RNF6 (NM_005977) Human Untagged Clone

CAT#: SC324174

RNF6 (untagged)-Human ring finger protein (C3H2C3 type) 6 (RNF6), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RNF6
• Synonyms: DKFZp686P0776
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005977.2 TTGGGAAGAGATGGTCAGCTCTCAAAAAAGGCACAAACAATTGAAGGATGGATACCATGG
  CATATGTTAAAAGCGTGTTGAAAGGAAAATAAGAAAGCCAGGAATCTCAGGATGAATCAG
  TCTAGATCGAGATCAGATGGTGGCAGTGAAGAAACCTTACCTCAAGACCATAATCATCAT
  GAAAATGAGAGAAGATGGCAGCAAGAGCGTCTCCACAGAGAAGAGGCCTATTATCAGTTT
  ATTAATGAACTCAATGATGAAGATTATCGGCTTATGAGAGACCATAATCTTTTAGGCACC
  CCTGGAGAAATAACATCAGAAGAACTGCAACAGCGGTTAGATGGCGTCAAGGAACAACTA
  GCATCTCAGCCTGACTTGAGAGATGGAACGAATTACAGAGACTCAGAAGTCCCTAGAGAA
  AGTTCACATGAAGATTCTCTTCTAGAATGGTTGAACACCTTTCGGCGCACAGGAAATGCA
  ACTCGAAGTGGACAAAATGGGAACCAAACTTGGAGAGCTGTGAGTCGAACAAACCCGAAC
  AATGGAGAGTTTCGGTTTAGTTTGGAAATCCACGTAAATCATGAAAATAGAGGATTTGAA
  ATTCATGGAGAAGATTATACAGACATTCCACTTTCAGATAGTAACAGAGATCATACTGCA
  AATAGGCAACAAAGGTCAACTAGTCCTGTGGCTAGGCGAACAAGAAGCCAAACCTCAGTG
  AATTTCAATGGTAGTAGTTCCAACATTCCAAGGACTAGGCTTGCTTCAAGGGGGCAAAAT
  CCAGCTGAAGGATCTTTCTCAACATTGGGAAGGTTAAGAAATGGAATTGGGGGAGCAGCT
  GGCATTCCTCGAGCTAACGCTTCACGCACTAATTTCAGTAGTCACACAAACCAATCAGGT
  GGTAGTGAACTCAGGCAAAGGGAGGGGCAACGGTTTGGAGCAGCACATGTTTGGGAAAAT
  GGGGCTAGAAGTAATGTTACAGTGAGGAATACAAACCAAAGATTAGAGCCAATAAGATTA
  CGATCTACTTCCAATAGTCGAAGCCGTTCACCAATTCAGAGACAGAGTGGCACTGTTTAT
  CATAATTCCCAAAGGGAAAGTAGACCAGTACAGCAAACCACTAGAAGATCTGTTAGGAGG
  AGAGGTAGAACTCGAGTCTTTTTAGAGCAAGATAGAGAACGAGAACGCAGAGGTACTGCA
  TATACCCCATTCTCTAATTCAAGGCTTGTGTCAAGAATAACAGTAGAAGAAGGAGAAGAA
  TCCAGCAGATCCTCAACTGCTGTACGACGACATCCAACAATCACACTGGACCTTCAAGTG
  AGAAGGATCCGTCCTGGAGAAAATAGAGATCGGGATAGTATTGCAAATAGAACTCGATCC
  AGAGTAGGGCTAGCAGAAAATACAGTCACTATTGAAAGCAATAGTGGGGGCTTTCGCCGA
  ACCATTTCTCGTTTAGAGCGGTCAGGTATTCGAACCTATGTTAGTACCATAACAGTTCCT
  CTTCGTAGGATTTCTGAGAATGAGCTTGTTGAGCCATCATCAGTGGCTCTTCGGTCAATT
  TTAAGGCAGATCATGACTGGGTTTGGAGAACTGAGTTCTCTAATGGAGGCCGATTCTGAG
  TCAGAACTTCAAAGAAATGGCCAGCATTTACCAGACATGCACTCAGAACTGAGTAACTTA
  GGTACAGATAACAACAGGAGCCAGCACAGGGAAGGTTCCTCTCAAGACAGGCAGGCCCAA
  GGAGACAGCACTGAAATGCATGGTGAAAACGAGACCACCCAGCCTCATACTCGAAACAGT
  GACAGTAGGGGTGGCAGGCAGTTGCGAAATCCAAACAATTTAGTTGAAACTGGAACACTA
  CCCATTCTTCGCCTTGCTCACTTTTTTTTACTAAATGAAAGTGATGATGATGATCGAATA
  CGTGGTTTAACCAAAGAGCAGATTGACAATCTTTCCACCAGGCACTATGAGCATAACAGT
  ATTGATAGTGAACTAGGTAAAATCTGTAGTGTTTGTATTAGTGACTATGTAACTGGAAAC
  AAGCTCAGGCAATTACCTTGCATGCATGAATTTCACATTCATTGTATTGACCGATGGCTC
  TCAGAGAATTGCACTTGTCCGATCTGTCGGCAGCCTGTTTTAGGGTCTAACATAGCAAAC
  AATGGGTAAGGTGATGGGATCTACTCAAATACTGTTTTTTAGTAGAACTGAATGTTCAAG
  CATTGTTTTGCTGAGTTATTTGTGATTAGCTAACCAGGATGAAAAATAACAGATTATATA
  TAGTTTGAACTATTTTTCGTGTGCTTTTTTAAACTTGTTAAAAAGAAATTTATATAAAAT
  TTAAAATACAAATGTTAAATTATCCAGAAATACAGAATAGTTAATATTGCTAGAACCAAA
  TAACCTCTAAAATGTTTTTATTTTGGTAATTTTGTCATGCTAAGCACTTTTGTATCTGCA
  CAATTCAGTAGGTTAAGAATCAATCTTCTTTTTCTTAATAGTACAGCAGACTTTAGCTTC
  AAGTTTCATAGGCTTAGTACTTATATCTAGACATTTGTGTCTAAATAAGCTTTTCATTAA
  CTTTTTATTTTAAGGACAGTATCTTTTCATGAAAGAGTATTTGGCTGAATGTTTGCTATA
  TATATGTTACTTGAAATGTTAAATTTAATATGCAGCATACCATAGGTGTATATATAGGTA
  TATAATTTTAAGGTTAAAATATTCAGTCTACAAGTTTGGTTCTTATTTAAGCTTTTGGGC
  TAATACTGCATATGGCACAATGTTTAATATTGGCAAGTTCATCTCAGAGAAAGGGGATTC
  AGATATAATTTTAAAGTAGAGATAATTTACTGAAGCGTCTCTGACAATCTAACTTATTAG
  ACAGCAAGCAATATATAATACTGAAAAAGTATTCAGAAATGGAAAATTTACATCATATAG
  GTTATTTAACTTGTGTTCAGCCTTTTTGTAACTTTTTTGAAAGTGCAAACAATTCTTTGG
  ATTATTAAATAAGGTATCCAGTATGCATGGTTTCTCAAATTTAGCTTTAAAATCTAAAAG
  TCTATAAAGAATCAGATGCATAGGCAATATGTTAAGTTCACTTGGAGGCTAAAAATCTCC
  AGTGAAAACAAAACGAAAACCTTTAAGAGAATGTAGAGTTTATATAAACACAAAGTATGC
  ATTGAAGATCTGTTTCTACCAATAAACATTAAACCAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_005977
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005977.2, NP_005968.1
• RefSeq Size: 3598 bp
• RefSeq ORF: 2058 bp
• Locus ID: 6049
• Cytogenetics: 13q12.13
• Domains: RING
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene contains a RING-H2 finger motif. Deletions and mutations in this gene were detected in esophageal squamous cell carcinoma (ESCC), suggesting that this protein may be a potential tumor suppressor. Studies of the mouse counterpart suggested a role of this protein in the transcription regulation that controls germinal differentiation. Multiple alternatively spliced transcript variants encoding the same protein are observed. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1, 3, and 4 all encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.