MAT2A (NM_005911) Human Untagged Clone

CAT#: SC116444

MAT2A (untagged)-Human methionine adenosyltransferase II, alpha (MAT2A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAT2A
• Synonyms: MATA2; MATII; SAMS2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116444 sequence for NM_005911 edited (data generated by NextGen Sequencing)
  ATGAACGGACAGCTCAACGGCTTCCACGAGGCGTTCATCGAGGAGGGCACATTCCTTTTC
  ACCTCAGAGTCGGTCGGGGAAGGCCACCCAGATAAGATTTGTGACCAAATCAGTGATGCT
  GTCCTTGATGCCCACCTTCAGCAGGATCCTGATGCCAAAGTAGCTTGTGAAACTGTTGCT
  AAAACTGGAATGATCCTTCTTGCTGGGGAAATTACATCCAGAGCTGCTGTTGACTACCAG
  AAAGTGGTTCGTGAAGCTGTTAAACACATTGGATATGATGATTCTTCCAAAGGTTTTGAC
  TACAAGACTTGTAACGTGCTGGTAGCCTTGGAGCAACAGTCACCAGATATTGCTCAAGGT
  GTTCATCTTGACAGAAATGAAGAAGACATTGGTGCTGGAGACCAGGGCTTAATGTTTGGC
  TATGCCACTGATGAAACTGAGGAGTGTATGCCTTTAACCATTGTCTTGGCACACAAGCTA
  AATGCCAAACTGGCAGAACTACGCCGTAATGGCACTTTGCCTTGGTTACGCCCTGATTCT
  AAAACTCAAGTTACTGTGCAGTATATGCAGGATCGAGGTGCTGTGCTTCCCATCAGAGTC
  CACACAATTGTTATATCTGTTCAGCATGATGAAGAGGTTTGTCTTGATGAAATGAGGGAT
  GCCCTAAAGGAGAAAGTCATCAAAGCAGTTGTGCCTGCGAAATACCTTGATGAGGATACA
  ATCTACCACCTACAGCCAAGTGGCAGATTTGTTATTGGTGGGCCTCAGGGTGATGCTGGT
  TTGACTGGACGCAAAATCATTGTGGACACTTATGGCGGTTGGGGTGCTCATGGAGGAGGT
  GCCTTTTCAGGAAAGGATTATACCAAGGTCGACCGTTCAGCTGCTTATGCTGCTCGTTGG
  GTGGCAAAATCCCTTGTTAAAGGAGGTCTGTGCCGGAGGGTTCTTGTTCAGGTCTCTTAT
  GCTATTGGAGTTTCTCATCCATTATCTATCTCCATTTTCCATTATGGTACCTCTCAGAAG
  AGTGAGAGAGAGCTATTAGAGATTGTGAAGAAGAATTTCGATCTCCGCCCTGGGGTCATT
  GTCAGGGATCTGGATCTGAAGAAGCCAATTTATCAGAGGACTGCAGCCTATGGCCACTTT
  GGTAGGGACAGCTTCCCATGGGAAGTGCCCAAAAAGCTTAAATATTGA
  
  Clone variation with respect to NM_005911.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005911 unedited
  TTTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGGCTACGAGTAGAACGCT
  GTCCGCAGCTTGCGCATTTCGCAGCCGCTGCCGCCTCGCCGCTGCTCCTTCGTAAGGCCA
  CTTCCGCACACCGACACCAACATGAACGGACAGCTCAACGGCTTCCACGAGGCGTTCATC
  GAGGAGGGCACATTCCTTTTCACCTCAGAGTCGGTCGGGGAAGGCCACCCAGATAAGATT
  TGTGACCAAATCAGTGATGCTGTCCTTGATGCCCACCTTCAGCAGGATCCTGATGCCAAA
  GTAGCTTGTGAAACTGTTGCTAAAACTGGAATGATCCTTCTTGCTGGGGAAATTACATCC
  AGAGCTGCTGTTGACTACCAGAAAGTGGTTCGTGAAGCTGTTAAACACATTGGATATGAT
  GATTCTTCCAAAGGTTTTGACTACAAGACTTGTAACGTGCTGGTAGCCTTGGAGCAACAG
  TCACCAGATATTGCTCAAGGTGTTCATCTTGACAGAAATGAAGAAGACATTGGTGCTGGA
  GACCAGGGCTTAATGTTTGGCTATGCCACTGATGAAACTGAGGAGTGTATGCCTTTAACC
  ATTGTCTTGGCACACAAGCTAAATGCCAAACTGGCAGAACTACGCCGTAATGGCACTTTG
  CCTTGGTTACGCCCTGATTCTAAACTCAATTACTGTGCAGATATGCAGGATCGAGGTGCT
  GTGCTTCCATCAGAGTCACACAATTGTATATCTGTTAGCATGTAAAGAAGGTTTGTCTGA
  TGAATGAGGGGATGCCCTAAGGGAGAAGTCTCAAAGCGNGTGGTCCTGCGGAATACCTGA
  TGAGATACATNCTACACCTACAGNCAGTGGNANATTGTTTATGGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005911 unedited
  ATGCACGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAGGTAGAACCTAGC
  ATTTTATTTAGATCTTCATTAAACTGTTGGAATTGAGAACCAGACATACGTAATAAACCT
  CCAAAAATAGATCCTGAAAGGCACTTTCTGCTTAGGGCAAGCAGTCATGGAATAAGCATG
  TAAACAAGCTGGCTTCTCTGTACCACACCAGCCAAGTCAGCTTTCTCCATGGCCAGCCGC
  ACCAGCTCTGCCCTCCCTTCTGTTAACACCAGCCAGACCCCCTGTAGGTCTAACCCAAGG
  TTTTTCTGTAGGACACCTTGGCCTACCTGGGAATGCTGGAAACATGTTGTTAAAGGAATC
  ATGGTGTTGAGAGAAAAAAAAAAAATCTTTTAAAAGCTGCCATCTGAGGTGATGGCTTCT
  CTGTACTTACGCCATACCCCAGAATACAATAAATAAGCAATTAGAAAACGTTCAAGTATG
  AAGGGATTTCCTCCTCCCCGCCAAAAGCACTGCTCTCTGAAGGAAGCTGGTTTCTCTGTA
  CCTACACCAGCTGTTCAAAAAGCTCATTGGACCTGGTTTTGAAAATAAAACAAAGTTAAA
  ACCCTGGGAGGAGTTATTGTGCAGTGTGGAGTACTCAGGCTTTCTTATAAAGAAAAAAAA
  AGTTATCTGGTACCAAAGTGTGCACCCTACAGACCCCTCAGTACTGCCCTGTGACTTCTC
  TGTATGACATCACAAGGCTGCCAAGTGCCTGTTTTTCTAGACTAAGAGTTGNTGAGGTTT
  GCTAGTGCTGAAACATGCATAGAATTGGTTACTAACTAAAACCTTATACGTACGTCTCCA
  AAGAAGCTGAAAGTGTAGAGATTTGATTTGCGCTGTTAGAGTGGCATTACGACATATCCC
  CAGGGAGAGGTTAATGGTACTGCCTCTTTGAGCCAGGCTGAGCTCCACTGGAACTTGGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005911
• Insert Size: 2820 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005911.4, NP_005902.1
• RefSeq Size: 2835 bp
• RefSeq ORF: 1188 bp
• Locus ID: 4144
• Cytogenetics: 2p11.2
• Domains: S-AdoMet_synt
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways, Selenoamino acid metabolism
• Gene Summary: 'The protein encoded by this gene catalyzes the production of S-adenosylmethionine (AdoMet) from methionine and ATP. AdoMet is the key methyl donor in cellular processes. [provided by RefSeq, Jun 2011]'