATTACGTTTTCATTTCTCA
CAACTGGGCTGAGCACAACTGAACCATGGGGGAACACAGTCCAGACAACAACATCATCTA
CTTTGAGGCAGAGGAAGATGAGCTGACCCCCGATGATAAAATGCTCAGGTTTGTGGATAA
AAACGGACTGGTGCCTTCCTCATCTGGAACTGTTTATGATAGGACCACTGTTCTTATTGA
GCAGGACCCTGGCACTTTGGAGGATGAAGATGACGACGGACAGTGCGGAGAACACTTGCC
TTTTCTAGTAGGGGGTGAAGAGGGCTTTCACCTGATAGATCATGAAGCAATGTCCCAGGG
TTATGTGCAGCACATTATCTCACCAGATCAGATTCATTTGACAATAAACCCTGGTTCCAC
ACCCATGCCAAGAAATATTGAAGGTGCAACCCTCACTCTGCAGTCGGAATGTCCGGAAAC
AAAACGTAAAGAAGTAAAGCGGTACCAATGTACCTTTGAGGGCTGTCCCCGCACCTACAG
CACAGCAGGCAACCTGCGAACCCACCAGAAGACTCACCGAGGAGAGTACACCTTTGTCTG
TAATCAGGAGGGCTGTGGCAAAGCCTTCCTTACCTCTTACAGCCTCANGATCCACGTGCG
AGTGCACACGANAGAGAAGCCATTTGAGTGTGACGTGCAGGGCTGTTGAGAGGCATTCAA
CACACTGTACAGGCTGAAAGCACATCAGAGGCTTCACACAGGGAAAAACGTTACTGTGAA
TCTGAAGGCTGCAGCAAATACTTCACCACACTCAGTGATCTGTAGAAGCACATTCGACCT
CATACAGGGGAAAAGCCATTTTCGTGCGATCACGATGGCTGTGGNAAAGCATTTGCAGCA
AGCCCACACCTTAAAACTCACGNTCGTACCN
>OriGene 3' read for NM_005955 unedited
CGGCCGCAATCTAGAGTCGAGNNNTTNTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT
TGGTTTTTTTTTTGGTTTTTTTTTTTTGGTTTTTTTCAAAAAATAATTCCTTAAAAAGGA
TGCTCCTGGGATGGGAATAAAAAAAATACCTCTGAAAGGGGAGGATTTCAAACAGTAAAA
TTCTTTATCCTTTAAAATTCTGACCCATGATGGGAGGCATTGGACCTCATGCCTCCTGGT
CACCCGCTTTTTCCAAAAGGGAACACACATGGGCCCTTTACTTGGAAAAACTGGTGGTGA
GGGCCATTTTCTTCTTCCCCTGGAGTAGTGCTTCAATGGGAATCAAATTGGACCGGGTGT
CCAAGCTCTGGAGGGATAAAAACTCTGACACATCCCTGGCACTTAATGGTTCTGTCTGAA
GGTCTGAAAGAATCTCTGCTTGTCGGCTTTTCTCACAAGAGGAGGGCAAGGTAAAAGATG
ATGACCCCGGAACAGGGGCTGAAGAAAAAATCTGCGCTGGGAACTGCAAGCTGGGCCCAT
CAAGAACCTGGGGGCTCGGCTCTTGAGGGGGTGGGGAGGAACAACCCTTTCTTCTGCTGG
ATGCCCGCTCCTTTGCAGAGTCCCGGCATGCACACTGACACTGACATGCCTCTTCTTGTT
TGATGATGATCACAAGAACACTGAAGCCACATCTGCTGGACAGAACTCCCTGGGCTACTG
GCTACTGGTACTGCAGNGGGAAAAACACCTTCTCAACTTTAGAAGCCTGCTGAAATTNGT
NCTTGGGTTTGTGGAGAACTGGTGGCACCATTTAATATCACTTGGAAGTTCTGGTCTCCA
TGACANNNGCTGACTGCAGATAACTTGNTGGGAGTTGGGGTGATTGTTATTGGGGGAATA
TATAANTAGGCACGNAGTTGGCACCAGGGNCACAATCTGGACCATGGCCTGCAGGGCTCA
TANAACTTTTGGNGNGGGGGGCTTGGGCTGCCCACCTGA
Restriction Sites NotI-NotI     
ACCN NM_005955
Insert Size 2980 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005955.1, NP_005946.1
RefSeq Size 3302 bp
RefSeq ORF 2262 bp
Locus ID 4520
Cytogenetics 1p34.3
Domains zf-C2H2
Protein Families Transcription Factors
Gene Summary 'This gene encodes a transcription factor that induces expression of metallothioneins and other genes involved in metal homeostasis in response to heavy metals such as cadmium, zinc, copper, and silver. The protein is a nucleocytoplasmic shuttling protein that accumulates in the nucleus upon heavy metal exposure and binds to promoters containing a metal-responsive element (MRE). [provided by RefSeq, Jul 2008]'

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