MADH7 (SMAD7) (NM_005904) Human Untagged Clone

CAT#: SC116437

SMAD7 (untagged)-Human SMAD family member 7 (SMAD7), transcript variant 1


  "NM_005904" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

Product Images

Other products for "SMAD7"

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol SMAD7
Synonyms CRCS3; MADH7; MADH8
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC116437 sequence for NM_005904 edited (data generated by NextGen Sequencing)
ATGTTCAGGACCAAACGATCTGCGCTCGTCCGGCGTCTCTGGAGGAGCCGTGCGCCCGGC
GGCGAGGACGAGGAGGAGGGCGCAGGGGGAGGTGGAGGAGGAGGCGAGCTGCGGGGAGAA
GGGGCGACGGACAGCCGAGCGCATGGGGCCGGTGGCGGCGGCCCGGGCAGGGCTGGATGC
TGCCTGGGCAAGGCGGTGCGAGGTGCCAAAGGTCACCACCATCCCCACCCGCCAGCCGCG
GGCGCCGGCGCGGCCGGGGGCGCCGAGGCGGATCTGAAGGCGCTCACGCACTCGGTGCTC
AAGAAACTGAAGGAGCGGCAGCTGGAGCTGCTGCTCCAGGCCGTGGAGTCCCGCGGCGGG
ACGCGCACCGCGTGCCTCCTGCTGCCCGGCCGCCTGGACTGCAGGCTGGGCCCGGGGGCG
CCCGCCGGCGCGCAGCCTGCGCAGCCGCCCTCGTCCTACTCGCTCCCCCTCCTGCTGTGC
AAAGTGTTCAGGTGGCCGGATCTCAGGCATTCCTCGGAAGTCAAGAGGCTGTGTTGCTGT
GAATCTTACGGGAAGATCAACCCCGAGCTGGTGTGCTGCAACCCCCATCACCTTAGCCGA
CTCTGCGAACTAGAGTCTCCCCCCCCTCCTTACTCCAGATACCCGATGGATTTTCTCAAA
CCAACTGCAGACTGTCCAGATGCTGTGCCTTCCTCCGCTGAAACAGGGGGAACGAATTAT
CTGGCCCCTGGGGGGCTTTCAGATTCCCAACTTCTTCTGGAGCCTGGGGATCGGTCACAC
TGGTGCGTGGTGGCATACTGGGAGGAGAAGACGAGAGTGGGGAGGCTCTACTGTGTCCAG
GAGCCCTCTCTGGATATCTTCTATGATCTACCTCAGGGGAATGGCTTTTGCCTCGGACAG
CTCAATTCGGACAACAAGAGTCAGCTGGTGCAGAAGGTGCGGAGCAAAATCGGCTGCGGC
ATCCAGCTGACGCGGGAGGTGGATGGTGTGTGGGTGTACAACCGCAGCAGTTACCCCATC
TTCATCAAGTCCGCCACACTGGACAACCCGGACTCCAGGACGCTGTTGGTACACAAGGTG
TTCCCCGGTTTCTCCATCAAGGCTTTCGACTACGAGAAGGCGTACAGCCTGCAGCGGCCC
AATGACCACGAGTTTATGCAGCAGCCGTGGACGGGCTTTACCGTGCAGATCAGCTTTGTG
AAGGGCTGGGGCCAGTGCTACACCCGCCAGTTCATCAGCAGCTGCCCGTGCTGGCTAGAG
GTCATCTTCAACAGCCGGTAG

Clone variation with respect to NM_005904.3
>OriGene 5' read for NM_005904 unedited
GCGTAATTCGCAGAGGCGCGCGCCCCGGCCTCCGGGAGACTGGCGCATGCCACGGAGCGC
CCCTCGGGCCGCCGCCGCTCCTGCCCGGGCCCCTGCTGCTGCTGCTGTCGCCTGCGCCTG
CTGCCCCAACTCGGCGCCCGACTTCTTCATGGTGTGCGGAGGTCATGTTCGCTCCTTAGC
AGGCAAACGACTTTTCTCCTCGCCTCCTCGCCCCGCATGTTCAGGACCAAACGATCTGCG
CTCGTCCGGCGTCTCTGGAGGAGCCGTGCGCCCGGCGGCGAGGACGAGGAGGAGGGCGCA
GGGGGAGGTGGAGGAGGAGGCGAGCTGCGGGGAGAAGGGGCGACGGACAGCCGAGCGCAT
GGGGCCGGTGGCGGCGGCCCGGGCAGGGCTGGATGCTGCCTGGGCAAGGCGGTGCGAGGT
GCCAAAGGTCACCACCATCCCCACCCGCCAGCCGCGGGCGCCGGCGCGGCCGGGGGCGCC
GAGGCGGATCTGAAGGCGCTCACGCACTCGGTGCTCAAGAACTGAAGGAGCGGCAGCTGG
AGCTGCTGCTCCAGCCGGTGGAGTCCCGCGGCGGGACGCGCACCGCGTGCCTCCTGCTGC
CCGGCCGCCTGGACTGCANGCTGGCCCCGGGGGGCGCCCGCCGGCGCGCAGNCTGCGCAG
ACGACCCTCGTCCTACTCGCTCCCCCTTCTGCTGTGNAAAGTGGTNCAAGGGGGCCGGAT
CTCAGGCATTCTTCGGAAGTCAAAAGGCTGGGNTTGCTGCGAATCCTACCGGGAAGATCA
ACCCGAACCCTGTGGTGCTGGACCCCCCTCACCTTAACCCGACTCTGCGAACTAAAATCT
CCCCCCCCTCCTTACTTCAGATACCGGGAGTGAATTTCTTAA
>OriGene 3' read for NM_005904 unedited
ACTTGTTTGCTTTTAAAAACGCCTTTGGGCCTAAACTCCTTTATAAAACATCAGCTAGGT
GATAACACCCATAGAAAAAAACACCAATCTTGTGTTTATCTTTTTTAATTTGGCATTTGT
TATTTGCATTTATATTAAAGCAAAGTGCATCTTTTCTTTATTTTTCTTGTTTATACACAT
TGCACAATACATAAATAATGATGCTTATAAAACGTCTTTATATTTACAAGTAATAATATA
TTTATATATAACATAAAATACATTTTTTTCTTTAATAAATCTCAGGTTTTTTTTCAGGAG
TCCTTTCTCTCTCAAAGCACTACAATGCTAAATTTCCAATGAGAATGCTTCTCTTGTTCA
TTTAAACCTTTGTAGTTAGAAAAATAGCTTATGTTAAAGTCTAAACATGCTCATTGAGCT
AAGAACAGTGTCGAAGTATCATACGAGTGTATGAGTTGTAGAAGAAATGAAAGAAGAGTT
AGGTGTCAGCCTAGGATGGTACCTTGGGTTATGACGGACCAAATCCTTCTTGGAGGGAAG
GGGACTGCTAAGCATGTCCCTCCCAAGGACATCCCCGCTTGCTGGCCTAATAGCAGAGCT
TAACACACAGGATGGGAGCAGGCAGTGCTGAGCCTGGTTTCTAAGTCCGGGAAAAACCTG
ATGTCACCAAGGCCAGCCANGGGAAAAGCCTGAAGGGAAAGGCCCACTGGGTTGAGCAAT
ACTGTGAAGGGGCTTGCCCCGGGCACCCCTTGGGAAGCCCATTCTTAGGGAGATCCACGA
ACCAGATTGGCCAAAAAAAAAAGACCCTCCATTGTCAAGGGCTTGGCAGGAAGGAAATAA
AACAAGGGTTGAAATAATTGGTTCTGGGTTGGACACTTTCAAGCCTCCTTGTTCAAAAAC
CTGGGAACAGGCCCCGTCCTTGTTGAGG
Restriction Sites NotI-NotI     
ACCN NM_005904
Insert Size 3000 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005904.2, NP_005895.1
RefSeq Size 3103 bp
RefSeq ORF 1281 bp
Locus ID 4092
Cytogenetics 18q21.1
Domains DWB, DWA, MH1
Protein Families Druggable Genome, Transcription Factors
Protein Pathways TGF-beta signaling pathway
Gene Summary 'The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]'
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

Other Versions

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.