SMAD5 (NM_005903) Human Untagged Clone

CAT#: SC116436

SMAD5 (untagged)-Human SMAD family member 5 (SMAD5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SMAD5
• Synonyms: DWFC; JV5-1; MADH5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005903, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACGTCAATGGCCAGCTTGTTTTCTTTTACTAGTCCAGCAGTAAAGCGATTGTTGGGCTGGAAACAAG
  GTGATGAGGAGGAGAAATGGGCAGAAAAGGCAGTTGATGCTTTGGTGAAGAAACTAAAAAAGAAAAAGGG
  TGCCATGGAGGAACTGGAGAAAGCCTTGAGCAGTCCAGGACAGCCGAGTAAATGTGTCACTATTCCCAGA
  TCTTTAGATGGACGCCTGCAGGTTTCTCACAGAAAAGGCTTACCCCATGTTATATATTGTCGTGTTTGGC
  GCTGGCCGGATTTGCAGAGTCATCATGAGCTAAAGCCGTTGGATATTTGTGAATTTCCTTTTGGATCTAA
  GCAAAAAGAAGTTTGTATCAACCCATACCACTATAAGAGAGTGGAGAGTCCAGTCTTACCTCCAGTATTA
  GTGCCTCGTCATAATGAATTCAATCCACAACACAGCCTTCTGGTTCAGTTTAGGAACCTGAGCCACAATG
  AACCACACATGCCACAAAATGCCACGTTTCCAGATTCTTTCCACCAGCCCAACAACACTCCTTTTCCCTT
  ATCTCCAAACAGCCCTTATCCCCCTTCTCCTGCTAGCAGCACATATCCCAACTCCCCAGCAAGTTCTGGA
  CCAGGAAGTCCATTTCAGCTCCCAGCTGATACGCCTCCTCCTGCCTATATGCCACCTGATGATCAGATGG
  GTCAAGATAATTCCCAGCCTATGGATACAAGCAATAATATGATTCCTCAGATTATGCCCAGTATATCCAG
  CAGGGATGTTCAGCCTGTTGCCTATGAAGAGCCTAAACATTGGTGTTCAATAGTCTACTATGAATTAAAC
  AATCGTGTTGGAGAAGCTTTTCATGCATCTTCTACTAGTGTGTTAGTAGATGGATTCACAGATCCTTCAA
  ATAACAAAAGTAGATTCTGCTTGGGTTTGTTGTCAAATGTTAATCGTAATTCGACAATTGAAAACACTAG
  GCGACATATTGGAAAAGGTGTTCATCTGTACTATGTTGGTGGAGAGGTGTATGCGGAATGCCTCAGTGAC
  AGCAGCATATTTGTACAGAGTAGGAACTGCAACTTTCATCATGGCTTTCATCCCACCACTGTCTGTAAGA
  TTCCCAGCAGCTGCAGCCTCAAAATTTTTAACAATCAGGAGTTTGCTCAGCTTCTGGCTCAATCTGTCAA
  CCATGGGTTTGAGGCAGTATATGAGCTCACCAAAATGTGTACCATTCGGATGAGTTTTGTCAAGGGTTGG
  GGAGCAGAATATCACCGGCAGGATGTAACCAGCACCCCATGTTGGATTGAGATTCATCTTCATGGGCCTC
  TTCAGTGGCTGGATAAAGTCCTTACTCAGATGGGCTCCCCTCTGAACCCCATATCTTCTGTTTCATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005903 unedited
  GGTCAAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGNANAAGCG
  CAGCGACGGCGTCGGGAGAGCGCGCCTAGCCGGCTCGCGAGACTTGACCCAATGAAAGAA
  GCATATGGCACTTGTGAAGATAAATGTTACTCCTCCCTTTTTAATTGGAACTTCTGCTTA
  GGACCTGTGTATGACGTTTCACCTGTGATCTGTTCTTTCGGTAGCCACTGACTTTGAGTT
  ACAGGAAGGTCTCCGAAGATTTGTGTCAAATGACGTCAATGGCCAGCTTGTTTTCTTTTA
  CTAGTCCAGCAGTAAAGCGATTGTTGGGCTGGAAACAAGGTGATGAGGAGGAGAAATGGG
  CAGAAAAGGCAGTTGATGCTTTGGTGAAGAAACTAAAAAAGAAAAAGGGTGCCATGGAGG
  AACTGGAGAAAGCCTTGAGCAGTCCAGGACAGCCGAGTAAATGTGTCACTATTCCCAGAT
  CTTTAGATGGACGCCTGCAGGTTTCTCACAGAAAAGGCTTACCCCATGTTATATATTGTC
  GTGTTTGGCGCTGGCCGGATTTGCAGAGTCATCATGAGCTAAAGCCGTTGGATATTTGTG
  AATTTCCTTTTGGATCTAAGCAAAAAGAAGTTTGTATCAACCCATACCACTATAAGAGAG
  TGGAGAGTCCAGATGTTCAGCCTGTTGCCTATGAAGAGCCTAAACATTGGTGTTCAATAG
  TCTACTATGAATTAAACAATCGTGTTGGAGAAAGCTTTCATGCATCTTCTACTAGTGTGT
  TAGTAGATGGATTCACAGATCCTTCAAATAACANAGTAGATTCTGCTTGGGTTTTGTTGT
  CAAATGTTAATCGTAATTCGACAATTGAAAACACTANGCGACATATTGGNAAAAGTGTTC
  ATCTGTACTATGNT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005903 unedited
  GCGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTCCAAAATAATTTCTTCATTC
  TCATGTCATAATTAAGTGTCCATTTGACAGCTCCTGAGAAAAAAACTGGTTCAAAGTTCT
  TTATCCAAAGCCAAAAACATGATTGATTAAATGGATATTTTGGAGTTCGTTCATGTCTTT
  TCTCTCTAAAGATTGTGTTTTGGGAGACTAGCTTCCACATAAGTACTTAAAAAATGGAAA
  AAGAGGATCATCGATAAATGAGAGAAGAGTCTATATTCGTTGATGGGTGAGTGTTTTGTT
  TTGTTTTGTTTTTTGAGGCAGAGTCTCGCTCTGTCGCCCAGGCTGGAGTGCAGTGGTGCG
  ATCTCGGCTCACCACAACCTCCGCCTCCCAGGTTCAAGTGATTCTTCTGCCTCAGCTTCC
  CGAGTAGCTGGGACTTCAGGCACGCACCACCATGCCCAGCTAATTTTTGTATTTTTTGTA
  GAGACAGGGTTTCACCATATTGGCCAGGCTGGTCTCAAACTCCTGACATCATGATCTGCC
  TGCCTCAGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCACTGTGCCCAGCCGAGTGT
  TTTAAAATCAGCTATCCCTACATCCACTGCTGATTCATTTAGTATCAAATCAGTACCCTT
  ATCCTTGGGGCCCAACTGAAGACCCTCCTACGGCTCCAGTGCATAGGTCCTTGCGTAGGG
  AAGCTGATACAAGTAAGCTGCCACCTGTACACACCATGGAATTACCACTTGATCCTCCAC
  CCTGAAAACCCCCTAAAACGTAGGGATTAAAAAGCCCTAAGTTGGACCTTTGAATACAGT
  CTTTTACCCAGGTGAAAGGAACCAAACCCAAAAAGCCTACTTCTCCAAGTGACCTTTAAT
  GCAGGTTGGTATCCAAAAGGGCCCGGCCTAAGTTAACCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005903
• Insert Size: 3110 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005903.5, NP_005894.3
• RefSeq Size: 7012 bp
• RefSeq ORF: 1398 bp
• Locus ID: 4090
• Cytogenetics: 5q31.1
• Domains: DWB, DWA, MH1
• Protein Families: Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors
• Protein Pathways: TGF-beta signaling pathway
• Gene Summary: 'The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]'
  Transcript Variant: This variant (1) differs in the 5' UTR, compared to variant 2. Variants 1-3 encode the same protein.