SMAD2 (NM_005901) Human Untagged Clone

CAT#: SC116434

SMAD2 (untagged)-Human SMAD family member 2 (SMAD2), transcript variant 1


  "NM_005901" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol SMAD2
Synonyms hMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC116434 sequence for NM_005901 edited (data generated by NextGen Sequencing)
ATGTCGTCCATCTTGCCATTCACGCCGCCAGTTGTGAAGAGACTGCTGGGATGGAAGAAG
TCAGCTGGTGGGTCTGGAGGAGCAGGCGGAGGAGAGCAGAATGGGCAGGAAGAAAAGTGG
TGTGAGAAAGCAGTGAAAAGTCTGGTGAAGAAGCTAAAGAAAACAGGACGATTAGATGAG
CTTGAGAAAGCCATCACCACTCAAAACTGTAATACTAAATGTGTTACCATACCAAGCACT
TGCTCTGAAATTTGGGGACTGAGTACACCAAATACGATAGATCAGTGGGATACAACAGGC
CTTTACAGCTTCTCTGAACAAACCAGGTCTCTTGATGGTCGTCTCCAGGTATCCCATCGA
AAAGGATTGCCACATGTTATATATTGCCGATTATGGCGCTGGCCTGATCTTCACAGTCAT
CATGAACTCAAGGCAATTGAAAACTGCGAATATGCTTTTAATCTTAAAAAGGATGAAGTA
TGTGTAAACCCTTACCACTATCAGAGAGTTGAGACACCAGTTTTGCCTCCAGTATTAGTG
CCCCGACACACCGAGATCCTAACAGAACTTCCGCCTCTGGATGACTATACTCACTCCATT
CCAGAAAACACTAACTTCCCAGCAGGAATTGAGCCACAGAGTAATTATATTCCAGAAACG
CCACCTCCTGGATATATCAGTGAAGATGGAGAAACAAGTGACCAACAGTTGAATCAAAGT
ATGGACACAGGCTCTCCAGCAGAACTATCTCCTACTACTCTTTCCCCTGTTAATCATAGC
TTGGATTTACAGCCAGTTACTTACTCAGAACCTGCATTTTGGTGTTCGATAGCATATTAT
GAATTAAATCAGAGGGTTGGAGAAACCTTCCATGCATCACAGCCCTCACTCACTGTAGAT
GGCTTTACAGACCCATCAAATTCAGAGAGGTTCTGCTTAGGTTTACTCTCCAATGTTAAC
CGAAATGCCACGGTAGAAATGACAAGAAGGCATATAGGAAGAGGAGTGCGCTTATACTAC
ATAGGTGGGGAAGTTTTTGCTGAGTGCCTAAGTGATAGTGCAATCTTTGTGCAGAGCCCC
AATTGTAATCAGAGATATGGCTGGCACCCTGCAACAGTGTGTAAAATTCCACCAGGCTGT
AATCTGAAGATCTTCAACAACCAGGAATTTGCTGCTCTTCTGGCTCAGTCTGTTAATCAG
GGTTTTGAAGCCGTCTATCAGCTAACTAGAATGTGCACCATAAGAATGAGTTTTGTGAAA
GGGTGGGGAGCAGAATACCGAAGGCAGACGGTAACAAGTACTCCTTGCTGGATTGAACTT
CATCTGAATGGACCTCTACAGTGGTTGGACAAAGTATTAACTCAGATGGGATCCCCTTCA
GTGCGTTGCTCAAGCATGTCATAA

Clone variation with respect to NM_005901.4
>OriGene 5' read for NM_005901 unedited
TTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTTGGAACCTAACCA
TGCTGGTACCCTGATCTCGGACTTGCAGCCTCCGGAACTGTTCGATACAAGAGGCTGTTT
TCCTAGCGTGGCTTGCTGCCTTTGGTAAGAACATGTCGTCCATCTTGCCATTCACGCCGC
CAGTTGTGAAGAGACTGCTGGGATGGAAGAAGTCAGCTGGTGGGTCTGGAGGAGCAGGCG
GAGGAGAGCAGAATGGGCAGGAAGAAAAGTGGTGTGAGAAAGCAGTGAAAAGTCTGGTGA
AGAAGCTAAAGAAAACAGGACGATTAGATGAGCTTGAGAAAGCCATCACCACTCAAAACT
GTAATACTAAATGTGTTACCATACCAAGCACTTGCTCTGAAATTTGGGGACTGAGTACAC
CAAATACGATAGATCAGTGGGATACAACAGGCCTTTACAGCTTCTCTGAACAAACCAGGT
CTCTTGATGGTCGTCTCCAGGTATCCCATCGAAAAGGATTGCCACATGTTATATATTGCC
GATTATGGCGCTGGCCTGATCTTCACAGTCATCATGAACTCAAGGCAATTGAAAACTGCG
AATATGCTTTTAATCTTAAAAAGGATGAAGTATGTGTAAACCCTTACCACTATCAGAGAG
TTGAGACACCAGTTTTGCCTCCAGTATTAGTGCCCCGACACACCGAGATCCTAACAGAAC
TTCCGCCTCTGGATGACTATACTCACTCCATTCCAGAAAACACTACTTTCCAGCANGAAT
TGAGCCACAGAGTATTATATTCCAGAAACGCCACCTNCTGGATATATCAGTGGAGATGGA
GAAACAAGTGACCCACAGTTGAATCAAGTATGGACACAGGCTCTCAGCAGAACTTCTCCT
ACTACTCTTTCCCCTGTNNATCTAGCTTGGATTACAGNCAGTTACTACTCAGACCTGCAT
TTTGGGGGTCGATAGCTATATGAATAATCAAAGGGTGGAAANCCTTCTGCTCACAGCCTA
CTACTGTAAGA
>OriGene 3' read for NM_005901 unedited
NNCCTTACTTGNCCGCGGCCGCAATCTANGATCGAGTTTTTTTTTTTTTTTTTTCAGCTT
CAAAAATTGAAAATTTATGAAAAGAGACCAGGAGTGGTTATTTTCCATTTCTACTAAGAT
GCAAACAAGAAGAAATGTTTAAACTGCAATGAGTCAACATCAGACATAATAATTATTTGC
TGCAGAATGAACTAGCAACAGAGTTTCGGAAATCTCCTCTCACTACAGAGCATGAAATAT
TGTCAATCACAAAACCAGGATCAGTTAAGAACAGCTCAGACTGCAGGTAACTTATAAGCT
AAAAAACTTGCTAAATAAATGGCTTTTCCCCCAATTTTTAAAATTCTGAATTCATAATTA
TGTGTAGAATAACAATCAATTTATAAACTGAATACAACATACTTTAAAAACAGATGGCCC
ATATCTGCTGATCCTACCTCAGCATCACTTTTCTAGGTATTTTGCAGAAACAATTACATT
TACAAAAGGCCCATTTCATAATGCAATCTGGTTACTAAACCAAATCAAACTACTCGAAGA
GCAGAAGGTCTGCTGTTGAAATATTGTAAAATACCTTTGGAAAAACGGTATTTATAGATT
AGCTTTTTTAAAAAGGGATAAATATGTGGAAGTAATTCTAGCATATCCCTGAAAAAATTA
TACTGCCACCTATGCATTTTTTATGAGGTGGAAGGATAAATCAACAGACTGCTAGATTAT
ACAAGCCAATTATTCCTGTTGAAATTTGGGCATAAGACAAAGGGACTTTCTCCATATGAA
TTCTTACTGTATCCCAGAATTACTGGTGCCAGCAGTATCAATGCAACTATTACTGGNNGA
TGATGTCATGTTATGCTGTTTTGATTATGAGGACAAAATGGGAATGGGAAAAAAAGGGCT
TGNAAAGGNTTTCCGNTTGGGTTCCCTATTATAGATTAACAGT
Restriction Sites NotI-NotI     
ACCN NM_005901
Insert Size 3000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005901.3, NP_005892.1
RefSeq Size 5415 bp
RefSeq ORF 1404 bp
Locus ID 4087
Cytogenetics 18q21.1
Domains DWB, DWA, MH1
Protein Families Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors
Protein Pathways Adherens junction, Cell cycle, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway
Gene Summary 'The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]'
Transcript Variant: This variant (1) uses an alternate exon (1b) in the 5' UTR compared to variant 2. Both variants 1 and 2 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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