TBX3 (NM_005996) Human Untagged Clone

CAT#: SC116413

TBX3 (untagged)-Human T-box 3 (TBX3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TBX3
• Synonyms: TBX3-ISO; UMS; XHL
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005996, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCCTCTCCATGAGAGATCCGGTCATTCCTGGGACAAGCATGGCCTACCATCCGTTCCTACCTCACC
  GGGCGCCGGACTTCGCCATGAGCGCGGTGCTGGGTCACCAGCCGCCGTTCTTCCCCGCGCTGACGCTGCC
  TCCCAACGGCGCGGCGGCGCTCTCGCTGCCGGGCGCCCTGGCCAAGCCGATCATGGATCAATTGGTGGGG
  GCGGCCGAGACCGGCATCCCGTTCTCCTCCCTGGGGCCCCAGGCGCATCTGAGGCCTTTGAAGACCATGG
  AGCCCGAAGAAGAGGTGGAGGACGACCCCAAGGTGCACCTGGAGGCTAAAGAACTTTGGGATCAGTTTCA
  CAAGCGGGGCACCGAGATGGTCATTACCAAGTCGGGAAGGCGAATGTTTCCTCCATTTAAAGTGAGATGT
  TCTGGGCTGGATAAAAAAGCCAAATACATTTTATTGATGGACATTATAGCTGCTGATGACTGTCGTTATA
  AATTTCACAATTCTCGGTGGATGGTGGCTGGTAAGGCCGACCCCGAAATGCCAAAGAGGATGTACATTCA
  CCCGGACAGCCCCGCTACTGGGGAACAGTGGATGTCCAAAGTCGTCACTTTCCACAAACTGAAACTCACC
  AACAACATTTCAGACAAACATGGATTTACTATATTGAACTCCATGCACAAATACCAGCCCCGGTTCCACA
  TTGTAAGAGCCAATGACATCTTGAAACTCCCTTATAGTACATTTCGGACATACTTGTTCCCCGAAACTGA
  ATTCATCGCTGTGACTGCATACCAGAATGATAAGATAACCCAGTTAAAAATAGACAACAACCCTTTTGCA
  AAAGGTTTCCGGGACACTGGAAATGGCCGAAGAGAAAAAAGAAAACAGCTCACCCTGCAGTCCATGAGGG
  TGTTTGATGAAAGACACAAAAAGGAGAATGGGACCTCTGATGAGTCCTCCAGTGAACAAGCAGCTTTCAA
  CTGCTTCGCCCAGGCTTCTTCTCCAGCCGCCTCCACTGTAGGGACATCGAACCTCAAAGATTTATGTCCC
  AGCGAGGGTGAGAGCGACGCCGAGGCCGAGAGCAAAGAGGAGCATGGCCCCGAGGCCTGCGACGCGGCCA
  AGATCTCCACCACCACGTCGGAGGAGCCCTGCCGTGACAAGGGCAGCCCCGCGGTCAAGGCTCACCTTTT
  CGCTGCTGAGCGGCCCCGGGACAGCGGGCGGCTGGACAAAGCGTCGCCCGACTCACGCCATAGCCCCGCC
  ACCATCTCGTCCAGCACTCGCGGCCTGGGCGCGGAGGAGCGCAGGAGCCCGGTTCGCGAGGGCACAGCGC
  CGGCCAAGGTGGAAGAGGCGCGCGCGCTCCCGGGCAAGGAGGCCTTCGCGCCGCTCACGGTGCAGACGG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005996 unedited
  NGGTTCAGGTTTTGTATCCGATTTACTATAGGCGGCACGCCAATTCGCACGGNAAGACAC
  TTCCCCTCCTCCCTTTGAAGTGCATTAGTTGTGATTTCTGCCTCCTTTTCTTTTTTCTTT
  CTTTTTTGTTTTGCTTTTTCCCCCCTTTTGAATTATGTGCTGCTGTTAAACAACAACAAA
  AAAACAACAAAACACAGCAGCTGCGGACTTGTCCCCTGTTGGAGCCCAGCGCCCCGCCTG
  GAGTGGATGAGCCTCTCCATGAGAGATCCGGTCATTCCTGGGACAAGCATGGCCTACCAT
  CCGTTCCTACCTCACCGGGCGCCGGACTTCGCCATGAGCGCGGTGCTGGGTCACCAGCCG
  CCGTTCTTCCCCGCGCTGACGCTGCCTCCCAACGGCGCGGCGGCGCTCTCGCTGCCGGGC
  GCCCTGGCCAAGCCGATCATGGATCAATTGGTGGGGGCGGCCGAGACCGGCATCCCGTTC
  TCCTCCCTGGGGCCCCAGGCGCATCTGAGGCCTTTGAAGACCATGGAGCCCGAAGAAGAG
  GTGGAGGACGACCCCAAGGTGCACCTGGAGGCTAAAGAACTTTGGGATCAGTTTCACAAG
  CGGGGCACCGAGATGGTCATTACCAAGTCGGGAAGGCGAATGTTTCCTCCATTTAAAGTG
  AGATGTTCTGGGCTGGATAAAAAAGCCAAATACATTTTATTGATGGACATTATAGCTGCT
  GATGACTGTCGTTATAAATTTCACAAATTCTCGTGGATGGTGGCTGGTAAGGCCGACCCC
  GAATGCCAAAGAGATGTACATTCACCCGACAGCCCCGCTACTGGGGAACAGTGGATGTCC
  AAAGTCGTCACTTTCCACAAACTGAAACTCACCCACACATTTCAGACCAACATGGATTTA
  CTTTTGGCTTTCCCAGTGATCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005996 unedited
  GTTAAATTCCTTTTTTTTTTGTTGGTGTTTCCACTTGGCACAGTGAAGGAAAAATTATAT
  ATAAATCCGCACTGAGGGAGATGTCTTTGAACACCTCCCCGCCTGGTGGGCAGAGACCCA
  GACCAGCCTTGCTGGAAGTTGCTCTGGACATAAATGTTGGAACTCCTACCCCCAGTAGCT
  CAATGCAACCGACGTTTCTGAGCCCCCAGAATCTGATCCAGATCCCGGACATATAAACCA
  CGCCAAGAAGACAGGGGCTGTCTCTAGCACATTCTCTCGAGGGAGTCCGGGGCCCCTTCC
  CAGACGCAACTGCAAAAGGAAGGGCTAACGCCATGGCGGGCCCGTGGTTTATTTTATATC
  CGACAAAGTGCACGGCAGCCTGAACTGGACTGGAATGAAAAGACGTGTCTGGGACGGGTC
  TACGGGGACGCGCTGCGGGACCTGTCCGGCTTGGCTTCCAAGCCGCTAACCAACCGCTGG
  ATGCTCTGCAGTTCGCTGGTGGCCGCCTCTTTCTCCGCGCAGAGTTTGGGCGACAAGGAC
  ATGGAGCTGGAGGATAGCGTGGAGGATCGGCTGTTGAGTTCATAGCCCGAGTCCACTGCC
  ACCGATGCCGGGCTGGCGGCCAGGGCGGCGACTTTGCCGTCCAGGGGCCCCGCGGCCGCC
  GCCATGGATGGCATGTCGGTGGTGAGCATACTGCTGCCGTCCGGGACCGGCACCGGGATG
  GNAGTAGGGCTGTAGCGCANCCGCGGGCGCTGGTGTTCANATTGAGAAGGNGTGGGCGTG
  CACCGAGCTGGAGCTGNCGCANAGGAGGCGGCCGCCCTGCGCCATGTACGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005996
• Insert Size: 2700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005996.3, NP_005987.3
• RefSeq Size: 4754 bp
• RefSeq ORF: 2172 bp
• Locus ID: 6926
• Cytogenetics: 12q24.21
• Domains: T-box
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This protein is a transcriptional repressor and is thought to play a role in the anterior/posterior axis of the tetrapod forelimb. Mutations in this gene cause ulnar-mammary syndrome, affecting limb, apocrine gland, tooth, hair, and genital development. Alternative splicing of this gene results in three transcript variants encoding different isoforms; however, the full length nature of one variant has not been determined. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) encodes the shorter isoform (1) of this protein.