NET1 (NM_005863) Human Untagged Clone

CAT#: SC116508

NET1 (untagged)-Human neuroepithelial cell transforming 1 (NET1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NET1
• Synonyms: ARHGEF8; NET1A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116508 sequence for NM_005863 edited (data generated by NextGen Sequencing)
  ATGGTGGCACATGATGAGACTGGAGGTCTCCTACCTATTAAAAGGACCATACGAGTCCTA
  GATGTCAATAACCAGTCCTTCAGAGAACAAGAGGAGCCAAGCAATAAAAGAGTTCGACCT
  CTGGCTCGTGTCACGTCCTTGGCAAATTTAATCTCTCCTGTAAGAAATGGAGCTGTCAGA
  CGTTTTGGTCAAACAATACAGTCATTTACCCTTCGTGGTGACCACAGATCCCCAGCCTCT
  GCCCAGAAGTTTTCTAGCAGGTCAACAGTCCCAACACCCGCCAAGAGAAGGAGCAGTGCA
  CTGTGGTCAGAGATGCTGGACATCACCATGAAGGAGTCTCTCACCACCAGGGAGATCAGA
  CGGCAGGAGGCAATATATGAAATGTCCCGAGGTGAACAGGATTTAATTGAGGATCTCAAA
  CTTGCAAGAAAGGCCTACCATGACCCCATGTTAAAGTTGTCCATCATGTCAGAAGAGGAA
  CTCACACATATATTTGGTGATCTGGACTCTTACATACCTCTGCATGAAGATTTGTTGACA
  AGAATAGGAGAAGCAACCAAGCCTGATGGAACAGTGGAGCAGATTGGTCACATTCTCGTG
  AGCTGGTTACCGCGCTTGAATGCCTACAGAGGTTACTGTAGTAACCAGCTGGCAGCCAAA
  GCTCTTCTTGATCAAAAGAAACAGGATCCAAGAGTCCAAGACTTCCTCCAGCGATGTCTC
  GAGTCTCCCTTCAGTCGAAAACTAGATCTTTGGAGTTTCCTAGATATCCCTCGAAGTCGC
  CTAGTCAAATACCCTTTACTGTTAAAAGAAATTCTTAAACACACTCCAAAAGAGCACCCT
  GATGTTCAGCTTCTGGAGGATGCTATATTGATAATACAGGGAGTCCTCTCTGATATCAAC
  TTGAAGAAAGGTGAATCCGAGTGCCAGTATTACATCGACAAGCTGGAGTACCTGGATGAA
  AAGCAGAGGGACCCCAGAATCGAAGCGAGCAAAGTGCTGCTGTGCCATGGGGAGCTGCGG
  AGCAAGAGTGGACATAAACTTTACATTTTCCTGTTTCAAGACATCTTGGTTCTGACTCGG
  CCCGTCACACGGAACGAACGGCACTCTTACCAGGTTTACCGGCAGCCAATCCCAGTCCAA
  GAGCTAGTCCTAGAAGACCTGCAGGATGGAGATGTGAGAATGGGAGGCTCCTTTCGAGGA
  GCTTTCAGTAACTCAGAGAAAGCTAAAAATATCTTTAGAATTCGCTTCCATGACCCCTCT
  CCAGCCCAGTCTCACACTCTGCAAGCCAATGACGTGTTCCACAAGCAGCAGTGGTTCAAC
  TGTATTCGAGCGGCCATTGCCCCCTTCCAGTCGGCAGGCAGTCCACCTGAGCTGCAGGGC
  CTGCCGGAGCTGCACGAAGAGTGTGAGGGGAACCACCCCTCTGCGAGGAAACTCACAGCC
  CAGAGGAGGGCATCCACAGTTTCCAGTGTTACTCAGGTAGAAGTTGATGAAAACGCTTAC
  AGATGTGGCTCTGGCATGCAGATGGCAGAGGACAGCAAGAGCTTAAAGACACACCAGACA
  CAGCCCGGCATCCGAAGAGCGAGGGACAAAGCCCTTTCTGGTGGCAAACGGAAAGAGACT
  TTGGTGTAG
  
  Clone variation with respect to NM_005863.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005863 unedited
  GAAAACCCATTCGGCACGAGGCCGCGGCGATAGGCATGGGCACGTGGCTGCCGAGGTGGC
  CGAGCTCTGGGAAGAAAAGCCCGTGTGCCTCTGCATAGCGTCGCTACAGCGCTGACTCGG
  TGTGGATTGATTGGAAAGGTTTGAGGGAGTACTTGGGAAGCATGGTGGCACATGATGATA
  TTGTTGTCTCCTACCTATTAAAAGGACCATACGAGTCCTAGATGTCAATAACCAGTCCTT
  CAGAGAACAAGAGGAGCCAAGCAATAAAAGAGTTCGACCTCTGGCTCGTGTCACGTCCTT
  GGCAAATTTAATCTCTCCTGTAAGAAATGGAGCTGTCAGACGTTTTGGTCAAACAATACA
  GTCATTTACCCTTCGTGGTGACCACAGATCCCCAGCCTCTGCCCAGAAGTTTTCTAGCAG
  GTCAACAGTCCCAACACCCGCCAAGAGAAGGAGCAGTGCACTGTGGTCAGAGATGCTGGA
  CATCACCATGAAGGAGTCTCTCACCACCAGGGAGATCAGACGGCAGGAGGCAATATATGA
  AATGTCCCGAGGTGAACAGGATTTAATTGAGGATCTCAAACTTGCAAGAAAGGCCTACCA
  TGACCCCATGTTAAAGTTGTCCATCATGTCAGAAGAGGAACTCACACATATATTTGGTGA
  TCTGGACTCTTACATACCTCTGCATGAAGATTTGTTGACNAGAATAGGAGAAGCAACCAA
  GCCTGATGGAACAGTGGAGCAGATTGGTCACATTCTCGTGAGCTGGTTACCGCGCTTTGA
  TGCCTACAGAGGTTACTGTAGTAACCAGCTGGCAGCCAAAGCTCTTTCTGATCAANNAGA
  ACAGATCCAAGAGTCCAAGACTTCCTCCAGCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005863 unedited
  TAGAGTCGAGTTTTTTTTTTTTTTTTTTACACACACATTTTTAATCAGGAAGAAAAATAC
  TTTAAAGACATGCCAATTTGAAAAGGCATCAAAGTAAAAAAATAAAAGCAAATGCTAAAA
  ACTACTTTACAATAAAAAAATTAAATAATCGGCAGGTTAAATGAATGTAAAATGAGGAAT
  GTACAGTGAAAAACAAACTAATATAAAGCATTCCAGTTGATAAAAACCTCCTCAGGCTTA
  TGGTTTGTTTTCCAAGGAAATTATGTTTCAATGTAAAGTTTGAAATACTCCAGACATACA
  TTCCATGTAGGTTTTGGGTGCCAATGTTAAAATTTCAAATTTTGCATGCAAGGCTTAGCA
  AAGAAACACTGGCAGAATTCCAGCATTTGCAAAATTCTAAGTTTTGGTGAATATTGTAAA
  TATTACAATTGGTATTAGAAAGCCATGATGAATCCAGAATTAAGAGAAAACCCATTTCAT
  AAATATTTTGTTTGATTAAAAAATACCAGGCTTACCATGTTCTAAATAATTCAAGAAAAA
  TATCTTTAAAAAAAAAGGGACTGCATTTACAGTAATCTGTATATCTTTAGCTGCCATTAA
  AAAAAGAANAAAGAACACCCANAAACANTGAAAATGTTACAACTGGTATAAAGTAACCCT
  ATGATGCTNCCCTTACGAGAAAACAAACTGTACACATTTATAAACAAACAGTCTCTCCCA
  TCAGTTTACACACAGAGCCTTTCTCTACACAAAGTCTCTTTCCGTTTTGCACAGAAAAGG
  GCTTGTCCNTCGCTCTTCGGATGCCNGGCTGGGTCTGGNGNGCCTTAAGCTTTTGCTGCC
  TTTGCATTNGATGCAGAGCCCATTGGAAACGTTTTATACTTCTCCTGAGTACCTGGAACT
  GGGATGCCTCTTTGGCTGGAGTTTCTGCAAAGGGGGGTCCCCTAATTTTTTGCACTCCGA
  GCCTGATTTAGTGACTCCTCCCATGAGGGCATGCCNTTAATCATGACCTGTGGTGGAACC
  T
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005863
• ORF Size: 1629 bp
• Insert Size: 2620
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).

Reference Data

• RefSeq: NM_005863.2, NP_005854.2
• RefSeq Size: 3236
• RefSeq ORF: 1629
• Locus ID: 10276
• Domains: RhoGEF, PH
• Protein Families: Druggable Genome
• Gene Summary: This gene is part of the family of Rho guanine nucleotide exchange factors. Members of this family activate Rho proteins by catalyzing the exchange of GDP for GTP. The protein encoded by this gene interacts with RhoA within the cell nucleus and may play a role in repairing DNA damage after ionizing radiation. Pseudogenes of this gene are located on the long arms of chromosomes 1, 7 and 18. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.