SMAD2 (NM_005901) Human Untagged Clone

CAT#: SC116434

SMAD2 (untagged)-Human SMAD family member 2 (SMAD2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SMAD2
• Synonyms: hMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116434 sequence for NM_005901 edited (data generated by NextGen Sequencing)
  ATGTCGTCCATCTTGCCATTCACGCCGCCAGTTGTGAAGAGACTGCTGGGATGGAAGAAG
  TCAGCTGGTGGGTCTGGAGGAGCAGGCGGAGGAGAGCAGAATGGGCAGGAAGAAAAGTGG
  TGTGAGAAAGCAGTGAAAAGTCTGGTGAAGAAGCTAAAGAAAACAGGACGATTAGATGAG
  CTTGAGAAAGCCATCACCACTCAAAACTGTAATACTAAATGTGTTACCATACCAAGCACT
  TGCTCTGAAATTTGGGGACTGAGTACACCAAATACGATAGATCAGTGGGATACAACAGGC
  CTTTACAGCTTCTCTGAACAAACCAGGTCTCTTGATGGTCGTCTCCAGGTATCCCATCGA
  AAAGGATTGCCACATGTTATATATTGCCGATTATGGCGCTGGCCTGATCTTCACAGTCAT
  CATGAACTCAAGGCAATTGAAAACTGCGAATATGCTTTTAATCTTAAAAAGGATGAAGTA
  TGTGTAAACCCTTACCACTATCAGAGAGTTGAGACACCAGTTTTGCCTCCAGTATTAGTG
  CCCCGACACACCGAGATCCTAACAGAACTTCCGCCTCTGGATGACTATACTCACTCCATT
  CCAGAAAACACTAACTTCCCAGCAGGAATTGAGCCACAGAGTAATTATATTCCAGAAACG
  CCACCTCCTGGATATATCAGTGAAGATGGAGAAACAAGTGACCAACAGTTGAATCAAAGT
  ATGGACACAGGCTCTCCAGCAGAACTATCTCCTACTACTCTTTCCCCTGTTAATCATAGC
  TTGGATTTACAGCCAGTTACTTACTCAGAACCTGCATTTTGGTGTTCGATAGCATATTAT
  GAATTAAATCAGAGGGTTGGAGAAACCTTCCATGCATCACAGCCCTCACTCACTGTAGAT
  GGCTTTACAGACCCATCAAATTCAGAGAGGTTCTGCTTAGGTTTACTCTCCAATGTTAAC
  CGAAATGCCACGGTAGAAATGACAAGAAGGCATATAGGAAGAGGAGTGCGCTTATACTAC
  ATAGGTGGGGAAGTTTTTGCTGAGTGCCTAAGTGATAGTGCAATCTTTGTGCAGAGCCCC
  AATTGTAATCAGAGATATGGCTGGCACCCTGCAACAGTGTGTAAAATTCCACCAGGCTGT
  AATCTGAAGATCTTCAACAACCAGGAATTTGCTGCTCTTCTGGCTCAGTCTGTTAATCAG
  GGTTTTGAAGCCGTCTATCAGCTAACTAGAATGTGCACCATAAGAATGAGTTTTGTGAAA
  GGGTGGGGAGCAGAATACCGAAGGCAGACGGTAACAAGTACTCCTTGCTGGATTGAACTT
  CATCTGAATGGACCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCA
  GTGCGTTGCTCAAGCATGTCATAA
  
  Clone variation with respect to NM_005901.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005901 unedited
  TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTTGGAACCTAACCA
  TGCTGGTACCCTGATCTCGGACTTGCAGCCTCCGGAACTGTTCGATACAAGAGGCTGTTT
  TCCTAGCGTGGCTTGCTGCCTTTGGTAAGAACATGTCGTCCATCTTGCCATTCACGCCGC
  CAGTTGTGAAGAGACTGCTGGGATGGAAGAAGTCAGCTGGTGGGTCTGGAGGAGCAGGCG
  GAGGAGAGCAGAATGGGCAGGAAGAAAAGTGGTGTGAGAAAGCAGTGAAAAGTCTGGTGA
  AGAAGCTAAAGAAAACAGGACGATTAGATGAGCTTGAGAAAGCCATCACCACTCAAAACT
  GTAATACTAAATGTGTTACCATACCAAGCACTTGCTCTGAAATTTGGGGACTGAGTACAC
  CAAATACGATAGATCAGTGGGATACAACAGGCCTTTACAGCTTCTCTGAACAAACCAGGT
  CTCTTGATGGTCGTCTCCAGGTATCCCATCGAAAAGGATTGCCACATGTTATATATTGCC
  GATTATGGCGCTGGCCTGATCTTCACAGTCATCATGAACTCAAGGCAATTGAAAACTGCG
  AATATGCTTTTAATCTTAAAAAGGATGAAGTATGTGTAAACCCTTACCACTATCAGAGAG
  TTGAGACACCAGTTTTGCCTCCAGTATTAGTGCCCCGACACACCGAGATCCTAACAGAAC
  TTCCGCCTCTGGATGACTATACTCACTCCATTCCAGAAAACACTACTTTCCAGCANGAAT
  TGAGCCACAGAGTATTATATTCCAGAAACGCCACCTNCTGGATATATCAGTGGAGATGGA
  GAAACAAGTGACCCACAGTTGAATCAAGTATGGACACAGGCTCTCAGCAGAACTTCTCCT
  ACTACTCTTTCCCCTGTNNATCTAGCTTGGATTACAGNCAGTTACTACTCAGACCTGCAT
  TTTGGGGGTCGATAGCTATATGAATAATCAAAGGGTGGAAANCCTTCTGCTCACAGCCTA
  CTACTGTAAGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005901 unedited
  NNCCTTACTTGNCCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTCAGCTT
  CAAAAATTGAAAATTTATGAAAAGAGACCAGGAGTGGTTATTTTCCATTTCTACTAAGAT
  GCAAACAAGAAGAAATGTTTAAACTGCAATGAGTCAACATCAGACATAATAATTATTTGC
  TGCAGAATGAACTAGCAACAGAGTTTCGGAAATCTCCTCTCACTACAGAGCATGAAATAT
  TGTCAATCACAAAACCAGGATCAGTTAAGAACAGCTCAGACTGCAGGTAACTTATAAGCT
  AAAAAACTTGCTAAATAAATGGCTTTTCCCCCAATTTTTAAAATTCTGAATTCATAATTA
  TGTGTAGAATAACAATCAATTTATAAACTGAATACAACATACTTTAAAAACAGATGGCCC
  ATATCTGCTGATCCTACCTCAGCATCACTTTTCTAGGTATTTTGCAGAAACAATTACATT
  TACAAAAGGCCCATTTCATAATGCAATCTGGTTACTAAACCAAATCAAACTACTCGAAGA
  GCAGAAGGTCTGCTGTTGAAATATTGTAAAATACCTTTGGAAAAACGGTATTTATAGATT
  AGCTTTTTTAAAAAGGGATAAATATGTGGAAGTAATTCTAGCATATCCCTGAAAAAATTA
  TACTGCCACCTATGCATTTTTTATGAGGTGGAAGGATAAATCAACAGACTGCTAGATTAT
  ACAAGCCAATTATTCCTGTTGAAATTTGGGCATAAGACAAAGGGACTTTCTCCATATGAA
  TTCTTACTGTATCCCAGAATTACTGGTGCCAGCAGTATCAATGCAACTATTACTGGNNGA
  TGATGTCATGTTATGCTGTTTTGATTATGAGGACAAAATGGGAATGGGAAAAAAAGGGCT
  TGNAAAGGNTTTCCGNTTGGGTTCCCTATTATAGATTAACAGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005901
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005901.3, NP_005892.1
• RefSeq Size: 5415 bp
• RefSeq ORF: 1404 bp
• Locus ID: 4087
• Cytogenetics: 18q21.1
• Domains: DWB, DWA, MH1
• Protein Families: Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors
• Protein Pathways: Adherens junction, Cell cycle, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway
• Gene Summary: 'The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]'
  Transcript Variant: This variant (1) uses an alternate exon (1b) in the 5' UTR compared to variant 2. Both variants 1 and 2 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.