RNF6 (NM_005977) Human Untagged Clone

CAT#: SC116405

RNF6 (untagged)-Human ring finger protein (C3H2C3 type) 6 (RNF6), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RNF6
• Synonyms: DKFZp686P0776
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005977 edited
  GAATTCGGCACGAGGCGAGGGCACAGCGGGCTCCGGAGGCGCGGCGGCAAGCCTATCCCG
  CCTCCCAACCACAGCCTCCAGCACCCGAGAGAACGGCCGCCCACAGCACACGTTCTCCGG
  ACAGGAGGGCGAAGGCCCAAGACCTGGAGAGGTGGGCTCGAGGGAAAGATCTGGGTCCCA
  CCACTACTTCCTGGAGACGCCGGGGAAAGCAGGCCATCCTTCGAGCCACCCATCTCTTCT
  CCGATTGGTTAACTGCCTTCTTATTGCGCTCCGCTGGCTGGTGATTGGTTGGGATGCACC
  GTCTATCACCTGCTTCCTTGTGAGAATCTAGGTCTCTAAGGGAAGCGTTACTTGAGGCTC
  GGTTGGGAAGAGATGGGCAGCTCTCAAAAAAGGCACAAACAATTGAAGGATGGATACCAT
  GGCATATGTTAAAAGCGTGTTGAAAGGAAAATAAGAAAGCCAGGAATCTCAGGATGAATC
  AGTCTAGATCGAGATCAGATGGTGGCAGTGAAGAAACCTTACCTCAAGACCATAATCATC
  ATGAAAATGAGAGAAGATGGCAGCAAGAGCGTCTCCACAGAGAAGAGGCCTATTATCAGT
  TTATTAATGAACTCAATGATGAAGATTATCGGCTTATGAGAGACCATAATCTTTTAGGCA
  CCCCTGGAGAAATAACATCAGAAGAACTGCAACAGCGGTTAGATGGCGTCAAGGAACAAC
  TAGCATCTCAGCCTGACTTGAGAGATGGAACGAATTACAGAGACTCAGAAGTCCCTAGAG
  AAAGTTCACATGAAGATTCTCTTCTAGAATGGTTGAACACCTTTCGGCGCACAGGAAATG
  CAACTCGAAGTGGACAAAATGGGAACCAAACTTGGAGAGCTGTGAGTCGAACAAACCCGA
  ACAATGGAGAGTTTCGGTTTAGTTTGGAAATCCACGTAAATCATGAAAATAGAGGATTTG
  AAATTCATGGAGAAGATTATACAGACATTCCACTTTCAGATAGTAACAGAGATCATACTG
  CAAATAGGCAACAAAGGTCAACTAGTCCTGTGGCTAGGCGAACAAGAAGCCAAACCTCAG
  TGAATTTCAATGGTAGTAGTTCCAACATTCCAAGGACTAGGCTTGCTTCAAGGGGGCAAA
  ATCCAGCTGAAGGATCTTTCTCAACATTGGGAAGGTTAAGAAATGGAATTGGGGGAGCAG
  CTGGCATTCCTCGAGCTAACGCTTCACGCACTAATTTCAGTAGTCACACAAACCAATCAG
  GTGGTAGTGAACTCAGGCAAAGGGAGGGGCAACGGTTTGGAGCAGCACATGTTTGGGAAA
  ATGGGGCTAGAAGTAATGTTACAGTGAGGAATACAAACCAAAGATTAGAGCCAATAAGAT
  TACGATCTACTTCCAATAGTCGAAGCCGTTCACCAATTCAGAGACAGAGTGGCACTGTTT
  ATCATAATTCCCAAAGGGAAAGTAGACCAGTACAGCAAACCACTAGAAGATCTGTTAGGA
  GGAGAGGTAGAACTCGAGTCTTTTTAGAGCAAGATAGAGAACGAGAACGCAGAGGTACTG
  CATATACCCCATTCTCTAATTCAAGGCTTGTGTCAAGAATAACAGTAGAAGAAGGAGAAG
  AATCCAGCAGATCCTCAACTGCTGTACGACGACATCCAACAATCACACTGGACCTTCAAG
  TGAGAAGGATCCGTCCTGGAGAAAATAGAGATCGGGATAGTATTGCAAATAGAACTCGAT
  CCAGAGTAGGGCTAGCAGAAAATACAGTCACTATTGAAAGCAATAGTGGGGGCTTTCGCC
  GAACCATTTCTCGTTTAGAGCGGTCAGGTATTCGAACCTATGTTAGTACCATAACAGTTC
  CTCTTCGTAGGATTTCTGAGAATGAGCTTGTTGAGCCATCATCAGTGGCTCTTCGGTCAA
  TTTTAAGGCAGATCATGACTGGGTTTGGAGAACTGAGTTCTCTAATGGAGGCCGATTCTG
  AGTCAGAACTTCAAAGAAATGGCCAGCATTTACCAGACATGCACTCAGAACTGAGTAACT
  TAGGTACAGATAACAACAGGAGCCAGCACAGGGAAGGTTCCTCTCAAGACAGGCAGGCCC
  AAGGAGACAGCACTGAAATGCATGGTGAAAACGAGACCACCCAGCCTCATACTCGAAACA
  GTGACAGTAGGGGTGGCAGGCAGTTGCGAAATCCAAACAATTTAGTTGAAACTGGAACAC
  TACCCATTCTTCGCCTTGCTCACTTTTTTTTACTAAATGAAAGTGATGATGATGATCGAA
  TACGTGGTTTAACCAAAGAGCAGATTGACAATCTTTCCACCAGGCACTATGAGCATAACA
  GTATTGATAGTGAACTAGGTAAAATCTGTAGTGTTTGTATTAGTGACTATGTAACTGGAA
  ACAAGCTCAGGCAATTACCTTGCATGCATGAATTTCACATTCATTGTATTGACCGATGGC
  TCTCAGAGAATTGCACTTGTCCGATCTGTCGGCAGCCTGTTTTAGGGTCTAACATAGCAA
  ACAATGGGTAAGGTGATGGGATCTACTCAAATACTGTTTTTTAGTAGAACTGAATGTTCA
  AGCATTGTTTTGCTGAGTTATTTGTGATTAGCTAACCAGGATGAAAAATAACAGATTATA
  TATAGTTTGAACTATTTTTCGTGTGCTTTTTTAAACTTGTTAAAAAGAAATTTATATAAA
  ATTTAAAATACAAATGTTAAATTATCCAGAAATACAGAATAGTTAATATTGCTAGAACCA
  AATAACCTCTAAAATGTTTTTATTTTGGTAATTTTGTCATGCTAAGCACTTTTGTATCTG
  CACAATTCAGTAGGTTAAGAATCAATCTTCTTTTTCTTAATAGTACAGCAGACTTTAGCT
  TCAAGTTTCATAGGCTTAGTACTTATATCTAGACATTTGTGTCTAAATAAGCTTTTCATT
  AACTTTTTATTTTAAGGACAGTATCTTTTCATGAAAGAGTATTTGGCTGAATGTTTGCTA
  TATATATGTTACTTGAAATGTTAAATTTAATATGCAGCATACCATAGGTGTATATATAGG
  TATATAATTTTAAGGTTAAAATATTCAGTCTACAAGTTTGGTTCTTATTTAAGCTTTTGG
  GCTAATACTGCATATGGCACAATGTTTAATATTGGCAAGTTCATCTCAGAGAAAGGGGAT
  TCAGATATAATTTTAAAGTAGAGATAATTTACTGAAGCGTCTCTGACAATCTAACTTATT
  AGACAGCAAGCAATATATAATACTGAAAAAGTATTCAGAAATGGAAAATTTACATCATAT
  AGGTTATTTAACTTGTGTTCAGCCTTTTTGTAACTTTTTTGAAAGTGCAAACAATTCTTT
  GGATTATTAAATAAGGTATACAGTATGCATGGTTTCTCAAATTTAGTTTTAAAATCTAAA
  AGTCTATAAAGAATCAGATGCATAGGCAATATGTTAAGTTCACTTGGAGGCTAAAAATCT
  CCAGTGAAAACAAAACGAAAACCTTTAAGAGAATGTAGAGTTTATATAAACACAAAGTAT
  GCATTGAAGATCTGTTTCTACCAATAAACATTAAAACAAAAAAAAAAAAAAAAAACTCGA
  C
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005977 unedited
  GGGTACANAATATGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGCGAGGGC
  ACAGCGGGCTCCCGGAGCGCGGCGGCAAGCCTATCCCGCCTCCCAACCACAGCCTCCAGC
  ACCCGAGAGAACGGCCGCCCACAGCACACGTTCTCCGGACAGGAGGGCGAAGGCCCAAGA
  CCTGGAGAGGTGGGCTCGAGGGAAAGATCTGGGTCCCACCACTACTTCCTGGAGACGCCG
  GGGAAAGCAGGCCATCCTTCGAGCCACCCATCTCTTCTCCGATTGGTTAACTGCCTTCTT
  ATTGCGCTCCGCTGGCTGGTGATTGGTTGGGATGCACCGTCTATCACCTGCTTCCTTGTG
  AGAATCTAGGTCTCTAAGGGAAGCGTTACTTGAGGCTCGGTTGGGAAGAGATGGGCAGCT
  CTCAAAAAAGGCACAAACAATTGAAGGATGGATACCATGGCATATGTTAAAAGCGTGTTG
  AAAGGAAAATAAGAAAGCCAGGAATCTCAGGATGAATCAGTCTAGATCGAGATCAGATGG
  TGGCAGTGAAGACACCTTACCTCAAGACCATAATCATCATGAAAATGAGAGAAGATGGCA
  GCAAGAGCGTCTCCACAGAGAAGAGGCCTATTATCAGTTTATTAATGAACTCAATGATGA
  AGATTATCCGCCTTATGAGAGACCATAATCTTTTTAGCACCCCTGGAGAATAACATCAGA
  AGAACTGGCACAGCGGTTAGATGGCGTCAGGAACAACTAGCATCTCAGCCTGACTTGAGA
  GATGGAACCGATTACCGAGACTCAGCAGTCCCTAGAAAAAGTCACATGAAGATTCTCTTC
  TAGATGGGTGAACCCCTTTCGCGGCCCAGGAAAGCCACCTGAAGTGGACAAATGGGAACC
  AACTTGGAAGCTGTGAGCCAN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005977 unedited
  CGCTTGCCCGGGCCGCTTCTATGATCGGTTTTTTTTTTTTTTTTTTGGTTTAATGTTTAT
  TGGTAGAAACAGATCTTCAATGCATACTTTGTGTTTATATAAACTCTACATTCTCTTAAA
  GGTTTTCGTTTTGTTTTCACTGGAGATTTTTAGCCTCCAAGTGAACTTAACATATTGCCT
  ATGCATCTGATTCTTTATAGACTTTTAGATTTTAAAACTAAATTTGAGAAACCATGCATA
  CTGTATACCTTATTTAATAATCCAAAGAATTGTTTGCACTTTCAAAAAAGTTACAAAAAG
  GCTGAACACAAGTTAAATAACCTATATGATGTAAATTTTCCATTTCTGAATACTTTTTCA
  GTATTATATATTGCTTGCTGTCTAATAAGTTAGATTGTCAGAGACGCTTCAGTAAATTAT
  CTCTACTTTAAAATTATATCTGAATCCCCTTTCTCTGAGATGAACTTGCCAATATTAAAC
  ATTGTGCCATATGCAGTATTAGCCCAAAAGCTTAAATAAGAACCAAACTTGTAGACTGAA
  TATTTTAACCTTAAAATTATATACCTATATATACACCTATGGTATGCTGCATATTAAATT
  TAACATTTCAAGTTACATATTTATAGCAAACATTCAGCCAAATACTCTTTCATGAAAAGA
  TACTGTCCTTAAAATAAAAAGGTAATGAAAAGCTTATTTAGACACAAATGCCTAGATATA
  AGTACTAAGCCTATGAAACTCGAACCTAAAGTCTGCCGTTCTATTAAGAAAAAGAAGATT
  GATCCTCAACCTACTGAAATGTGCAAATCCAAAGTGCCTAACCCTGCAAAATTCACCAAA
  ATTAAACCTTCTTAAAGGNTATTATGGTCCTAACAAATTAAACTTCCCTGCTCTTCCGGA
  TAATTAAACTTCTGCTTTTACATCTTATATAATTCCCCTTTCACAACNCCAAAAAGCCAC
  GCAAACTGCCCAACCTCTCTATCCTGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005977
• Insert Size: 3720 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005977.2, NP_005968.1
• RefSeq Size: 3598 bp
• RefSeq ORF: 2058 bp
• Locus ID: 6049
• Cytogenetics: 13q12.13
• Domains: RING
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene contains a RING-H2 finger motif. Deletions and mutations in this gene were detected in esophageal squamous cell carcinoma (ESCC), suggesting that this protein may be a potential tumor suppressor. Studies of the mouse counterpart suggested a role of this protein in the transcription regulation that controls germinal differentiation. Multiple alternatively spliced transcript variants encoding the same protein are observed. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1, 3, and 4 all encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.